{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:59:20Z","timestamp":1790812760641,"version":"4.1.0"},"publisher-location":"New York, NY","reference-count":24,"publisher":"Springer US","isbn-type":[{"value":"9781071652916","type":"print"},{"value":"9781071652923","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5292-3_5","type":"book-chapter","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T22:36:53Z","timestamp":1788302213000},"page":"61-88","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Sperm Proteomics and Phosphoproteomics: High-Throughput Methods Tailored to Gain Insight into Sperm Cell Signaling Pathways"],"prefix":"10.1007","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7256-1403","authenticated-orcid":false,"given":"Elizabeth G.","family":"Bromfield","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan D.","family":"Burke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon P.","family":"Smyth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgia E.","family":"Blackley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jess E.","family":"Mulhall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett","family":"Nixon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean J.","family":"Humphrey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Skerrett-Byrne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,2]]},"reference":[{"issue":"5","key":"5_CR1","doi-asserted-by":"publisher","first-page":"659","DOI":"10.3390\/biology11050659","volume":"11","author":"R Serrano","year":"2022","unstructured":"Serrano R, Garcia-Marin LJ, Bragado MJ (2022) Sperm phosphoproteome: unraveling male infertility. Biology (Basel) 11(5):659. https:\/\/doi.org\/10.3390\/biology11050659","journal-title":"Biology (Basel)"},{"issue":"8","key":"5_CR2","doi-asserted-by":"publisher","first-page":"100810","DOI":"10.1016\/j.mcpro.2024.100810","volume":"23","author":"X Zhang","year":"2024","unstructured":"Zhang X, Tu H, Zhou X, Wang B, Guo Y, Situ C, Qi Y, Li Y, Guo X (2024) Quantitative phosphoproteomic profiling of mouse sperm maturation in epididymis revealed kinases important for sperm motility. Mol Cell Proteomics 23(8):100810. https:\/\/doi.org\/10.1016\/j.mcpro.2024.100810","journal-title":"Mol Cell Proteomics"},{"key":"5_CR3","doi-asserted-by":"publisher","first-page":"110992","DOI":"10.1016\/j.mce.2020.110992","volume":"518","author":"C Baro Graf","year":"2020","unstructured":"Baro Graf C, Ritagliati C, Stival C, Luque GM, Gentile I, Buffone MG, Krapf D (2020) Everything you ever wanted to know about PKA regulation and its involvement in mammalian sperm capacitation. Mol Cell Endocrinol 518:110992. https:\/\/doi.org\/10.1016\/j.mce.2020.110992","journal-title":"Mol Cell Endocrinol"},{"issue":"1","key":"5_CR4","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-023-01080-w","volume":"21","author":"A Mostek-Majewska","year":"2023","unstructured":"Mostek-Majewska A, Majewska A, Janta A, Ciereszko A (2023) New insights into posttranslational modifications of proteins during bull sperm capacitation. Cell Commun Signal 21(1):72. https:\/\/doi.org\/10.1186\/s12964-023-01080-w","journal-title":"Cell Commun Signal"},{"key":"5_CR5","doi-asserted-by":"publisher","first-page":"110955","DOI":"10.1016\/j.mce.2020.110955","volume":"517","author":"B Nixon","year":"2020","unstructured":"Nixon B, Cafe SL, Eamens AL, De Iuliis GN, Bromfield EG, Martin JH, Skerrett-Byrne DA, Dun MD (2020) Molecular insights into the divergence and diversity of post-testicular maturation strategies. Mol Cell Endocrinol 517:110955. https:\/\/doi.org\/10.1016\/j.mce.2020.110955","journal-title":"Mol Cell Endocrinol"},{"key":"5_CR6","doi-asserted-by":"publisher","first-page":"RD24079","DOI":"10.1071\/rd24079","volume":"36","author":"DA Skerrett-Byrne","year":"2024","unstructured":"Skerrett-Byrne DA, Teperino R, Nixon B (2024) ShinySperm: navigating the sperm proteome landscape. Reprod Fertil Dev 36:RD24079. https:\/\/doi.org\/10.1071\/rd24079","journal-title":"Reprod Fertil Dev"},{"issue":"1","key":"5_CR7","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1124\/pharmrev.122.000787","volume":"76","author":"L Nickels","year":"2023","unstructured":"Nickels L, Yan W (2023) Nonhormonal male contraceptive development-strategies for progress. Pharmacol Rev 76(1):37\u201348. https:\/\/doi.org\/10.1124\/pharmrev.122.000787","journal-title":"Pharmacol Rev"},{"key":"5_CR8","doi-asserted-by":"publisher","first-page":"341","DOI":"10.3389\/fcell.2019.00341","volume":"7","author":"S Dey","year":"2019","unstructured":"Dey S, Brothag C, Vijayaraghavan S (2019) Signaling enzymes required for sperm maturation and fertilization in mammals. Front Cell Dev Biol 7:341. https:\/\/doi.org\/10.3389\/fcell.2019.00341","journal-title":"Front Cell Dev Biol"},{"issue":"9","key":"5_CR9","doi-asserted-by":"publisher","first-page":"1897","DOI":"10.1038\/s41596-018-0014-9","volume":"13","author":"SJ Humphrey","year":"2018","unstructured":"Humphrey SJ, Karayel O, James DE, Mann M (2018) High-throughput and high-sensitivity phosphoproteomics with the EasyPhos platform. Nat Protoc 13(9):1897\u20131916. https:\/\/doi.org\/10.1038\/s41596-018-0014-9","journal-title":"Nat Protoc"},{"issue":"7","key":"5_CR10","doi-asserted-by":"publisher","first-page":"111655","DOI":"10.1016\/j.celrep.2022.111655","volume":"41","author":"DA Skerrett-Byrne","year":"2022","unstructured":"Skerrett-Byrne DA, Anderson AL, Bromfield EG, Bernstein IR, Mulhall JE, Schjenken JE, Dun MD, Humphrey SJ, Nixon B (2022) Global profiling of the proteomic changes associated with the post-testicular maturation of mouse spermatozoa. Cell Rep 41(7):111655. https:\/\/doi.org\/10.1016\/j.celrep.2022.111655","journal-title":"Cell Rep"},{"issue":"5","key":"5_CR11","doi-asserted-by":"publisher","first-page":"1038","DOI":"10.1111\/andr.13636","volume":"12","author":"DA Skerrett-Byrne","year":"2024","unstructured":"Skerrett-Byrne DA, Stanger SJ, Trigg NA, Anderson AL, Sipil\u00e4 P, Bernstein IR, Lord T, Schjenken JE, Murray HC, Verrills NM, Dun MD, Pang TY, Nixon B (2024) Phosphoproteomic analysis of the adaption of epididymal epithelial cells to corticosterone challenge. Andrology 12(5):1038\u20131057. https:\/\/doi.org\/10.1111\/andr.13636","journal-title":"Andrology"},{"issue":"7","key":"5_CR12","doi-asserted-by":"publisher","first-page":"e2300253","DOI":"10.1002\/pmic.202300253","volume":"24","author":"JE Mulhall","year":"2024","unstructured":"Mulhall JE, Trigg NA, Bernstein IR, Anderson AL, Murray HC, Sipil\u00e4 P, Lord T, Schjenken JE, Nixon B, Skerrett-Byrne DA (2024) Immortalized mouse caput epididymal epithelial (mECap18) cell line recapitulates the in-vivo environment. Proteomics 24(7):e2300253. https:\/\/doi.org\/10.1002\/pmic.202300253","journal-title":"Proteomics"},{"issue":"9","key":"5_CR13","doi-asserted-by":"publisher","first-page":"e2100227","DOI":"10.1002\/pmic.202100227","volume":"22","author":"SP Smyth","year":"2022","unstructured":"Smyth SP, Nixon B, Anderson AL, Murray HC, Martin JH, MacDougall LA, Robertson SA, Skerrett-Byrne DA, Schjenken JE (2022) Elucidation of the protein composition of mouse seminal vesicle fluid. Proteomics 22(9):e2100227. https:\/\/doi.org\/10.1002\/pmic.202100227","journal-title":"Proteomics"},{"issue":"12","key":"5_CR14","doi-asserted-by":"publisher","first-page":"2301","DOI":"10.1038\/nprot.2016.136","volume":"11","author":"S Tyanova","year":"2016","unstructured":"Tyanova S, Temu T, Cox J (2016) The MaxQuant computational platform for mass spectrometry-based shotgun proteomics. Nat Protoc 11(12):2301\u20132319. https:\/\/doi.org\/10.1038\/nprot.2016.136","journal-title":"Nat Protoc"},{"issue":"9","key":"5_CR15","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1038\/nmeth.3901","volume":"13","author":"S Tyanova","year":"2016","unstructured":"Tyanova S, Temu T, Sinitcyn P, Carlson A, Hein MY, Geiger T, Mann M, Cox J (2016) The Perseus computational platform for comprehensive analysis of (prote)omics data. Nat Methods 13(9):731\u2013740. https:\/\/doi.org\/10.1038\/nmeth.3901","journal-title":"Nat Methods"},{"issue":"11","key":"5_CR16","doi-asserted-by":"publisher","first-page":"2498","DOI":"10.1101\/gr.1239303","volume":"13","author":"P Shannon","year":"2003","unstructured":"Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, Amin N, Schwikowski B, Ideker T (2003) Cytoscape: a software environment for integrated models of biomolecular interaction networks. Genome Res 13(11):2498\u20132504. https:\/\/doi.org\/10.1101\/gr.1239303","journal-title":"Genome Res"},{"issue":"11","key":"5_CR17","doi-asserted-by":"publisher","first-page":"1635","DOI":"10.1093\/bioinformatics\/btaa916","volume":"37","author":"MG Leeming","year":"2021","unstructured":"Leeming MG, O\u2019Callaghan S, Licata L, Iannuccelli M, Lo Surdo P, Micarelli E, Ang CS, Nie S, Varshney S, Ameen S, Cheng HC, Williamson NA (2021) Phosphomatics: interactive interrogation of substrate-kinase networks in global phosphoproteomics datasets. Bioinformatics 37(11):1635\u20131636. https:\/\/doi.org\/10.1093\/bioinformatics\/btaa916","journal-title":"Bioinformatics"},{"issue":"1","key":"5_CR18","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1186\/s12859-016-1433-7","volume":"18","author":"S Eid","year":"2017","unstructured":"Eid S, Turk S, Volkamer A, Rippmann F, Fulle S (2017) KinMap: a web-based tool for interactive navigation through human kinome data. BMC Bioinf 18(1):16. https:\/\/doi.org\/10.1186\/s12859-016-1433-7","journal-title":"BMC Bioinf"},{"key":"5_CR19","doi-asserted-by":"publisher","first-page":"836208","DOI":"10.3389\/fcell.2022.836208","volume":"10","author":"M Zhang","year":"2022","unstructured":"Zhang M, Chiozzi RZ, Skerrett-Byrne DA, Veenendaal T, Klumperman J, Heck AJR, Nixon B, Helms JB, Gadella BM, Bromfield EG (2022) High resolution proteomic analysis of subcellular fractionated boar spermatozoa provides comprehensive insights into perinuclear theca-residing proteins. Front Cell Dev Biol 10:836208. https:\/\/doi.org\/10.3389\/fcell.2022.836208","journal-title":"Front Cell Dev Biol"},{"issue":"5","key":"5_CR20","doi-asserted-by":"publisher","first-page":"1400","DOI":"10.1074\/mcp.M114.044305","volume":"14","author":"R Bruderer","year":"2015","unstructured":"Bruderer R, Bernhardt OM, Gandhi T, Miladinovi\u0107 SM, Cheng LY, Messner S, Ehrenberger T, Zanotelli V, Butscheid Y, Escher C, Vitek O, Rinner O, Reiter L (2015) Extending the limits of quantitative proteome profiling with data-independent acquisition and application to acetaminophen-treated three-dimensional liver microtissues. Mol Cell Proteomics 14(5):1400\u20131410. https:\/\/doi.org\/10.1074\/mcp.M114.044305","journal-title":"Mol Cell Proteomics"},{"issue":"12","key":"5_CR21","doi-asserted-by":"publisher","first-page":"5354","DOI":"10.1021\/pr200611n","volume":"10","author":"T Taus","year":"2011","unstructured":"Taus T, K\u00f6cher T, Pichler P, Paschke C, Schmidt A, Henrich C, Mechtler K (2011) Universal and confident phosphorylation site localization using phosphoRS. J Proteome Res 10(12):5354\u20135362. https:\/\/doi.org\/10.1021\/pr200611n","journal-title":"J Proteome Res"},{"issue":"D1","key":"5_CR22","doi-asserted-by":"publisher","first-page":"D543-d552","DOI":"10.1093\/nar\/gkab1038","volume":"50","author":"Y Perez-Riverol","year":"2022","unstructured":"Perez-Riverol Y, Bai J, Bandla C, Garc\u00eda-Seisdedos D, Hewapathirana S, Kamatchinathan S, Kundu DJ, Prakash A, Frericks-Zipper A, Eisenacher M, Walzer M, Wang S, Brazma A, Vizca\u00edno JA (2022) The PRIDE database resources in 2022: a hub for mass spectrometry-based proteomics evidences. Nucleic Acids Res 50(D1):D543-d552. https:\/\/doi.org\/10.1093\/nar\/gkab1038","journal-title":"Nucleic Acids Res"},{"issue":"8","key":"5_CR23","doi-asserted-by":"publisher","first-page":"1896","DOI":"10.1038\/nprot.2007.261","volume":"2","author":"J Rappsilber","year":"2007","unstructured":"Rappsilber J, Mann M, Ishihama Y (2007) Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips. Nat Protoc 2(8):1896\u20131906. https:\/\/doi.org\/10.1038\/nprot.2007.261","journal-title":"Nat Protoc"},{"issue":"2","key":"5_CR24","doi-asserted-by":"publisher","DOI":"10.1093\/reprod\/xaag012","volume":"171","author":"NA Trigg","year":"2026","unstructured":"Trigg NA, Mulhall JE, Nixon B, Laurent K, Pai S, Smyth SP, Burke ND, Beckers J, Bromfield EG, Karr TL, Lord T, Pleuger C, Schjenken JE, Hrab\u011b de Angelis M, Teperino R, Skerrett-Byrne DA (2026) Curating fertility-proteomic remodelling of sperm during epididymal transit. Reproduction 171(2), xaag012. https:\/\/doi.org\/10.1093\/reprod\/xaag012","journal-title":"Reproduction"}],"container-title":["Methods in Molecular Biology","The Preimplantation Embryo"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5292-3_5","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T22:36:54Z","timestamp":1788302214000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5292-3_5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071652916","9781071652923"],"references-count":24,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5292-3_5","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"2 September 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}