{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:14:34Z","timestamp":1788304474697,"version":"build-2803163510"},"publisher-location":"New York, NY","reference-count":22,"publisher":"Springer US","isbn-type":[{"value":"9781071651933","type":"print"},{"value":"9781071651940","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5194-0_1","type":"book-chapter","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T22:24:25Z","timestamp":1788301465000},"page":"3-20","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Targeting, Mutagenesis, and Functional Testing Strategies for Large Collagens"],"prefix":"10.1007","author":[{"given":"Xinyi","family":"Bao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Bornert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Nystr\u00f6m","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,2]]},"reference":[{"key":"1_CR1","doi-asserted-by":"publisher","first-page":"e24176","DOI":"10.7554\/eLife.24176","volume":"6","author":"AL Fidler","year":"2017","unstructured":"Fidler AL, Darris CE, Chetyrkin SV et al (2017) Collagen IV and basement membrane at the evolutionary dawn of metazoan tissues. eLife 6:e24176. https:\/\/doi.org\/10.7554\/eLife.24176","journal-title":"eLife"},{"key":"1_CR2","doi-asserted-by":"publisher","first-page":"980","DOI":"10.1111\/1523-1747.ep12491894","volume":"97","author":"CL Phillips","year":"1991","unstructured":"Phillips CL, Lever LW, Pinnell SR et al (1991) Construction of a full-length murine pro alpha 2(I) collagen cDNA by the polymerase chain reaction. J Invest Dermatol 97:980\u2013984","journal-title":"J Invest Dermatol"},{"key":"1_CR3","doi-asserted-by":"publisher","first-page":"a004978","DOI":"10.1101\/cshperspect.a004978","volume":"3","author":"S Ricard-Blum","year":"2011","unstructured":"Ricard-Blum S (2011) The collagen family. Cold Spring Harb Perspect Biol 3:a004978. https:\/\/doi.org\/10.1101\/cshperspect.a004978","journal-title":"Cold Spring Harb Perspect Biol"},{"key":"1_CR4","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1136\/jmg.2006.045302","volume":"44","author":"R Varki","year":"2007","unstructured":"Varki R, Sadowski S, Uitto J, Pfendner E (2007) Epidermolysis bullosa. II. Type VII collagen mutations and phenotype-genotype correlations in the dystrophic subtypes. J Med Genet 44:181\u2013192. https:\/\/doi.org\/10.1136\/jmg.2006.045302","journal-title":"J Med Genet"},{"key":"1_CR5","doi-asserted-by":"publisher","first-page":"21649","DOI":"10.1074\/jbc.M100180200","volume":"276","author":"M Chen","year":"2001","unstructured":"Chen M, Keene DR, Costa FK et al (2001) The carboxyl terminus of type VII collagen mediates antiparallel dimer formation and constitutes a new antigenic epitope for epidermolysis Bullosa acquisita autoantibodies. J Biol Chem 276:21649\u201321655. https:\/\/doi.org\/10.1074\/jbc.M100180200","journal-title":"J Biol Chem"},{"key":"1_CR6","doi-asserted-by":"publisher","first-page":"26372","DOI":"10.1074\/jbc.M203247200","volume":"277","author":"A Rattenholl","year":"2002","unstructured":"Rattenholl A, Pappano WN, Koch M et al (2002) Proteinases of the bone morphogenetic protein-1 family convert procollagen VII to mature anchoring fibril collagen. J Biol Chem 277:26372\u201326378. https:\/\/doi.org\/10.1074\/jbc.M203247200","journal-title":"J Biol Chem"},{"key":"1_CR7","doi-asserted-by":"publisher","first-page":"24188","DOI":"10.1074\/jbc.M501486200","volume":"280","author":"C Moali","year":"2005","unstructured":"Moali C, Font B, Ruggiero F et al (2005) Substrate-specific modulation of a multisubstrate proteinase C-terminal processing of fibrillar procollagens is the only BMP-1-dependent activity to be enhanced by PCPE-1. J Biol Chem 280:24188\u201324194. https:\/\/doi.org\/10.1074\/jbc.M501486200","journal-title":"J Biol Chem"},{"key":"1_CR8","doi-asserted-by":"publisher","first-page":"3498","DOI":"10.1172\/JCI68127","volume":"123","author":"A Nystr\u00f6m","year":"2013","unstructured":"Nystr\u00f6m A, Velati D, Mittapalli VR et al (2013) Collagen VII plays a dual role in wound healing. J Clin Invest 123:3498\u20133509. https:\/\/doi.org\/10.1172\/JCI68127","journal-title":"J Clin Invest"},{"key":"1_CR9","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1016\/j.det.2009.10.020","volume":"28","author":"L Bruckner-Tuderman","year":"2010","unstructured":"Bruckner-Tuderman L (2010) Dystrophic epidermolysis bullosa: pathogenesis and clinical features. Dermatol Clin 28:107\u2013114. https:\/\/doi.org\/10.1016\/j.det.2009.10.020","journal-title":"Dermatol Clin"},{"key":"1_CR10","doi-asserted-by":"publisher","first-page":"1302","DOI":"10.1038\/mt.2016.92","volume":"24","author":"O Bornert","year":"2016","unstructured":"Bornert O, K\u00fchl T, Bremer J et al (2016) Analysis of the functional consequences of targeted exon deletion in COL7A1 reveals prospects for dystrophic epidermolysis bullosa therapy. Mol Ther J Am Soc Gene Ther 24:1302\u20131311. https:\/\/doi.org\/10.1038\/mt.2016.92","journal-title":"Mol Ther J Am Soc Gene Ther"},{"key":"1_CR11","doi-asserted-by":"publisher","unstructured":"Nystr\u00f6m A, Bernasconi R, Bornert O (2017) Therapies for genetic extracellular matrix diseases of the skin. Matrix Biol J Int Soc Matrix Biol 71-72:330-347. https:\/\/doi.org\/10.1016\/j.matbio.2017.12.010","DOI":"10.1016\/j.matbio.2017.12.010"},{"key":"1_CR12","doi-asserted-by":"publisher","first-page":"2614","DOI":"10.1038\/sj.jid.5700435","volume":"126","author":"M Goto","year":"2006","unstructured":"Goto M, Sawamura D, Nishie W et al (2006) Targeted skipping of a single exon harboring a premature termination codon mutation: implications and potential for gene correction therapy for selective dystrophic epidermolysis bullosa patients. J Invest Dermatol 126:2614\u20132620. https:\/\/doi.org\/10.1038\/sj.jid.5700435","journal-title":"J Invest Dermatol"},{"key":"1_CR13","doi-asserted-by":"publisher","first-page":"1660","DOI":"10.1073\/pnas.1614775114","volume":"114","author":"W Wu","year":"2017","unstructured":"Wu W, Lu Z, Li F et al (2017) Efficient in vivo gene editing using ribonucleoproteins in skin stem cells of recessive dystrophic epidermolysis bullosa mouse model. Proc Natl Acad Sci U S A 114:1660\u20131665. https:\/\/doi.org\/10.1073\/pnas.1614775114","journal-title":"Proc Natl Acad Sci U S A"},{"key":"1_CR14","doi-asserted-by":"publisher","first-page":"2387","DOI":"10.1016\/j.jid.2016.07.029","volume":"136","author":"S Turczynski","year":"2016","unstructured":"Turczynski S, Titeux M, Tonasso L et al (2016) Targeted exon skipping restores type VII collagen expression and anchoring fibril formation in an in vivo RDEB model. J Invest Dermatol 136:2387\u20132395. https:\/\/doi.org\/10.1016\/j.jid.2016.07.029","journal-title":"J Invest Dermatol"},{"key":"1_CR15","doi-asserted-by":"publisher","first-page":"e379","DOI":"10.1038\/mtna.2016.87","volume":"5","author":"J Bremer","year":"2016","unstructured":"Bremer J, Bornert O, Nystr\u00f6m A et al (2016) Antisense oligonucleotide-mediated exon skipping as a systemic therapeutic approach for recessive dystrophic epidermolysis bullosa. Mol Ther Nucleic Acids 5:e379. https:\/\/doi.org\/10.1038\/mtna.2016.87","journal-title":"Mol Ther Nucleic Acids"},{"key":"1_CR16","doi-asserted-by":"publisher","first-page":"e0145620","DOI":"10.1371\/journal.pone.0145620","volume":"11","author":"ZYC Toh","year":"2016","unstructured":"Toh ZYC, Thandar Aung-Htut M, Pinniger G et al (2016) Deletion of dystrophin in-frame exon 5 leads to a severe phenotype: guidance for exon skipping strategies. PloS One 11:e0145620. https:\/\/doi.org\/10.1371\/journal.pone.0145620","journal-title":"PloS One"},{"key":"1_CR17","doi-asserted-by":"publisher","first-page":"1609860","DOI":"10.3389\/pore.2021.1609860","volume":"27","author":"SE Oh","year":"2021","unstructured":"Oh SE, Oh MY, An JY et al (2021) Prognostic value of highly expressed type VII collagen (COL7A1) in patients with gastric cancer. Pathol Oncol Res POR 27:1609860. https:\/\/doi.org\/10.3389\/pore.2021.1609860","journal-title":"Pathol Oncol Res POR"},{"key":"1_CR18","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-021-09136-1","volume":"22","author":"AS Brodsky","year":"2022","unstructured":"Brodsky AS, Khurana J, Guo KS et al (2022) Somatic mutations in collagens are associated with a distinct tumor environment and overall survival in gastric cancer. BMC Cancer 22: 139. https:\/\/doi.org\/10.1186\/s12885-021-09136-1","journal-title":"BMC Cancer"},{"key":"1_CR19","doi-asserted-by":"publisher","first-page":"1368","DOI":"10.1038\/mt.2015.58","volume":"23","author":"T K\u00fchl","year":"2015","unstructured":"K\u00fchl T, Mezger M, Hausser I et al (2015) High local concentrations of intradermal MSCs restore skin integrity and facilitate wound healing in dystrophic epidermolysis bullosa. Mol Ther J Am Soc Gene Ther 23:1368\u20131379. https:\/\/doi.org\/10.1038\/mt.2015.58","journal-title":"Mol Ther J Am Soc Gene Ther"},{"key":"1_CR20","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1038\/nmeth.1318","volume":"6","author":"DG Gibson","year":"2009","unstructured":"Gibson DG, Young L, Chuang R-Y et al (2009) Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods 6:343\u2013345. https:\/\/doi.org\/10.1038\/nmeth.1318","journal-title":"Nat Methods"},{"key":"1_CR21","doi-asserted-by":"publisher","first-page":"985","DOI":"10.1084\/jem.20171626","volume":"215","author":"A Seki","year":"2018","unstructured":"Seki A, Rutz S (2018) Optimized RNP transfection for highly efficient CRISPR\/Cas9-mediated gene knockout in primary T cells. J Exp Med 215:985\u2013997. https:\/\/doi.org\/10.1084\/jem.20171626","journal-title":"J Exp Med"},{"key":"1_CR22","doi-asserted-by":"publisher","first-page":"1075","DOI":"10.1167\/iovs.17-23425","volume":"59","author":"B Wullink","year":"2018","unstructured":"Wullink B, Pas HH, Van der Worp RJ et al (2018) Type VII collagen in the human accommodation system: expression in ciliary body, zonules, and lens capsule. Invest Ophthalmol Vis Sci 59:1075\u20131083. https:\/\/doi.org\/10.1167\/iovs.17-23425","journal-title":"Invest Ophthalmol Vis Sci"}],"container-title":["Methods in Molecular Biology","Collagen"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5194-0_1","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T22:24:25Z","timestamp":1788301465000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5194-0_1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071651933","9781071651940"],"references-count":22,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5194-0_1","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"2 August 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}