{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T22:23:12Z","timestamp":1787696592331,"version":"build-2784847793"},"publisher-location":"New York, NY","reference-count":22,"publisher":"Springer US","isbn-type":[{"value":"9781071653074","type":"print"},{"value":"9781071653081","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5308-1_13","type":"book-chapter","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T22:03:37Z","timestamp":1787695417000},"page":"201-219","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Implementing In Vitro and In Vivo Approaches to Analyze Lipid\u2013Protein Interactions in Growing Tobacco Pollen Tubes"],"prefix":"10.1007","author":[{"given":"Eli\u0161ka","family":"\u0160krab\u00e1lkov\u00e1","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ond\u0159ej","family":"Novotn\u00fd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u0159emysl","family":"Pejchar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Potock\u00fd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,2]]},"reference":[{"key":"13_CR1","doi-asserted-by":"publisher","first-page":"1587","DOI":"10.1093\/jxb\/erv052","volume":"66","author":"J Sekere\u0161","year":"2015","unstructured":"Sekere\u0161 J, Pleskot R, Pejchar P, \u017d\u00e1rsk\u00fd V, Potock\u00fd M (2015) The song of lipids and proteins: dynamic lipid-protein interfaces in the regulation of plant cell polarity at different scales. J Exp Bot 66:1587\u20131598. https:\/\/doi.org\/10.1093\/jxb\/erv052","journal-title":"J Exp Bot"},{"key":"13_CR2","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiae558","volume":"197","author":"C Fritz","year":"2025","unstructured":"Fritz C, Reimann TM, Adler J, Knab J, Schulmeister S et al (2025) Plasma membrane and cytoplasmic compartmentalization: a dynamic structural framework required for pollen tube tip growth. Plant Physiol 197:kiae558. https:\/\/doi.org\/10.1093\/plphys\/kiae558","journal-title":"Plant Physiol"},{"key":"13_CR3","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1111\/nph.12814","volume":"203","author":"M Potock\u00fd","year":"2014","unstructured":"Potock\u00fd M, Pleskot R, Pejchar P, Vitale N, Kost B et al (2014) Live-cell imaging of phosphatidic acid dynamics in pollen tubes visualized by Spo20p-derived biosensor. New Phytol 203:483\u2013494. https:\/\/doi.org\/10.1111\/nph.12814","journal-title":"New Phytol"},{"key":"13_CR4","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.1028311","volume":"13","author":"N Serrano","year":"2022","unstructured":"Serrano N, Pejchar P, Soukupov\u00e1 H, Hub\u00e1lek M, Potock\u00fd M (2022) Comprehensive analysis of glycerolipid dynamics during tobacco pollen germination and pollen tube growth. Front Plant Sci 13:1028311. https:\/\/doi.org\/10.3389\/fpls.2022.1028311","journal-title":"Front Plant Sci"},{"key":"13_CR5","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-23314-6","volume":"12","author":"K Yperman","year":"2021","unstructured":"Yperman K, Papageorgiou AC, Merceron R, De Munck S, Bloch Y et al (2021) Distinct EH domains of the endocytic TPLATE complex confer lipid and protein binding. Nat Commun 12:3050. https:\/\/doi.org\/10.1038\/s41467-021-23314-6","journal-title":"Nat Commun"},{"key":"13_CR6","doi-asserted-by":"publisher","first-page":"3170","DOI":"10.1105\/tpc.19.00844","volume":"32","author":"Y Zhou","year":"2020","unstructured":"Zhou Y, Yang Y, Niu Y, Fan T, Qian D et al (2020) The tip-localized phosphatidylserine established by Arabidopsis ALA3 is crucial for Rab GTPase-mediated vesicle trafficking and pollen tube growth. Plant Cell 32:3170\u20133187. https:\/\/doi.org\/10.1105\/tpc.19.00844","journal-title":"Plant Cell"},{"key":"13_CR7","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1111\/tpj.14720","volume":"103","author":"P Pejchar","year":"2020","unstructured":"Pejchar P, Sekere\u0161 J, Novotn\u00fd O, \u017d\u00e1rsk\u00fd V, Potock\u00fd M (2020) Functional analysis of phospholipase D\u03b4 family in tobacco pollen tubes. Plant J 103:212\u2013226. https:\/\/doi.org\/10.1111\/tpj.14720","journal-title":"Plant J"},{"key":"13_CR8","doi-asserted-by":"publisher","first-page":"723","DOI":"10.1042\/BST0380723","volume":"38","author":"V \u017d\u00e1rsk\u00fd","year":"2010","unstructured":"\u017d\u00e1rsk\u00fd V, Potock\u00fd M (2010) Recycling domains in plant cell morphogenesis: small GTPase effectors, plasma membrane signaling and the exocyst. Biochem Soc Trans 38:723\u2013728. https:\/\/doi.org\/10.1042\/BST0380723","journal-title":"Biochem Soc Trans"},{"key":"13_CR9","doi-asserted-by":"publisher","first-page":"1659","DOI":"10.1104\/pp.16.01709","volume":"173","author":"J Sekere\u0161","year":"2017","unstructured":"Sekere\u0161 J, Pejchar P, \u0160antr\u016f\u010dek J, Vukasinovic N, \u017d\u00e1rsk\u00fd V et al (2017) Analysis of exocyst subunit EXO70 family reveals distinct membrane polar domains in tobacco pollen tubes. Plant Physiol 173:1659\u20131675. https:\/\/doi.org\/10.1104\/pp.16.01709","journal-title":"Plant Physiol"},{"key":"13_CR10","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2105287118","volume":"118","author":"L Synek","year":"2021","unstructured":"Synek L, Pleskot R, Sekere\u0161 J, Serrano N, Vukasinovic N et al (2021) Plasma membrane phospholipid signature recruits the plant exocyst complex via the EXO70A1 subunit. Proc Natl Acad Sci USA 118:e2105287118. https:\/\/doi.org\/10.1073\/pnas.2105287118","journal-title":"Proc Natl Acad Sci USA"},{"key":"13_CR11","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1126\/stke.2002.129.pl6","volume":"129","author":"S Dowler","year":"2002","unstructured":"Dowler S, Kular G, Alessi DR (2002) Protein lipid overlay assay. Sci STKE 129:6. https:\/\/doi.org\/10.1126\/stke.2002.129.pl6","journal-title":"Sci STKE"},{"key":"13_CR12","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1186\/s13007-020-00578-5","volume":"16","author":"X Han","year":"2020","unstructured":"Han X, Yang Y, Zhao F, Zhang T, Yu X (2020) An improved protein lipid overlay assay for studying lipid\u2013protein interactions. Plant Methods 16:33. https:\/\/doi.org\/10.1186\/s13007-020-00578-5","journal-title":"Plant Methods"},{"key":"13_CR13","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1007\/978-1-62703-401-2_24","volume-title":"Plant lipid signaling protocols","author":"MM Julkowska","year":"2013","unstructured":"Julkowska MM, Rankenberg JM, Testerink C (2013) Liposome-binding assays to assess specificity and affinity of phospholipid\u2013protein interactions. In: Munnik T, Heilmann I (eds) Plant lipid signaling protocols. Humana Press, Totowa, pp 261\u2013271. https:\/\/doi.org\/10.1007\/978-1-62703-401-2_24"},{"key":"13_CR14","doi-asserted-by":"publisher","first-page":"172","DOI":"10.3389\/fmicb.2014.00172","volume":"5","author":"GL Rosano","year":"2014","unstructured":"Rosano GL, Ceccarelli EA (2014) Recombinant protein expression in Escherichia coli: advances and challenges. Front Microbiol 5:172. https:\/\/doi.org\/10.3389\/fmicb.2014.00172","journal-title":"Front Microbiol"},{"key":"13_CR15","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1038\/s43586-021-00046-x","volume":"1","author":"D Garenne","year":"2021","unstructured":"Garenne D, Haines MC, Romantseva EF, Freemont P, Strychalski E et al (2021) Cell-free gene expression. Nat Rev Methods Primers 1:49. https:\/\/doi.org\/10.1038\/s43586-021-00046-x","journal-title":"Nat Rev Methods Primers"},{"key":"13_CR16","doi-asserted-by":"publisher","first-page":"2185","DOI":"10.1111\/nph.17930","volume":"233","author":"P Scholz","year":"2022","unstructured":"Scholz P, Pejchar P, Fernkorn M et al (2022) DIACYLGLYCEROL KINASE 5 regulates polar tip growth of tobacco pollen tubes. New Phytol 233:2185\u20132202. https:\/\/doi.org\/10.1111\/nph.17930","journal-title":"New Phytol"},{"key":"13_CR17","doi-asserted-by":"publisher","unstructured":"\u0160krab\u00e1lkov\u00e1 E, Pejchar P, Potock\u00fd M (2024) Exploring lipid\u2013protein interactions in plant membranes. J Exp Bot:erae199. https:\/\/doi.org\/10.1093\/jxb\/erae199","DOI":"10.1093\/jxb\/erae199"},{"key":"13_CR18","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abe7999","volume":"7","author":"K Yperman","year":"2021","unstructured":"Yperman K, Wang J, Eeckhout D, Winkler J, Vu L et al (2021) Molecular architecture of the endocytic TPLATE complex. Science Adv 7:eabe7999. https:\/\/doi.org\/10.1126\/sciadv.abe7999","journal-title":"Science Adv"},{"key":"13_CR19","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1016\/j.devcel.2018.04.011","volume":"45","author":"MP Platre","year":"2018","unstructured":"Platre MP, Noack LC, Doumane M, Bayle V, Simon MLA et al (2018) A combinatorial lipid code shapes the electrostatic landscape of plant endomembranes. Dev Cell 45:465\u2013480. https:\/\/doi.org\/10.1016\/j.devcel.2018.04.011","journal-title":"Dev Cell"},{"key":"13_CR20","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","volume":"9","author":"J Schindelin","year":"2012","unstructured":"Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M et al (2012) Fiji: an open-source platform for biological-image analysis. Nat Methods 9:676\u2013682. https:\/\/doi.org\/10.1038\/nmeth.2019","journal-title":"Nat Methods"},{"key":"13_CR21","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-015-0393-3","volume":"14","author":"P Dvorak","year":"2015","unstructured":"Dvorak P, Chrast L, Nikel PI, Fedr R, Soucek K et al (2015) Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway. Microb Cell Factories 14:201. https:\/\/doi.org\/10.1186\/s12934-015-0393-3","journal-title":"Microb Cell Factories"},{"key":"13_CR22","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20153803","volume":"20","author":"Z Kub\u00e1tov\u00e1","year":"2019","unstructured":"Kub\u00e1tov\u00e1 Z, Pejchar P, Potock\u00fd M, Sekere\u0161 J, \u017d\u00e1rsk\u00fd V et al (2019) Arabidopsis trichome contains two plasma membrane domains with different lipid compositions which attract distinct EXO70 subunits. Int J Mol Sci 20:3803. https:\/\/doi.org\/10.3390\/ijms20153803","journal-title":"Int J Mol Sci"}],"container-title":["Methods in Molecular Biology","Pollen and Pollen Tube Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5308-1_13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T22:03:37Z","timestamp":1787695417000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5308-1_13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071653074","9781071653081"],"references-count":22,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5308-1_13","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"2 July 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}