{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T22:22:59Z","timestamp":1787696579551,"version":"build-2784847793"},"publisher-location":"New York, NY","reference-count":16,"publisher":"Springer US","isbn-type":[{"value":"9781071650714","type":"print"},{"value":"9781071650721","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5072-1_1","type":"book-chapter","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T22:03:06Z","timestamp":1787695386000},"page":"3-21","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enzymatic, Nondestructive Sequencing of 5-Methylcytosine with Direct Methylation Sequencing (DM-Seq)"],"prefix":"10.1007","author":[{"given":"Ruiyao","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian E.","family":"Loo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahul M.","family":"Kohli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,2]]},"reference":[{"key":"1_CR1","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1038\/nature14192","volume":"517","author":"D Schubeler","year":"2015","unstructured":"Schubeler D (2015) Function and information content of DNA methylation. Nature 517:321\u2013326","journal-title":"Nature"},{"key":"1_CR2","doi-asserted-by":"publisher","first-page":"1336","DOI":"10.1126\/science.aat6806","volume":"361","author":"C Luo","year":"2018","unstructured":"Luo C, Hajkova P, Ecker JR (2018) Dynamic DNA methylation: in the right place at the right time. Science 361:1336\u20131340","journal-title":"Science"},{"key":"1_CR3","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1007\/978-3-031-11454-0_4","volume":"1389","author":"RZ Jurkowska","year":"2022","unstructured":"Jurkowska RZ, Jeltsch A (2022) Enzymology of mammalian DNA methyltransferases. Adv Exp Med Biol 1389:69\u2013110","journal-title":"Adv Exp Med Biol"},{"key":"1_CR4","doi-asserted-by":"publisher","first-page":"472","DOI":"10.1038\/nature12750","volume":"502","author":"RM Kohli","year":"2013","unstructured":"Kohli RM, Zhang Y (2013) TET enzymes, TDG and the dynamics of DNA demethylation. Nature 502:472\u2013479","journal-title":"Nature"},{"key":"1_CR5","doi-asserted-by":"publisher","first-page":"2240","DOI":"10.1021\/cr5002904","volume":"115","author":"MJ Booth","year":"2015","unstructured":"Booth MJ, Raiber EA, Balasubramanian S (2015) Chemical methods for decoding cytosine modifications in DNA. Chem Rev 115:2240\u20132254","journal-title":"Chem Rev"},{"key":"1_CR6","doi-asserted-by":"crossref","unstructured":"Wang T, Loo CE, Kohli RM (2021) Enzymatic approaches for profiling cytosine methylation and hydroxymethylation. Mol Metab 57:101314","DOI":"10.1016\/j.molmet.2021.101314"},{"key":"1_CR7","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1016\/j.cell.2013.03.008","volume":"153","author":"H Shen","year":"2013","unstructured":"Shen H, Laird PW (2013) Interplay between the cancer genome and epigenome. Cell 153:38\u201355","journal-title":"Cell"},{"key":"1_CR8","doi-asserted-by":"publisher","first-page":"e8888","DOI":"10.1371\/journal.pone.0008888","volume":"5","author":"Y Huang","year":"2010","unstructured":"Huang Y, Pastor WA, Shen Y et al (2010) The behaviour of 5-hydroxymethylcytosine in bisulfite sequencing. PLoS One 5:e8888","journal-title":"PLoS One"},{"key":"1_CR9","doi-asserted-by":"publisher","first-page":"1912","DOI":"10.1016\/j.bmcl.2007.01.040","volume":"17","author":"K Tanaka","year":"2007","unstructured":"Tanaka K, Okamoto A (2007) Degradation of DNA by bisulfite treatment. Bioorg Med Chem Lett 17:1912\u20131915","journal-title":"Bioorg Med Chem Lett"},{"key":"1_CR10","doi-asserted-by":"publisher","first-page":"1083","DOI":"10.1038\/nbt.4204","volume":"36","author":"EK Schutsky","year":"2018","unstructured":"Schutsky EK, DeNizio JE, Hu P et al (2018) Nondestructive, base-resolution sequencing of 5-hydroxymethylcytosine using a DNA deaminase. Nat Biotech 36:1083\u20131090","journal-title":"Nat Biotech"},{"key":"1_CR11","doi-asserted-by":"publisher","first-page":"7655","DOI":"10.1093\/nar\/gkx345","volume":"45","author":"EK Schutsky","year":"2017","unstructured":"Schutsky EK, Nabel CS, Davis AKF et al (2017) APOBEC3A efficiently deaminates methylated, but not TET-oxidized, cytosine bases in DNA. Nucleic Acids Res 45:7655\u20137665","journal-title":"Nucleic Acids Res"},{"key":"1_CR12","doi-asserted-by":"publisher","first-page":"1280","DOI":"10.1101\/gr.266551.120","volume":"31","author":"R Vaisvila","year":"2021","unstructured":"Vaisvila R, Ponnaluri VKC, Sun Z et al (2021) Enzymatic methyl sequencing detects DNA methylation at single-base resolution from picograms of DNA. Genome Res 31:1280\u20131289","journal-title":"Genome Res"},{"key":"1_CR13","doi-asserted-by":"publisher","first-page":"424","DOI":"10.1038\/s41587-019-0041-2","volume":"37","author":"Y Liu","year":"2019","unstructured":"Liu Y, Siejka-Zielinska P, Velikova G et al (2019) Bisulfite-free direct detection of 5-methylcytosine and 5-hydroxymethylcytosine at base resolution. Nat Biotechnol 37:424\u2013429","journal-title":"Nat Biotechnol"},{"key":"1_CR14","doi-asserted-by":"publisher","first-page":"1004","DOI":"10.1038\/s41589-023-01318-1","volume":"19","author":"T Wang","year":"2023","unstructured":"Wang T, Fowler JM, Liu L et al (2023) Direct enzymatic sequencing of 5-methylcytosine at single-base resolution. Nat Chem Bio 19:1004\u20131012","journal-title":"Nat Chem Bio"},{"key":"1_CR15","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/j.chembiol.2020.09.006","volume":"28","author":"T Wang","year":"2021","unstructured":"Wang T, Kohli RM (2021) Discovery of an unnatural DNA modification derived from a natural secondary metabolite. Cell Chem Biol 28:97\u2013104","journal-title":"Cell Chem Biol"},{"key":"1_CR16","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1007\/978-1-0716-0876-0_27","volume":"2198","author":"T Wang","year":"2021","unstructured":"Wang T, Luo M, Berrios KN et al (2021) Bisulfite-free sequencing of 5-hydroxymethylcytosine with APOBEC-coupled epigenetic sequencing (ACE-Seq). Methods Mol Biol 2198:349\u2013367","journal-title":"Methods Mol Biol"}],"container-title":["Methods in Molecular Biology","Cancer Epigenetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5072-1_1","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T22:03:07Z","timestamp":1787695387000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5072-1_1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071650714","9781071650721"],"references-count":16,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5072-1_1","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"2 July 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}