{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T04:26:06Z","timestamp":1787113566696,"version":"build-2736575974"},"publisher-location":"New York, NY","reference-count":42,"publisher":"Springer US","isbn-type":[{"value":"9781071652879","type":"print"},{"value":"9781071652886","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5288-6_8","type":"book-chapter","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T02:51:10Z","timestamp":1787107870000},"page":"91-104","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Structure Analysis of Gangliosides: Mass Spectrometry Imaging"],"prefix":"10.1007","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7497-3540","authenticated-orcid":false,"given":"Ariful","family":"Islam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1302-6467","authenticated-orcid":false,"given":"Mitsutoshi","family":"Setou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"8_CR1","doi-asserted-by":"publisher","first-page":"124721","DOI":"10.1016\/j.talanta.2023.124721","volume":"264","author":"X Guo","year":"2023","unstructured":"Guo X, Wang X, Tian C et al (2023) Development of mass spectrometry imaging techniques and its latest applications. Talanta 264:124721","journal-title":"Talanta"},{"key":"8_CR2","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1038\/s44303-024-00025-3","volume":"2","author":"H Zhang","year":"2024","unstructured":"Zhang H, Lu KH, Ebbini M et al (2024) Mass spectrometry imaging for spatially resolved multi-omics molecular mapping. Npj Imaging 2:20","journal-title":"Npj Imaging"},{"key":"8_CR3","doi-asserted-by":"publisher","first-page":"843","DOI":"10.1080\/14789450.2020.1880897","volume":"17","author":"A Mamun","year":"2020","unstructured":"Mamun A, Islam A, Eto F et al (2020) Mass spectrometry-based phospholipid imaging: methods and findings. Expert Rev Proteomics 17:843\u2013854","journal-title":"Expert Rev Proteomics"},{"key":"8_CR4","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1007\/s12551-009-0015-6","volume":"1","author":"C Murayama","year":"2009","unstructured":"Murayama C, Kimura Y, Setou M (2009) Imaging mass spectrometry: principle and application. Biophys Rev 1:131\u2013139","journal-title":"Biophys Rev"},{"key":"8_CR5","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1038\/s41423-020-0388-9","volume":"17","author":"G Cutillo","year":"2020","unstructured":"Cutillo G, Saariaho A-H, Meri S (2020) Physiology of gangliosides and the role of antiganglioside antibodies in human diseases. Cell Mol Immunol 17:313\u2013322","journal-title":"Cell Mol Immunol"},{"key":"8_CR6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/bs.pmbts.2018.01.002","volume":"156","author":"R Sandhoff","year":"2018","unstructured":"Sandhoff R, Schulze H, Sandhoff K (2018) Ganglioside metabolism in health and disease. Prog Mol Biol Transl Sci 156:1\u201362","journal-title":"Prog Mol Biol Transl Sci"},{"key":"8_CR7","doi-asserted-by":"publisher","first-page":"572965","DOI":"10.3389\/fnins.2020.572965","volume":"14","author":"S Sipione","year":"2020","unstructured":"Sipione S, Monyror J, Galleguillos D et al (2020) Gangliosides in the brain: physiology, pathophysiology and therapeutic applications. Front Neurosci 14:572965","journal-title":"Front Neurosci"},{"key":"8_CR8","doi-asserted-by":"publisher","first-page":"1329","DOI":"10.2174\/0115665240264547231017110613","volume":"24","author":"S Kumar","year":"2024","unstructured":"Kumar S, Panda SP (2024) Targeting GM2 ganglioside accumulation in dementia: current therapeutic approaches and future directions. Curr Mol Med 24:1329\u20131345","journal-title":"Curr Mol Med"},{"key":"8_CR9","doi-asserted-by":"crossref","unstructured":"Ledeen R, Chowdhury S (2022) Gangliosides in neurodegenerative diseases. In: Glycobiology of the nervous system. Springer, pp 391\u2013418","DOI":"10.1007\/978-3-031-12390-0_13"},{"key":"8_CR10","doi-asserted-by":"publisher","first-page":"5659","DOI":"10.1007\/s00216-019-01989-7","volume":"411","author":"F Tobias","year":"2019","unstructured":"Tobias F, Pathmasiri KC, Cologna SM (2019) Mass spectrometry imaging reveals ganglioside and ceramide localization patterns during cerebellar degeneration in the Npc1\u2212\/\u2212 mouse model. Anal Bioanal Chem 411:5659\u20135668","journal-title":"Anal Bioanal Chem"},{"key":"8_CR11","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-22093-4","volume":"12","author":"KC O\u2019Neill","year":"2022","unstructured":"O\u2019Neill KC, Liapis E, Harris BT et al (2022) Mass spectrometry imaging discriminates glioblastoma tumor cell subpopulations and different microvascular formations based on their lipid profiles. Sci Rep 12: 17069","journal-title":"Sci Rep"},{"key":"8_CR12","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1021\/acs.analchem.7b04733","volume":"90","author":"AR Buchberger","year":"2018","unstructured":"Buchberger AR, DeLaney K, Johnson J et al (2018) Mass spectrometry imaging: a review of emerging advancements and future insights. Anal Chem 90:240","journal-title":"Anal Chem"},{"key":"8_CR13","doi-asserted-by":"publisher","first-page":"6869","DOI":"10.1007\/s00216-024-05584-3","volume":"416","author":"X Cui","year":"2024","unstructured":"Cui X, Geng H, Guo H et al (2024) Visualizing the transdermal delivery of berberine loaded within chitosan microneedles using mass spectrometry imaging. Anal Bioanal Chem 416:6869\u20136877","journal-title":"Anal Bioanal Chem"},{"key":"8_CR14","doi-asserted-by":"publisher","first-page":"e0283155","DOI":"10.1371\/journal.pone.0283155","volume":"18","author":"S Aramaki","year":"2023","unstructured":"Aramaki S, Tsuge S, Islam A et al (2023) Lipidomics-based tissue heterogeneity in specimens of luminal breast cancer revealed by clustering analysis of mass spectrometry imaging: a preliminary study. PLoS One 18:e0283155","journal-title":"PLoS One"},{"key":"8_CR15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41467-019-13858-z","volume":"11","author":"PD Piehowski","year":"2020","unstructured":"Piehowski PD, Zhu Y, Bramer LM et al (2020) Automated mass spectrometry imaging of over 2000 proteins from tissue sections at 100-\u03bcm spatial resolution. Nat Commun 11:1\u201312","journal-title":"Nat Commun"},{"key":"8_CR16","doi-asserted-by":"publisher","first-page":"1463","DOI":"10.1007\/s13361-018-1928-8","volume":"29","author":"SN Jackson","year":"2018","unstructured":"Jackson SN, Muller L, Roux A et al (2018) AP-MALDI mass spectrometry imaging of gangliosides using 2, 6-dihydroxyacetophenone. J Am Soc Mass Spectrom 29:1463\u20131472","journal-title":"J Am Soc Mass Spectrom"},{"key":"8_CR17","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.ymeth.2016.02.014","volume":"104","author":"K \u0160kr\u00e1\u0161kov\u00e1","year":"2016","unstructured":"\u0160kr\u00e1\u0161kov\u00e1 K, Claude E, Jones EA et al (2016) Enhanced capabilities for imaging gangliosides in murine brain with matrix-assisted laser desorption\/ionization and desorption electrospray ionization mass spectrometry coupled to ion mobility separation. Methods 104:69\u201378","journal-title":"Methods"},{"key":"8_CR18","doi-asserted-by":"publisher","first-page":"793","DOI":"10.1111\/pcmr.13182","volume":"37","author":"N Neittaanm\u00e4ki","year":"2024","unstructured":"Neittaanm\u00e4ki N, Zaar O, Cehajic KS et al (2024) ToF-SIMS imaging reveals changes in tumor cell lipids during metastatic progression of melanoma. Pigment Cell Melanoma Res 37:793\u2013800","journal-title":"Pigment Cell Melanoma Res"},{"key":"8_CR19","doi-asserted-by":"publisher","first-page":"2473","DOI":"10.1039\/C9AN02270H","volume":"145","author":"W Wang","year":"2020","unstructured":"Wang W, Whitehead S (2020) Imaging mass spectrometry allows for neuroanatomic-specific detection of gangliosides in the healthy and diseased brain. Analyst 145:2473\u20132481","journal-title":"Analyst"},{"key":"8_CR20","doi-asserted-by":"publisher","first-page":"9799","DOI":"10.1021\/acs.analchem.3c05557","volume":"96","author":"T Enzlein","year":"2024","unstructured":"Enzlein T, Lashley T, Sammour DA et al (2024) Integrative single-plaque analysis reveals signature A\u03b2 and lipid profiles in the Alzheimer\u2019s brain. Anal Chem 96:9799\u20139807","journal-title":"Anal Chem"},{"key":"8_CR21","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1007\/s00401-024-02759-1","volume":"147","author":"N Ollen-Bittle","year":"2024","unstructured":"Ollen-Bittle N, Pejhan S, Pasternak SH et al (2024) Co-registration of MALDI-MSI and histology demonstrates gangliosides co-localize with amyloid beta plaques in Alzheimer\u2019s disease. Acta Neuropathol 147:105","journal-title":"Acta Neuropathol"},{"key":"8_CR22","doi-asserted-by":"publisher","first-page":"e0176254","DOI":"10.1371\/journal.pone.0176254","volume":"12","author":"L Ermini","year":"2017","unstructured":"Ermini L, Morganti E, Post A et al (2017) Imaging mass spectrometry identifies prognostic ganglioside species in rodent intracranial transplants of glioma and medulloblastoma. PLoS One 12:e0176254","journal-title":"PLoS One"},{"key":"8_CR23","doi-asserted-by":"publisher","DOI":"10.1038\/srep25289","volume":"6","author":"Y Zhang","year":"2016","unstructured":"Zhang Y, Wang J, Liu Ja et al (2016) Combination of ESI and MALDI mass spectrometry for qualitative, semi-quantitative and in situ analysis of gangliosides in brain. Sci Rep 6: 25289","journal-title":"Sci Rep"},{"key":"8_CR24","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1002\/ansa.202000176","volume":"2","author":"BA Dilmetz","year":"2021","unstructured":"Dilmetz BA, Lee YR, Condina MR et al (2021) Novel technical developments in mass spectrometry imaging in 2020: A mini review. Anal Sci Adv 2:225\u2013237","journal-title":"Anal Sci Adv"},{"key":"8_CR25","doi-asserted-by":"publisher","first-page":"2527","DOI":"10.1021\/acs.analchem.0c04572","volume":"93","author":"ST Mezger","year":"2021","unstructured":"Mezger ST, Mingels AM, Bekers O et al (2021) Mass spectrometry spatial-omics on a single conductive slide. Anal Chem 93:2527\u20132533","journal-title":"Anal Chem"},{"key":"8_CR26","doi-asserted-by":"publisher","first-page":"546","DOI":"10.3390\/biom13030546","volume":"13","author":"J Leopold","year":"2023","unstructured":"Leopold J, Prabutzki P, Engel KM et al (2023) A five-year update on matrix compounds for MALDI-MS analysis of lipids. Biomolecules 13:546","journal-title":"Biomolecules"},{"key":"8_CR27","doi-asserted-by":"crossref","unstructured":"Sugiyama E, Setou M (2018) Visualization of brain gangliosides using MALDI imaging mass spectrometry. In: Gangliosides: methods and protocols, pp 223\u2013229","DOI":"10.1007\/978-1-4939-8552-4_10"},{"key":"8_CR28","doi-asserted-by":"crossref","unstructured":"Suzuki A, Suzuki M, Ito E et al (2018) Mass spectrometry of gangliosides. In: Gangliosides: methods and protocols, pp 207\u2013221","DOI":"10.1007\/978-1-4939-8552-4_9"},{"key":"8_CR29","doi-asserted-by":"publisher","first-page":"1692","DOI":"10.1021\/jasms.4c00089","volume":"35","author":"KV Djambazova","year":"2024","unstructured":"Djambazova KV, Gibson-Corley KN, Freiberg JA et al (2024) MALDI TIMS IMS reveals ganglioside molecular diversity within murine S. aureus kidney tissue abscesses. J Am Soc Mass Spectrom 35:1692\u20131701","journal-title":"J Am Soc Mass Spectrom"},{"key":"8_CR30","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.bbrc.2022.04.004","volume":"609","author":"A Islam","year":"2022","unstructured":"Islam A, Takeyama E, Nabi MM et al (2022) Stress upregulates 2-arachidonoylglycerol levels in the hypothalamus, midbrain, and hindbrain, and it is sustained by green nut oil supplementation in SAMP8 mice revealed by DESI-MSI. Biochem Biophys Res Commun 609:9\u201314","journal-title":"Biochem Biophys Res Commun"},{"key":"8_CR31","doi-asserted-by":"publisher","first-page":"1355","DOI":"10.1039\/C9JA00118B","volume":"34","author":"P Ag\u00fci-Gonzalez","year":"2019","unstructured":"Ag\u00fci-Gonzalez P, J\u00e4hne S, Phan NT (2019) SIMS imaging in neurobiology and cell biology. J Anal At Spectrom 34:1355\u20131368","journal-title":"J Anal At Spectrom"},{"key":"8_CR32","doi-asserted-by":"publisher","first-page":"694","DOI":"10.1016\/j.jpha.2023.04.003","volume":"13","author":"Y Fangma","year":"2023","unstructured":"Fangma Y, Liu M, Liao J et al (2023) Dissecting the brain with spatially resolved multi-omics. J Pharm Anal 13:694\u2013710","journal-title":"J Pharm Anal"},{"key":"8_CR33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s40580-018-0157-y","volume":"5","author":"S Yoon","year":"2018","unstructured":"Yoon S, Lee TG (2018) Biological tissue sample preparation for time-of-flight secondary ion mass spectrometry (ToF\u2013SIMS) imaging. Nano Converg 5:1\u201313","journal-title":"Nano Converg"},{"key":"8_CR34","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1021\/jasms.1c00208","volume":"33","author":"JN Jens","year":"2022","unstructured":"Jens JN, Breiner DJ, Phelan VV (2022) Spray-based application of matrix to agar-based microbial samples for reproducible sample adherence in MALDI MSI. J Am Soc Mass Spectrom 33:731\u2013734","journal-title":"J Am Soc Mass Spectrom"},{"key":"8_CR35","doi-asserted-by":"publisher","first-page":"1314","DOI":"10.3390\/ph15111314","volume":"15","author":"A Islam","year":"2022","unstructured":"Islam A, Sakamoto T, Zhai Q et al (2022) Application of AP-MALDI imaging mass microscope for the rapid mapping of imipramine, chloroquine, and their metabolites in the kidney and brain of wild-type mice. Pharmaceuticals 15:1314","journal-title":"Pharmaceuticals"},{"key":"8_CR36","doi-asserted-by":"publisher","first-page":"976","DOI":"10.1016\/j.bbalip.2011.05.007","volume":"1811","author":"MK Passarelli","year":"2011","unstructured":"Passarelli MK, Winograd N (2011) Lipid imaging with time-of-flight secondary ion mass spectrometry (ToF-SIMS). Biochim Biophys Acta-Mol Cell Biol Lipids 1811:976\u2013990","journal-title":"Biochim Biophys Acta-Mol Cell Biol Lipids"},{"key":"8_CR37","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/S1872-2040(17)61063-6","volume":"46","author":"L Hao-Wen","year":"2018","unstructured":"Hao-Wen L, Xin H, Yi-Tao L (2018) Metal\/matrix enhanced time-of-flight secondary ion mass spectrometry for single cell lipids analysis. Chin J Anal Chem 46:61\u201366","journal-title":"Chin J Anal Chem"},{"key":"8_CR38","doi-asserted-by":"publisher","first-page":"1776","DOI":"10.1194\/jlr.M900047-JLR200","volume":"50","author":"Y Sugiura","year":"2009","unstructured":"Sugiura Y, Konishi Y, Zaima N et al (2009) Visualization of the cell-selective distribution of PUFA-containing phosphatidylcholines in mouse brain by imaging mass spectrometry [S]. J Lipid Res 50:1776\u20131788","journal-title":"J Lipid Res"},{"key":"8_CR39","doi-asserted-by":"publisher","first-page":"1090","DOI":"10.1021\/ac3029618","volume":"85","author":"DA Pirman","year":"2013","unstructured":"Pirman DA, Kiss A, Heeren RM et al (2013) Identifying tissue-specific signal variation in MALDI mass spectrometric imaging by use of an internal standard. Anal Chem 85:1090\u20131096","journal-title":"Anal Chem"},{"key":"8_CR40","doi-asserted-by":"publisher","first-page":"A0025","DOI":"10.5702\/massspectrometry.A0025","volume":"3","author":"N Takai","year":"2014","unstructured":"Takai N, Tanaka Y, Saji H (2014) Quantification of small molecule drugs in biological tissue sections by imaging mass spectrometry using surrogate tissue-based calibration standards. Mass Spectrom 3:A0025","journal-title":"Mass Spectrom"},{"key":"8_CR41","doi-asserted-by":"publisher","first-page":"10584","DOI":"10.1021\/acs.analchem.1c01792","volume":"93","author":"T Boskamp","year":"2021","unstructured":"Boskamp T, Casadonte R, Hauberg-Lotte L et al (2021) Cross-normalization of MALDI mass spectrometry imaging data improves site-to-site reproducibility. Anal Chem 93:10584\u201310592","journal-title":"Anal Chem"},{"key":"8_CR42","doi-asserted-by":"publisher","first-page":"2137","DOI":"10.1039\/D3CC05988J","volume":"60","author":"JH Holbrook","year":"2024","unstructured":"Holbrook JH, Kemper GE, Hummon AB (2024) Quantitative mass spectrometry imaging: therapeutics & biomolecules. Chem Commun 60:2137\u20132151","journal-title":"Chem Commun"}],"container-title":["Methods in Molecular Biology","Gangliosides"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5288-6_8","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T04:03:25Z","timestamp":1787112205000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5288-6_8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071652879","9781071652886"],"references-count":42,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5288-6_8","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"20 August 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}},{"value":"The authors have no conflicts of interest to declare that are relevant to the content of this chapter.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}}]}}