{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T23:50:17Z","timestamp":1785628217773,"version":"3.56.0"},"publisher-location":"New York, NY","reference-count":15,"publisher":"Springer US","isbn-type":[{"value":"9781071651650","type":"print"},{"value":"9781071651667","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5166-7_15","type":"book-chapter","created":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T23:18:26Z","timestamp":1785626306000},"page":"227-238","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Purification of Protein-Free and Protein-Linked Poly(ADP-Ribose) from Biochemical Reactions and from Human Cells"],"prefix":"10.1007","author":[{"given":"Marie-France","family":"Langelier","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manija","family":"Mirhasan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M.","family":"Pascal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,2]]},"reference":[{"key":"15_CR1","doi-asserted-by":"publisher","unstructured":"L\u00fcscher B, Ahel I, Altmeyer M et al (2021) ADP-ribosyltransferases, an update on function and nomenclature. FEBS J. https:\/\/doi.org\/10.1111\/febs.16142","DOI":"10.1111\/febs.16142"},{"key":"15_CR2","doi-asserted-by":"publisher","first-page":"105397","DOI":"10.1016\/J.JBC.2023.105397","volume":"299","author":"\u00c9 Rouleau-Turcotte","year":"2023","unstructured":"Rouleau-Turcotte \u00c9, Pascal JM (2023) ADP-ribose contributions to genome stability and PARP enzyme trapping on sites of DNA damage; paradigm shifts for a coming-of-age modification. J Biol Chem 299:105397. https:\/\/doi.org\/10.1016\/J.JBC.2023.105397","journal-title":"J Biol Chem"},{"key":"15_CR3","doi-asserted-by":"publisher","first-page":"1097","DOI":"10.1042\/BCJ20230230","volume":"481","author":"M Jessop","year":"2024","unstructured":"Jessop M, Broadway BJ, Miller K, Guettler S (2024) Regulation of PARP1\/2 and the tankyrases: emerging parallels. Biochem J 481:1097\u20131123. https:\/\/doi.org\/10.1042\/BCJ20230230","journal-title":"Biochem J"},{"key":"15_CR4","doi-asserted-by":"publisher","first-page":"1092","DOI":"10.1021\/acs.chemrev.7b00122","volume":"118","author":"B L\u00fcscher","year":"2018","unstructured":"L\u00fcscher B, B\u00fctepage M, Eckei L et al (2018) ADP-ribosylation, a multifaceted posttranslational modification involved in the control of cell physiology in health and disease. Chem Rev 118:1092\u20131136. https:\/\/doi.org\/10.1021\/acs.chemrev.7b00122","journal-title":"Chem Rev"},{"key":"15_CR5","doi-asserted-by":"publisher","first-page":"4475","DOI":"10.1016\/J.CELL.2023.08.030","volume":"186","author":"MJ Suskiewicz","year":"2023","unstructured":"Suskiewicz MJ, Prokhorova E, Rack JGM, Ahel I (2023) ADP-ribosylation from molecular mechanisms to therapeutic implications. Cell 186:4475\u20134495. https:\/\/doi.org\/10.1016\/J.CELL.2023.08.030","journal-title":"Cell"},{"key":"15_CR6","doi-asserted-by":"publisher","unstructured":"Palazzo L, Leidecker O, Prokhorova E et al (2018) Serine is the major residue for ADP-ribosylation upon DNA damage. Elife 7. https:\/\/doi.org\/10.7554\/eLife.34334","DOI":"10.7554\/eLife.34334"},{"key":"15_CR7","doi-asserted-by":"publisher","first-page":"8432","DOI":"10.1093\/NAR\/GKAB618","volume":"49","author":"JM Reber","year":"2021","unstructured":"Reber JM, Mangerich A (2021) Why structure and chain length matter: on the biological significance underlying the structural heterogeneity of poly(ADP-ribose). Nucleic Acids Res 49:8432\u20138448. https:\/\/doi.org\/10.1093\/NAR\/GKAB618","journal-title":"Nucleic Acids Res"},{"key":"15_CR8","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/S0022-2836(02)00946-4","volume":"323","author":"JF Rippmann","year":"2002","unstructured":"Rippmann JF, Damm K, Schnapp A (2002) Functional characterization of the poly(ADP-ribose) polymerase activity of tankyrase 1, a potential regulator of telomere length. J Mol Biol 323:217\u2013224","journal-title":"J Mol Biol"},{"key":"15_CR9","doi-asserted-by":"publisher","first-page":"801200.","DOI":"10.3389\/fcell.2021.801200","volume":"9","author":"IA Richard","year":"2022","unstructured":"Richard IA, Burgess JT, O\u2019Byrne KJ, Bolderson E (2022) Beyond PARP1: the potential of other members of the poly (ADP-ribose) polymerase family in DNA repair and cancer therapeutics. Front Cell Dev Biol 9:801200","journal-title":"Front Cell Dev Biol"},{"key":"15_CR10","doi-asserted-by":"publisher","unstructured":"Langelier MF, Mirhasan M, Gilbert K et al (2024) PARP enzyme de novo synthesis of protein-free poly(ADP-ribose). Mol Cell 84. https:\/\/doi.org\/10.1016\/J.MOLCEL.2024.10.024","DOI":"10.1016\/J.MOLCEL.2024.10.024"},{"key":"15_CR11","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1016\/j.ab.2012.06.015","volume":"428","author":"ES Tan","year":"2012","unstructured":"Tan ES, Krukenberg KA, Mitchison TJ (2012) Large-scale preparation and characterization of poly(ADP-ribose) and defined length polymers. Anal Biochem 428:126\u2013136. https:\/\/doi.org\/10.1016\/j.ab.2012.06.015","journal-title":"Anal Biochem"},{"key":"15_CR12","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1111\/J.1432-1033.1981.TB06303.X","volume":"117","author":"K Wielckens","year":"1981","unstructured":"Wielckens K, Bredehorst R, Adamietz P, Hilz H (1981) Protein-bound polymeric and monomeric ADP-ribose residues in hepatic tissues. Comparative analyses using a new procedure for the quantification of poly(ADP-ribose). Eur J Biochem 117:69\u201374. https:\/\/doi.org\/10.1111\/J.1432-1033.1981.TB06303.X","journal-title":"Eur J Biochem"},{"key":"15_CR13","doi-asserted-by":"publisher","unstructured":"Juarez-Salinas H, Sims JL, Jacobson MK (1979) Poly(ADP-ribose) levels in carcinogen-treated cells. Nature 282(5740):740\u2013741. https:\/\/doi.org\/10.1038\/282740a0","DOI":"10.1038\/282740a0"},{"key":"15_CR14","doi-asserted-by":"publisher","unstructured":"Longarini EJ, Mati\u0107 I (2024) Preserving ester-linked modifications reveals glutamate and aspartate mono-ADP-ribosylation by PARP1 and its reversal by PARG. Nat Commun 15. https:\/\/doi.org\/10.1038\/S41467-024-48314-0","DOI":"10.1038\/S41467-024-48314-0"},{"key":"15_CR15","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1007\/978-1-61779-270-0_13","volume":"780","author":"M-F Langelier","year":"2011","unstructured":"Langelier M-F, Planck JL, Servent KM, Pascal JM (2011) Purification of human PARP-1 and PARP-1 domains from Escherichia coli for structural and biochemical analysis. Methods Mol Biol 780:209\u2013226. https:\/\/doi.org\/10.1007\/978-1-61779-270-0_13","journal-title":"Methods Mol Biol"}],"container-title":["Methods in Molecular Biology","Protein Post-Translational Modifications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5166-7_15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T23:18:26Z","timestamp":1785626306000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5166-7_15"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071651650","9781071651667"],"references-count":15,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5166-7_15","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"2 July 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}