{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:17:57Z","timestamp":1788304677095,"version":"build-2803163510"},"publisher-location":"New York, NY","reference-count":61,"publisher":"Springer US","isbn-type":[{"value":"9781071653715","type":"print"},{"value":"9781071653722","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5372-2_17","type":"book-chapter","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:00:30Z","timestamp":1788303630000},"page":"279-299","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterizing Complex Nucleic Acid Interactions of LINE1 ORF1p by Single-Molecule Force Spectroscopy"],"prefix":"10.1007","author":[{"given":"Ben A.","family":"Cashen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Nabuan","family":"Naufer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark C.","family":"Williams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,2]]},"reference":[{"key":"17_CR1","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1016\/S0079-6603(00)64007-2","volume":"64","author":"AV Furano","year":"2000","unstructured":"Furano AV (2000) The biological properties and evolutionary dynamics of mammalian LINE-1 retrotransposons. Prog Nucleic Acid Res Mol Biol 64:255\u2013294","journal-title":"Prog Nucleic Acid Res Mol Biol"},{"issue":"6822","key":"17_CR2","doi-asserted-by":"publisher","first-page":"860","DOI":"10.1038\/35057062","volume":"409","author":"ES Lander","year":"2001","unstructured":"Lander ES, Linton LM, Birren B, Nusbaum C, Zody MC, Baldwin J et al (2001) Initial sequencing and analysis of the human genome. Nature 409(6822):860\u2013921. https:\/\/doi.org\/10.1038\/35057062","journal-title":"Nature"},{"issue":"4","key":"17_CR3","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1056\/NEJMra1510092","volume":"377","author":"HH Kazazian Jr","year":"2017","unstructured":"Kazazian HH Jr, Moran JV (2017) Mobile DNA in health and disease. N Engl J Med 377(4):361\u2013370. https:\/\/doi.org\/10.1056\/NEJMra1510092","journal-title":"N Engl J Med"},{"issue":"4","key":"17_CR4","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1016\/0092-8674(93)90078-5","volume":"72","author":"DD Luan","year":"1993","unstructured":"Luan DD, Korman MH, Jakubczak JL, Eickbush TH (1993) Reverse transcription of R2Bm RNA is primed by a nick at the chromosomal target site: a mechanism for non-LTR retrotransposition. Cell 72(4):595\u2013605","journal-title":"Cell"},{"issue":"2","key":"17_CR5","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1128\/MCB.21.2.467-475.2001","volume":"21","author":"SL Martin","year":"2001","unstructured":"Martin SL, Bushman FD (2001) Nucleic acid chaperone activity of the ORF1 protein from the mouse LINE-1 retrotransposon. Mol Cell Biol 21(2):467\u2013475. https:\/\/doi.org\/10.1128\/MCB.21.2.467-475.2001","journal-title":"Mol Cell Biol"},{"issue":"4","key":"17_CR6","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1038\/74184","volume":"24","author":"C Esnault","year":"2000","unstructured":"Esnault C, Maestre J, Heidmann T (2000) Human LINE retrotransposons generate processed pseudogenes. Nat Genet 24(4):363\u2013367","journal-title":"Nat Genet"},{"issue":"4","key":"17_CR7","doi-asserted-by":"publisher","first-page":"1429","DOI":"10.1128\/MCB.21.4.1429-1439.2001","volume":"21","author":"W Wei","year":"2001","unstructured":"Wei W, Gilbert N, Ooi SL, Lawler JF, Ostertag EM, Kazazian HH et al (2001) Human L1 retrotransposition: cis preference versus trans complementation. Mol Cell Biol 21(4):1429\u20131439","journal-title":"Mol Cell Biol"},{"issue":"12","key":"17_CR8","doi-asserted-by":"publisher","first-page":"2186","DOI":"10.1093\/oxfordjournals.molbev.a003765","volume":"18","author":"S Boissinot","year":"2001","unstructured":"Boissinot S, Furano AV (2001) Adaptive evolution in LINE-1 retrotransposons. Mol Biol Evol 18(12):2186\u20132194","journal-title":"Mol Biol Evol"},{"issue":"25","key":"17_CR9","doi-asserted-by":"publisher","first-page":"9590","DOI":"10.1073\/pnas.0603334103","volume":"103","author":"S Boissinot","year":"2006","unstructured":"Boissinot S, Davis J, Entezam A, Petrov D, Furano AV (2006) Fitness cost of LINE-1 (L1) activity in humans. Proc Natl Acad Sci USA 103(25):9590\u20139594","journal-title":"Proc Natl Acad Sci USA"},{"issue":"5746","key":"17_CR10","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1126\/science.1117196","volume":"310","author":"S Myers","year":"2005","unstructured":"Myers S, Bottolo L, Freeman C, McVean G, Donnelly P (2005) A fine-scale map of recombination rates and hotspots across the human genome. Science 310(5746):321\u2013324. https:\/\/doi.org\/10.1126\/science.1117196","journal-title":"Science"},{"issue":"7004","key":"17_CR11","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1038\/nature02886","volume":"431","author":"D Bourc'his","year":"2004","unstructured":"Bourc'his D, Bestor TH (2004) Meiotic catastrophe and retrotransposon reactivation in male germ cells lacking Dnmt3L. Nature 431(7004):96\u201399","journal-title":"Nature"},{"issue":"2","key":"17_CR12","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1016\/j.devcel.2008.05.015","volume":"15","author":"SFC Soper","year":"2008","unstructured":"Soper SFC, van der Heijden GW, Hardiman TC, Goodheart M, Martin SL, de Boer P et al (2008) Mouse maelstrom, a component of nuage, is essential for spermatogenesis and transposon repression in meiosis. Dev Cell 15(2):285\u2013297","journal-title":"Dev Cell"},{"issue":"4","key":"17_CR13","first-page":"1385","volume":"8","author":"J Skowronski","year":"1988","unstructured":"Skowronski J, Fanning TG, Singer MF (1988) Unit-length line-1 transcripts in human teratocarcinoma cells. Mol Cell Biol 8(4):1385\u20131397","journal-title":"Mol Cell Biol"},{"issue":"6","key":"17_CR14","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1093\/oxfordjournals.molbev.a026372","volume":"17","author":"S Boissinot","year":"2000","unstructured":"Boissinot S, Chevret P, Furano AV (2000) L1 (LINE-1) retrotransposon evolution and amplification in recent human history. Mol Biol Evol 17(6):915\u2013928","journal-title":"Mol Biol Evol"},{"issue":"7","key":"17_CR15","doi-asserted-by":"publisher","first-page":"1221","DOI":"10.1101\/gr.2326704","volume":"14","author":"S Boissinot","year":"2004","unstructured":"Boissinot S, Entezam A, Young L, Munson PJ, Furano AV (2004) The insertional history of an active family of L1 retrotransposons in humans. Genome Res 14(7):1221\u20131231. https:\/\/doi.org\/10.1101\/gr.2326704","journal-title":"Genome Res"},{"issue":"5","key":"17_CR16","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1007\/s00439-005-1321-0","volume":"117","author":"JM Chen","year":"2005","unstructured":"Chen JM, Stenson PD, Cooper DN, Ferec C (2005) A systematic analysis of LINE-1 endonuclease-dependent retrotranspositional events causing human genetic disease. Hum Genet 117(5):411\u2013427. https:\/\/doi.org\/10.1007\/s00439-005-1321-0","journal-title":"Hum Genet"},{"key":"17_CR17","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1146\/annurev-genom-082509-141802","volume":"12","author":"CR Beck","year":"2011","unstructured":"Beck CR, Garcia-Perez JL, Badge RM, Moran JV (2011) LINE-1 elements in structural variation and disease. Annu Rev Genomics Hum Genet 12:187\u2013215. https:\/\/doi.org\/10.1146\/annurev-genom-082509-141802","journal-title":"Annu Rev Genomics Hum Genet"},{"issue":"7","key":"17_CR18","doi-asserted-by":"publisher","first-page":"1171","DOI":"10.1016\/j.cell.2010.05.026","volume":"141","author":"CR Huang","year":"2010","unstructured":"Huang CR, Schneider AM, Lu Y, Niranjan T, Shen P, Robinson MA et al (2010) Mobile interspersed repeats are major structural variants in the human genome. Cell 141(7):1171\u20131182. https:\/\/doi.org\/10.1016\/j.cell.2010.05.026","journal-title":"Cell"},{"issue":"7","key":"17_CR19","doi-asserted-by":"publisher","first-page":"1253","DOI":"10.1016\/j.cell.2010.05.020","volume":"141","author":"RC Iskow","year":"2010","unstructured":"Iskow RC, McCabe MT, Mills RE, Torene S, Pittard WS, Neuwald AF et al (2010) Natural mutagenesis of human genomes by endogenous retrotransposons. Cell 141(7):1253\u20131261. https:\/\/doi.org\/10.1016\/j.cell.2010.05.020","journal-title":"Cell"},{"issue":"5","key":"17_CR20","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1016\/S0092-8674(00)81998-4","volume":"87","author":"JV Moran","year":"1996","unstructured":"Moran JV, Holmes SE, Naas TP, DeBerardinis RJ, Boeke JD, Kazazian HH Jr (1996) High frequency retrotransposition in cultured mammalian cells. Cell 87(5):917\u2013927","journal-title":"Cell"},{"key":"17_CR21","doi-asserted-by":"publisher","unstructured":"Doucet AJ, Hulme AE, Sahinovic E, Kulpa DA, Moldovan JB, Kopera HC et al (2010) Characterization of LINE-1 ribonucleoprotein particles. PLoS Genet 6(10). https:\/\/doi.org\/10.1371\/journal.pgen.1001150","DOI":"10.1371\/journal.pgen.1001150"},{"issue":"7","key":"17_CR22","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1038\/nsmb1107","volume":"13","author":"DA Kulpa","year":"2006","unstructured":"Kulpa DA, Moran JV (2006) Cis-preferential LINE-1 reverse transcriptase activity in ribonucleoprotein particles. Nat Struct Mol Biol 13(7):655\u2013660. https:\/\/doi.org\/10.1038\/nsmb1107","journal-title":"Nat Struct Mol Biol"},{"issue":"21","key":"17_CR23","doi-asserted-by":"publisher","first-page":"3237","DOI":"10.1093\/hmg\/ddi354","volume":"14","author":"DA Kulpa","year":"2005","unstructured":"Kulpa DA, Moran JV (2005) Ribonucleoprotein particle formation is necessary but not sufficient for LINE-1 retrotransposition. Hum Mol Genet 14(21):3237\u20133248. https:\/\/doi.org\/10.1093\/hmg\/ddi354","journal-title":"Hum Mol Genet"},{"issue":"9","key":"17_CR24","first-page":"4804","volume":"11","author":"SL Martin","year":"1991","unstructured":"Martin SL (1991) Ribonucleoprotein particles with LINE-1 RNA in mouse embryonal carcinoma cells. Mol Cell Biol 11(9):4804\u20134807","journal-title":"Mol Cell Biol"},{"issue":"5","key":"17_CR25","doi-asserted-by":"publisher","first-page":"905","DOI":"10.1016\/S0092-8674(00)81997-2","volume":"87","author":"Q Feng","year":"1996","unstructured":"Feng Q, Moran JV, Kazazian HH Jr, Boeke JD (1996) Human L1 retrotransposon encodes a conserved endonuclease required for retrotransposition. Cell 87(5):905\u2013916","journal-title":"Cell"},{"issue":"5039","key":"17_CR26","doi-asserted-by":"publisher","first-page":"1808","DOI":"10.1126\/science.1722352","volume":"254","author":"SL Mathias","year":"1991","unstructured":"Mathias SL, Scott AF, Kazazian HHJ, Boeke JD, Gabriel A (1991) Reverse transcriptase encoded by a human transposable element. Science 254(5039):1808\u20131810","journal-title":"Science"},{"key":"17_CR27","first-page":"1111","volume-title":"Mobile DNA II","author":"TH Eickbush","year":"2002","unstructured":"Eickbush TH, Malik HS (2002) Origins and evolution of retrotransposons. In: Craig NL, Craigie R, Gellert M, Lambowitz AM (eds) Mobile DNA II. American Society of Microbiology Press, Washington, DC, pp 1111\u20131144"},{"issue":"2","key":"17_CR28","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1016\/j.jmb.2005.12.063","volume":"357","author":"S Basame","year":"2006","unstructured":"Basame S, Wai-lun Li P, Howard G, Branciforte D, Keller D, Martin SL (2006) Spatial assembly and RNA binding stoichiometry of a LINE-1 protein essential for retrotransposition. J Mol Biol 357(2):351\u2013357. https:\/\/doi.org\/10.1016\/j.jmb.2005.12.063","journal-title":"J Mol Biol"},{"issue":"10","key":"17_CR29","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1001150","volume":"6","author":"AJ Doucet","year":"2010","unstructured":"Doucet AJ, Hulme AE, Sahinovic E, Kulpa DA, Moldovan JB, Kopera HC et al (2010) Characterization of LINE-1 ribonucleoprotein particles. PLoS Genet 6(10):e1001150","journal-title":"PLoS Genet"},{"issue":"9","key":"17_CR30","doi-asserted-by":"publisher","first-page":"1006","DOI":"10.1038\/nsmb.2097","volume":"18","author":"E Khazina","year":"2011","unstructured":"Khazina E, Truffault V, Buttner R, Schmidt S, Coles M, Weichenrieder O (2011) Trimeric structure and flexibility of the L1ORF1 protein in human L1 retrotransposition. Nat Struct Mol Biol 18(9):1006\u20131014. https:\/\/doi.org\/10.1038\/nsmb.2097","journal-title":"Nat Struct Mol Biol"},{"issue":"3","key":"17_CR31","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1073\/pnas.0809964106","volume":"106","author":"E Khazina","year":"2009","unstructured":"Khazina E, Weichenrieder O (2009) Non-LTR retrotransposons encode noncanonical RRM domains in their first open reading frame. Proc Natl Acad Sci USA 106(3):731\u2013736. https:\/\/doi.org\/10.1073\/pnas.0809964106","journal-title":"Proc Natl Acad Sci USA"},{"issue":"24","key":"17_CR32","doi-asserted-by":"publisher","first-page":"13815","DOI":"10.1073\/pnas.2336221100","volume":"100","author":"SL Martin","year":"2003","unstructured":"Martin SL, Branciforte D, Keller D, Bain DL (2003) Trimeric structure for an essential protein in L1 retrotransposition. Proc Natl Acad Sci USA 100(24):13815\u201313820","journal-title":"Proc Natl Acad Sci USA"},{"issue":"34","key":"17_CR33","doi-asserted-by":"publisher","first-page":"24893","DOI":"10.1074\/jbc.M702023200","volume":"282","author":"K Januszyk","year":"2007","unstructured":"Januszyk K, Li PW-l, Villareal V, Branciforte D, Wu H, Xie Y et al (2007) Identification and solution structure of a highly conserved C-terminal domain within ORF1p required for retrotransposition of long interspersed nuclear element-1. J Biol Chem 282(34):24893\u201324904. https:\/\/doi.org\/10.1074\/jbc.M702023200","journal-title":"J Biol Chem"},{"issue":"13","key":"17_CR34","doi-asserted-by":"publisher","first-page":"5611","DOI":"10.1093\/nar\/gkr171","volume":"39","author":"JD Evans","year":"2011","unstructured":"Evans JD, Peddigari S, Chaurasiya KR, Williams MC, Martin SL (2011) Paired mutations abolish and restore the balanced annealing and melting activities of ORF1p that are required for LINE-1 retrotransposition. Nucleic Acids Res 39(13):5611\u20135621. https:\/\/doi.org\/10.1093\/nar\/gkr171","journal-title":"Nucleic Acids Res"},{"issue":"10","key":"17_CR35","doi-asserted-by":"publisher","first-page":"8112","DOI":"10.1074\/jbc.M210487200","volume":"278","author":"VO Kolosha","year":"2003","unstructured":"Kolosha VO, Martin SL (2003) High-affinity, non-sequence-specific RNA binding by the open reading frame 1 (ORF1) protein from long interspersed nuclear element 1 (LINE-1). J Biol Chem 278(10):8112\u20138117. https:\/\/doi.org\/10.1074\/jbc.M210487200","journal-title":"J Biol Chem"},{"issue":"3","key":"17_CR36","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1016\/j.jmb.2005.03.003","volume":"348","author":"SL Martin","year":"2005","unstructured":"Martin SL, Cruceanu M, Branciforte D, Wai-Lun Li P, Kwok SC, Hodges RS et al (2005) LINE-1 retrotransposition requires the nucleic acid chaperone activity of the ORF1 protein. J Mol Biol 348(3):549\u2013561. https:\/\/doi.org\/10.1016\/j.jmb.2005.03.003","journal-title":"J Mol Biol"},{"issue":"8","key":"17_CR37","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1016\/s0968-0004(98)01256-0","volume":"23","author":"A Rein","year":"1998","unstructured":"Rein A, Henderson LE, Levin JG (1998) Nucleic-acid-chaperone activity of retroviral nucleocapsid proteins: significance for viral replication. Trends Biochem Sci 23(8):297\u2013301. https:\/\/doi.org\/10.1016\/s0968-0004(98)01256-0","journal-title":"Trends Biochem Sci"},{"key":"17_CR38","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1016\/j.semcdb.2018.03.019","volume":"86","author":"MN Naufer","year":"2019","unstructured":"Naufer MN, Furano AV, Williams MC (2019) Protein-nucleic acid interactions of LINE-1 ORF1p. Semin Cell Dev Biol 86:140\u2013149. https:\/\/doi.org\/10.1016\/j.semcdb.2018.03.019","journal-title":"Semin Cell Dev Biol"},{"issue":"18","key":"17_CR39","doi-asserted-by":"publisher","first-page":"5845","DOI":"10.1093\/nar\/gkn554","volume":"36","author":"SL Martin","year":"2008","unstructured":"Martin SL, Bushman D, Wang F, Li PW, Walker A, Cummiskey J et al (2008) A single amino acid substitution in ORF1 dramatically decreases L1 retrotransposition and provides insight into nucleic acid chaperone activity. Nucleic Acids Res 36(18):5845\u20135854. https:\/\/doi.org\/10.1093\/nar\/gkn554","journal-title":"Nucleic Acids Res"},{"issue":"15","key":"17_CR40","doi-asserted-by":"publisher","first-page":"8690","DOI":"10.1093\/nar\/gkac628","volume":"50","author":"BA Cashen","year":"2022","unstructured":"Cashen BA, Naufer MN, Morse M, Jones CE, Williams MC, Furano AV (2022) The L1-ORF1p coiled coil enables formation of a tightly compacted nucleic acid-bound complex that is associated with retrotransposition. Nucleic Acids Res 50(15):8690\u20138699","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"17_CR41","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1093\/nar\/gkv1342","volume":"44","author":"MN Naufer","year":"2016","unstructured":"Naufer MN, Callahan KE, Cook PR, Perez-Gonzalez CE, Williams MC, Furano AV (2016) L1 retrotransposition requires rapid ORF1p oligomerization, a novel coiled coil-dependent property conserved despite extensive remodeling. Nucleic Acids Res 44(1):281\u2013293. https:\/\/doi.org\/10.1093\/nar\/gkv1342","journal-title":"Nucleic Acids Res"},{"issue":"3","key":"17_CR42","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1016\/j.plrev.2010.06.001","volume":"7","author":"KR Chaurasiya","year":"2010","unstructured":"Chaurasiya KR, Paramanathan T, McCauley MJ, Williams MC (2010) Biophysical characterization of DNA binding from single molecule force measurements. Phys Life Rev 7(3):299\u2013341. https:\/\/doi.org\/10.1016\/j.plrev.2010.06.001","journal-title":"Phys Life Rev"},{"issue":"2","key":"17_CR43","doi-asserted-by":"publisher","first-page":"593","DOI":"10.1093\/nar\/gkj458","volume":"34","author":"M Cruceanu","year":"2006","unstructured":"Cruceanu M, Urbaneja MA, Hixson CV, Johnson DG, Datta SA, Fivash MJ et al (2006) Nucleic acid binding and chaperone properties of HIV-1 gag and nucleocapsid proteins. Nucleic Acids Res 34(2):593\u2013605. https:\/\/doi.org\/10.1093\/nar\/gkj458","journal-title":"Nucleic Acids Res"},{"issue":"22","key":"17_CR44","doi-asserted-by":"publisher","first-page":"14013","DOI":"10.1093\/nar\/gkae1141","volume":"52","author":"BA Cashen","year":"2024","unstructured":"Cashen BA, Naufer MN, Morse M, McCauley MJ, Rouzina I, Jones CE et al (2024) L1-ORF1p nucleoprotein can rapidly assume distinct conformations and simultaneously bind more than one nucleic acid. Nucleic Acids Res 52(22):14013\u201314029","journal-title":"Nucleic Acids Res"},{"issue":"16","key":"17_CR45","doi-asserted-by":"publisher","first-page":"8587","DOI":"10.1093\/nar\/gkad595","volume":"51","author":"BA Cashen","year":"2023","unstructured":"Cashen BA, Morse M, Rouzina I, Karpel RL, Williams MC (2023) Dynamic structure of T4 gene 32 protein filaments facilitates rapid noncooperative protein dissociation. Nucleic Acids Res 51(16):8587\u20138605. https:\/\/doi.org\/10.1093\/nar\/gkad595","journal-title":"Nucleic Acids Res"},{"issue":"9","key":"17_CR46","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2024.168544","volume":"436","author":"BA Cashen","year":"2024","unstructured":"Cashen BA, Morse M, Rouzina I, Karpel RL, Williams MC (2024) C-terminal domain of T4 gene 32 protein enables rapid filament reorganization and dissociation. J Mol Biol 436(9):168544. https:\/\/doi.org\/10.1016\/j.jmb.2024.168544","journal-title":"J Mol Biol"},{"issue":"3","key":"17_CR47","doi-asserted-by":"publisher","first-page":"1532","DOI":"10.1093\/nar\/gkaa1267","volume":"49","author":"MN Naufer","year":"2021","unstructured":"Naufer MN, Morse M, M\u00f6ller GB, McIsaac J, Rouzina I, Beuning PJ et al (2021) Multiprotein E. coli SSB\u2013ssDNA complex shows both stable binding and rapid dissociation due to interprotein interactions. Nucleic Acids Res 49(3):1532\u20131549","journal-title":"Nucleic Acids Res"},{"key":"17_CR48","doi-asserted-by":"publisher","DOI":"10.1017\/qrd.2024.21","volume":"6","author":"M Morse","year":"2025","unstructured":"Morse M, Cashen BA, Rouzina I, Williams MC (2025) Diverse single-stranded nucleic acid binding proteins enable both stable protection and rapid exchange required for biological function. QRB Discov 6:e1","journal-title":"QRB Discov"},{"issue":"2","key":"17_CR49","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1002\/bip.20622","volume":"85","author":"MJ McCauley","year":"2007","unstructured":"McCauley MJ, Williams MC (2007) Mechanisms of DNA binding determined in optical tweezers experiments. Biopolymers 85(2):154\u2013168. https:\/\/doi.org\/10.1002\/bip.20622","journal-title":"Biopolymers"},{"issue":"10","key":"17_CR50","doi-asserted-by":"publisher","first-page":"3859","DOI":"10.1073\/pnas.1213676110","volume":"110","author":"GA King","year":"2013","unstructured":"King GA, Gross P, Bockelmann U, Modesti M, Wuite GJ, Peterman EJ (2013) Revealing the competition between peeled ssDNA, melting bubbles, and S-DNA during DNA overstretching using fluorescence microscopy. Proc Natl Acad Sci USA 110(10):3859\u20133864. https:\/\/doi.org\/10.1073\/pnas.1213676110","journal-title":"Proc Natl Acad Sci USA"},{"issue":"10","key":"17_CR51","doi-asserted-by":"publisher","first-page":"3865","DOI":"10.1073\/pnas.1213740110","volume":"110","author":"X Zhang","year":"2013","unstructured":"Zhang X, Chen H, Le S, Rouzina I, Doyle PS, Yan J (2013) Revealing the competition between peeled ssDNA, melting bubbles, and S-DNA during DNA overstretching by single-molecule calorimetry. Proc Natl Acad Sci USA 110(10):3865\u20133870. https:\/\/doi.org\/10.1073\/pnas.1213740110","journal-title":"Proc Natl Acad Sci USA"},{"issue":"1","key":"17_CR52","doi-asserted-by":"publisher","first-page":"gkae1183","DOI":"10.1093\/nar\/gkae1183","volume":"53","author":"V Sundar Rajan","year":"2025","unstructured":"Sundar Rajan V, Levin S, McCauley MJ, Williams MC, Rouzina I, Wilhelmsson LM et al (2025) Force-induced melting and S-DNA pathways for DNA overstretching exhibit distinct kinetics. Nucleic Acids Res 53(1):gkae1183. https:\/\/doi.org\/10.1093\/nar\/gkae1183","journal-title":"Nucleic Acids Res"},{"issue":"13","key":"17_CR53","doi-asserted-by":"publisher","first-page":"8614","DOI":"10.1073\/pnas.132128999","volume":"99","author":"MC Williams","year":"2002","unstructured":"Williams MC, Gorelick RJ, Musier-Forsyth K (2002) Specific zinc-finger architecture required for HIV-1 nucleocapsid protein\u2019s nucleic acid chaperone function. Proc Natl Acad Sci USA 99(13):8614\u20138619. https:\/\/doi.org\/10.1073\/pnas.132128999","journal-title":"Proc Natl Acad Sci USA"},{"issue":"11","key":"17_CR54","doi-asserted-by":"publisher","first-page":"6121","DOI":"10.1073\/pnas.101033198","volume":"98","author":"MC Williams","year":"2001","unstructured":"Williams MC, Rouzina I, Wenner JR, Gorelick RJ, Musier-Forsyth K, Bloomfield VA (2001) Mechanism for nucleic acid chaperone activity of HIV-1 nucleocapsid protein revealed by single molecule stretching. Proc Natl Acad Sci USA 98(11):6121\u20136126. https:\/\/doi.org\/10.1073\/pnas.101033198","journal-title":"Proc Natl Acad Sci USA"},{"issue":"3","key":"17_CR55","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1021\/ar010045k","volume":"35","author":"MC Williams","year":"2002","unstructured":"Williams MC, Rouzina I, Bloomfield VA (2002) Thermodynamics of DNA interactions from single molecule stretching experiments. Acc Chem Res 35(3):159\u2013166","journal-title":"Acc Chem Res"},{"issue":"6","key":"17_CR56","doi-asserted-by":"publisher","first-page":"712","DOI":"10.4161\/rna.7.6.13776","volume":"7","author":"H Wu","year":"2010","unstructured":"Wu H, Rouzina I, Williams MC (2010) Single-molecule stretching studies of RNA chaperones. RNA Biol 7(6):712\u2013723","journal-title":"RNA Biol"},{"issue":"5","key":"17_CR57","doi-asserted-by":"publisher","first-page":"867","DOI":"10.1016\/j.jmb.2006.08.070","volume":"363","author":"M Cruceanu","year":"2006","unstructured":"Cruceanu M, Gorelick RJ, Musier-Forsyth K, Rouzina I, Williams MC (2006) Rapid kinetics of protein-nucleic acid interaction is a major component of HIV-1 nucleocapsid protein's nucleic acid chaperone function. J Mol Biol 363(5):867\u2013877. https:\/\/doi.org\/10.1016\/j.jmb.2006.08.070","journal-title":"J Mol Biol"},{"key":"17_CR58","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/S0079-6603(05)80006-6","volume":"80","author":"JG Levin","year":"2005","unstructured":"Levin JG, Guo J, Rouzina I, Musier-Forsyth K (2005) Nucleic acid chaperone activity of HIV-1 nucleocapsid protein: critical role in reverse transcription and molecular mechanism. Prog Nucleic Acid Res Mol Biol 80:217\u2013286. https:\/\/doi.org\/10.1016\/S0079-6603(05)80006-6","journal-title":"Prog Nucleic Acid Res Mol Biol"},{"issue":"1","key":"17_CR59","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1186\/s12977-016-0322-5","volume":"13","author":"K Post","year":"2016","unstructured":"Post K, Olson ED, Naufer MN, Gorelick RJ, Rouzina I, Williams MC et al (2016) Mechanistic differences between HIV-1 and SIV nucleocapsid proteins and cross-species HIV-1 genomic RNA recognition. Retrovirology 13(1):89. https:\/\/doi.org\/10.1186\/s12977-016-0322-5","journal-title":"Retrovirology"},{"issue":"2","key":"17_CR60","doi-asserted-by":"publisher","first-page":"235","DOI":"10.3390\/v14020235","volume":"14","author":"H Gien","year":"2022","unstructured":"Gien H, Morse M, McCauley MJ, Kitzrow JP, Musier-Forsyth K, Gorelick RJ et al (2022) HIV-1 nucleocapsid protein binds double-stranded DNA in multiple modes to regulate compaction and capsid uncoating. Viruses 14(2):235. https:\/\/doi.org\/10.3390\/v14020235","journal-title":"Viruses"},{"issue":"6","key":"17_CR61","doi-asserted-by":"publisher","first-page":"872","DOI":"10.3390\/v16060872","volume":"16","author":"H Gien","year":"2024","unstructured":"Gien H, Morse M, McCauley MJ, Rouzina I, Gorelick RJ, Williams MC (2024) Cationic residues of the HIV-1 nucleocapsid protein enable DNA condensation to maintain viral core particle stability during reverse transcription. Viruses 16(6):872. https:\/\/doi.org\/10.3390\/v16060872","journal-title":"Viruses"}],"container-title":["Methods in Molecular Biology","RNA Chaperones"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5372-2_17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:00:32Z","timestamp":1788303632000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5372-2_17"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071653715","9781071653722"],"references-count":61,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5372-2_17","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"2 September 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}