{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T03:00:28Z","timestamp":1767322828787,"version":"3.48.0"},"publisher-location":"New York, NY","reference-count":25,"publisher":"Springer US","isbn-type":[{"value":"9781071650592","type":"print"},{"value":"9781071650608","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5060-8_16","type":"book-chapter","created":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T02:57:48Z","timestamp":1767322668000},"page":"215-224","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single Oocyst Selection and Whole-Genome Amplification of Cryptosporidium parvum"],"prefix":"10.1007","author":[{"given":"Yuancai","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaihui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianying","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huikai","family":"Qin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zi","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junqiang","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rongjun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Longxian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,1,2]]},"reference":[{"issue":"9888","key":"16_CR1","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1016\/S0140-6736(13)60844-2","volume":"382","author":"KL Kotloff","year":"2013","unstructured":"Kotloff KL, Nataro JP, Blackwelder WC, Nasrin D, Farag TH, Panchalingam S, Wu Y, Sow SO, Sur D, Breiman RF, Faruque AS, Zaidi AK, Saha D, Alonso PL, Tamboura B, Sanogo D, Onwuchekwa U, Manna B, Ramamurthy T, Kanungo S, Ochieng JB, Omore R, Oundo JO, Hossain A, Das SK, Ahmed S, Qureshi S, Quadri F, Adegbola RA, Antonio M, Hossain MJ, Akinsola A, Mandomando I, Nhampossa T, Ac\u00e1cio S, Biswas K, O\u2019Reilly CE, Mintz ED, Berkeley LY, Muhsen K, Sommerfelt H, Robins-Browne RM, Levine MM (2013) Burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the Global Enteric Multicenter Study, GEMS): a prospective, case-control study. Lancet 382(9888):209\u2013222. https:\/\/doi.org\/10.1016\/S0140-6736(13)60844-2","journal-title":"Lancet"},{"key":"16_CR2","doi-asserted-by":"publisher","unstructured":"Ryan UM, Feng Y, Fayer R, Xiao L (2021) Taxonomy and molecular epidemiology of Cryptosporidium and Giardia \u2013 a 50 year perspective (1971\u20132021). Int J Parasitol 51(13\u201314):1099\u20131119. https:\/\/doi.org\/10.1016\/j.ijpara.2021.08.007","DOI":"10.1016\/j.ijpara.2021.08.007"},{"issue":"9","key":"16_CR3","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1111\/pim.12350","volume":"38","author":"U Ryan","year":"2016","unstructured":"Ryan U, Zahedi A, Paparini A (2016) Cryptosporidium in humans and animals-a one health approach to prophylaxis. Parasite Immunol 38(9):535\u2013547. https:\/\/doi.org\/10.1111\/pim.12350","journal-title":"Parasite Immunol"},{"key":"16_CR4","doi-asserted-by":"publisher","first-page":"F1000 Faculty R","DOI":"10.12688\/f1000research.15333.1","volume":"7","author":"S Bhalchandra","year":"2018","unstructured":"Bhalchandra S, Cardenas D, Ward HD (2018) Recent breakthroughs and ongoing limitations in Cryptosporidium research. F1000Res 7:F1000 Faculty Rev-1380. https:\/\/doi.org\/10.12688\/f1000research.15333.1","journal-title":"F1000Res"},{"issue":"8","key":"16_CR5","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1080\/14787210.2018.1500457","volume":"16","author":"MA Chavez","year":"2018","unstructured":"Chavez MA, White AC Jr (2018) Novel treatment strategies and drugs in development for cryptosporidiosis. Expert Rev Anti-Infect Ther 16(8):655\u2013661. https:\/\/doi.org\/10.1080\/14787210.2018.1500457","journal-title":"Expert Rev Anti-Infect Ther"},{"issue":"12","key":"16_CR6","doi-asserted-by":"publisher","first-page":"3195","DOI":"10.1007\/s00436-019-06537-x","volume":"118","author":"Y Fan","year":"2019","unstructured":"Fan Y, Feng Y, Xiao L (2019) Comparative genomics: how has it advanced our knowledge of cryptosporidiosis epidemiology? Parasitol Res 118(12):3195\u20133204. https:\/\/doi.org\/10.1007\/s00436-019-06537-x","journal-title":"Parasitol Res"},{"key":"16_CR7","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.actatropica.2017.10.023","volume":"184","author":"A Khan","year":"2018","unstructured":"Khan A, Shaik JS, Grigg ME (2018) Genomics and molecular epidemiology of Cryptosporidium species. Acta Trop 184:1\u201314. https:\/\/doi.org\/10.1016\/j.actatropica.2017.10.023","journal-title":"Acta Trop"},{"issue":"5","key":"16_CR8","doi-asserted-by":"publisher","first-page":"525","DOI":"10.3347\/kjp.2013.51.5.525","volume":"51","author":"KU Kim","year":"2013","unstructured":"Kim KU, Park SK, Kang SA, Park MK, Cho MK, Jung HJ, Kim KY, Yu HS (2013) Comparison of functional gene annotation of Toxascaris leonina and Toxocara canis using CLC genomics workbench. Korean J Parasitol 51(5):525\u2013530. https:\/\/doi.org\/10.3347\/kjp.2013.51.5.525","journal-title":"Korean J Parasitol"},{"issue":"5669","key":"16_CR9","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1126\/science.1094786","volume":"304","author":"MS Abrahamsen","year":"2004","unstructured":"Abrahamsen MS, Templeton TJ, Enomoto S, Abrahante JE, Zhu G, Lancto CA, Deng M, Liu C, Widmer G, Tzipori S, Buck GA, Xu P, Bankier AT, Dear PH, Konfortov BA, Spriggs HF, Iyer L, Anantharaman V, Aravind L, Kapur V (2004) Complete genome sequence of the apicomplexan, Cryptosporidium parvum. Science 304(5669):441\u2013445. https:\/\/doi.org\/10.1126\/science.1094786","journal-title":"Science"},{"issue":"6","key":"16_CR10","doi-asserted-by":"publisher","first-page":"1213","DOI":"10.1016\/j.meegid.2012.03.027","volume":"12","author":"G Widmer","year":"2012","unstructured":"Widmer G, Lee Y, Hunt P, Martinelli A, Tolkoff M, Bodi K (2012) Comparative genome analysis of two Cryptosporidium parvum isolates with different host range. Infect Genet Evol 12(6):1213\u20131221. https:\/\/doi.org\/10.1016\/j.meegid.2012.03.027","journal-title":"Infect Genet Evol"},{"issue":"7012","key":"16_CR11","doi-asserted-by":"publisher","first-page":"1107","DOI":"10.1038\/nature02977","volume":"431","author":"P Xu","year":"2004","unstructured":"Xu P, Widmer G, Wang Y, Ozaki LS, Alves JM, Serrano MG, Puiu D, Manque P, Akiyoshi D, Mackey AJ, Pearson WR, Dear PH, Bankier AT, Peterson DL, Abrahamsen MS, Kapur V, Tzipori S, Buck GA (2004) The genome of Cryptosporidium hominis. Nature 431(7012):1107\u20131112. https:\/\/doi.org\/10.1038\/nature02977","journal-title":"Nature"},{"issue":"3","key":"16_CR12","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1016\/s0304-4017(00)00315-0","volume":"92","author":"E Entrala","year":"2000","unstructured":"Entrala E, Molina-Molina J, Rosales-Lombardo M, S\u00e1nchez-Moreno M, Mascar\u00f3-Lazcano C (2000) Cryptosporidium parvum: oocysts purification using potassium bromide discontinuous gradient. Vet Parasitol 92(3):223\u2013226. https:\/\/doi.org\/10.1016\/s0304-4017(00)00315-0","journal-title":"Vet Parasitol"},{"issue":"1","key":"16_CR13","doi-asserted-by":"publisher","first-page":"650","DOI":"10.1186\/s12864-015-1805-9","volume":"16","author":"SJ Hadfield","year":"2015","unstructured":"Hadfield SJ, Pachebat JA, Swain MT, Robinson G, Cameron SJ, Alexander J, Hegarty MJ, Elwin K, Chalmers RM (2015) Generation of whole genome sequences of new Cryptosporidium hominis and Cryptosporidium parvum isolates directly from stool samples. BMC Genomics 16(1):650. https:\/\/doi.org\/10.1186\/s12864-015-1805-9","journal-title":"BMC Genomics"},{"key":"16_CR14","doi-asserted-by":"publisher","unstructured":"Power ML, Shanker SR, Sangster NC, Veal DA (2003) Evaluation of a combined immunomagnetic separation\/flow cytometry technique for epidemiological investigations of Cryptosporidium in domestic and Australian native animals. Vet Parasitol 112(1\u20132):21\u201331. https:\/\/doi.org\/10.1016\/s0304-4017(02)00414-4","DOI":"10.1016\/s0304-4017(02)00414-4"},{"issue":"8","key":"16_CR15","doi-asserted-by":"publisher","first-page":"16897","DOI":"10.3390\/ijms160816897","volume":"16","author":"A Gross","year":"2015","unstructured":"Gross A, Schoendube J, Zimmermann S, Steeb M, Zengerle R, Koltay P (2015) Technologies for Single-Cell Isolation. Int J Mol Sci 16(8):16897\u201316919. https:\/\/doi.org\/10.3390\/ijms160816897","journal-title":"Int J Mol Sci"},{"key":"16_CR16","first-page":"V","volume":"1123","author":"A Birbrair","year":"2019","unstructured":"Birbrair A (2019) Stem cells heterogeneity \u2013 novel concepts preface. Adv Exp Med Biol 1123:V\u2013Vi","journal-title":"Adv Exp Med Biol"},{"issue":"Suppl","key":"16_CR17","doi-asserted-by":"publisher","first-page":"S7","DOI":"10.1016\/j.molmet.2019.06.015","volume":"27S","author":"G Dominguez-Gutierrez","year":"2019","unstructured":"Dominguez-Gutierrez G, Xin Y, Gromada J (2019) Heterogeneity of human pancreatic \u03b2-cells. Mol Metab 27S(Suppl):S7\u2013S14. https:\/\/doi.org\/10.1016\/j.molmet.2019.06.015","journal-title":"Mol Metab"},{"issue":"4","key":"16_CR18","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1038\/s41577-019-0238-0","volume":"20","author":"N Kadouri","year":"2020","unstructured":"Kadouri N, Nevo S, Goldfarb Y, Abramson J (2020) Thymic epithelial cell heterogeneity: TEC by TEC. Nat Rev Immunol 20(4):239\u2013253. https:\/\/doi.org\/10.1038\/s41577-019-0238-0","journal-title":"Nat Rev Immunol"},{"issue":"4","key":"16_CR19","doi-asserted-by":"publisher","first-page":"395","DOI":"10.1038\/nmeth.4179","volume":"14","author":"TM Gierahn","year":"2017","unstructured":"Gierahn TM, Wadsworth MH, Hughes TK, Bryson BD, Butler A, Satija R, Fortune S, Love JC, Shalek AK (2017) Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput. Nat Methods 14(4):395\u2013398. https:\/\/doi.org\/10.1038\/nmeth.4179","journal-title":"Nat Methods"},{"key":"16_CR20","doi-asserted-by":"publisher","unstructured":"Troell K, Hallstr\u00f6m B, Divne AM, Alsmark C, Arrighi R, Huss M, Beser J, Bertilsson S (2016) Cryptosporidium as a testbed for single cell genome characterization of unicellular eukaryotes. BMC Genomics. https:\/\/doi.org\/10.1186\/s12864-016-2815-y","DOI":"10.1186\/s12864-016-2815-y"},{"issue":"1","key":"16_CR21","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2313210120","volume":"121","author":"S Shaw","year":"2024","unstructured":"Shaw S, Cohn IS, Baptista RP, Xia G, Melillo B, Agyabeng-Dadzie F, Kissinger JC, Striepen B (2024) Genetic crosses within and between species of cryptosporidium. Proc Natl Acad Sci USA 121(1):e2313210120. https:\/\/doi.org\/10.1073\/pnas.2313210120","journal-title":"Proc Natl Acad Sci USA"},{"key":"16_CR22","doi-asserted-by":"publisher","unstructured":"Heyman MB, Shigekuni LK, Ammann AJ (1986) Separation of Cryptosporidium oocysts from fecal debris by density gradient centrifugation and glass bead columns. J Clin Microbiol 23(4):789\u2013791. https:\/\/doi.org\/10.1128\/jcm.23.4.789-791.1986","DOI":"10.1128\/jcm.23.4.789-791.1986"},{"issue":"3","key":"16_CR23","doi-asserted-by":"publisher","first-page":"505","DOI":"10.4269\/ajtmh.1987.36.505","volume":"36","author":"RT Kilani","year":"1987","unstructured":"Kilani RT, Sekla L (1987) Purification of Cryptosporidium oocysts and sporozoites by cesium chloride and Percoll gradients. Am J Trop Med Hyg 36(3):505\u2013508. https:\/\/doi.org\/10.4269\/ajtmh.1987.36.505","journal-title":"Am J Trop Med Hyg"},{"issue":"1","key":"16_CR24","doi-asserted-by":"publisher","first-page":"1287","DOI":"10.1038\/s41597-024-04150-x","volume":"11","author":"Y Chen","year":"2024","unstructured":"Chen Y, Huang J, Qin H, Zhang K, Fu Y, Li J, Wang R, Chen K, Xiong J, Miao W, Wang G, Zhang L (2024) Chromosome-level genome assembly of Cryptosporidium parvum by long-read sequencing of ten oocysts. Sci Data 11(1):1287. https:\/\/doi.org\/10.1038\/s41597-024-04150-x","journal-title":"Sci Data"},{"issue":"11","key":"16_CR25","doi-asserted-by":"publisher","first-page":"4437","DOI":"10.1128\/AAC.49.11.4437-4442.2005","volume":"49","author":"X Cai","year":"2005","unstructured":"Cai X, Woods KM, Upton SJ, Zhu G (2005) Application of quantitative real-time reverse transcription-PCR in assessing drug efficacy against the intracellular pathogen Cryptosporidium parvum in vitro. Antimicrob Agents Chemother 49(11):4437\u20134442. https:\/\/doi.org\/10.1128\/AAC.49.11.4437-4442.2005","journal-title":"Antimicrob Agents Chemother"}],"container-title":["Methods in Molecular Biology","Whole Genome Amplification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5060-8_16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T02:57:49Z","timestamp":1767322669000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5060-8_16"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071650592","9781071650608"],"references-count":25,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5060-8_16","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"2 January 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}