{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T07:04:00Z","timestamp":1777878240066,"version":"3.51.4"},"publisher-location":"New York, NY","reference-count":100,"publisher":"Springer US","isbn-type":[{"value":"9781071650301","type":"print"},{"value":"9781071650318","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5031-8_29","type":"book-chapter","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T23:15:10Z","timestamp":1777590910000},"page":"335-360","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metabolic Engineering of Microalgae for Biofuel Production"],"prefix":"10.1007","author":[{"given":"Mohammad Pooya","family":"Naghshbandi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meisam","family":"Tabatabaei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mortaza","family":"Aghbashlo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Nauman","family":"Aftab","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irfana","family":"Iqbal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,4,1]]},"reference":[{"key":"29_CR1","doi-asserted-by":"publisher","first-page":"1376","DOI":"10.3389\/fmicb.2015.01376","volume":"6","author":"JA Gimpel","year":"2015","unstructured":"Gimpel JA, Henr\u00edquez V, Mayfield SP (2015) In metabolic engineering of eukaryotic microalgae: potential and challenges come with great diversity. Front Microbiol 6:1376. https:\/\/doi.org\/10.3389\/fmicb.2015.01376","journal-title":"Front Microbiol"},{"key":"29_CR2","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1038\/nbt0210-126","volume":"28","author":"E Stephens","year":"2010","unstructured":"Stephens E, Ross IL, King Z et al (2010) An economic and technical evaluation of microalgal biofuels. Nat Biotechnol 28:126\u2013128. https:\/\/doi.org\/10.1038\/nbt0210-126","journal-title":"Nat Biotechnol"},{"key":"29_CR3","doi-asserted-by":"publisher","unstructured":"Peng K, Li J, Jiao K et al (2018) The bioeconomy of microalgal biofuels. In: Energy from microalgae. Springer, Cham, pp 157\u2013169. https:\/\/doi.org\/10.1007\/978-3-319-69093-3_7","DOI":"10.1007\/978-3-319-69093-3_7"},{"key":"29_CR4","doi-asserted-by":"publisher","first-page":"3541","DOI":"10.1016\/j.renene.2011.04.031","volume":"36","author":"JJ Cheng","year":"2011","unstructured":"Cheng JJ, Timilsina GR (2011) Status and barriers of advanced biofuel technologies: a review. Renew Energy 36:3541\u20133549. https:\/\/doi.org\/10.1016\/j.renene.2011.04.031","journal-title":"Renew Energy"},{"key":"29_CR5","doi-asserted-by":"publisher","first-page":"2596","DOI":"10.1016\/j.rser.2010.06.014","volume":"14","author":"J Singh","year":"2010","unstructured":"Singh J, Gu S (2010) Commercialization potential of microalgae for biofuels production. Renew Sustain Energy Rev 14:2596\u20132610. https:\/\/doi.org\/10.1016\/j.rser.2010.06.014","journal-title":"Renew Sustain Energy Rev"},{"key":"29_CR6","doi-asserted-by":"publisher","first-page":"432","DOI":"10.3389\/fmicb.2016.00432","volume":"7","author":"C Banerjee","year":"2016","unstructured":"Banerjee C, Dubey KK, Shukla P (2016) Metabolic engineering of microalgal based biofuel production: prospects and challenges. Front Microbiol 7:432. https:\/\/doi.org\/10.3389\/fmicb.2016.00432","journal-title":"Front Microbiol"},{"key":"29_CR7","doi-asserted-by":"publisher","first-page":"1005","DOI":"10.1016\/0196-8904(93)90048-F","volume":"34","author":"LM Brown","year":"1993","unstructured":"Brown LM, Zeiler KG (1993) Aquatic biomass and carbon dioxide trapping. Energy Convers Manag 34:1005\u20131013. https:\/\/doi.org\/10.1016\/0196-8904(93)90048-F","journal-title":"Energy Convers Manag"},{"key":"29_CR8","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1016\/j.tibtech.2007.12.002","volume":"26","author":"Y Chisti","year":"2008","unstructured":"Chisti Y (2008) Biodiesel from microalgae beats bioethanol. Trends Biotechnol 26:126\u2013131. https:\/\/doi.org\/10.1016\/j.tibtech.2007.12.002","journal-title":"Trends Biotechnol"},{"key":"29_CR9","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1007\/s12155-008-9008-8","volume":"1","author":"PM Schenk","year":"2008","unstructured":"Schenk PM, Thomas-Hall SR, Stephens E et al (2008) Second generation biofuels: high-efficiency microalgae for biodiesel production. Bioenergy Res 1:20\u201343. https:\/\/doi.org\/10.1007\/s12155-008-9008-8","journal-title":"Bioenergy Res"},{"key":"29_CR10","doi-asserted-by":"publisher","first-page":"703","DOI":"10.1098\/rsif.2009.0322","volume":"7","author":"HC Greenwell","year":"2010","unstructured":"Greenwell HC, Laurens LML, Shields RJ et al (2010) Placing microalgae on the biofuels priority list: a review of the technological challenges. J R Soc Interface 7:703\u2013726. https:\/\/doi.org\/10.1098\/rsif.2009.0322","journal-title":"J R Soc Interface"},{"key":"29_CR11","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1002\/bbb.159","volume":"3","author":"PT Pienkos","year":"2009","unstructured":"Pienkos PT, Darzins AL (2009) The promise and challenges of microalgal-derived biofuels. Biofuels Bioprod Biorefin 3:431\u2013440. https:\/\/doi.org\/10.1002\/bbb.159","journal-title":"Biofuels Bioprod Biorefin"},{"key":"29_CR12","doi-asserted-by":"publisher","first-page":"1239","DOI":"10.1016\/j.rser.2015.04.131","volume":"50","author":"G De Bhowmick","year":"2015","unstructured":"De Bhowmick G, Koduru L, Sen R (2015) Metabolic pathway engineering towards enhancing microalgal lipid biosynthesis for biofuel application\u2014a review. Renew Sustain Energy Rev 50:1239\u20131253. https:\/\/doi.org\/10.1016\/j.rser.2015.04.131","journal-title":"Renew Sustain Energy Rev"},{"key":"29_CR13","doi-asserted-by":"publisher","first-page":"1448","DOI":"10.1016\/j.biotechadv.2014.09.002","volume":"32","author":"S-H Ho","year":"2014","unstructured":"Ho S-H, Ye X, Hasunuma T et al (2014) Perspectives on engineering strategies for improving biofuel production from microalgae\u2014a critical review. Biotechnol Adv 32:1448\u20131459. https:\/\/doi.org\/10.1016\/j.biotechadv.2014.09.002","journal-title":"Biotechnol Adv"},{"key":"29_CR14","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1186\/s13068-024-02461-0","volume":"17","author":"M Wang","year":"2024","unstructured":"Wang M, Ye X, Bi H et al (2024) Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities. Biotechnol Biofuels Bioprod 17:10. https:\/\/doi.org\/10.1186\/s13068-024-02461-0","journal-title":"Biotechnol Biofuels Bioprod"},{"key":"29_CR15","doi-asserted-by":"publisher","first-page":"775","DOI":"10.1016\/j.copbio.2011.04.024","volume":"22","author":"F Zhang","year":"2011","unstructured":"Zhang F, Rodriguez S, Keasling JD (2011) Metabolic engineering of microbial pathways for advanced biofuels production. Curr Opin Biotechnol 22:775\u2013783. https:\/\/doi.org\/10.1016\/j.copbio.2011.04.024","journal-title":"Curr Opin Biotechnol"},{"key":"29_CR16","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/j.ymben.2015.06.008","volume":"31","author":"D Liu","year":"2015","unstructured":"Liu D, Evans T, Zhang F (2015) Applications and advances of metabolite biosensors for metabolic engineering. Metab Eng 31:35\u201343. https:\/\/doi.org\/10.1016\/j.ymben.2015.06.008","journal-title":"Metab Eng"},{"key":"29_CR17","doi-asserted-by":"publisher","first-page":"3863","DOI":"10.1016\/j.rser.2017.10.085","volume":"82","author":"P Majidian","year":"2018","unstructured":"Majidian P, Tabatabaei M, Zeinolabedini M et al (2018) Metabolic engineering of microorganisms for biofuel production. Renew Sustain Energy Rev 82:3863\u20133885. https:\/\/doi.org\/10.1016\/j.rser.2017.10.085","journal-title":"Renew Sustain Energy Rev"},{"key":"29_CR18","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13068-018-1181-1","volume":"11","author":"S Jagadevan","year":"2018","unstructured":"Jagadevan S, Banerjee A, Banerjee C et al (2018) Recent developments in synthetic biology and metabolic engineering in microalgae towards biofuel production. Biotechnol Biofuels 11:1\u201321. https:\/\/doi.org\/10.1186\/s13068-018-1181-1","journal-title":"Biotechnol Biofuels"},{"key":"29_CR19","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1016\/j.biortech.2010.06.014","volume":"102","author":"JAV Costa","year":"2011","unstructured":"Costa JAV, De Morais MG (2011) The role of biochemical engineering in the production of biofuels from microalgae. Bioresour Technol 102:2\u20139. https:\/\/doi.org\/10.1016\/j.biortech.2010.06.014","journal-title":"Bioresour Technol"},{"key":"29_CR20","doi-asserted-by":"publisher","unstructured":"Zorrilla L\u00f3pez U, Masip Vil\u00e0 G, Arj\u00f3 Pont Get al (2013) Engineering metabolic pathways in plants by multigene transformation. https:\/\/doi.org\/10.1387\/ijdb.130162pc","DOI":"10.1387\/ijdb.130162pc"},{"key":"29_CR21","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1016\/j.biotechadv.2007.02.001","volume":"25","author":"Y Chisti","year":"2007","unstructured":"Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:294\u2013306. https:\/\/doi.org\/10.1016\/j.biotechadv.2007.02.001","journal-title":"Biotechnol Adv"},{"key":"29_CR22","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/j.rser.2017.01.070","volume":"73","author":"N Gaurav","year":"2017","unstructured":"Gaurav N, Sivasankari S, Kiran GS et al (2017) Utilization of bioresources for sustainable biofuels: a review. Renew Sustain Energy Rev 73:205\u2013214. https:\/\/doi.org\/10.1016\/j.rser.2017.01.070","journal-title":"Renew Sustain Energy Rev"},{"key":"29_CR23","doi-asserted-by":"publisher","first-page":"101071","DOI":"10.1016\/j.pecs.2023.101071","volume":"96","author":"KS Khoo","year":"2023","unstructured":"Khoo KS, Ahmad I, Chew KW et al (2023) Enhanced microalgal lipid production for biofuel using different strategies including genetic modification of microalgae: a review. Prog Energy Combust Sci 96:101071. https:\/\/doi.org\/10.1016\/j.pecs.2023.101071","journal-title":"Prog Energy Combust Sci"},{"key":"29_CR24","doi-asserted-by":"publisher","first-page":"1476","DOI":"10.1016\/j.biotechadv.2014.10.003","volume":"32","author":"S Bellou","year":"2014","unstructured":"Bellou S, Baeshen MN, Elazzazy AM et al (2014) Microalgal lipids biochemistry and biotechnological perspectives. Biotechnol Adv 32:1476\u20131493. https:\/\/doi.org\/10.1016\/j.biotechadv.2014.10.003","journal-title":"Biotechnol Adv"},{"key":"29_CR25","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1475-2859-10-91","volume":"10","author":"W-L Yu","year":"2011","unstructured":"Yu W-L, Ansari W, Schoepp NG et al (2011) Modifications of the metabolic pathways of lipid and triacylglycerol production in microalgae. Microb Cell Fact 10:1\u201311. https:\/\/doi.org\/10.1186\/1475-2859-10-91","journal-title":"Microb Cell Fact"},{"key":"29_CR26","doi-asserted-by":"publisher","first-page":"486","DOI":"10.1128\/ec.00364-09","volume":"9","author":"R Radakovits","year":"2010","unstructured":"Radakovits R, Jinkerson RE, Darzins A et al(2010) Genetic engineering of algae for enhanced biofuel production. Eukaryot Cell 9:486\u2013501. https:\/\/doi.org\/10.1128\/ec.00364-09","journal-title":"Eukaryot Cell"},{"key":"29_CR27","doi-asserted-by":"publisher","first-page":"9083","DOI":"10.3390\/su12219083","volume":"12","author":"Z Shokravi","year":"2020","unstructured":"Shokravi Z, Shokravi H, Chyuan OH et al (2020) Improving \u2018lipid productivity\u2019 in microalgae by bilateral enhancement of biomass and lipid contents: a review. Sustainability 12:9083. https:\/\/doi.org\/10.3390\/su12219083","journal-title":"Sustainability"},{"key":"29_CR28","doi-asserted-by":"publisher","first-page":"1858","DOI":"10.1016\/S0021-9258(18)52372-3","volume":"266","author":"D Post-Beittenmiller","year":"1991","unstructured":"Post-Beittenmiller D, Jaworski JG, Ohlrogge JB(1991) In vivo pools of free and acylated acyl carrier proteins in spinach. J Biol Chem 266:1858\u20131865. https:\/\/doi.org\/10.1016\/S0021-9258(18)52372-3","journal-title":"J Biol Chem"},{"key":"29_CR29","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1104\/pp.100.2.923","volume":"100","author":"D Post-Beittenmiller","year":"1992","unstructured":"Post-Beittenmiller D, Roughan G, Ohlrogge JB(1992) Regulation of plant fatty acid biosynthesis: analysis of acyl-coenzyme A and acyl-acyl carrier protein substrate pools in spinach and pea chloroplasts. Plant Physiol 100:923\u2013930. https:\/\/doi.org\/10.1104\/pp.100.2.923","journal-title":"Plant Physiol"},{"key":"29_CR30","doi-asserted-by":"publisher","first-page":"100054","DOI":"10.1016\/j.nexus.2022.100054","volume":"5","author":"A Mulgund","year":"2022","unstructured":"Mulgund A (2022) Increasing lipid accumulation in microalgae through environmental manipulation, metabolic and genetic engineering: a review in the energy NEXUS framework. Energy Nexus 5:100054. https:\/\/doi.org\/10.1016\/j.nexus.2022.100054","journal-title":"Energy Nexus"},{"key":"29_CR31","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1016\/j.algal.2016.03.007","volume":"16","author":"W-C Chang","year":"2016","unstructured":"Chang W-C, Zheng H-Q, Chen C-NN (2016) Comparative transcriptome analysis reveals a potential photosynthate partitioning mechanism between lipid and starch biosynthetic pathways in green microalgae. Algal Res 16:54\u201362. https:\/\/doi.org\/10.1016\/j.algal.2016.03.007","journal-title":"Algal Res"},{"key":"29_CR32","doi-asserted-by":"publisher","first-page":"395","DOI":"10.1016\/j.plipres.2013.05.002","volume":"52","author":"M-H Liang","year":"2013","unstructured":"Liang M-H, Jiang J-G (2013) Advancing oleaginous microorganisms to produce lipid via metabolic engineering technology. Prog Lipid Res 52:395\u2013408. https:\/\/doi.org\/10.1016\/j.plipres.2013.05.002","journal-title":"Prog Lipid Res"},{"key":"29_CR33","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1007\/BF02941703","volume":"57","author":"TG Dunahay","year":"1996","unstructured":"Dunahay TG, Jarvis EE, Dais SS et al (1996) Manipulation of microalgal lipid production using genetic engineering. Appl Biochem Biotechnol 57:223\u2013231. https:\/\/doi.org\/10.1007\/BF02941703","journal-title":"Appl Biochem Biotechnol"},{"key":"29_CR34","first-page":"1","volume":"328","author":"J Sheehan","year":"1998","unstructured":"Sheehan J, Dunahay T, Benemann J et al (1998) A look back at the US Department of Energy\u2019s aquatic species program: biodiesel from algae. Natl Renew Energy Lab 328:1\u2013294.NREL\/TP-580-24190","journal-title":"Natl Renew Energy Lab"},{"key":"29_CR35","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1007\/s12088-015-0546-4","volume":"55","author":"AE Gomma","year":"2015","unstructured":"Gomma AE, Lee S-K, Sun SM et al (2015) Improvement in oil production by increasing malonyl-CoA and glycerol-3-phosphate pools in Scenedesmus quadricauda. Indian J Microbiol 55:447\u2013455. https:\/\/doi.org\/10.1007\/s12088-015-0546-4","journal-title":"Indian J Microbiol"},{"key":"29_CR36","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1002\/btpr.716","volume":"28","author":"X Zhang","year":"2012","unstructured":"Zhang X, Agrawal A, San K (2012) Improving fatty acid production in Escherichia coli through the overexpression of malonyl coA-acyl carrier protein transacylase. Biotechnol Prog 28:60\u201365. https:\/\/doi.org\/10.1002\/btpr.716","journal-title":"Biotechnol Prog"},{"key":"29_CR37","doi-asserted-by":"publisher","first-page":"620","DOI":"10.1002\/bab.1531","volume":"64","author":"J Chen","year":"2017","unstructured":"Chen J, Liu W, Hu D et al (2017) Identification of a malonyl CoA-acyl carrier protein transacylase and its regulatory role in fatty acid biosynthesis in oleaginous microalga Nannochloropsis oceanica. Biotechnol Appl Biochem 64:620\u2013626. https:\/\/doi.org\/10.1002\/bab.1531","journal-title":"Biotechnol Appl Biochem"},{"key":"29_CR38","doi-asserted-by":"publisher","first-page":"5382","DOI":"10.1021\/acs.jafc.8b01026","volume":"66","author":"Z Li","year":"2018","unstructured":"Li Z, Meng T, Ling X et al (2018) Overexpression of malonyl-CoA: ACP transacylase in Schizochytrium sp. to improve polyunsaturated fatty acid production. J Agric Food Chem 66:5382\u20135391. https:\/\/doi.org\/10.1021\/acs.jafc.8b01026","journal-title":"J Agric Food Chem"},{"key":"29_CR39","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1016\/j.algal.2018.02.017","volume":"31","author":"Y Fan","year":"2018","unstructured":"Fan Y, Yuan C, Jin Y et al (2018) Characterization of 3-ketoacyl-CoA synthase in a nervonic acid producing oleaginous microalgae Mychonastes afer. Algal Res 31:225\u2013231. https:\/\/doi.org\/10.1016\/j.algal.2018.02.017","journal-title":"Algal Res"},{"key":"29_CR40","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13068-017-0786-0","volume":"10","author":"S Balamurugan","year":"2017","unstructured":"Balamurugan S, Wang X, Wang H-L et al (2017) Occurrence of plastidial triacylglycerol synthesis and the potential regulatory role of AGPAT in the model diatom Phaeodactylum tricornutum. Biotechnol Biofuels 10:1\u201314. https:\/\/doi.org\/10.1186\/s13068-017-0786-0","journal-title":"Biotechnol Biofuels"},{"key":"29_CR41","doi-asserted-by":"publisher","first-page":"2158","DOI":"10.1111\/pbi.12572","volume":"14","author":"Y Yamaoka","year":"2016","unstructured":"Yamaoka Y, Achard D, Jang S et al (2016) Identification of a Chlamydomonas plastidial 2-lysophosphatidic acid acyltransferase and its use to engineer microalgae with increased oil content. Plant Biotechnol J 14:2158\u20132167. https:\/\/doi.org\/10.1111\/pbi.12572","journal-title":"Plant Biotechnol J"},{"key":"29_CR42","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12934-018-0906-y","volume":"17","author":"L-G Zou","year":"2018","unstructured":"Zou L-G, Chen J-W, Zheng D-L et al (2018) High-efficiency promoter-driven coordinated regulation of multiple metabolic nodes elevates lipid accumulation in the model microalga Phaeodactylum tricornutum. Microb Cell Fact 17:1\u20138. https:\/\/doi.org\/10.1186\/s12934-018-0906-y","journal-title":"Microb Cell Fact"},{"key":"29_CR43","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13068-016-0478-1","volume":"9","author":"Y-F Niu","year":"2016","unstructured":"Niu Y-F, Wang X, Hu D-X et al (2016) Molecular characterization of a glycerol-3-phosphate acyltransferase reveals key features essential for triacylglycerol production in Phaeodactylum tricornutum. Biotechnol Biofuels 9:1\u201311. https:\/\/doi.org\/10.1186\/s13068-016-0478-1","journal-title":"Biotechnol Biofuels"},{"key":"29_CR44","doi-asserted-by":"publisher","first-page":"907","DOI":"10.1007\/s10811-015-0634-1","volume":"28","author":"U Iskandarov","year":"2016","unstructured":"Iskandarov U, Sitnik S, Shtaida N et al (2016) Cloning and characterization of a GPAT-like gene from the microalga Lobosphaera incisa (Trebouxiophyceae): overexpression in Chlamydomonas reinhardtii enhances TAG production. J Appl Phycol 28:907\u2013919. https:\/\/doi.org\/10.1007\/s10811-015-0634-1","journal-title":"J Appl Phycol"},{"key":"29_CR45","doi-asserted-by":"publisher","first-page":"4558","DOI":"10.3390\/md11114558","volume":"11","author":"Y-F Niu","year":"2013","unstructured":"Niu Y-F, Zhang M-H, Li D-W et al (2013) Improvement of neutral lipid and polyunsaturated fatty acid biosynthesis by overexpressing a type 2 diacylglycerol acyltransferase in marine diatom Phaeodactylum tricornutum. Mar Drugs 11:4558\u20134569. https:\/\/doi.org\/10.3390\/md11114558","journal-title":"Mar Drugs"},{"key":"29_CR46","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13068-017-0858-1","volume":"10","author":"H Wei","year":"2017","unstructured":"Wei H, Shi Y, Ma X et al (2017) A type-I diacylglycerol acyltransferase modulates triacylglycerol biosynthesis and fatty acid composition in the oleaginous microalga Nannochloropsis oceanica. Biotechnol Biofuels 10:1\u201318. https:\/\/doi.org\/10.1186\/s13068-017-0858-1","journal-title":"Biotechnol Biofuels"},{"key":"29_CR47","doi-asserted-by":"publisher","first-page":"2364","DOI":"10.1016\/j.febslet.2013.06.002","volume":"587","author":"C-H Hung","year":"2013","unstructured":"Hung C-H, Ho M-Y, Kanehara K, Nakamura Y (2013) Functional study of diacylglycerol acyltransferase type 2 family in Chlamydomonas reinhardtii. FEBS Lett 587:2364\u20132370. https:\/\/doi.org\/10.1016\/j.febslet.2013.06.002","journal-title":"FEBS Lett"},{"key":"29_CR48","doi-asserted-by":"publisher","first-page":"808","DOI":"10.1111\/pbi.12210","volume":"12","author":"M Iwai","year":"2014","unstructured":"Iwai M, Ikeda K, Shimojima M, Ohta H (2014) Enhancement of extraplastidic oil synthesis in Chlamydomonas reinhardtii using a type-2 diacylglycerol acyltransferase with a phosphorus starvation\u2013inducible promoter. Plant Biotechnol J 12:808\u2013819. https:\/\/doi.org\/10.1111\/pbi.12210","journal-title":"Plant Biotechnol J"},{"key":"29_CR49","doi-asserted-by":"publisher","first-page":"336","DOI":"10.1002\/biot.201500272","volume":"11","author":"C Chen","year":"2016","unstructured":"Chen C, Kao A, Tsai Z et al (2016) Expression of type 2 diacylglycerol acyltransferase gene DGTT1 from enhances lipid production in Scenedesmus obliquus. Biotechnol J 11:336\u2013344. https:\/\/doi.org\/10.1002\/biot.201500272","journal-title":"Biotechnol J"},{"key":"29_CR50","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13068-016-0686-8","volume":"10","author":"K Zienkiewicz","year":"2017","unstructured":"Zienkiewicz K, Zienkiewicz A, Poliner Eet al (2017) Nannochloropsis, a rich source of diacylglycerol acyltransferases for engineering of triacylglycerol content in different hosts. Biotechnol Biofuels 10:1\u201320. https:\/\/doi.org\/10.1186\/s13068-016-0686-8","journal-title":"Biotechnol Biofuels"},{"key":"29_CR51","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/j.jbiotec.2016.05.005","volume":"229","author":"D-W Li","year":"2016","unstructured":"Li D-W, Cen S-Y, Liu Y-H et al (2016) A type 2 diacylglycerol acyltransferase accelerates the triacylglycerol biosynthesis in heterokont oleaginous microalga Nannochloropsis oceanica. J Biotechnol 229:65\u201371. https:\/\/doi.org\/10.1016\/j.jbiotec.2016.05.005","journal-title":"J Biotechnol"},{"key":"29_CR52","doi-asserted-by":"publisher","first-page":"526","DOI":"10.1007\/s12275-012-2041-5","volume":"50","author":"H-J Hsieh","year":"2012","unstructured":"Hsieh H-J, Su C-H, Chien L-J (2012) Accumulation of lipid production in Chlorella minutissima by triacylglycerol biosynthesis-related genes cloned from Saccharomyces cerevisiae and Yarrowia lipolytica. J Microbiol 50:526\u2013534. https:\/\/doi.org\/10.1007\/s12275-012-2041-5","journal-title":"J Microbiol"},{"key":"29_CR53","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/j.algal.2016.01.005","volume":"14","author":"TA Beacham","year":"2016","unstructured":"Beacham TA, Ali ST (2016) Growth dependent silencing and resetting of DGA1 transgene in Nannochloropsis salina. Algal Res 14:65\u201371. https:\/\/doi.org\/10.1016\/j.algal.2016.01.005","journal-title":"Algal Res"},{"key":"29_CR54","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.jbiotec.2012.04.006","volume":"162","author":"M La Russa","year":"2012","unstructured":"La Russa M, Bogen C, Uhmeyer A et al (2012) Functional analysis of three type-2 DGAT homologue genes for triacylglycerol production in the green microalga Chlamydomonas reinhardtii. J Biotechnol 162:13\u201320. https:\/\/doi.org\/10.1016\/j.jbiotec.2012.04.006","journal-title":"J Biotechnol"},{"key":"29_CR55","doi-asserted-by":"publisher","first-page":"945","DOI":"10.1093\/mp\/sss040","volume":"5","author":"X-D Deng","year":"2012","unstructured":"Deng X-D, Gu B, Li Y-J et al (2012) The roles of acyl-CoA: diacylglycerol acyltransferase 2 genes in the biosynthesis of triacylglycerols by the green algae Chlamydomonas reinhardtii. Mol Plant 5:945\u2013947. https:\/\/doi.org\/10.1093\/mp\/sss040","journal-title":"Mol Plant"},{"key":"29_CR56","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12934-014-0100-9","volume":"13","author":"Y-H Ma","year":"2014","unstructured":"Ma Y-H, Wang X, Niu Y-F et al (2014) Antisense knockdown of pyruvate dehydrogenase kinase promotes the neutral lipid accumulation in the diatom Phaeodactylum tricornutum. Microb Cell Fact 13:1\u20139. https:\/\/doi.org\/10.1186\/s12934-014-0100-9","journal-title":"Microb Cell Fact"},{"key":"29_CR57","doi-asserted-by":"publisher","first-page":"19748","DOI":"10.1073\/pnas.1309299110","volume":"110","author":"EM Trentacoste","year":"2013","unstructured":"Trentacoste EM, Shrestha RP, Smith SR et al (2013) Metabolic engineering of lipid catabolism increases microalgal lipid accumulation without compromising growth. Proc Natl Acad Sci 110:19748\u201319753. https:\/\/doi.org\/10.1073\/pnas.1309299110","journal-title":"Proc Natl Acad Sci"},{"key":"29_CR58","doi-asserted-by":"publisher","first-page":"1103","DOI":"10.1104\/pp.125.2.1103","volume":"125","author":"K Dehesh","year":"2001","unstructured":"Dehesh K, Tai H, Edwards P et al (2001) Overexpression of 3-ketoacyl-acyl-carrier protein synthase IIIs in plants reduces the rate of lipid synthesis. Plant Physiol 125:1103\u20131114. https:\/\/doi.org\/10.1104\/pp.125.2.1103","journal-title":"Plant Physiol"},{"key":"29_CR59","doi-asserted-by":"publisher","first-page":"552","DOI":"10.1016\/j.bbalip.2018.10.004","volume":"1864","author":"X-M Sun","year":"2019","unstructured":"Sun X-M, Ren L-J, Zhao Q-Y et al (2019) Enhancement of lipid accumulation in microalgae by metabolic engineering. Biochim Biophys Acta Mol Cell Biol Lipids 1864:552\u2013566. https:\/\/doi.org\/10.1016\/j.bbalip.2018.10.004","journal-title":"Biochim Biophys Acta Mol Cell Biol Lipids"},{"key":"29_CR60","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1016\/j.tibtech.2014.07.004","volume":"32","author":"AJ Klok","year":"2014","unstructured":"Klok AJ, Lamers PP, Martens DE et al (2014) Edible oils from microalgae: insights in TAG accumulation. Trends Biotechnol 32:521\u2013528. https:\/\/doi.org\/10.1016\/j.tibtech.2014.07.004","journal-title":"Trends Biotechnol"},{"key":"29_CR61","doi-asserted-by":"publisher","first-page":"1971","DOI":"10.1194\/jlr.M006304","volume":"51","author":"D Shan","year":"2010","unstructured":"Shan D, Li J, Wu L et al (2010) GPAT3 and GPAT4 are regulated by insulin-stimulated phosphorylation and play distinct roles in adipogenesis. J Lipid Res 51:1971\u20131981. https:\/\/doi.org\/10.1194\/jlr.M006304","journal-title":"J Lipid Res"},{"key":"29_CR62","doi-asserted-by":"publisher","first-page":"8877","DOI":"10.1074\/jbc.M111646200","volume":"277","author":"P Oelkers","year":"2002","unstructured":"Oelkers P, Cromley D, Padamsee M et al (2002) The DGA1 gene determines a second triglyceride synthetic pathway in yeast. J Biol Chem 277:8877\u20138881. https:\/\/doi.org\/10.1074\/jbc.M111646200","journal-title":"J Biol Chem"},{"key":"29_CR63","doi-asserted-by":"publisher","first-page":"13018","DOI":"10.1073\/pnas.95.22.13018","volume":"95","author":"S Cases","year":"1998","unstructured":"Cases S, Smith SJ, Zheng Y-W et al (1998) Identification of a gene encoding an acyl CoA: diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis. Proc Natl Acad Sci 95:13018\u201313023. https:\/\/doi.org\/10.1073\/pnas.95.22.13018","journal-title":"Proc Natl Acad Sci"},{"key":"29_CR64","doi-asserted-by":"publisher","first-page":"1645","DOI":"10.1105\/tpc.113.121418","volume":"26","author":"J Li","year":"2014","unstructured":"Li J, Han D, Wang D et al (2014) Choreography of transcriptomes and lipidomes of Nannochloropsis reveals the mechanisms of oil synthesis in microalgae. Plant Cell 26:1645\u20131665. https:\/\/doi.org\/10.1105\/tpc.113.121418","journal-title":"Plant Cell"},{"key":"29_CR65","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1752-0509-3-4","volume":"3","author":"NR Boyle","year":"2009","unstructured":"Boyle NR, Morgan JA (2009) Flux balance analysis of primary metabolism in Chlamydomonas reinhardtii. BMC Syst Biol 3:1\u201314. https:\/\/doi.org\/10.1186\/1752-0509-3-4","journal-title":"BMC Syst Biol"},{"key":"29_CR66","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ymben.2014.10.006","volume":"27","author":"J Xue","year":"2015","unstructured":"Xue J, Niu Y-F, Huang T et al (2015) Genetic improvement of the microalga Phaeodactylum tricornutum for boosting neutral lipid accumulation. Metab Eng 27:1\u20139. https:\/\/doi.org\/10.1016\/j.ymben.2014.10.006","journal-title":"Metab Eng"},{"key":"29_CR67","doi-asserted-by":"publisher","first-page":"12772","DOI":"10.1016\/j.ijhydene.2016.05.115","volume":"41","author":"E Eroglu","year":"2016","unstructured":"Eroglu E, Melis A (2016) Microalgal hydrogen production research. Int J Hydrogen Energy 41:12772\u201312798. https:\/\/doi.org\/10.1016\/j.ijhydene.2016.05.115","journal-title":"Int J Hydrogen Energy"},{"key":"29_CR68","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.bej.2003.10.006","volume":"19","author":"Y Guan","year":"2004","unstructured":"Guan Y, Deng M, Yu X et al (2004) Two-stage photo-biological production of hydrogen by marine green alga Platymonas subcordiformis. Biochem Eng J 19:69\u201373. https:\/\/doi.org\/10.1016\/j.bej.2003.10.006","journal-title":"Biochem Eng J"},{"key":"29_CR69","doi-asserted-by":"publisher","first-page":"1563","DOI":"10.1016\/j.ijhydene.2006.06.038","volume":"31","author":"A Melis","year":"2006","unstructured":"Melis A, Melnicki MR (2006) Integrated biological hydrogen production. Int J Hydrogen Energy 31:1563\u20131573. https:\/\/doi.org\/10.1016\/j.ijhydene.2006.06.038","journal-title":"Int J Hydrogen Energy"},{"key":"29_CR70","doi-asserted-by":"publisher","unstructured":"Johanningmeier U, Fischer D (2010) Perspective for the use of genetic transformants in order to enhance the synthesis of the desired metabolites: engineering chloroplasts of microalgae for the production of bioactive compounds. Bio-Farms Nutraceuticals Funct Food Saf Contr Biosens 2010:144\u2013151. https:\/\/doi.org\/10.1007\/978-1-4419-7347-4_11","DOI":"10.1007\/978-1-4419-7347-4_11"},{"key":"29_CR71","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1016\/j.cbpa.2013.03.038","volume":"17","author":"JA Gimpel","year":"2013","unstructured":"Gimpel JA, Specht EA, Georgianna DRet al (2013) Advances in microalgae engineering and synthetic biology applications for biofuel production. Curr Opin Chem Biol 17:489\u2013495. https:\/\/doi.org\/10.1016\/j.cbpa.2013.03.038","journal-title":"Curr Opin Chem Biol"},{"key":"29_CR72","doi-asserted-by":"publisher","first-page":"743","DOI":"10.1016\/j.rser.2017.08.042","volume":"81","author":"ES Shuba","year":"2018","unstructured":"Shuba ES, Kifle D (2018) Microalgae to biofuels: \u2018Promising\u2019 alternative and renewable energy, review. Renew Sustain Energy Rev 81:743\u2013755. https:\/\/doi.org\/10.1016\/j.rser.2017.08.042","journal-title":"Renew Sustain Energy Rev"},{"key":"29_CR73","doi-asserted-by":"publisher","first-page":"1194","DOI":"10.1016\/j.biortech.2017.07.085","volume":"243","author":"W Khetkorn","year":"2017","unstructured":"Khetkorn W, Rastogi RP, Incharoensakdi A et al (2017) Microalgal hydrogen production\u2013a review. Bioresour Technol 243:1194\u20131206. https:\/\/doi.org\/10.1016\/j.biortech.2017.07.085","journal-title":"Bioresour Technol"},{"key":"29_CR74","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1016\/j.copbio.2009.06.002","volume":"20","author":"LL Beer","year":"2009","unstructured":"Beer LL, Boyd ES, Peters JW, Posewitz MC (2009) Engineering algae for biohydrogen and biofuel production. Curr Opin Biotechnol 20:264\u2013271. https:\/\/doi.org\/10.1016\/j.copbio.2009.06.002","journal-title":"Curr Opin Biotechnol"},{"key":"29_CR75","doi-asserted-by":"publisher","first-page":"17548","DOI":"10.1073\/pnas.0704205104","volume":"104","author":"R Surzycki","year":"2007","unstructured":"Surzycki R, Cournac L, Peltier G et al (2007) Potential for hydrogen production with inducible chloroplast gene expression in Chlamydomonas. Proc Natl Acad Sci 104:17548\u201317553. https:\/\/doi.org\/10.1073\/pnas.0704205104","journal-title":"Proc Natl Acad Sci"},{"key":"29_CR76","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1016\/j.tibtech.2011.06.008","volume":"29","author":"MG Esqu\u00edvel","year":"2011","unstructured":"Esqu\u00edvel MG, Amaro HM, Pinto TS et al(2011) Efficient H2 production via Chlamydomonas reinhardtii. Trends Biotechnol 29:595\u2013600. https:\/\/doi.org\/10.1016\/j.tibtech.2011.06.008","journal-title":"Trends Biotechnol"},{"key":"29_CR77","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1007\/s11120-014-9991-x","volume":"123","author":"A Dubini","year":"2015","unstructured":"Dubini A, Ghirardi ML (2015) Engineering photosynthetic organisms for the production of biohydrogen. Photosynth Res 123:241\u2013253. https:\/\/doi.org\/10.1007\/s11120-014-9991-x","journal-title":"Photosynth Res"},{"key":"29_CR78","doi-asserted-by":"publisher","first-page":"262","DOI":"10.1016\/j.tibtech.2010.01.007","volume":"28","author":"H-S Lee","year":"2010","unstructured":"Kruse O, Rupprecht J, Bader KP, Thomas-Hall S, Schenk PM, Finazzi G, Hankamer B (2005) Improved photobiological H2 production in engineered green algal cells. J Biol Chem, 280(40), 34170\u201334177. https:\/\/doi.org\/10.1074\/jbc.M503840200","journal-title":"Trends Biotechnol"},{"key":"29_CR79","doi-asserted-by":"publisher","unstructured":"Zhang J, Xue D, Wang C et al (2023) Genetic engineering for biohydrogen production from microalgae. iScience 26. https:\/\/doi.org\/10.1016\/j.isci.2023.107255","DOI":"10.1016\/j.isci.2023.107255"},{"key":"29_CR80","doi-asserted-by":"publisher","first-page":"1373","DOI":"10.1007\/s10529-010-0326-5","volume":"32","author":"E Specht","year":"2010","unstructured":"Specht E, Miyake-Stoner S, Mayfield S (2010) Micro-algae come of age as a platform for recombinant protein production. Biotechnol Lett 32:1373\u20131383. https:\/\/doi.org\/10.1007\/s10529-010-0326-5","journal-title":"Biotechnol Lett"},{"key":"29_CR81","doi-asserted-by":"publisher","first-page":"3425","DOI":"10.3390\/md11093425","volume":"11","author":"P Heydarizadeh","year":"2013","unstructured":"Heydarizadeh P, Poirier I, Loizeau D x(2013) Plastids of marine phytoplankton produce bioactive pigments and lipids. Mar Drugs 11:3425\u20133471. https:\/\/doi.org\/10.3390\/md11093425","journal-title":"Mar Drugs"},{"key":"29_CR82","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1007\/s00294-011-0339-1","volume":"57","author":"M Terashima","year":"2011","unstructured":"Terashima M, Specht M, Hippler M (2011) The chloroplast proteome: a survey from the Chlamydomonas reinhardtii perspective with a focus on distinctive features. Curr Genet 57:151\u2013168. https:\/\/doi.org\/10.1007\/s00294-011-0339-1","journal-title":"Curr Genet"},{"key":"29_CR83","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1007\/s11120-014-9994-7","volume":"123","author":"BA Rasala","year":"2015","unstructured":"Rasala BA, Mayfield SP (2015) Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses. Photosynth Res 123:227\u2013239. https:\/\/doi.org\/10.1007\/s11120-014-9994-7","journal-title":"Photosynth Res"},{"key":"29_CR84","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1007\/s12033-010-9348-4","volume":"48","author":"AT Coragliotti","year":"2011","unstructured":"Coragliotti AT, Beligni MV, Franklin SE, Mayfield SP (2011) Molecular factors affecting the accumulation of recombinant proteins in the Chlamydomonas reinhardtii chloroplast. Mol Biotechnol 48:60\u201375. https:\/\/doi.org\/10.1007\/s12033-010-9348-4","journal-title":"Mol Biotechnol"},{"key":"29_CR85","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1111\/j.1365-313X.2008.03767.x","volume":"58","author":"A Molnar","year":"2009","unstructured":"Molnar A, Bassett A, Thuenemann E et al(2009) Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii. Plant J 58:165\u2013174. https:\/\/doi.org\/10.1111\/j.1365-313X.2008.03767.x","journal-title":"Plant J"},{"key":"29_CR86","doi-asserted-by":"publisher","first-page":"1164","DOI":"10.1128\/ec.05106-11","volume":"10","author":"H Cerutti","year":"2011","unstructured":"Cerutti H, Ma X, Msanne J, Repas T (2011) RNA-mediated silencing in algae: biological roles and tools for analysis of gene function. Eukaryot Cell 10:1164\u20131172. https:\/\/doi.org\/10.1128\/ec.05106-11","journal-title":"Eukaryot Cell"},{"key":"29_CR87","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1134\/S1021443713040146","volume":"60","author":"S Purton","year":"2013","unstructured":"Purton S, Szaub JB, Wannathong T et al (2013) Genetic engineering of algal chloroplasts: progress and prospects. Russ J Plant Physiol 60:491\u2013499. https:\/\/doi.org\/10.1134\/S1021443713040146","journal-title":"Russ J Plant Physiol"},{"key":"29_CR88","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s13205-017-0831-5","volume":"7","author":"V Anand","year":"2017","unstructured":"Anand V, Singh PK, Banerjee C, Shukla P (2017) Proteomic approaches in microalgae: perspectives and applications. 3 Biotech 7:1\u201310. https:\/\/doi.org\/10.1007\/s13205-017-0831-5","journal-title":"3 Biotech"},{"key":"29_CR89","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1002\/biot.201500284","volume":"11","author":"C Banerjee","year":"2016","unstructured":"Banerjee C, Singh PK, Shukla P (2016) Microalgal bioengineering for sustainable energy development: recent transgenesis and metabolic engineering strategies. Biotechnol J 11:303\u2013314. https:\/\/doi.org\/10.1002\/biot.201500284","journal-title":"Biotechnol J"},{"key":"29_CR90","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13068-015-0277-0","volume":"8","author":"Y Kasai","year":"2015","unstructured":"Kasai Y, Oshima K, Ikeda F et al (2015) Construction of a self-cloning system in the unicellular green alga Pseudochoricystis ellipsoidea. Biotechnol Biofuels 8:1\u201312. https:\/\/doi.org\/10.1186\/s13068-015-0277-0","journal-title":"Biotechnol Biofuels"},{"key":"29_CR91","doi-asserted-by":"publisher","first-page":"27810","DOI":"10.1038\/srep27810","volume":"6","author":"SE Shin","year":"2016","unstructured":"Shin SE, Lim JM, Koh HG et al (2016) CRISPR\/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii. Sci Rep 6:27810. https:\/\/doi.org\/10.1038\/srep27810","journal-title":"Sci Rep"},{"key":"29_CR92","doi-asserted-by":"publisher","first-page":"873","DOI":"10.1111\/tpj.12066","volume":"73","author":"I Sizova","year":"2013","unstructured":"Sizova I, Greiner A, Awasthi M et al (2013) Nuclear gene targeting in Chlamydomonas using engineered zinc-finger nucleases. Plant J 73:873\u2013882. https:\/\/doi.org\/10.1111\/tpj.12066","journal-title":"Plant J"},{"key":"29_CR93","doi-asserted-by":"publisher","unstructured":"Arshad S, Qadir ML, Hussain N et al (2025) Advances in CRISPR\/Cas9 technology: shaping the future of photosynthetic microorganisms for biofuel production. Funct Plant Biol 52. https:\/\/doi.org\/10.1071\/FP24255","DOI":"10.1071\/FP24255"},{"key":"29_CR94","doi-asserted-by":"publisher","first-page":"1019","DOI":"10.1016\/j.tibtech.2020.12.010","volume":"39","author":"MIS Naduthodi","year":"2021","unstructured":"Naduthodi MIS, Claassens NJ, D\u2019Adamo S et al (2021) Synthetic biology approaches to enhance microalgal productivity. Trends Biotechnol 39:1019\u20131036. https:\/\/doi.org\/10.1016\/j.tibtech.2020.12.010","journal-title":"Trends Biotechnol"},{"key":"29_CR95","doi-asserted-by":"publisher","unstructured":"Stavridou E, Karapetsi L, Nteve GM et al (2024) Landscape of microalgae omics and metabolic engineering research for strain improvement: an overview. Aquaculture 740803. https:\/\/doi.org\/10.1016\/j.aquaculture.2024.740803","DOI":"10.1016\/j.aquaculture.2024.740803"},{"key":"29_CR96","doi-asserted-by":"publisher","unstructured":"Abdullah M, Ali Z, Yasin MT et al (2024) Advancements in sustainable production of biofuel by microalgae: recent insights and future directions. Environ Res 119902. https:\/\/doi.org\/10.1016\/j.envres.2024.119902","DOI":"10.1016\/j.envres.2024.119902"},{"key":"29_CR97","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1038\/msb4100073","volume":"2","author":"E Andrianantoandro","year":"2006","unstructured":"Andrianantoandro E, Basu S, Karig DK, Weiss R(2006) Synthetic biology: new engineering rules for an emerging discipline. Mol Syst Biol 2:28\u20132006. https:\/\/doi.org\/10.1038\/msb4100073","journal-title":"Mol Syst Biol"},{"key":"29_CR98","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1007\/s12257-015-0386-4","volume":"21","author":"KMI Bashir","year":"2016","unstructured":"Bashir KMI, Kim M-S, Stahl U, Cho M-G (2016) Microalgae engineering toolbox: selectable and screenable markers. Biotechnol Bioprocess Eng 21:224\u2013235. https:\/\/doi.org\/10.1007\/s12257-015-0386-4","journal-title":"Biotechnol Bioprocess Eng"},{"key":"29_CR99","doi-asserted-by":"publisher","first-page":"989","DOI":"10.1016\/j.biotechadv.2011.08.015","volume":"30","author":"Y-S Jang","year":"2012","unstructured":"Jang Y-S, Park JM, Choi S et al (2012) Engineering of microorganisms for the production of biofuels and perspectives based on systems metabolic engineering approaches. Biotechnol Adv 30:989\u20131000. https:\/\/doi.org\/10.1016\/j.biotechadv.2011.08.015","journal-title":"Biotechnol Adv"},{"key":"29_CR100","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1038\/nprot.2009.203","volume":"5","author":"I Thiele","year":"2010","unstructured":"Thiele I, Palsson B\u00d8 (2010) A protocol for generating a high-quality genome-scale metabolic reconstruction. Nat Protoc 5:93\u2013121. https:\/\/doi.org\/10.1038\/nprot.2009.203","journal-title":"Nat Protoc"}],"container-title":["Methods in Molecular Biology","Biofuels from Algae"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5031-8_29","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T23:15:12Z","timestamp":1777590912000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5031-8_29"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071650301","9781071650318"],"references-count":100,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5031-8_29","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"1 April 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}