{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T16:33:51Z","timestamp":1786120431253,"version":"build-2736575974"},"publisher-location":"New York, NY","reference-count":50,"publisher":"Springer US","isbn-type":[{"value":"9781071651896","type":"print"},{"value":"9781071651902","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5190-2_7","type":"book-chapter","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T22:07:25Z","timestamp":1786054045000},"page":"101-126","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Droplet-Based Microfluidics Methods for Detecting Enzyme Inhibitors"],"prefix":"10.1007","author":[{"given":"Abraham","family":"Ochoa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frida","family":"Trejo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis F.","family":"Olgu\u00edn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,2]]},"reference":[{"key":"7_CR1","doi-asserted-by":"publisher","first-page":"7964","DOI":"10.1021\/acs.chemrev.6b00848","volume":"117","author":"L Shang","year":"2017","unstructured":"Shang L, Cheng Y, Zhao Y (2017) Emerging droplet microfluidics. Chem Rev 117:7964\u20138040. https:\/\/doi.org\/10.1021\/acs.chemrev.6b00848","journal-title":"Chem Rev"},{"key":"7_CR2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s43586-023-00212-3","volume":"3","author":"T Moragues","year":"2023","unstructured":"Moragues T, Arguijo D, Beneyton T et al (2023) Droplet-based microfluidics. Nat Rev Methods Prim 3:1\u201322. https:\/\/doi.org\/10.1038\/s43586-023-00212-3","journal-title":"Nat Rev Methods Prim"},{"key":"7_CR3","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1007\/s10404-008-0271-y","volume":"5","author":"Z Nie","year":"2008","unstructured":"Nie Z, Seo MS, Xu S et al (2008) Emulsification in a microfluidic flow-focusing device: effect of the viscosities of the liquids. Microfluid Nanofluid 5:585\u2013594. https:\/\/doi.org\/10.1007\/s10404-008-0271-y","journal-title":"Microfluid Nanofluid"},{"key":"7_CR4","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1021\/la990101e","volume":"16","author":"PB Umbanhowar","year":"2000","unstructured":"Umbanhowar PB, Prasad V, Weitz DA (2000) Monodisperse emulsion generation via drop\u00a0break off in a coflowing stream. Langmuir\u00a016:347\u2013351. https:\/\/doi.org\/10.1021\/la990101e","journal-title":"Langmuir"},{"key":"7_CR5","doi-asserted-by":"publisher","first-page":"5955","DOI":"10.1021\/nn401661d","volume":"7","author":"JU Shim","year":"2013","unstructured":"Shim JU, Ranasinghe RT, Smith CA et al (2013) Ultrarapid generation of femtoliter microfluidic droplets for single-molecule-counting immunoassays. ACS Nano 7:5955\u20135964. https:\/\/doi.org\/10.1021\/nn401661d","journal-title":"ACS Nano"},{"key":"7_CR6","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1021\/acs.analchem.5b04139","volume":"88","author":"AK Price","year":"2016","unstructured":"Price AK, Paegel BM (2016) Discovery in droplets. Anal Chem 88:339\u2013353. https:\/\/doi.org\/10.1021\/acs.analchem.5b04139","journal-title":"Anal Chem"},{"key":"7_CR7","doi-asserted-by":"publisher","first-page":"5571","DOI":"10.1021\/acs.chemrev.2c00910","volume":"123","author":"M Gantz","year":"2023","unstructured":"Gantz M, Neun S, Medcalf EJ et al (2023) Ultrahigh-throughput enzyme engineering and discovery in in vitro compartments. Chem Rev 123:5571\u20135611. https:\/\/doi.org\/10.1021\/acs.chemrev.2c00910","journal-title":"Chem Rev"},{"key":"7_CR8","doi-asserted-by":"publisher","first-page":"1707","DOI":"10.1039\/b821021g","volume":"9","author":"N Damean","year":"2009","unstructured":"Damean N, Olguin LF, Hollfelder F et al (2009) Simultaneous measurement of reactions in microdroplets filled by concentration gradients. Lab Chip 9:1707\u20131713. https:\/\/doi.org\/10.1039\/b821021g","journal-title":"Lab Chip"},{"key":"7_CR9","doi-asserted-by":"publisher","unstructured":"Gielen F, van Vliet L, Koprowski BT et al (2013) A fully unsupervised compartment-on-demand platform for precise nanoliter assays oftime-dependent steady-state enzyme kinetics and inhibition. Anal Chem 85:4761\u20134769. https:\/\/doi.org\/10.1021\/ac400480z","DOI":"10.1021\/ac400480z"},{"key":"7_CR10","doi-asserted-by":"publisher","first-page":"1754","DOI":"10.1039\/c3lc41398e","volume":"13","author":"SL Sjostrom","year":"2013","unstructured":"Sjostrom SL, Joensson HN, Svahn HA (2013) Multiplex analysis of enzyme kinetics and inhibition by droplet microfluidics using picoinjectors. Lab Chip 13:1754\u20131761. https:\/\/doi.org\/10.1039\/c3lc41398e","journal-title":"Lab Chip"},{"key":"7_CR11","doi-asserted-by":"publisher","first-page":"4965","DOI":"10.1021\/acs.analchem.5b00766","volume":"87","author":"D Hess","year":"2015","unstructured":"Hess D, Rane A, Demello AJ, Stavrakis S (2015) High-throughput, quantitative enzyme kinetic analysis in microdroplets using stroboscopic epifluorescence imaging. Anal Chem 87:4965\u20134972. https:\/\/doi.org\/10.1021\/acs.analchem.5b00766","journal-title":"Anal Chem"},{"key":"7_CR12","doi-asserted-by":"publisher","first-page":"1066","DOI":"10.1016\/j.chempr.2021.02.011","volume":"7","author":"D Hess","year":"2021","unstructured":"Hess D, Dockalova V, Kokkonen P et al (2021) Exploring mechanism of enzyme catalysis by on-chip transient kinetics coupled with global data analysis and molecular modeling. Chem 7:1066\u20131079. https:\/\/doi.org\/10.1016\/j.chempr.2021.02.011","journal-title":"Chem"},{"key":"7_CR13","doi-asserted-by":"publisher","first-page":"2854","DOI":"10.1039\/d2lc01143c","volume":"23","author":"N Lobato-Dauzier","year":"2023","unstructured":"Lobato-Dauzier N, Deteix R, Gines G et al (2023) Silicon chambers for enhanced incubation and imaging of microfluidic droplets. Lab Chip 23:2854\u20132865. https:\/\/doi.org\/10.1039\/d2lc01143c","journal-title":"Lab Chip"},{"key":"7_CR14","doi-asserted-by":"publisher","unstructured":"Vazquez B, Qureshi N, Oropeza-Ramos L, Olguin LF (2014) Effect of velocity on microdroplet fluorescence quantified by laser-induced fluorescence. Lab Chip 14:3550\u20133555. https:\/\/doi.org\/10.1039\/c4lc00654b","DOI":"10.1039\/c4lc00654b"},{"key":"7_CR15","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1016\/j.aca.2013.04.064","volume":"787","author":"Y Zhu","year":"2013","unstructured":"Zhu Y, Fang Q (2013) Analytical detection techniques for droplet microfluidics \u2013 a review. Anal Chim Acta 787:24\u201335. https:\/\/doi.org\/10.1016\/j.aca.2013.04.064","journal-title":"Anal Chim Acta"},{"key":"7_CR16","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1146\/annurev-anchem-061516-045219","volume":"10","author":"OJ Dressler","year":"2017","unstructured":"Dressler OJ, Casadevall i Solvas X, deMello AJ (2017) Chemical and biological dynamics using droplet-based microfluidics. Annu Rev Anal Chem 10:1\u201324. https:\/\/doi.org\/10.1146\/annurev-anchem-061516-045219","journal-title":"Annu Rev Anal Chem"},{"key":"7_CR17","doi-asserted-by":"publisher","first-page":"4597","DOI":"10.1021\/acs.analchem.2c04144","volume":"95","author":"EJ Medcalf","year":"2023","unstructured":"Medcalf EJ, Gantz M, Kaminski TS, Hollfelder F (2023) Ultra-high-throughput absorbance-activated droplet sorting for enzyme screening at kilohertz frequencies. Anal Chem 95:4597\u20134604. https:\/\/doi.org\/10.1021\/acs.analchem.2c04144","journal-title":"Anal Chem"},{"key":"7_CR18","doi-asserted-by":"publisher","unstructured":"Jain A, Teshima M, Buryska T et al (2024) High-throughput absorbance-activated droplet sorting for engineering aldehyde dehydrogenases. Angew Chemie Int Ed 63(49):e202409610. https:\/\/doi.org\/10.1002\/anie.202409610","DOI":"10.1002\/anie.202409610"},{"key":"7_CR19","doi-asserted-by":"publisher","first-page":"9309","DOI":"10.1021\/ac502542z","volume":"86","author":"S Sun","year":"2014","unstructured":"Sun S, Kennedy RRT (2014) Droplet electrospray ionization mass spectrometry for high throughput screening for enzyme inhibitors. Anal Chem 86:9309\u20139314. https:\/\/doi.org\/10.1021\/ac502542z","journal-title":"Anal Chem"},{"key":"7_CR20","doi-asserted-by":"publisher","first-page":"102962","DOI":"10.1016\/j.copbio.2023.102962","volume":"82","author":"BE Murray","year":"2023","unstructured":"Murray BE, Penabad LI, Kennedy RT (2023) Advances in coupling droplet microfluidics to mass spectrometry. Curr Opin Biotechnol 82:102962. https:\/\/doi.org\/10.1016\/j.copbio.2023.102962","journal-title":"Curr Opin Biotechnol"},{"key":"7_CR21","doi-asserted-by":"publisher","first-page":"5840","DOI":"10.1021\/ac900811y","volume":"81","author":"Z Han","year":"2009","unstructured":"Han Z, Li W, Huang Y, Zheng B (2009) Measuring rapid enzymatic kinetics by electrochemical method in droplet-based microfluidic devices with pneumatic valves. Anal Chem 81:5840\u20135845. https:\/\/doi.org\/10.1021\/ac900811y","journal-title":"Anal Chem"},{"key":"7_CR22","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1177\/2211068214549625","volume":"20","author":"Z Mao","year":"2015","unstructured":"Mao Z, Guo F, Xie Y et al (2015) Label-free measurements of reaction kinetics using a droplet-based optofluidic device. J Lab Autom 20:17\u201324. https:\/\/doi.org\/10.1177\/2211068214549625","journal-title":"J Lab Autom"},{"key":"7_CR23","doi-asserted-by":"publisher","first-page":"1912","DOI":"10.1038\/nprot.2017.092","volume":"12","author":"A Baccouche","year":"2017","unstructured":"Baccouche A, Okumura S, Sieskind R et al (2017) Massively parallel and multiparameter titration of biochemical assays with droplet microfluidics. Nat Protoc 12:1912\u20131932. https:\/\/doi.org\/10.1038\/nprot.2017.092","journal-title":"Nat Protoc"},{"key":"7_CR24","doi-asserted-by":"publisher","first-page":"130227","DOI":"10.1016\/j.colsurfa.2022.130227","volume":"655","author":"X Chen","year":"2022","unstructured":"Chen X, Hou L, Zhang Z et al (2022) Microfluidic encapsulation of soluble reagents with large-scale concentration gradients in a sequence of droplets for comparative analysis. Colloids Surf A Physicochem Eng Asp 655:130227. https:\/\/doi.org\/10.1016\/j.colsurfa.2022.130227","journal-title":"Colloids Surf A Physicochem Eng Asp"},{"key":"7_CR25","doi-asserted-by":"publisher","first-page":"340587","DOI":"10.1016\/j.aca.2022.340587","volume":"1236","author":"MA Ahamed","year":"2022","unstructured":"Ahamed MA, Kim G, Li Z, Kim SJ (2022) Pre-programmed microdroplet generator to control wide-ranging chemical concentrations. Anal Chim Acta 1236:340587. https:\/\/doi.org\/10.1016\/j.aca.2022.340587","journal-title":"Anal Chim Acta"},{"key":"7_CR26","doi-asserted-by":"publisher","unstructured":"Cai L-F, Zhu Y, Du G-S, Fang Q (2012) Droplet-based microfluidic flow injection system with large-scale concentration gradient by a single nanoliter-scale injection for enzyme inhibition assay. Anal Chem 84:446\u2013452. https:\/\/doi.org\/10.1021\/ac2029198","DOI":"10.1021\/ac2029198"},{"key":"7_CR27","doi-asserted-by":"publisher","first-page":"378","DOI":"10.1073\/pnas.1113324109\/-\/DCSupplemental.www.pnas.org\/cgi\/doi\/10.1073\/pnas.1113324109","volume":"109","author":"OJ Miller","year":"2012","unstructured":"Miller OJ, El A, Mangeat T et al (2012) High-resolution dose\u2014response screening using droplet-based microfluidics. Proc Natl Acad Sci 109:378\u2013383. https:\/\/doi.org\/10.1073\/pnas.1113324109\/-\/DCSupplemental.www.pnas.org\/cgi\/doi\/10.1073\/pnas.1113324109","journal-title":"Proc Natl Acad Sci"},{"key":"7_CR28","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1016\/j.aca.2013.08.016","volume":"796","author":"S Gu","year":"2013","unstructured":"Gu S, Lu Y, Ding Y et al (2013) Droplet-based microfluidics for dose-response assay of enzyme inhibitors by electrochemical method. Anal Chim Acta 796:68\u201374. https:\/\/doi.org\/10.1016\/j.aca.2013.08.016","journal-title":"Anal Chim Acta"},{"key":"7_CR29","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1039\/c3an01917a","volume":"139","author":"X-L Wang","year":"2014","unstructured":"Wang X-L, Zhu Y, Fang Q (2014) Coupling liquid chromatography\/mass spectrometry detection with microfluidic droplet array for label-free enzyme inhibition assay. Analyst 139:191\u2013197. https:\/\/doi.org\/10.1039\/c3an01917a","journal-title":"Analyst"},{"key":"7_CR30","doi-asserted-by":"publisher","first-page":"1950","DOI":"10.1021\/ac504330x","volume":"87","author":"TD Rane","year":"2015","unstructured":"Rane TD, Zec HC, Wang TH (2015) A barcode-free combinatorial screening platform for matrix metalloproteinase screening. Anal Chem 87:1950\u20131956. https:\/\/doi.org\/10.1021\/ac504330x","journal-title":"Anal Chem"},{"key":"7_CR31","doi-asserted-by":"publisher","first-page":"136162","DOI":"10.1016\/j.snb.2024.136162","volume":"417","author":"A Samimi","year":"2024","unstructured":"Samimi A, Hengoju S, Rosenbaum MA (2024) Combinatorial sample preparation platform for droplet-based applications in microbiology. Sens Actuators B Chem 417:136162. https:\/\/doi.org\/10.1016\/j.snb.2024.136162","journal-title":"Sens Actuators B Chem"},{"key":"7_CR32","doi-asserted-by":"publisher","first-page":"4735","DOI":"10.1039\/d2lc00909a","volume":"22","author":"GK Zath","year":"2022","unstructured":"Zath GK, Sperling RA, Hoffman CW et al (2022) Rapid parallel generation of a fluorescently barcoded drop library from a microtiter plate using the plate-interfacing parallel encapsulation (PIPE) chip. Lab Chip 22:4735\u20134745. https:\/\/doi.org\/10.1039\/d2lc00909a","journal-title":"Lab Chip"},{"key":"7_CR33","doi-asserted-by":"publisher","first-page":"518","DOI":"10.1021\/acscombsci.5b00106","volume":"17","author":"AB MacConnell","year":"2015","unstructured":"MacConnell AB, McEnaney PJ, Cavett VJ, Paegel BM (2015) DNA-encoded solid-phase synthesis: encoding language design and complex oligomer library synthesis. ACS Comb Sci 17:518\u2013534. https:\/\/doi.org\/10.1021\/acscombsci.5b00106","journal-title":"ACS Comb Sci"},{"key":"7_CR34","doi-asserted-by":"publisher","first-page":"2904","DOI":"10.1021\/acs.analchem.5b04811","volume":"88","author":"AK Price","year":"2016","unstructured":"Price AK, MacConnell AB, Paegel BM (2016) H\u03bdSABR: photochemical dose-response bead screening in droplets. Anal Chem 88:2904\u20132911. https:\/\/doi.org\/10.1021\/acs.analchem.5b04811","journal-title":"Anal Chem"},{"key":"7_CR35","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1021\/acscombsci.9b00037","volume":"21","author":"WG Cochrane","year":"2019","unstructured":"Cochrane WG, Malone ML, Dang VQ et al (2019) Activity-based DNA-encoded library screening. ACS Comb Sci 21:425\u2013435. https:\/\/doi.org\/10.1021\/acscombsci.9b00037","journal-title":"ACS Comb Sci"},{"key":"7_CR36","doi-asserted-by":"publisher","first-page":"1295","DOI":"10.1021\/acsmedchemlett.3c00159","volume":"14","author":"PR Fitzgerald","year":"2023","unstructured":"Fitzgerald PR, Cochrane WG, Paegel BM (2023) Dose-response activity-based DNA-encoded library screening. ACS Med Chem Lett 14:1295\u20131303. https:\/\/doi.org\/10.1021\/acsmedchemlett.3c00159","journal-title":"ACS Med Chem Lett"},{"key":"7_CR37","doi-asserted-by":"publisher","first-page":"3449","DOI":"10.1021\/ac1005316","volume":"82","author":"AB Theberge","year":"2010","unstructured":"Theberge AB, Whyte G, Huck WTS (2010) Generation of picoliter droplets with defined contents and concentration gradients from the separation of chemical mixtures. Anal Chem 82:3449\u20133453. https:\/\/doi.org\/10.1021\/ac1005316","journal-title":"Anal Chem"},{"key":"7_CR38","doi-asserted-by":"publisher","first-page":"11811","DOI":"10.1021\/ac5033963","volume":"86","author":"ASA Keyon","year":"2014","unstructured":"Keyon ASA, Guijt RM, Bolch CJ, Breadmore MC (2014) Droplet microfluidics for postcolumn reactions in capillary electrophoresis. Anal Chem 86:11811\u201311818. https:\/\/doi.org\/10.1021\/ac5033963","journal-title":"Anal Chem"},{"key":"7_CR39","doi-asserted-by":"publisher","unstructured":"Zohouri D, Lienard-Mayor T, Obeid S et al (2024) A review on hyphenation of droplet microfluidics to separation techniques: from instrumental conception to analytical applications for limited sample volumes. Anal Chim Acta 1291:342090. https:\/\/doi.org\/10.1016\/j.aca.2023.342090","DOI":"10.1016\/j.aca.2023.342090"},{"key":"7_CR40","doi-asserted-by":"publisher","first-page":"4889","DOI":"10.1021\/acs.analchem.6b04988","volume":"89","author":"A Ochoa","year":"2017","unstructured":"Ochoa A, \u00c1lvarez-Boh\u00f3rquez E, Castillero E, Olguin LF (2017) Detection of enzyme inhibitors in crude natural extracts using droplet-based microfluidics coupled to HPLC. Anal Chem 89:4889\u20134896. https:\/\/doi.org\/10.1021\/acs.analchem.6b04988","journal-title":"Anal Chem"},{"key":"7_CR41","doi-asserted-by":"publisher","first-page":"1632","DOI":"10.1039\/b806706f","volume":"8","author":"C Holtze","year":"2008","unstructured":"Holtze C, Rowat AC, Agresti JJ et al (2008) Biocompatible surfactants for water-in-fluorocarbon emulsions. Lab Chip 8:1632\u20131639. https:\/\/doi.org\/10.1039\/b806706f","journal-title":"Lab Chip"},{"key":"7_CR42","doi-asserted-by":"publisher","first-page":"3685","DOI":"10.1002\/polb.20621","volume":"43","author":"S Zhu","year":"2005","unstructured":"Zhu S, Edmonds WF, Hillmyer MA, Lodge TP (2005) Synthesis and self-assembly of highly incompatible polybutadiene-poly(hexafluoropropylene oxide) diblock copolymers. J Polym Sci Part B Polym Phys 43:3685\u20133694. https:\/\/doi.org\/10.1002\/polb.20621","journal-title":"J Polym Sci Part B Polym Phys"},{"key":"7_CR43","doi-asserted-by":"publisher","first-page":"791","DOI":"10.1038\/nchem.1996","volume":"6","author":"M Fischlechner","year":"2014","unstructured":"Fischlechner M, Schaerli Y, Mohamed MF et al (2014) Evolution of enzyme catalysts caged in biomimetic gel-shell beads. Nat Chem 6:791\u2013796. https:\/\/doi.org\/10.1038\/nchem.1996","journal-title":"Nat Chem"},{"key":"7_CR44","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1007\/s10404-012-0958-y","volume":"13","author":"K Hirata","year":"2012","unstructured":"Hirata K, Ichii T, Suzuki H et al (2012) Fractal-shaped microchannel design for a kinetic analysis of biochemical reaction in a delay line. Microfluid Nanofluid 13:273\u2013278. https:\/\/doi.org\/10.1007\/s10404-012-0958-y","journal-title":"Microfluid Nanofluid"},{"key":"7_CR45","doi-asserted-by":"publisher","first-page":"1548","DOI":"10.1039\/b610567j","volume":"6","author":"K Haubert","year":"2006","unstructured":"Haubert K, Drier T, Beebe D (2006) PDMS bonding by means of a portable, low-cost corona system. Lab Chip 6:1548\u20131549. https:\/\/doi.org\/10.1039\/b610567j","journal-title":"Lab Chip"},{"key":"7_CR46","doi-asserted-by":"publisher","unstructured":"Ferraro D, Serra M, Filippi D et al (2018) Controlling the distance of highly confined droplets in a capillary by interfacial tension for merging on-demand. Lab Chip 19 136\u2013146. https:\/\/doi.org\/10.1039\/C8LC01182F","DOI":"10.1039\/C8LC01182F"},{"key":"7_CR47","volume-title":"Evaluation of enzyme inhibitors in drug discovery","author":"RA Copeland","year":"2005","unstructured":"Copeland RA (2005) Evaluation of enzyme inhibitors in drug discovery. Wiley, Hoboken, New Jersey"},{"key":"7_CR48","doi-asserted-by":"publisher","first-page":"24115","DOI":"10.1039\/d4ra04298k","volume":"14","author":"J Waeterschoot","year":"2024","unstructured":"Waeterschoot J, Kayahan E, Breukers J et al (2024) The effects of droplet stabilization by surfactants and nanoparticles on leakage, cross-talk, droplet stability, and cell adhesion. RSC Adv 14:24115\u201324129. https:\/\/doi.org\/10.1039\/d4ra04298k","journal-title":"RSC Adv"},{"key":"7_CR49","doi-asserted-by":"publisher","first-page":"10618","DOI":"10.1039\/c2sm25934f","volume":"8","author":"Y Skhiri","year":"2012","unstructured":"Skhiri Y, Gruner P, Semin B et al (2012) Dynamics of molecular transport by surfactants in emulsions. Soft Matter 8:10618\u201310627. https:\/\/doi.org\/10.1039\/c2sm25934f","journal-title":"Soft Matter"},{"key":"7_CR50","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1016\/j.bbapap.2011.10.011","volume":"1824","author":"F Exnowitz","year":"2012","unstructured":"Exnowitz F, Meyer B, Hackl T (2012) NMR for direct determination of Km and Vmax of enzyme reactions based on the Lambert W function-analysis of progress curves. Biochim Biophys Acta - Proteins Proteomics 1824:443\u2013449. https:\/\/doi.org\/10.1016\/j.bbapap.2011.10.011","journal-title":"Biochim Biophys Acta - Proteins Proteomics"}],"container-title":["Methods in Molecular Biology","Targeting Enzymes for Pharmaceutical Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5190-2_7","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T22:07:26Z","timestamp":1786054046000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5190-2_7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071651896","9781071651902"],"references-count":50,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5190-2_7","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"2 July 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}},{"value":"The authors have no conflicts of interest to declare that are relevant to the content of this chapter.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}}]}}