{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T17:11:42Z","timestamp":1779210702927,"version":"3.51.4"},"publisher-location":"New York, NY","reference-count":39,"publisher":"Springer US","isbn-type":[{"value":"9781071650530","type":"print"},{"value":"9781071650547","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5054-7_6","type":"book-chapter","created":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T16:55:11Z","timestamp":1779209711000},"page":"117-132","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Purification of Bacterial Lipopolysaccharide, and How to Study Its Interactions with Proteins"],"prefix":"10.1007","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7950-6781","authenticated-orcid":false,"given":"Athanasios","family":"Saragliadis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6945-8675","authenticated-orcid":false,"given":"Daniel","family":"Hatlem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3150-6752","authenticated-orcid":false,"given":"Dirk","family":"Linke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,5,20]]},"reference":[{"key":"6_CR1","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1002\/pro.4043","volume":"30","author":"J Asami","year":"2021","unstructured":"Asami J, Shimizu T (2021) Structural and functional understanding of the toll-like receptors. Protein Sci 30:761\u2013772","journal-title":"Protein Sci"},{"key":"6_CR2","doi-asserted-by":"publisher","first-page":"464","DOI":"10.1016\/j.tim.2010.06.005","volume":"18","author":"IC Sutcliffe","year":"2010","unstructured":"Sutcliffe IC (2010) A phylum level perspective on bacterial cell envelope architecture. Trends Microbiol 18:464\u2013470","journal-title":"Trends Microbiol"},{"key":"6_CR3","doi-asserted-by":"publisher","first-page":"779","DOI":"10.1016\/j.mib.2013.09.007","volume":"16","author":"G Zhang","year":"2013","unstructured":"Zhang G, Meredith TC, Kahne D (2013) On the essentiality of lipopolysaccharide to Gram-negative bacteria. Curr Opin Microbiol 16:779\u2013785","journal-title":"Curr Opin Microbiol"},{"key":"6_CR4","doi-asserted-by":"crossref","unstructured":"Bertani B, Ruiz N (2018) Function and biogenesis of lipopolysaccharides. EcoSal Plus 8(1):10.1128\/ecosalplus.ESP-0001-2018","DOI":"10.1128\/ecosalplus.esp-0001-2018"},{"key":"6_CR5","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1146\/annurev.bi.59.070190.001021","volume":"59","author":"CR Raetz","year":"1990","unstructured":"Raetz CR (1990) Biochemistry of endotoxins. Annu Rev Biochem 59:129\u2013170","journal-title":"Annu Rev Biochem"},{"key":"6_CR6","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1146\/annurev.biochem.71.110601.135414","volume":"71","author":"CR Raetz","year":"2002","unstructured":"Raetz CR, Whitfield C (2002) Lipopolysaccharide endotoxins. Annu Rev Biochem 71:635\u2013700","journal-title":"Annu Rev Biochem"},{"key":"6_CR7","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1038\/s41579-019-0201-x","volume":"17","author":"BW Simpson","year":"2019","unstructured":"Simpson BW, Trent MS (2019) Pushing the envelope: LPS modifications and their consequences. Nat Rev Microbiol 17:403\u2013416","journal-title":"Nat Rev Microbiol"},{"key":"6_CR8","doi-asserted-by":"publisher","first-page":"e1005596","DOI":"10.1371\/journal.ppat.1005596","volume":"12","author":"S Ranf","year":"2016","unstructured":"Ranf S (2016) Immune sensing of lipopolysaccharide in plants and animals: same but different. PLoS Pathog 12:e1005596","journal-title":"PLoS Pathog"},{"key":"6_CR9","first-page":"2431","volume":"31","author":"H Inagawa","year":"2011","unstructured":"Inagawa H, Kohchi C, Soma G (2011) Oral administration of lipopolysaccharides for the prevention of various diseases: benefit and usefulness. Anticancer Res 31:2431\u20132436","journal-title":"Anticancer Res"},{"key":"6_CR10","doi-asserted-by":"publisher","first-page":"1371","DOI":"10.1111\/bcp.13052","volume":"82","author":"V Doyen","year":"2016","unstructured":"Doyen V, Pilcer G, Dinh PH et al (2016) Inflammation induced by inhaled lipopolysaccharide depends on particle size in healthy volunteers. Br J Clin Pharmacol 82:1371\u20131381","journal-title":"Br J Clin Pharmacol"},{"key":"6_CR11","doi-asserted-by":"publisher","first-page":"220146","DOI":"10.1098\/rsob.220146","volume":"12","author":"AE Gauthier","year":"2022","unstructured":"Gauthier AE, Rotjan RD, Kagan JC (2022) Lipopolysaccharide detection by the innate immune system may be an uncommon defence strategy used in nature. Open Biol 12:220146","journal-title":"Open Biol"},{"key":"6_CR12","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.coi.2016.10.005","volume":"44","author":"CV Rosadini","year":"2017","unstructured":"Rosadini CV, Kagan JC (2017) Early innate immune responses to bacterial LPS. Curr Opin Immunol 44:14\u201319","journal-title":"Curr Opin Immunol"},{"key":"6_CR13","doi-asserted-by":"publisher","first-page":"681","DOI":"10.1016\/j.it.2021.06.001","volume":"42","author":"R Karki","year":"2021","unstructured":"Karki R, Kanneganti TD (2021) The \u2018cytokine storm\u2019: molecular mechanisms and therapeutic prospects. Trends Immunol 42:681\u2013705","journal-title":"Trends Immunol"},{"key":"6_CR14","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1128\/CMR.8.2.268","volume":"8","author":"JC Hurley","year":"1995","unstructured":"Hurley JC (1995) Endotoxemia: methods of detection and clinical correlates. Clin Microbiol Rev 8:268\u2013292","journal-title":"Clin Microbiol Rev"},{"key":"6_CR15","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/j.biologicals.2005.04.002","volume":"33","author":"CY Park","year":"2005","unstructured":"Park CY, Jung SH, Bak JP et al (2005) Comparison of the rabbit pyrogen test and Limulus amoebocyte lysate (LAL) assay for endotoxin in hepatitis B vaccines and the effect of aluminum hydroxide. Biologicals 33:145\u2013151","journal-title":"Biologicals"},{"key":"6_CR16","doi-asserted-by":"publisher","first-page":"36768","DOI":"10.1074\/jbc.M110.169003","volume":"285","author":"D Andres","year":"2010","unstructured":"Andres D, Hanke C, Baxa U et al (2010) Tailspike interactions with lipopolysaccharide effect DNA ejection from phage P22 particles in vitro. J Biol Chem 285:36768\u201336775","journal-title":"J Biol Chem"},{"key":"6_CR17","doi-asserted-by":"publisher","first-page":"119507","DOI":"10.1016\/j.bbamcr.2023.119507","volume":"1870","author":"A Sabnis","year":"2023","unstructured":"Sabnis A, Edwards AM (2023) Lipopolysaccharide as an antibiotic target. Biochim Biophys Acta, Mol Cell Res 1870:119507","journal-title":"Biochim Biophys Acta, Mol Cell Res"},{"key":"6_CR18","doi-asserted-by":"publisher","first-page":"440","DOI":"10.1111\/mmi.12568","volume":"92","author":"CL Johnson","year":"2014","unstructured":"Johnson CL, Ridley H, Marchetti R et al (2014) The antibacterial toxin colicin N binds to the inner core of lipopolysaccharide and close to its translocator protein. Mol Microbiol 92:440\u2013452","journal-title":"Mol Microbiol"},{"key":"6_CR19","doi-asserted-by":"publisher","first-page":"696","DOI":"10.1016\/j.it.2017.05.001","volume":"38","author":"JC Kagan","year":"2017","unstructured":"Kagan JC (2017) Lipopolysaccharide detection across the kingdoms of life. Trends Immunol 38:696\u2013704","journal-title":"Trends Immunol"},{"key":"6_CR20","doi-asserted-by":"publisher","first-page":"859","DOI":"10.1096\/fasebj.14.7.859","volume":"14","author":"NS Tan","year":"2000","unstructured":"Tan NS, Ho B, Ding JL (2000) High-affinity LPS binding domain(s) in recombinant factor C of a horseshoe crab neutralizes LPS-induced lethality. FASEB J 14:859\u2013870","journal-title":"FASEB J"},{"key":"6_CR21","doi-asserted-by":"publisher","first-page":"2543","DOI":"10.1104\/pp.17.01637","volume":"176","author":"K Shang-Guan","year":"2018","unstructured":"Shang-Guan K, Wang M, Htwe N et al (2018) Lipopolysaccharides trigger two successive bursts of reactive oxygen species at distinct cellular locations. Plant Physiol 176:2543\u20132556","journal-title":"Plant Physiol"},{"key":"6_CR22","doi-asserted-by":"crossref","unstructured":"Stromberg LR, Mendez HM, Mukundan H (2017) Detection methods for lipopolysaccharides: past and present. In: Samie A (ed) Escherichia coli \u2013 recent advances on physiology, pathogenesis and biotechnological applications. IntechOpen, London. https:\/\/www.intechopen.com\/chapters\/55322","DOI":"10.5772\/intechopen.68311"},{"issue":"7","key":"6_CR23","doi-asserted-by":"publisher","first-page":"3927","DOI":"10.3390\/ijms25073927","volume":"25","author":"F Dardelle","year":"2024","unstructured":"Dardelle F, Phelip C, Darabi M et al (2024) Diversity, complexity, and specificity of bacterial lipopolysaccharide (LPS) structures impacting their detection and quantification. Int J Mol Sci 25(7):3927","journal-title":"Int J Mol Sci"},{"key":"6_CR24","doi-asserted-by":"publisher","first-page":"2169","DOI":"10.1016\/j.bpj.2011.03.019","volume":"100","author":"C Jeworrek","year":"2011","unstructured":"Jeworrek C, Evers F, Howe J et al (2011) Effects of specific versus nonspecific ionic interactions on the structure and lateral organization of lipopolysaccharides. Biophys J 100:2169\u20132177","journal-title":"Biophys J"},{"key":"6_CR25","doi-asserted-by":"publisher","first-page":"1125482","DOI":"10.3389\/fcimb.2023.1125482","volume":"13","author":"D Hatlem","year":"2023","unstructured":"Hatlem D, Christensen M, Broeker NK et al (2023) A trimeric coiled-coil motif binds bacterial lipopolysaccharides with picomolar affinity. Front Cell Infect Microbiol 13:1125482","journal-title":"Front Cell Infect Microbiol"},{"key":"6_CR26","doi-asserted-by":"publisher","first-page":"e71","DOI":"10.14440\/jbm.2017.172","volume":"4","author":"EM Obeng","year":"2017","unstructured":"Obeng EM, Dullah EC, Razak NSA et al (2017) Elucidating endotoxin-biomolecule interactions with FRET: extending the frontiers of their supramolecular complexation. J Biol Methods 4:e71","journal-title":"J Biol Methods"},{"key":"6_CR27","doi-asserted-by":"publisher","first-page":"251","DOI":"10.3109\/07388551.2016.1141393","volume":"37","author":"EC Dullah","year":"2017","unstructured":"Dullah EC, Ongkudon CM (2017) Current trends in endotoxin detection and analysis of endotoxin-protein interactions. Crit Rev Biotechnol 37:251\u2013261","journal-title":"Crit Rev Biotechnol"},{"key":"6_CR28","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1016\/j.ijmm.2007.04.001","volume":"297","author":"R Jerala","year":"2007","unstructured":"Jerala R (2007) Structural biology of the LPS\u00a0recognition. Int J Med Microbiol 297:353\u2013363","journal-title":"Int J Med Microbiol"},{"key":"6_CR29","doi-asserted-by":"publisher","first-page":"1446","DOI":"10.1128\/JB.180.6.1446-1453.1998","volume":"180","author":"AJ Baumler","year":"1998","unstructured":"Baumler AJ, Norris TL, Lasco T et al (1998) IroN, a novel outer membrane siderophore receptor characteristic of Salmonella enterica. J\u00a0Bacteriol 180:1446\u20131453","journal-title":"J Bacteriol"},{"key":"6_CR30","doi-asserted-by":"publisher","first-page":"2958","DOI":"10.1038\/s41598-019-39590-8","volume":"9","author":"EE Kulikov","year":"2019","unstructured":"Kulikov EE, Golomidova AK, Prokhorov NS et al (2019) High-throughput LPS profiling as a tool for revealing of bacteriophage infection strategies. Sci Rep 9:2958","journal-title":"Sci Rep"},{"key":"6_CR31","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1016\/0003-2697(82)90673-X","volume":"119","author":"CM Tsai","year":"1982","unstructured":"Tsai CM, Frasch CE (1982) A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem 119:115\u2013119","journal-title":"Anal Biochem"},{"key":"6_CR32","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1111\/j.1432-1033.1969.tb00601.x","volume":"9","author":"C Galanos","year":"1969","unstructured":"Galanos C, Luderitz O, Westphal O (1969) A new method for the extraction of R lipopolysaccharides. Eur J Biochem 9:245\u2013249","journal-title":"Eur J Biochem"},{"key":"6_CR33","doi-asserted-by":"publisher","first-page":"831","DOI":"10.1128\/jb.155.2.831-838.1983","volume":"155","author":"RP Darveau","year":"1983","unstructured":"Darveau RP, Hancock RE (1983) Procedure for isolation of bacterial lipopolysaccharides from both smooth and rough Pseudomonas aeruginosa and Salmonella typhimurium strains. J\u00a0Bacteriol 155:831\u2013838","journal-title":"J Bacteriol"},{"key":"6_CR34","doi-asserted-by":"publisher","first-page":"5144","DOI":"10.1021\/bi0020426","volume":"40","author":"U Baxa","year":"2001","unstructured":"Baxa U, Cooper A, Weintraub A et al (2001) Enthalpic barriers to the hydrophobic binding of oligosaccharides to phage P22 tailspike protein. Biochemistry 40:5144\u20135150","journal-title":"Biochemistry"},{"key":"6_CR35","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/S1016-8478(23)10763-1","volume":"24","author":"HJ Shin","year":"2007","unstructured":"Shin HJ, Lee H, Park JD et al (2007) Kinetics\u00a0of\u00a0binding of LPS to recombinant CD14, TLR4, and MD-2 proteins. Mol Cells 24:119\u2013124","journal-title":"Mol Cells"},{"key":"6_CR36","doi-asserted-by":"publisher","first-page":"3144","DOI":"10.1074\/jbc.275.5.3144","volume":"275","author":"S Viriyakosol","year":"2000","unstructured":"Viriyakosol S, Mathison JC, Tobias PS, Kirkland TN (2000) Structure-function analysis of CD14 as a soluble receptor for lipopolysaccharide. J Biol Chem 275:3144\u20133149","journal-title":"J Biol Chem"},{"key":"6_CR37","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1080\/07388551.2019.1709797","volume":"40","author":"A Basauri","year":"2020","unstructured":"Basauri A, Gonzalez-Fernandez C, Fallanza M et al (2020) Biochemical interactions between LPS and LPS-binding molecules. Crit Rev Biotechnol 40:292\u2013305","journal-title":"Crit Rev Biotechnol"},{"key":"6_CR38","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1016\/S0014-5793(99)00150-7","volume":"445","author":"CJ Thomas","year":"1999","unstructured":"Thomas CJ, Surolia A (1999) Kinetics of the interaction of endotoxin with polymyxin B and its analogs: a surface plasmon resonance analysis. FEBS Lett 445:420\u2013424","journal-title":"FEBS Lett"},{"key":"6_CR39","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1016\/j.mimet.2010.04.002","volume":"82","author":"M Matsumoto","year":"2010","unstructured":"Matsumoto M, Horiuchi Y, Yamamoto A et al (2010) Lipopolysaccaride-binding peptides obtained by phage display method. J Microbiol Methods 82:54\u201358","journal-title":"J Microbiol Methods"}],"container-title":["Methods in Molecular Biology","Lipid-Protein Interactions"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5054-7_6","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T16:55:12Z","timestamp":1779209712000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5054-7_6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071650530","9781071650547"],"references-count":39,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5054-7_6","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"20 May 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}