{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:18:40Z","timestamp":1788304720674,"version":"build-2803163510"},"publisher-location":"New York, NY","reference-count":35,"publisher":"Springer US","isbn-type":[{"value":"9781071653753","type":"print"},{"value":"9781071653760","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5376-0_22","type":"book-chapter","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:06:59Z","timestamp":1788304019000},"page":"355-369","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prolonging the Lifespan of Neutrophils for Granulocyte Transfusion in Neutropenic Mice with Bacterial Pneumonia"],"prefix":"10.1007","author":[{"given":"Alan Y.","family":"Hsu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuping","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongbo R.","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,2]]},"reference":[{"key":"22_CR1","doi-asserted-by":"publisher","first-page":"113","DOI":"10.3389\/fphys.2018.00113","volume":"9","author":"C Rosales","year":"2018","unstructured":"Rosales C (2018) Neutrophil: a cell with many roles in inflammation or several cell types? Front Physiol 9:113. https:\/\/doi.org\/10.3389\/fphys.2018.00113","journal-title":"Front Physiol"},{"key":"22_CR2","doi-asserted-by":"publisher","first-page":"288","DOI":"10.1002\/ajh.21078","volume":"83","author":"HR Luo","year":"2008","unstructured":"Luo HR, Loison F (2008) Constitutive neutrophil apoptosis: mechanisms and regulation. Am J Hematol 83:288\u2013295. https:\/\/doi.org\/10.1002\/ajh.21078","journal-title":"Am J Hematol"},{"key":"22_CR3","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-22973-9","volume":"12","author":"R Grieshaber-Bouyer","year":"2021","unstructured":"Grieshaber-Bouyer R et al (2021) The neutrotime transcriptional signature defines a single continuum of neutrophils across biological compartments. Nat Commun 12:2856. https:\/\/doi.org\/10.1038\/s41467-021-22973-9","journal-title":"Nat Commun"},{"key":"22_CR4","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1016\/j.it.2019.04.013","volume":"40","author":"A Hidalgo","year":"2019","unstructured":"Hidalgo A, Chilvers ER, Summers C, Koenderman L (2019) The neutrophil life cycle. Trends Immunol 40:584\u2013597. https:\/\/doi.org\/10.1016\/j.it.2019.04.013","journal-title":"Trends Immunol"},{"key":"22_CR5","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1038\/nri3399","volume":"13","author":"E Kolaczkowska","year":"2013","unstructured":"Kolaczkowska E, Kubes P (2013) Neutrophil recruitment and function in health and inflammation. Nat Rev Immunol 13:159\u2013175. https:\/\/doi.org\/nri3399. [pii] 10.1038\/nri3399","journal-title":"Nat Rev Immunol"},{"key":"22_CR6","doi-asserted-by":"publisher","unstructured":"Ley K et al (2018) Neutrophils: new insights and open questions. Sci Immunol 3. https:\/\/doi.org\/10.1126\/sciimmunol.aat4579","DOI":"10.1126\/sciimmunol.aat4579"},{"key":"22_CR7","doi-asserted-by":"publisher","first-page":"1377","DOI":"10.1016\/j.immuni.2021.06.006","volume":"54","author":"GL Burn","year":"2021","unstructured":"Burn GL, Foti A, Marsman G, Patel DF, Zychlinsky A (2021) The neutrophil. Immunity 54:1377\u20131391. https:\/\/doi.org\/10.1016\/j.immuni.2021.06.006","journal-title":"Immunity"},{"issue":"Suppl 4","key":"22_CR8","doi-asserted-by":"publisher","first-page":"S240","DOI":"10.1086\/427329","volume":"40","author":"C Viscoli","year":"2005","unstructured":"Viscoli C, Varnier O, Machetti M (2005) Infections in patients with febrile neutropenia: epidemiology, microbiology, and risk stratification. Clin Infect Dis 40(Suppl 4):S240\u2013S245. https:\/\/doi.org\/10.1086\/427329","journal-title":"Clin Infect Dis"},{"key":"22_CR9","doi-asserted-by":"publisher","first-page":"699","DOI":"10.1148\/radiology.210.3.r99mr39699","volume":"210","author":"AN Leung","year":"1999","unstructured":"Leung AN et al (1999) Pulmonary infections after bone marrow transplantation: clinical and radiographic findings. Radiology 210:699\u2013710. https:\/\/doi.org\/10.1148\/radiology.210.3.r99mr39699","journal-title":"Radiology"},{"issue":"Suppl 1","key":"22_CR10","doi-asserted-by":"publisher","first-page":"S18","DOI":"10.1038\/sj.bjc.6605272","volume":"101","author":"D Cameron","year":"2009","unstructured":"Cameron D (2009) Management of chemotherapy-associated febrile neutropenia. Br J Cancer 101(Suppl 1):S18\u2013S22. https:\/\/doi.org\/10.1038\/sj.bjc.6605272","journal-title":"Br J Cancer"},{"key":"22_CR11","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2022.102427","volume":"109","author":"DW Blayney","year":"2022","unstructured":"Blayney DW, Schwartzberg L (2022) Chemotherapy-induced neutropenia and emerging agents for prevention and treatment: a review. Cancer Treat Rev 109:102427. https:\/\/doi.org\/10.1016\/j.ctrv.2022.102427","journal-title":"Cancer Treat Rev"},{"key":"22_CR12","doi-asserted-by":"publisher","first-page":"1883","DOI":"10.1182\/blood.V81.7.1883.1883","volume":"81","author":"WI Bensinger","year":"1993","unstructured":"Bensinger WI et al (1993) The effects of daily recombinant human granulocyte colony-stimulating factor administration on normal granulocyte donors undergoing leukapheresis. Blood 81:1883\u20131888","journal-title":"Blood"},{"key":"22_CR13","doi-asserted-by":"publisher","first-page":"1256","DOI":"10.1016\/j.jcyt.2017.08.012","volume":"19","author":"J Gea-Banacloche","year":"2017","unstructured":"Gea-Banacloche J (2017) Granulocyte transfusions: a concise review for practitioners. Cytotherapy 19:1256\u20131269. https:\/\/doi.org\/10.1016\/j.jcyt.2017.08.012","journal-title":"Cytotherapy"},{"key":"22_CR14","first-page":"227","volume":"13","author":"RB Epstein","year":"1977","unstructured":"Epstein RB, Zander AR (1977) Granulocyte transfusions in leukopenic dogs. Prog Clin Biol Res 13:227\u2013241","journal-title":"Prog Clin Biol Res"},{"key":"22_CR15","doi-asserted-by":"publisher","first-page":"869","DOI":"10.1182\/blood.V47.5.869.869","volume":"47","author":"DC Dale","year":"1976","unstructured":"Dale DC, Reynolds HY, Pennington JE, Elin RJ, Herzig GP (1976) Experimental pseudomonas pneumonia in leukopenic dogs: comparison of therapy with antibiotics and granulocyte transfusions. Blood 47:869\u2013876","journal-title":"Blood"},{"key":"22_CR16","doi-asserted-by":"publisher","first-page":"6702","DOI":"10.1182\/blood-2010-09-309864","volume":"117","author":"Y Li","year":"2011","unstructured":"Li Y et al (2011) Pretreatment with phosphatase and tensin homolog deleted on chromosome 10 (PTEN) inhibitor SF1670 augments the efficacy of granulocyte transfusion in a clinically relevant mouse model. Blood 117:6702\u20136713. https:\/\/doi.org\/10.1182\/blood-2010-09-309864","journal-title":"Blood"},{"key":"22_CR17","doi-asserted-by":"publisher","first-page":"2819","DOI":"10.1182\/blood.V88.8.2819.bloodjournal8882819","volume":"88","author":"P Anderlini","year":"1996","unstructured":"Anderlini P, Przepiorka D, Champlin R, Korbling M (1996) Biologic and clinical effects of granulocyte colony-stimulating factor in normal individuals. Blood 88:2819\u20132825","journal-title":"Blood"},{"key":"22_CR18","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1097\/00062752-200105000-00006","volume":"8","author":"K Hubel","year":"2001","unstructured":"Hubel K, Dale DC, Liles WC (2001) Granulocyte transfusion therapy: update on potential clinical applications. Curr Opin Hematol 8:161\u2013164. https:\/\/doi.org\/10.1097\/00062752-200105000-00006","journal-title":"Curr Opin Hematol"},{"key":"22_CR19","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1053\/j.seminhematol.2006.09.015","volume":"44","author":"TH Price","year":"2007","unstructured":"Price TH (2007) Granulocyte transfusion: current status. Semin Hematol 44:15\u201323. https:\/\/doi.org\/10.1053\/j.seminhematol.2006.09.015","journal-title":"Semin Hematol"},{"key":"22_CR20","doi-asserted-by":"publisher","first-page":"701","DOI":"10.1056\/NEJM197703312961301","volume":"296","author":"RH Herzig","year":"1977","unstructured":"Herzig RH, Herzig GP, Graw RG Jr, Bull MI, Ray KK (1977) Successful granulocyte transfusion therapy for gram-negative septicemia. A prospectively randomized controlled study. N Engl J Med 296:701\u2013705. https:\/\/doi.org\/10.1056\/NEJM197703312961301","journal-title":"N Engl J Med"},{"key":"22_CR21","doi-asserted-by":"publisher","first-page":"548","DOI":"10.1016\/0002-9343(77)90200-5","volume":"63","author":"WR Vogler","year":"1977","unstructured":"Vogler WR, Winton EF (1977) A controlled study of the efficacy of granulocyte transfusions in patients with neutropenia. Am J Med 63:548\u2013555. https:\/\/doi.org\/10.1016\/0002-9343(77)90200-5","journal-title":"Am J Med"},{"key":"22_CR22","doi-asserted-by":"publisher","first-page":"3431","DOI":"10.1182\/blood-2016-03-700336","volume":"127","author":"J Lahoz-Beneytez","year":"2016","unstructured":"Lahoz-Beneytez J et al (2016) Human neutrophil kinetics: modeling of stable isotope labeling data supports short blood neutrophil half-lives. Blood 127:3431\u20133438. https:\/\/doi.org\/10.1182\/blood-2016-03-700336","journal-title":"Blood"},{"key":"22_CR23","doi-asserted-by":"publisher","unstructured":"Fan Y et al (2021) Targeting multiple cell death pathways extends the shelf life and preserves the function of human and mouse neutrophils for transfusion. Sci Transl Med 13. https:\/\/doi.org\/10.1126\/scitranslmed.abb1069","DOI":"10.1126\/scitranslmed.abb1069"},{"key":"22_CR24","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1023\/a:1024116916932","volume":"8","author":"TM Caserta","year":"2003","unstructured":"Caserta TM, Smith AN, Gultice AD, Reedy MA, Brown TL (2003) Q-VD-OPh, a broad spectrum caspase inhibitor with potent antiapoptotic properties. Apoptosis 8:345\u2013352. https:\/\/doi.org\/10.1023\/a:1024116916932","journal-title":"Apoptosis"},{"key":"22_CR25","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1038\/s41419-017-0058-8","volume":"9","author":"X Wang","year":"2018","unstructured":"Wang X, Yousefi S, Simon HU (2018) Necroptosis and neutrophil-associated disorders. Cell Death Dis 9:111. https:\/\/doi.org\/10.1038\/s41419-017-0058-8","journal-title":"Cell Death Dis"},{"key":"22_CR26","doi-asserted-by":"publisher","first-page":"4445","DOI":"10.1172\/JCI76246","volume":"124","author":"F Loison","year":"2014","unstructured":"Loison F et al (2014) Proteinase 3-dependent caspase-3 cleavage modulates neutrophil death and inflammation. J Clin Invest 124:4445\u20134458. https:\/\/doi.org\/10.1172\/JCI76246","journal-title":"J Clin Invest"},{"key":"22_CR27","doi-asserted-by":"publisher","first-page":"2924","DOI":"10.1016\/j.celrep.2018.02.067","volume":"22","author":"H Kambara","year":"2018","unstructured":"Kambara H et al (2018) Gasdermin D exerts anti-inflammatory effects by promoting neutrophil death. Cell Rep 22:2924\u20132936. https:\/\/doi.org\/10.1016\/j.celrep.2018.02.067","journal-title":"Cell Rep"},{"key":"22_CR28","doi-asserted-by":"publisher","first-page":"2950","DOI":"10.1073\/pnas.0912717107","volume":"107","author":"Y Xu","year":"2010","unstructured":"Xu Y, Loison F, Luo HR (2010) Neutrophil spontaneous death is mediated by down-regulation of autocrine signaling through GPCR, PI3Kgamma, ROS, and actin. Proc Natl Acad Sci USA 107:2950\u20132955. https:\/\/doi.org\/10.1073\/pnas.0912717107","journal-title":"Proc Natl Acad Sci USA"},{"key":"22_CR29","doi-asserted-by":"publisher","first-page":"2046","DOI":"10.1182\/blood-2008-04-149575","volume":"112","author":"BJ van Raam","year":"2008","unstructured":"van Raam BJ, Drewniak A, Groenewold V, van den Berg TK, Kuijpers TW (2008) Granulocyte colony-stimulating factor delays neutrophil apoptosis by inhibition of calpains upstream of caspase-3. Blood 112:2046\u20132054. https:\/\/doi.org\/10.1182\/blood-2008-04-149575","journal-title":"Blood"},{"key":"22_CR30","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-6-55","volume":"6","author":"AF Zuluaga","year":"2006","unstructured":"Zuluaga AF et al (2006) Neutropenia induced in outbred mice by a simplified low-dose cyclophosphamide regimen: characterization and applicability to diverse experimental models of infectious diseases. BMC Infect Dis 6:55. https:\/\/doi.org\/10.1186\/1471-2334-6-55","journal-title":"BMC Infect Dis"},{"key":"22_CR31","doi-asserted-by":"publisher","unstructured":"Fan Y et al (2023) Neutrophil lifespan extension with CLON-G and an in vitro spontaneous death assay. J Vis Exp. https:\/\/doi.org\/10.3791\/65132","DOI":"10.3791\/65132"},{"key":"22_CR32","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16596-9","volume":"11","author":"G Boivin","year":"2020","unstructured":"Boivin G et al (2020) Durable and controlled depletion of neutrophils in mice. Nat Commun 11:2762. https:\/\/doi.org\/10.1038\/s41467-020-16596-9","journal-title":"Nat Commun"},{"key":"22_CR33","doi-asserted-by":"publisher","unstructured":"Olofsen PA et al (2022) Effective, long-term, neutrophil depletion using a murinized anti-Ly-6G 1A8 antibody. Cells 11. https:\/\/doi.org\/10.3390\/cells11213406","DOI":"10.3390\/cells11213406"},{"key":"22_CR34","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/0022-1759(84)90386-7","volume":"70","author":"MJ Bogman","year":"1984","unstructured":"Bogman MJ, Cornelissen IM, Berden JH, De Jong J, Koene RA (1984) A comparative study of total body irradiation as a method of inducing granulocyte depletion in mice. J Immunol Methods 70:31\u201338. https:\/\/doi.org\/10.1016\/0022-1759(84)90386-7","journal-title":"J Immunol Methods"},{"key":"22_CR35","doi-asserted-by":"publisher","first-page":"1001","DOI":"10.1002\/cyto.a.22995","volume":"89","author":"MN Andersen","year":"2016","unstructured":"Andersen MN, Al-Karradi SN, Kragstrup TW, Hokland M (2016) Elimination of erroneous results in flow cytometry caused by antibody binding to Fc receptors on human monocytes and macrophages. Cytometry A 89:1001\u20131009. https:\/\/doi.org\/10.1002\/cyto.a.22995","journal-title":"Cytometry A"}],"container-title":["Methods in Molecular Biology","Methods in Transfusion Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5376-0_22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:07:00Z","timestamp":1788304020000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5376-0_22"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071653753","9781071653760"],"references-count":35,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5376-0_22","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"2 September 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}