{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T11:04:42Z","timestamp":1784372682641,"version":"3.55.0"},"publisher-location":"New York, NY","reference-count":22,"publisher":"Springer US","isbn-type":[{"value":"9781071652442","type":"print"},{"value":"9781071652459","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5245-9_10","type":"book-chapter","created":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T10:50:21Z","timestamp":1784371821000},"page":"171-183","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Durum Wheat Anther Culture: a Complete Protocol"],"prefix":"10.1007","author":[{"given":"Ioannis N.","family":"Xynias","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theano B.","family":"Lazaridou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,19]]},"reference":[{"key":"10_CR1","doi-asserted-by":"publisher","first-page":"432","DOI":"10.3390\/agronomy10030432","volume":"10","author":"\u0399\u039d Xynias","year":"2020","unstructured":"Xynias \u0399\u039d, Mylonas \u0399, Korpetis \u0395G et al (2020) Durum wheat breeding in the mediterranean region: current status and future prospects. Agronomy 10:432. https:\/\/doi.org\/10.3390\/agronomy10030432","journal-title":"Agronomy"},{"issue":"4","key":"10_CR2","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1007\/s11738-005-0068-5","volume":"27","author":"M Tersi","year":"2005","unstructured":"Tersi M, Xynias I, Gouli-Vavdinoudi E, Roupakias D (2005) Effect of genome, induction medium and temperature pretreatment on green plant production in durum (Triticum turgidum var durum) \u00d7 bread wheat (Triticum aestivum L em Thell) crosses. Acta Physiol Plant 27(4):641\u2013649","journal-title":"Acta Physiol Plant"},{"key":"10_CR3","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1007\/BF00292308","volume":"77","author":"S Agache","year":"1989","unstructured":"Agache S, \u0392acheller \u0392, De \u0392uyser J et al (1989) Genetic analysis of anther culture in wheat using aneuploid, chromosome substitution and translocation lines. Theor Appl Genet 77:7\u201311","journal-title":"Theor Appl Genet"},{"key":"10_CR4","doi-asserted-by":"crossref","unstructured":"Barnabas B, Szakacs E, Karsai I, Bedo Z (2001)  In vitro  androgenesis of wheat: from fundamentals to practical application. Euphytica 119:211\u2013216","DOI":"10.1023\/A:1017558825810"},{"key":"10_CR5","doi-asserted-by":"crossref","unstructured":"Lantos C, Weyen J, Orsini JM et al (2013) Efficient application of in vitro anther culture for different European winter wheat (Triticum aestivum  L) breeding programs. Plant Breed 132:149\u2013154","DOI":"10.1111\/pbr.12032"},{"key":"10_CR6","unstructured":"L\u00f6schenberger F, H\u00e4nseL H, Heberle-Bors E (1993) Plant formation from pollen of tetraploid wheat species and  Triticum tauschii. Cereal Res Commun 21(2\/3):133\u2013139"},{"key":"10_CR7","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1007\/BF00270121","volume":"7","author":"E Szakacs","year":"1988","unstructured":"Szakacs E, Kovacs G, Pauk J, Barnabas B (1988) Substitution analysis of callus induction and plant regeneration from anther culture in wheat (Triticum aestivum L.). Plant Cell Rep 7:127\u2013129","journal-title":"Plant Cell Rep"},{"key":"10_CR8","first-page":"95","volume":"58","author":"\u03a4\u0392 Lazaridou","year":"2016","unstructured":"Lazaridou \u03a4\u0392, Pankou CI, Xynias IN, Roupakias DG (2016) Effect of D genome on wheat anther culture response after cold and mannitol pre-treatment. Acta Biol Crack Ser Bot 58:95\u2013102","journal-title":"Acta Biol Crack Ser Bot"},{"key":"10_CR9","first-page":"262","volume":"5","author":"TB Lazaridou","year":"2011","unstructured":"Lazaridou TB, Sistanis I, Lithourgidis AS et al (2011) Response to in-vitro anther culture of F3 families originating from high and low yielding F2 barley (Hordeum vulgare L) plants. Aust J Crop Sci 5:262\u2013267","journal-title":"Aust J Crop Sci"},{"key":"10_CR10","first-page":"233","volume":"2","author":"J Kaushal","year":"2015","unstructured":"Kaushal J, Sharmai R, Balachandran SM et al (2015) Effect of cold pretreatment on improving anther culture response of rice (Oryza sativa L). J Exp Biol Agric Sci 2:233\u2013242","journal-title":"J Exp Biol Agric Sci"},{"key":"10_CR11","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1007\/s10681-004-3036-z","volume":"140","author":"M Patel","year":"2004","unstructured":"Patel M, Darvey NL, Marshal DR, Berry JO (2004) Optimization of culture condition for improved plant regeneration efficiency from wheat microspore culture. Euphytica 140:197\u2013204","journal-title":"Euphytica"},{"key":"10_CR12","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1007\/s00299-005-0047-8","volume":"25","author":"L Cistu\u00e9","year":"2006","unstructured":"Cistu\u00e9 L, Soriano M, Castillo AM et al (2006) Production of doubled haploids in durum wheat (Triticum turgidum var durum) through isolated microspore culture. Plant Cell Rep 25:257\u2013264. https:\/\/doi.org\/10.1007\/s00299-005-0047-8","journal-title":"Plant Cell Rep"},{"key":"10_CR13","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1111\/j.1439-0523.2007.01399.x","volume":"126","author":"Z Labbani","year":"2007","unstructured":"Labbani Z, De Buyser J, Picard E (2007) Effect of mannitol pre-treatment to improve green plant regeneration on isolated microspore culture in Triticum turgidum ssp durum cv \u2018Jennah Khetifa\u2019. Plant Breed 126:565\u2013568","journal-title":"Plant Breed"},{"issue":"6","key":"10_CR14","doi-asserted-by":"publisher","first-page":"590","DOI":"10.3103\/S0095452721060074","volume":"55","author":"TB Lazaridou","year":"2021","unstructured":"Lazaridou TB, Mavromatis AG, Xynias IN (2021) Effect of culture medium and mannitol pre-treatment on durum wheat anther culture response. Cytol Genet 55(6):590\u2013597","journal-title":"Cytol Genet"},{"key":"10_CR15","unstructured":"Hunter CP (1987) European Patent Application No 0245898 A2, Shell Int. Res: 1\u20138"},{"key":"10_CR16","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1007\/BF03543341","volume":"32","author":"JW Ouyang","year":"2004","unstructured":"Ouyang JW, Liang H, Zhanf C et al (2004) The effect of potato extract used as additive in anther culture medium on culture responses in Triticum aestivum. Cereal Res Commun 32:501\u2013508. https:\/\/doi.org\/10.1007\/BF03543341","journal-title":"Cereal Res Commun"},{"issue":"3","key":"10_CR17","doi-asserted-by":"publisher","first-page":"209","DOI":"10.5586\/asbp.2009.026","volume":"8","author":"A Ponitka","year":"2009","unstructured":"Ponitka A, Slusarkiewicz-Jarzina A (2009) Regeneration of oat androgenic plants in relation to induction media and culture conditions of embryo-like structures. Acta Soc Bot Pol 8(3):209\u2013213. https:\/\/doi.org\/10.5586\/asbp.2009.026","journal-title":"Acta Soc Bot Pol"},{"key":"10_CR18","first-page":"413","volume-title":"Cell and tissue culture techniques for cereal crop improvement","author":"J \u0396huang","year":"1983","unstructured":"\u0396huang J, Jia X (1983) Increasing differentiation frequencies in wheat pollen callus. In: Hu H, Vega MR (Eds.) Cell and tissue culture techniques for cereal crop improvement. Sinica Science Press,  Beijing, 413\u2013432"},{"key":"10_CR19","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1111\/j.1399-3054.1962.tb08052.x","volume":"15","author":"T Murashige","year":"1962","unstructured":"Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473\u2013497","journal-title":"Physiol Plant"},{"key":"10_CR20","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1111\/j.1365-3180.1974.tb01084.x","volume":"14","author":"JC Zadoks","year":"1974","unstructured":"Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Res 14:415\u2013421","journal-title":"Weed Res"},{"key":"10_CR21","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1046\/j.1439-0523.2003.00866.x","volume":"122","author":"I Zamani","year":"2003","unstructured":"Zamani I, Gouli\u2013Vavdinoudi E, Kovacs G et al (2003) Effect of parental genotypes and colchicine treatment on the androgenic response of wheat F1 hybrids. Plant Breed 122:1\u20134","journal-title":"Plant Breed"},{"key":"10_CR22","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1007\/978-94-017-1293-4_11","volume-title":"Doubled haploid production in crop plants. A manual","author":"B Barnabas","year":"2003","unstructured":"Barnabas B (2003) Protocol for producing doubled haploid plants from anther culture of wheat (Triticum aestivum L). In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (Eds.) Doubled haploid production in crop plants. A manual. Kluwer, Dordrecht, 65\u201370"}],"container-title":["Methods in Molecular Biology","Doubled Haploid Methods in Cereals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5245-9_10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T10:50:22Z","timestamp":1784371822000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5245-9_10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071652442","9781071652459"],"references-count":22,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5245-9_10","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"19 July 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}