{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:16:46Z","timestamp":1788304606642,"version":"build-2803163510"},"publisher-location":"New York, NY","reference-count":21,"publisher":"Springer US","isbn-type":[{"value":"9781071653753","type":"print"},{"value":"9781071653760","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5376-0_7","type":"book-chapter","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T22:48:20Z","timestamp":1788302900000},"page":"119-137","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microscopy-Based Methods for Measuring Platelet and Red Blood Cell Phagocytosis"],"prefix":"10.1007","author":[{"given":"Alequis","family":"Pavon Oro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoya","family":"Tawhidi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoya","family":"Hussain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoelys","family":"Cruz-Leal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaima","family":"Tundidor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lazaro","family":"Gil Gonzalez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuexin","family":"Shan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter A. A.","family":"Norris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan H.","family":"Lazarus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,2]]},"reference":[{"issue":"101112","key":"7_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2023.101112","volume":"61","author":"TJ Gonz\u00e1lez-L\u00f3pez","year":"2023","unstructured":"Gonz\u00e1lez-L\u00f3pez TJ, Provan D, B\u00e1rez A et al (2023) Primary and secondary immune thrombocytopenia (ITP): time for a rethink. Blood Rev 61(101112):101112. https:\/\/doi.org\/10.1016\/j.blre.2023.101112","journal-title":"Blood Rev"},{"key":"7_CR2","doi-asserted-by":"publisher","unstructured":"Mulder FVM, Evers D, de Haas M et al (2023) Severe autoimmune hemolytic anemia; epidemiology, clinical management, outcomes and knowledge gaps. Front Immunol 14. https:\/\/doi.org\/10.3389\/fimmu.2023.1228142","DOI":"10.3389\/fimmu.2023.1228142"},{"issue":"1","key":"7_CR3","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1182\/blood.v59.1.23.23","volume":"59","author":"EF van Leeuwen","year":"1982","unstructured":"van Leeuwen EF, van der Ven JT, Engelfriet CP, von dem Borne AE (1982) Specificity of autoantibodies in autoimmune thrombocytopenia. Blood 59(1):23\u201326. https:\/\/doi.org\/10.1182\/blood.v59.1.23.23","journal-title":"Blood"},{"issue":"2","key":"7_CR4","doi-asserted-by":"publisher","first-page":"256","DOI":"10.1111\/j.1365-2141.1991.tb04530.x","volume":"79","author":"V Kiefel","year":"1991","unstructured":"Kiefel V, Santoso S, Kaufmann E, Mueller-Eckhardt C (1991) Autoantibodies against platelet glycoprotein Ib\/IX: a frequent finding in autoimmune thrombocytopenic purpura. Br J Haematol 79(2):256\u2013262. https:\/\/doi.org\/10.1111\/j.1365-2141.1991.tb04530.x","journal-title":"Br J Haematol"},{"issue":"12","key":"7_CR5","doi-asserted-by":"publisher","first-page":"1459","DOI":"10.1038\/s41372-023-01785-3","volume":"43","author":"RD Christensen","year":"2023","unstructured":"Christensen RD, Bahr TM, Ilstrup SJ, Dizon-Townson DS (2023) Alloimmune hemolytic disease of the fetus and newborn: genetics, structure, and function of the commonly involved erythrocyte antigens. J Perinatol 43(12):1459\u20131467. https:\/\/doi.org\/10.1038\/s41372-023-01785-3","journal-title":"J Perinatol"},{"issue":"101038","key":"7_CR6","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2022.101038","volume":"59","author":"W Stam","year":"2023","unstructured":"Stam W, Wachholz GE, de Pereda JM, Kapur R, van der Schoot E, Margadant C (2023) Fetal and neonatal alloimmune thrombocytopenia: current pathophysiological insights and perspectives for future diagnostics and treatment. Blood Rev 59(101038):101038. https:\/\/doi.org\/10.1016\/j.blre.2022.101038","journal-title":"Blood Rev"},{"issue":"1","key":"7_CR7","doi-asserted-by":"publisher","first-page":"250","DOI":"10.3324\/haematol.2020.248385","volume":"106","author":"PAA Norris","year":"2021","unstructured":"Norris PAA, Segel GB, Burack WR et al (2021) Fc\u03b3RI and Fc\u03b3RIII on splenic macrophages mediate phagocytosis of anti-glycoprotein IIb\/IIIa autoantibody-opsonized platelets in immune thrombocytopenia. Haematologica 106(1):250\u2013254. https:\/\/doi.org\/10.3324\/haematol.2020.248385","journal-title":"Haematologica"},{"issue":"12","key":"7_CR8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0278365","volume":"17","author":"L Gil Gonzalez","year":"2022","unstructured":"Gil Gonzalez L, Fernandez-Marrero Y, Norris PAA et al (2022) THP-1 cells transduced with CD16A utilize Fc\u03b3 receptor I and III in the phagocytosis of IgG-sensitized human erythrocytes and platelets. PLoS One 17(12):e0278365. https:\/\/doi.org\/10.1371\/journal.pone.0278365","journal-title":"PLoS One"},{"issue":"10","key":"7_CR9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0109463","volume":"9","author":"KL Armour","year":"2014","unstructured":"Armour KL, Smith CS, Ip NCY et al (2014) Clearance of human IgG1-sensitised red blood cells in vivo in humans relates to the in vitro properties of antibodies from alternative cell lines. PLoS One 9(10):e109463. https:\/\/doi.org\/10.1371\/journal.pone.0109463","journal-title":"PLoS One"},{"issue":"2","key":"7_CR10","doi-asserted-by":"publisher","first-page":"61","DOI":"10.21307\/immunohematology-2023-010","volume":"39","author":"SJT Nance","year":"2023","unstructured":"Nance SJT (2023) The monocyte monolayer assay, an in vitro method for prediction of in vivo survival of transfused incompatible red blood cells: a review. Immunohematology 39(2):61\u201369. https:\/\/doi.org\/10.21307\/immunohematology-2023-010","journal-title":"Immunohematology"},{"issue":"11","key":"7_CR11","doi-asserted-by":"publisher","first-page":"4028","DOI":"10.1182\/blood-2003-11-3929","volume":"103","author":"S Miescher","year":"2004","unstructured":"Miescher S, Spycher MO, Amstutz H et al (2004) A single recombinant anti-RhD IgG prevents RhD immunization: association of RhD-positive red blood cell clearance rate with polymorphisms in the FcgammaRIIA and FcgammaIIIA genes. Blood 103(11):4028\u20134035. https:\/\/doi.org\/10.1182\/blood-2003-11-3929","journal-title":"Blood"},{"key":"7_CR12","doi-asserted-by":"publisher","unstructured":"Boligan KF, Sandhu G, Branch DR (2022) Methods to evaluate the potential clinical significance of antibodies to red blood cells. Curr Protoc 2(8). https:\/\/doi.org\/10.1002\/cpz1.504","DOI":"10.1002\/cpz1.504"},{"issue":"1_Suppl","key":"7_CR13","doi-asserted-by":"publisher","first-page":"86.55","DOI":"10.4049\/jimmunol.204.supp.86.55","volume":"204","author":"MM Dean","year":"2020","unstructured":"Dean MM, Tran TV, Ji Y et al (2020) Development of a semi-automated monocyte monolayer assay (MMA) to determine the clinical significance of red cell alloantibodies. J Immunol 204(1_Suppl):86.55\u201386.55. https:\/\/doi.org\/10.4049\/jimmunol.204.supp.86.55","journal-title":"J Immunol"},{"issue":"8877","key":"7_CR14","doi-asserted-by":"publisher","first-page":"945","DOI":"10.1016\/0140-6736(93)92000-j","volume":"342","author":"M Debr\u00e9","year":"1993","unstructured":"Debr\u00e9 M, Griscelli C, Bonnet MC et al (1993) Infusion of Fc\u03b3 fragments for treatment of children with acute immune thrombocytopenic purpura. Lancet 342(8877):945\u2013949. https:\/\/doi.org\/10.1016\/0140-6736(93)92000-j","journal-title":"Lancet"},{"issue":"2","key":"7_CR15","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/j.tmrv.2007.12.001","volume":"22","author":"AR Crow","year":"2008","unstructured":"Crow AR, Lazarus AH (2008) The mechanisms of action of intravenous immunoglobulin and polyclonal anti-d immunoglobulin in the amelioration of immune thrombocytopenic purpura: what do we really know? Transfus Med Rev 22(2):103\u2013116. https:\/\/doi.org\/10.1016\/j.tmrv.2007.12.001","journal-title":"Transfus Med Rev"},{"issue":"26","key":"7_CR16","doi-asserted-by":"publisher","first-page":"2420","DOI":"10.1182\/blood.2019003646","volume":"135","author":"R Khan","year":"2020","unstructured":"Khan R, Menard M, Jen CC, Chen X, Norris PAA, Lazarus AH (2020) Inhibition of platelet phagocytosis as an in vitro predictor for therapeutic potential of RBC antibodies in murine ITP. Blood 135(26):2420\u20132424. https:\/\/doi.org\/10.1182\/blood.2019003646","journal-title":"Blood"},{"issue":"7","key":"7_CR17","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1038\/nmeth.2089","volume":"9","author":"CA Schneider","year":"2012","unstructured":"Schneider CA, Rasband WS, Eliceiri KW (2012) NIH Image to ImageJ: 25 years of image analysis. Nat Methods 9(7):671\u2013675. https:\/\/doi.org\/10.1038\/nmeth.2089","journal-title":"Nat Methods"},{"issue":"7","key":"7_CR18","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","volume":"9","author":"J Schindelin","year":"2012","unstructured":"Schindelin J, Arganda-Carreras I, Frise E et al (2012) Fiji: an open-source platform for biological-image analysis. Nat Methods 9(7):676\u2013682. https:\/\/doi.org\/10.1038\/nmeth.2019","journal-title":"Nat Methods"},{"key":"7_CR19","doi-asserted-by":"publisher","unstructured":"Phuangbubpha P, Thara S, Sriboonaied P, Saetan P, Tumnoi W, Charoenpanich A (2023) Optimizing THP-1 macrophage culture for an immune-responsive human intestinal model. Cells 12(10). https:\/\/doi.org\/10.3390\/cells12101427","DOI":"10.3390\/cells12101427"},{"issue":"1","key":"7_CR20","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.23024","volume":"34","author":"M Zhang","year":"2020","unstructured":"Zhang M, Gu L, Zheng P et al (2020) Improvement of cell counting method for Neubauer counting chamber. J Clin Lab Anal 34(1):e23024. https:\/\/doi.org\/10.1002\/jcla.23024","journal-title":"J Clin Lab Anal"},{"key":"7_CR21","unstructured":"EMS. Technical data sheets neubauer haemocytometry. Electron Microscopy Sciences. Web cite. https:\/\/www.emsdiasum.com\/docs\/technical\/datasheet\/68052-14. Accessed July 7, 2024"}],"container-title":["Methods in Molecular Biology","Methods in Transfusion Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5376-0_7","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T22:48:21Z","timestamp":1788302901000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5376-0_7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071653753","9781071653760"],"references-count":21,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5376-0_7","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"2 September 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}