{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T04:51:34Z","timestamp":1791953494701,"version":"4.3.4"},"publisher-location":"New York, NY","reference-count":44,"publisher":"Springer US","isbn-type":[{"value":"9781071653951","type":"print"},{"value":"9781071653968","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5396-8_16","type":"book-chapter","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T04:39:57Z","timestamp":1791952797000},"page":"201-223","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Quantification of Mitochondrial Oxidative Phosphorylation in Skeletal Muscle"],"prefix":"10.1007","author":[{"given":"Aditya N.","family":"Brahmbhatt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher G. R.","family":"Perry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,13]]},"reference":[{"key":"16_CR1","doi-asserted-by":"crossref","unstructured":"Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Drug-induced mitochondrial dysfunction, pp 327\u2013352","DOI":"10.1002\/9780470372531.ch12"},{"issue":"4","key":"16_CR2","doi-asserted-by":"publisher","first-page":"101140","DOI":"10.1016\/j.jbc.2021.101140","volume":"297","author":"CA Schmidt","year":"2021","unstructured":"Schmidt CA, Fisher-Wellman KH, Neufer PD (2021) From OCR and ECAR to energy: perspectives on the design and interpretation of bioenergetics studies. J Biol Chem 297(4):101140","journal-title":"J Biol Chem"},{"key":"16_CR3","unstructured":"Gnaiger E (2007) Isolated mitochondria or permeabilized tissues and cells. 11(983):1\u20135"},{"key":"16_CR4","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1007\/978-1-61779-382-0_3","volume":"810","author":"D Pesta","year":"2012","unstructured":"Pesta D, Gnaiger E (2012) High-resolution respirometry: OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25\u201358","journal-title":"Methods Mol Biol"},{"issue":"3","key":"16_CR5","doi-asserted-by":"publisher","first-page":"e18317","DOI":"10.1371\/journal.pone.0018317","volume":"6","author":"M Picard","year":"2011","unstructured":"Picard M, Taivassalo T, Ritchie D, Wright KJ, Thomas MM, Romestaing C et al (2011) Mitochondrial structure and function are disrupted by standard isolation methods. PLoS One 6(3):e18317","journal-title":"PLoS One"},{"issue":"21","key":"16_CR6","doi-asserted-by":"publisher","first-page":"7836","DOI":"10.1073\/pnas.85.21.7836","volume":"85","author":"JW Pan","year":"1988","unstructured":"Pan JW, Hamm JR, Rothman DL, Shulman RG (1988) Intracellular pH in human skeletal muscle by 1H NMR. Proc Natl Acad Sci U S A 85(21):7836\u20137839","journal-title":"Proc Natl Acad Sci U S A"},{"key":"16_CR7","doi-asserted-by":"crossref","unstructured":"Chen YW, Liu HQ, Wu Q, Huang YH, Tung YY, Lin MH et al (2020) pH mapping of skeletal muscle by chemical exchange saturation transfer (CEST) imaging. Cells 9(12):2610","DOI":"10.3390\/cells9122610"},{"issue":"6","key":"16_CR8","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1007\/BF02388630","volume":"59","author":"P Allsop","year":"1990","unstructured":"Allsop P, Cheetham M, Brooks S, Hall GM, Williams C (1990) Continuous intramuscular pH measurement during the recovery from brief, maximal exercise in man. Eur J Appl Physiol Occup Physiol 59(6):465\u2013470","journal-title":"Eur J Appl Physiol Occup Physiol"},{"issue":"Pt 3","key":"16_CR9","doi-asserted-by":"publisher","first-page":"993","DOI":"10.1113\/jphysiol.2001.012954","volume":"537","author":"D Street","year":"2001","unstructured":"Street D, Bangsbo J, Juel C (2001) Interstitial pH in human skeletal muscle during and after dynamic graded exercise. J Physiol 537(Pt 3):993\u2013998","journal-title":"J Physiol"},{"issue":"2","key":"16_CR10","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1042\/BJ20110366","volume":"437","author":"CG Perry","year":"2011","unstructured":"Perry CG, Kane DA, Lin CT, Kozy R, Cathey BL, Lark DS et al (2011) Inhibiting myosin-ATPase reveals a dynamic range of mitochondrial respiratory control in skeletal muscle. Biochem J 437(2):215\u2013222","journal-title":"Biochem J"},{"issue":"3","key":"16_CR11","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1016\/S0006-3495(83)84449-X","volume":"41","author":"R Benz","year":"1983","unstructured":"Benz R, McLaughlin S (1983) The molecular mechanism of action of the proton ionophore FCCP (carbonylcyanide p-trifluoromethoxyphenylhydrazone). Biophys J 41(3):381\u2013398","journal-title":"Biophys J"},{"key":"16_CR12","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1016\/j.ab.2017.07.009","volume":"552","author":"LD Zorova","year":"2018","unstructured":"Zorova LD, Popkov VA, Plotnikov EY, Silachev DN, Pevzner IB, Jankauskas SS et al (2018) Mitochondrial membrane potential. Anal Biochem 552:50\u201359","journal-title":"Anal Biochem"},{"issue":"2 Pt 2","key":"16_CR13","first-page":"R190","volume":"248","author":"G Sjogaard","year":"1985","unstructured":"Sjogaard G, Adams RP, Saltin B (1985) Water and ion shifts in skeletal muscle of humans with intense dynamic knee extension. Am J Physiol 248(2 Pt 2):R190\u2013R196","journal-title":"Am J Physiol"},{"issue":"4","key":"16_CR14","doi-asserted-by":"publisher","first-page":"957","DOI":"10.1016\/0300-9629(94)90244-5","volume":"109","author":"R Nagaoka","year":"1994","unstructured":"Nagaoka R, Yamashita S, Mizuno M, Akaike N (1994) Intracellular Na+ and K+ shifts induced by contractile activities of rat skeletal muscles. Comp Biochem Physiol A Physiol 109(4):957\u2013965","journal-title":"Comp Biochem Physiol A Physiol"},{"key":"16_CR15","doi-asserted-by":"crossref","unstructured":"Jong CJ, Sandal P, Schaffer SW (2021) The role of taurine in mitochondria health: more than just an antioxidant. Molecules 26(16):4913","DOI":"10.3390\/molecules26164913"},{"issue":"2","key":"16_CR16","first-page":"57","volume":"13","author":"YJ Xu","year":"2008","unstructured":"Xu YJ, Arneja AS, Tappia PS, Dhalla NS (2008) The potential health benefits of taurine in cardiovascular disease. Exp Clin Cardiol 13(2):57\u201365","journal-title":"Exp Clin Cardiol"},{"issue":"Suppl 1","key":"16_CR17","doi-asserted-by":"publisher","first-page":"S23","DOI":"10.1186\/1423-0127-17-S1-S23","volume":"17","author":"SH Hansen","year":"2010","unstructured":"Hansen SH, Andersen ML, Cornett C, Gradinaru R, Grunnet N (2010) A role for taurine in mitochondrial function. J Biomed Sci 17(Suppl 1):S23","journal-title":"J Biomed Sci"},{"key":"16_CR18","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1007\/978-1-4939-7831-1_8","volume":"1782","author":"T Komlodi","year":"2018","unstructured":"Komlodi T, Sobotka O, Krumschnabel G, Bezuidenhout N, Hiller E, Doerrier C et al (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137\u201355.","journal-title":"Methods Mol Biol"},{"issue":"4","key":"16_CR19","doi-asserted-by":"publisher","first-page":"1041","DOI":"10.2337\/db12-1219","volume":"62","author":"CG Perry","year":"2013","unstructured":"Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62(4):1041\u20131053","journal-title":"Diabetes"},{"issue":"5","key":"16_CR20","doi-asserted-by":"publisher","first-page":"1271","DOI":"10.1007\/s00726-011-0877-3","volume":"40","author":"T Wallimann","year":"2011","unstructured":"Wallimann T, Tokarska-Schlattner M, Schlattner U (2011) The creatine kinase system and pleiotropic effects of creatine. Amino Acids 40(5):1271\u20131296","journal-title":"Amino Acids"},{"key":"16_CR21","doi-asserted-by":"crossref","unstructured":"Kaldis P, Kamp G, Piendl T, Wallimann T (1997) Functions of creatine kinase isoenzymes in spermatozoa. Adv Dev Biol (1992) 5:275-312","DOI":"10.1016\/S1566-3116(08)60040-7"},{"key":"16_CR22","doi-asserted-by":"publisher","first-page":"103319","DOI":"10.1016\/j.redox.2024.103319","volume":"76","author":"MC Hughes","year":"2024","unstructured":"Hughes MC, Ramos SV, Brahmbhatt AN, Turnbull PC, Polidovitch NN, Garibotti MC et al (2024) Mitohormesis during advanced stages of Duchenne muscular dystrophy reveals a redox-sensitive creatine pathway that can be enhanced by the mitochondrial-targeting peptide SBT-20. Redox Biol 76:103319","journal-title":"Redox Biol"},{"issue":"21","key":"16_CR23","doi-asserted-by":"publisher","first-page":"5475","DOI":"10.1113\/jphysiol.2012.234682","volume":"590","author":"CG Perry","year":"2012","unstructured":"Perry CG, Kane DA, Herbst EA, Mukai K, Lark DS, Wright DC et al (2012) Mitochondrial creatine kinase activity and phosphate shuttling are acutely regulated by exercise in human skeletal muscle. J Physiol 590(21):5475\u20135486","journal-title":"J Physiol"},{"issue":"11","key":"16_CR24","doi-asserted-by":"publisher","first-page":"3127","DOI":"10.1113\/JP271259","volume":"594","author":"M Ydfors","year":"2016","unstructured":"Ydfors M, Hughes MC, Laham R, Schlattner U, Norrbom J, Perry CG (2016) Modelling in vivo creatine\/phosphocreatine in vitro reveals divergent adaptations in human muscle mitochondrial respiratory control by ADP after acute and chronic exercise. J Physiol 594(11):3127\u20133140","journal-title":"J Physiol"},{"issue":"3","key":"16_CR25","doi-asserted-by":"publisher","first-page":"643","DOI":"10.1002\/jcsm.12405","volume":"10","author":"MC Hughes","year":"2019","unstructured":"Hughes MC, Ramos SV, Turnbull PC, Rebalka IA, Cao A, Monaco CMF et al (2019) Early myopathy in Duchenne muscular dystrophy is associated with elevated mitochondrial H(2) O(2) emission during impaired oxidative phosphorylation. J Cachexia Sarcopenia Muscle 10(3):643\u2013661","journal-title":"J Cachexia Sarcopenia Muscle"},{"issue":"6","key":"16_CR26","doi-asserted-by":"publisher","first-page":"1112","DOI":"10.1139\/H08-097","volume":"33","author":"CG Perry","year":"2008","unstructured":"Perry CG, Heigenhauser GJ, Bonen A, Spriet LL (2008) High-intensity aerobic interval training increases fat and carbohydrate metabolic capacities in human skeletal muscle. Appl Physiol Nutr Metab 33(6):1112\u20131123","journal-title":"Appl Physiol Nutr Metab"},{"issue":"2","key":"16_CR27","doi-asserted-by":"publisher","first-page":"126","DOI":"10.2174\/157488612802715681","volume":"7","author":"E La Monaca","year":"2012","unstructured":"La Monaca E, Fodale V (2012) Effects of anesthetics on mitochondrial signaling and function. Curr Drug Saf 7(2):126\u2013139","journal-title":"Curr Drug Saf"},{"issue":"6","key":"16_CR28","doi-asserted-by":"publisher","first-page":"4025","DOI":"10.1074\/jbc.M109.065664","volume":"285","author":"Y Zhang","year":"2010","unstructured":"Zhang Y, Dong Y, Wu X, Lu Y, Xu Z, Knapp A et al (2010) The mitochondrial pathway of anesthetic isoflurane-induced apoptosis. J Biol Chem 285(6):4025\u201337.","journal-title":"J Biol Chem"},{"issue":"3","key":"16_CR29","first-page":"31","volume":"2","author":"X Tan","year":"2024","unstructured":"Tan X, Liu R, Dan L, Huang H, Duan C (2024) Effects of anesthetics on mitochondrial quality control: mechanisms and clinical implications. Anesthesiology and Perioperative. Science. 2(3):31","journal-title":"Science."},{"issue":"2","key":"16_CR30","first-page":"401","volume":"68","author":"C Kawasaki","year":"2010","unstructured":"Kawasaki C, Kawasaki T, Ogata M, Sata T, Chaudry IH (2010) Lidocaine enhances apoptosis and suppresses mitochondrial functions of human neutrophil in vitro. J Trauma 68(2):401\u2013408","journal-title":"J Trauma"},{"key":"16_CR31","doi-asserted-by":"publisher","first-page":"360","DOI":"10.3389\/fphys.2015.00360","volume":"6","author":"MC Hughes","year":"2015","unstructured":"Hughes MC, Ramos SV, Turnbull PC, Nejatbakhsh A, Baechler BL, Tahmasebi H et al (2015) Mitochondrial bioenergetics and fiber type assessments in microbiopsy vs. bergstrom percutaneous sampling of human skeletal muscle. Front Physiol 6:360","journal-title":"Front Physiol"},{"issue":"7","key":"16_CR32","doi-asserted-by":"publisher","first-page":"609","DOI":"10.3109\/00365517509095787","volume":"35","author":"J Bergstrom","year":"1975","unstructured":"Bergstrom J (1975) Percutaneous needle biopsy of skeletal muscle in physiological and clinical research. Scand J Clin Lab Invest 35(7):609\u2013616","journal-title":"Scand J Clin Lab Invest"},{"key":"16_CR33","doi-asserted-by":"crossref","unstructured":"Dong H, Tsai SY (2023) Mitochondrial properties in skeletal muscle fiber. Cells 12(17):2183","DOI":"10.3390\/cells12172183"},{"key":"16_CR34","doi-asserted-by":"publisher","first-page":"952","DOI":"10.1038\/196952a0","volume":"196","author":"AD Bangham","year":"1962","unstructured":"Bangham AD, Horne RW, Glauert AM, Dingle JT, Lucy JA (1962) Action of saponin on biological cell membranes. Nature 196:952\u2013955","journal-title":"Nature"},{"key":"16_CR35","doi-asserted-by":"publisher","first-page":"102643","DOI":"10.1016\/j.redox.2023.102643","volume":"61","author":"L Goicoechea","year":"2023","unstructured":"Goicoechea L, Conde de la Rosa L, Torres S, Garcia-Ruiz C, Fernandez-Checa JC (2023) Mitochondrial cholesterol: metabolism and impact on redox biology and disease. Redox Biol 61:102643","journal-title":"Redox Biol"},{"issue":"3","key":"16_CR36","doi-asserted-by":"publisher","first-page":"e13775","DOI":"10.1002\/jcsm.13775","volume":"16","author":"L Zuccarelli","year":"2025","unstructured":"Zuccarelli L, De Martino M, Filippi A, Knapton AE, Thackray BD, Baldassarre G et al (2025) Mitochondrial sensitivity to submaximal [ADP] following bed rest: a novel two-phase approach associated with fibre types. J Cachexia Sarcopenia Muscle 16(3):e13775","journal-title":"J Cachexia Sarcopenia Muscle"},{"issue":"2","key":"16_CR37","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1042\/BJ20110162","volume":"435","author":"MD Brand","year":"2011","unstructured":"Brand MD, Nicholls DG (2011) Assessing mitochondrial dysfunction in cells. Biochem J 435(2):297\u2013312","journal-title":"Biochem J"},{"issue":"6","key":"16_CR38","doi-asserted-by":"publisher","first-page":"965","DOI":"10.1038\/nprot.2008.61","volume":"3","author":"AV Kuznetsov","year":"2008","unstructured":"Kuznetsov AV, Veksler V, Gellerich FN, Saks V, Margreiter R, Kunz WS (2008) Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells. Nat Protoc 3(6):965\u2013976","journal-title":"Nat Protoc"},{"issue":"2","key":"16_CR39","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1002\/(SICI)1097-0185(199706)248:2<214::AID-AR8>3.0.CO;2-S","volume":"248","author":"T Ogata","year":"1997","unstructured":"Ogata T, Yamasaki Y (1997) Ultra-high-resolution scanning electron microscopy of mitochondria and sarcoplasmic reticulum arrangement in human red, white, and intermediate muscle fibers. Anat Rec 248(2):214\u2013223","journal-title":"Anat Rec"},{"key":"16_CR40","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1007\/978-1-4939-7831-1_3","volume":"1782","author":"C Doerrier","year":"2018","unstructured":"Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Meszaros AT, Gnaiger E (2018) High-resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31\u201370","journal-title":"Methods Mol Biol"},{"key":"16_CR41","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1016\/j.ab.2013.10.023","volume":"446","author":"S Larsen","year":"2014","unstructured":"Larsen S, Kraunsoe R, Gram M, Gnaiger E, Helge JW, Dela F (2014) The best approach: homogenization or manual permeabilization of human skeletal muscle fibers for respirometry?. Anal Biochem 446:64\u201368","journal-title":"Anal Biochem"},{"key":"16_CR42","doi-asserted-by":"crossref","unstructured":"Gnaiger E (2003) Oxygen conformance of cellular respiration: a perspective of mitochondrial physiology. In: Hypoxia: through the lifecycle, pp 39\u201355.","DOI":"10.1007\/978-1-4419-8997-0_4"},{"key":"16_CR43","first-page":"1","volume":"18","author":"A Eigentler","year":"2013","unstructured":"Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18:1\u20136","journal-title":"Mitochondr Physiol Network"},{"key":"16_CR44","doi-asserted-by":"crossref","unstructured":"Djafarzadeh S, Jakob SM (2017) High-resolution respirometry to assess mitochondrial function in permeabilized and intact cells. J Vis Exp 120:54985","DOI":"10.3791\/54985"}],"container-title":["Methods in Molecular Biology","Myogenesis and Skeletal Muscle Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5396-8_16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T04:40:05Z","timestamp":1791952805000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5396-8_16"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071653951","9781071653968"],"references-count":44,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5396-8_16","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"13 October 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}