{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T04:59:58Z","timestamp":1770094798221,"version":"3.49.0"},"publisher-location":"New York, NY","reference-count":11,"publisher":"Springer US","isbn-type":[{"value":"9781071651452","type":"print"},{"value":"9781071651469","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5146-9_11","type":"book-chapter","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T15:36:29Z","timestamp":1770046589000},"page":"167-177","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protein Localization with Streptavidin-Imaging: Boost Your Signal, Reduce Noise, Overcome Antibody Accessibility Problems, and Time-Travel to the Past"],"prefix":"10.1007","author":[{"given":"Johanna","family":"Krenzer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernardo Papini","family":"Gabiatti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silke","family":"Braune","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siqi","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Zoltner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne","family":"Kramer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,2,3]]},"reference":[{"key":"11_CR1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.95028.3","volume":"13","author":"J Odenwald","year":"2024","unstructured":"Odenwald J, Gabiatti B, Braune S et al (2024) Detection of TurboID fusion proteins by fluorescent streptavidin outcompetes antibody signals and visualises targets not accessible to antibodies. elife 13:RP95028","journal-title":"elife"},{"key":"11_CR2","doi-asserted-by":"publisher","first-page":"880","DOI":"10.1038\/nbt.4201","volume":"36","author":"TC Branon","year":"2018","unstructured":"Branon TC, Bosch JA, Sanchez AD et al (2018) Efficient proximity labeling in living cells and organisms with TurboID. Nat Biotechnol 36:880\u2013887","journal-title":"Nat Biotechnol"},{"key":"11_CR3","doi-asserted-by":"publisher","DOI":"10.3390\/cells9051070","volume":"9","author":"DG May","year":"2020","unstructured":"May DG, Scott KL, Campos AR et al (2020) Comparative application of BioID and TurboID for protein-proximity Biotinylation. Cells 9:1070","journal-title":"Cells"},{"key":"11_CR4","doi-asserted-by":"publisher","first-page":"7520","DOI":"10.1093\/nar\/gkad497","volume":"51","author":"S Kramer","year":"2023","unstructured":"Kramer S, Karolak NK, Odenwald J et al (2023) A unique mRNA decapping complex in trypanosomes. Nucleic Acids Res 51:7520\u20137540","journal-title":"Nucleic Acids Res"},{"key":"11_CR5","first-page":"289","volume":"36","author":"R Brun","year":"1979","unstructured":"Brun R, Sch\u00f6nenberger (1979) Cultivation and in vitro cloning or procyclic culture forms of Trypanosoma brucei in a semi-defined medium. Short communication. Acta Trop 36:289\u2013292","journal-title":"Acta Trop"},{"key":"11_CR6","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1038\/s41564-022-01295-6","volume":"8","author":"K Billington","year":"2023","unstructured":"Billington K, Halliday C, Madden R et al (2023) Genome-wide subcellular protein map for the flagellate parasite Trypanosoma brucei. Nat Microbiol 8:533\u2013547","journal-title":"Nat Microbiol"},{"key":"11_CR7","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.140197","volume":"5","author":"S Dean","year":"2015","unstructured":"Dean S, Sunter J, Wheeler RJ et al (2015) A toolkit enabling efficient, scalable and reproducible gene tagging in trypanosomatids. Open Biol 5:140197","journal-title":"Open Biol"},{"key":"11_CR8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035167","volume":"7","author":"J Sunter","year":"2012","unstructured":"Sunter J, Wickstead B, Gull K et al (2012) A new generation of T7 RNA polymerase-independent inducible expression plasmids for Trypanosoma brucei. PLoS One 7:e35167","journal-title":"PLoS One"},{"key":"11_CR9","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.32467","volume":"7","author":"J d F Nascimento","year":"2018","unstructured":"Nascimento J d F, Kelly S, Sunter J et al (2018) Codon choice directs constitutive mRNA levels in trypanosomes. elife 7:e32467","journal-title":"elife"},{"key":"11_CR10","doi-asserted-by":"publisher","first-page":"987","DOI":"10.1038\/nbt.3625","volume":"34","author":"PW Tillberg","year":"2016","unstructured":"Tillberg PW, Chen F, Piatkevich KD et al (2016) Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies. Nat Biotechnol 34:987\u2013992","journal-title":"Nat Biotechnol"},{"key":"11_CR11","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1038\/s41592-018-0238-1","volume":"16","author":"D Gambarotto","year":"2019","unstructured":"Gambarotto D, Zwettler FU, Guennec ML et al (2019) Imaging cellular ultrastructures using expansion microscopy (U-ExM). Nat Methods 16:71\u201374","journal-title":"Nat Methods"}],"container-title":["Methods in Molecular Biology","Euglenozoa"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5146-9_11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T15:36:30Z","timestamp":1770046590000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5146-9_11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071651452","9781071651469"],"references-count":11,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5146-9_11","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"3 February 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}