{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T08:51:43Z","timestamp":1791535903192,"version":"4.3.4"},"publisher-location":"New York, NY","reference-count":36,"publisher":"Springer US","isbn-type":[{"value":"9781071651612","type":"print"},{"value":"9781071651629","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5162-9_13","type":"book-chapter","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T22:03:14Z","timestamp":1787608994000},"page":"155-167","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Analysis of Secreted Proteins from Macrophomina phaseolina Isolated from Infected Glycine max Tissues"],"prefix":"10.1007","author":[{"given":"B.","family":"Ortiz-Acu\u00f1a","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Lafuente-Gamarra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. E.","family":"Bar\u00faa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. E.","family":"Flores-Giubi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. C. M.","family":"Iehisa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. C.","family":"Romero-Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,2]]},"reference":[{"key":"13_CR1","doi-asserted-by":"publisher","first-page":"25","DOI":"10.3390\/jof11010025","volume":"11","author":"C Madhushan","year":"2025","unstructured":"Madhushan C, Weerasingha A, Ilyukhin DB et al (2025) From natural hosts to agricultural threats: the Evolutionary Journey of Phytopathogenic Fungi. J Fungi 11:25","journal-title":"J Fungi"},{"key":"13_CR2","doi-asserted-by":"publisher","first-page":"414","DOI":"10.1111\/j.1364-3703.2011.00783.x","volume":"13","author":"R Dean","year":"2012","unstructured":"Dean R, Van Kan JAL, Pretorius ZA et al (2012) The top 10 fungal pathogens in molecular plant pathology. Mol Plant Pathol 13:414\u2013430.  https:\/\/doi.org\/10.1111\/j.1364-3703.2011.00783.x","journal-title":"Mol Plant Pathol"},{"key":"13_CR3","doi-asserted-by":"publisher","first-page":"430","DOI":"10.1038\/s41559-018-0793-y","volume":"3","author":"S Savary","year":"2019","unstructured":"Savary S, Willocquet L, Pethybridge SJ et al (2019) The global burden of pathogens and pests on major food crops. Nat Ecol Evol 3:430\u2013439. https:\/\/doi.org\/10.1038\/s41559-018-0793-y","journal-title":"Nat Ecol Evol"},{"key":"13_CR4","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1111\/J.1365-3059.2010.02407.X","volume":"60","author":"MW Shaw","year":"2011","unstructured":"Shaw MW, Osborne TM (2011) Geographic distribution of plant pathogens in response to climate change. Plant Pathol 60:31\u201343. https:\/\/doi.org\/10.1111\/J.1365-3059.2010.02407.X","journal-title":"Plant Pathol"},{"key":"13_CR5","doi-asserted-by":"publisher","first-page":"1264569","DOI":"10.3389\/FPLS.2023.1264569","volume":"14","author":"M Shirai","year":"2023","unstructured":"Shirai M, Eulgem T (2023) Molecular interactions between the soilborne pathogenic fungus Macrophomina phaseolina and its host plants. Front Plant Sci 14:1264569. https:\/\/doi.org\/10.3389\/FPLS.2023.1264569","journal-title":"Front Plant Sci"},{"key":"13_CR6","doi-asserted-by":"publisher","unstructured":"Dell\u2019Olmo E, Tripodi P, Zaccardelli M, Sigillo L (2022) Occurrence of Macrophomina phaseolina on chickpea in Italy: pathogen identification and characterization. Pathogens 11(8):842. https:\/\/doi.org\/10.3390\/pathogens11080842","DOI":"10.3390\/pathogens11080842"},{"key":"13_CR7","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1007\/s41348-020-00374-2","volume":"128","author":"AK Pandey","year":"2021","unstructured":"Pandey AK, Basandrai AK (2021) Will Macrophomina phaseolina spread in legumes due to climate change? A critical review of current knowledge. J Plant Dis Prot 128:9\u201318","journal-title":"J Plant Dis Prot"},{"key":"13_CR8","doi-asserted-by":"publisher","first-page":"1512294","DOI":"10.3389\/FPLS.2025.1512294","volume":"16","author":"PF Farag","year":"2025","unstructured":"Farag PF, Hussien D, Alkhalifah M et al (2025) Impact of climate change on the potential global prevalence of Macrophomina phaseolina (Tassi) Goid. under several climatological scenarios. Front Plant Sci 16:1512294. https:\/\/doi.org\/10.3389\/FPLS.2025.1512294","journal-title":"Front Plant Sci"},{"key":"13_CR9","doi-asserted-by":"publisher","first-page":"666","DOI":"10.3389\/FPLS.2021.634397\/BIBTEX","volume":"12","author":"N Marquez","year":"2021","unstructured":"Marquez N, Giachero ML, Declerck S, Ducasse DA (2021) Macrophomina phaseolina: general characteristics of pathogenicity and methods of control. Front Plant Sci 12:666. https:\/\/doi.org\/10.3389\/FPLS.2021.634397\/BIBTEX","journal-title":"Front Plant Sci"},{"key":"13_CR10","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/JPH.12854","volume":"168","author":"S Lodha","year":"2020","unstructured":"Lodha S, Mawar R (2020) Population dynamics of Macrophomina phaseolina in relation to disease management: a review. J Phytopath 168:1\u201317. https:\/\/doi.org\/10.1111\/JPH.12854","journal-title":"J Phytopath"},{"key":"13_CR11","doi-asserted-by":"publisher","first-page":"332","DOI":"10.3390\/JOF6040332","volume":"6","author":"VH Khambhati","year":"2020","unstructured":"Khambhati VH, Abbas HK, Sulyok M et al (2020) First report of the production of mycotoxins and other secondary metabolites by Macrophomina phaseolina (Tassi) Goid. isolates from soybeans (Glycine max L.) symptomatic with charcoal rot disease. J Fungi 6:332. https:\/\/doi.org\/10.3390\/JOF6040332","journal-title":"J Fungi"},{"key":"13_CR12","doi-asserted-by":"publisher","first-page":"645","DOI":"10.3390\/TOXINS11110645","volume":"11","author":"HK Abbas","year":"2019","unstructured":"Abbas HK, Bellaloui N, Accinelli C et al (2019) Toxin production in soybean (Glycine max L.) plants with charcoal rot disease and by Macrophomina phaseolina, the fungus that causes the disease. Toxins (Basel) 11:645. https:\/\/doi.org\/10.3390\/TOXINS11110645","journal-title":"Toxins (Basel)"},{"key":"13_CR13","doi-asserted-by":"publisher","first-page":"623","DOI":"10.3389\/FMICB.2022.847832\/BIBTEX","volume":"13","author":"N Sinha","year":"2022","unstructured":"Sinha N, Patra SK, Ghosh S (2022) Secretome analysis of Macrophomina phaseolina identifies an array of putative virulence factors responsible for charcoal rot disease in plants. Front Microbiol 13:623. https:\/\/doi.org\/10.3389\/FMICB.2022.847832\/BIBTEX","journal-title":"Front Microbiol"},{"key":"13_CR14","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/S00203-021-02631-W","volume":"204","author":"N Sinha","year":"2021","unstructured":"Sinha N, Patra SK, Sarkar TS, Ghosh S (2021) Secretome analysis identified extracellular superoxide dismutase and catalase of Macrophomina phaseolina. Arch Microbiol 204:1\u201311. https:\/\/doi.org\/10.1007\/S00203-021-02631-W","journal-title":"Arch Microbiol"},{"key":"13_CR15","doi-asserted-by":"publisher","first-page":"1043","DOI":"10.1016\/J.FUNBIO.2023.04.001","volume":"127","author":"A Pineda-Fretez","year":"2023","unstructured":"Pineda-Fretez A, Orrego A, Iehisa JCM et al (2023) Secretome analysis of the phytopathogen Macrophomina phaseolina cultivated in liquid medium supplemented with and without soybean leaf infusion. Fungal Biol 127:1043\u20131052. https:\/\/doi.org\/10.1016\/J.FUNBIO.2023.04.001","journal-title":"Fungal Biol"},{"key":"13_CR16","doi-asserted-by":"publisher","first-page":"1373","DOI":"10.1016\/J.TIBTECH.2020.04.013","volume":"38","author":"L Daneshmandi","year":"2020","unstructured":"Daneshmandi L, Shah S, Jafari T et al (2020) Emergence of the stem cell secretome in regenerative Engineering. Trends Biotechnol 38:1373\u20131384. https:\/\/doi.org\/10.1016\/J.TIBTECH.2020.04.013","journal-title":"Trends Biotechnol"},{"key":"13_CR17","doi-asserted-by":"publisher","unstructured":"Jia M, Gong X, Fan M, Liu H, Zhou H, Gu S, Liu Y, Dong J (2023) Identification and analysis of the secretome of plant pathogenic fungi reveals lifestyle adaptation. Front Microbiol 14:1171618. https:\/\/doi.org\/10.3389\/fmicb.2023.1171618","DOI":"10.3389\/fmicb.2023.1171618"},{"key":"13_CR18","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1002\/pmic.201200282","volume":"13","author":"V Girard","year":"2013","unstructured":"Girard V, Dieryckx C, Job C, Job D (2013) Secretomes: the fungal strike force. Proteomics 13:597\u2013608.\u2002https:\/\/doi.org\/10.1002\/pmic.201200282","journal-title":"Proteomics"},{"key":"13_CR19","doi-asserted-by":"publisher","first-page":"995","DOI":"10.3389\/FPLS.2021.687713\/BIBTEX","volume":"12","author":"D Shao","year":"2021","unstructured":"Shao D, Smith DL, Kabbage M, Roth MG (2021) Effectors of plant necrotrophic fungi. Front Plant Sci 12:995. https:\/\/doi.org\/10.3389\/FPLS.2021.687713\/BIBTEX","journal-title":"Front Plant Sci"},{"key":"13_CR20","doi-asserted-by":"publisher","first-page":"397","DOI":"10.3390\/JOF11050397","volume":"11","author":"Z Hao","year":"2025","unstructured":"Hao Z, Pan L, Xu J et al (2025) FGSE02, a novel secreted protein in Fusarium graminearum FG-12, leads to cell death in plant tissues and modulates fungal virulence. J Fungi 11:397. https:\/\/doi.org\/10.3390\/JOF11050397","journal-title":"J Fungi"},{"key":"13_CR21","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1111\/PPA.13477","volume":"71","author":"N Kondratev","year":"2022","unstructured":"Kondratev N, Middleditch MJ, Denton-Giles M et al (2022) The secreted proteome of necrotrophic Ciborinia camelliae causes nonhost-specific virulence. Plant Pathol 71:437\u2013445. https:\/\/doi.org\/10.1111\/PPA.13477","journal-title":"Plant Pathol"},{"key":"13_CR22","doi-asserted-by":"publisher","first-page":"924","DOI":"10.1016\/j.fgb.2005.08.002","volume":"42","author":"MB Su\u00e1rez","year":"2005","unstructured":"Su\u00e1rez MB, Sanz L, Chamorro MI et al (2005) Proteomic analysis of secreted proteins from Trichoderma harzianum: identification of a fungal cell wall-induced aspartic protease. Fungal Genet Biol 42:924\u2013934. https:\/\/doi.org\/10.1016\/j.fgb.2005.08.002","journal-title":"Fungal Genet Biol"},{"key":"13_CR23","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1104\/PP.17.00375","volume":"175","author":"W Zhu","year":"2017","unstructured":"Zhu W, Ronen M, Gur Y et al (2017) BcXYG1, a secreted xyloglucanase from Botrytis cinerea, triggers both cell death and plant immune responses. Plant Physiol 175:438\u2013456. https:\/\/doi.org\/10.1104\/PP.17.00375","journal-title":"Plant Physiol"},{"key":"13_CR24","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1111\/J.1364-3703.2011.00759.X","volume":"13","author":"F Yang","year":"2012","unstructured":"Yang F, Jensen JD, Svensson B et al (2012) Secretomics identifies Fusarium graminearum proteins involved in the interaction with barley and wheat. Mol Plant Pathol 13:445. https:\/\/doi.org\/10.1111\/J.1364-3703.2011.00759.X","journal-title":"Mol Plant Pathol"},{"key":"13_CR25","doi-asserted-by":"publisher","first-page":"246","DOI":"10.3390\/BIOM9060246","volume":"9","author":"T Li","year":"2019","unstructured":"Li T, Wu Y, Wang Y et al (2019) Secretome profiling reveals virulence-associated proteins of Fusarium proliferatum during Interaction with banana fruit. Biomolecules 9:246. https:\/\/doi.org\/10.3390\/BIOM9060246","journal-title":"Biomolecules"},{"key":"13_CR26","doi-asserted-by":"publisher","first-page":"807","DOI":"10.1111\/jph.12138","volume":"161","author":"ME Flores-Giubi","year":"2013","unstructured":"Flores-Giubi ME, Brito-Arg\u00e1ez L, Garc\u00eda-Sosa K et al (2013) Optimization of culturing conditions of a strain of Phytophthora capsici pathogenic to habanero pepper (Capsicum chinense). J Phytopath 161:807\u2013813. https:\/\/doi.org\/10.1111\/jph.12138","journal-title":"J Phytopath"},{"key":"13_CR27","doi-asserted-by":"publisher","first-page":"2782","DOI":"10.1002\/ELPS.200500722","volume":"27","author":"W Wang","year":"2006","unstructured":"Wang W, Vignani R, Scali M, Cresti M (2006) A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis. Electrophoresis 27:2782\u20132786. https:\/\/doi.org\/10.1002\/ELPS.200500722","journal-title":"Electrophoresis"},{"key":"13_CR28","doi-asserted-by":"publisher","first-page":"839","DOI":"10.3389\/fpls.2015.00839","volume":"6","author":"R Gonz\u00e1lez-Fern\u00e1ndez","year":"2015","unstructured":"Gonz\u00e1lez-Fern\u00e1ndez R, Valero-Galv\u00e1n J, G\u00f3mez-G\u00e1lvez FJ, Jorr\u00edn-Novo JV (2015) Unraveling the in vitro secretome of the phytopathogen Botrytis cinerea to understand the interaction with its hosts. Front Plant Sci 6:839. https:\/\/doi.org\/10.3389\/fpls.2015.00839","journal-title":"Front Plant Sci"},{"key":"13_CR29","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1016\/0003-2697(84)90782-6","volume":"138","author":"D Wessel","year":"1984","unstructured":"Wessel D, Fl\u00fcgge UI (1984) A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. Anal Biochem 138:141\u2013143. https:\/\/doi.org\/10.1016\/0003-2697(84)90782-6","journal-title":"Anal Biochem"},{"key":"13_CR30","doi-asserted-by":"publisher","first-page":"680","DOI":"10.1038\/227680a0","volume":"227","author":"UK Laemmli","year":"1970","unstructured":"Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680\u2013685","journal-title":"Nature"},{"key":"13_CR31","doi-asserted-by":"publisher","first-page":"4007","DOI":"10.1016\/S0021-9258(19)41496-8","volume":"250","author":"P O\u2019Farrell","year":"1975","unstructured":"O\u2019Farrell P (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007\u20134021","journal-title":"J Biol Chem"},{"key":"13_CR32","doi-asserted-by":"publisher","first-page":"1170","DOI":"10.1038\/S41374-022-00830-7","volume":"102","author":"M Cui","year":"2022","unstructured":"Cui M, Cheng C, Zhang L (2022) High-throughput proteomics: a methodological mini-review. Lab Invest 102:1170\u20131181. https:\/\/doi.org\/10.1038\/S41374-022-00830-7","journal-title":"Lab Invest"},{"key":"13_CR33","doi-asserted-by":"publisher","first-page":"2369","DOI":"10.1002\/ELPS.200305500","volume":"24","author":"W Wang","year":"2003","unstructured":"Wang W, Scali M, Vignani R et al (2003) Protein extraction for two-dimensional electrophoresis from olive leaf, a plant tissue containing high levels of interfering compounds. Electrophoresis 24:2369\u20132375. https:\/\/doi.org\/10.1002\/ELPS.200305500","journal-title":"Electrophoresis"},{"key":"13_CR34","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","volume":"72","author":"MM Bradford","year":"1976","unstructured":"Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248\u2013254","journal-title":"Anal Biochem"},{"key":"13_CR35","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1002\/ELPS.1150090603","volume":"9","author":"V Neuhoff","year":"1988","unstructured":"Neuhoff V, Arold N, Taube D, Ehrhardt W (1988) Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 9:255\u2013262. https:\/\/doi.org\/10.1002\/ELPS.1150090603","journal-title":"Electrophoresis"},{"key":"13_CR36","doi-asserted-by":"publisher","first-page":"979","DOI":"10.1007\/S11119-019-09704-3\/METRICS","volume":"21","author":"F Morlin Carneiro","year":"2020","unstructured":"Morlin Carneiro F, Angeli Furlani CE, Zerbato C et al (2020) Comparison between vegetation indices for detecting spatial and temporal variabilities in soybean crop using canopy sensors. Precis Agric 21:979\u20131007. https:\/\/doi.org\/10.1007\/S11119-019-09704-3\/METRICS","journal-title":"Precis Agric"}],"container-title":["Methods in Molecular Biology","Plant Biotic Stress"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5162-9_13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T08:24:52Z","timestamp":1791534292000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5162-9_13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071651612","9781071651629"],"references-count":36,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5162-9_13","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"2 July 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}},{"value":"The authors have no conflicts of interest to declare that are relevant to the content of this chapter.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}}]}}