{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T13:03:19Z","timestamp":1789909399647,"version":"4.0.1"},"publisher-location":"New York, NY","reference-count":8,"publisher":"Springer US","isbn-type":[{"value":"9781071654118","type":"print"},{"value":"9781071654125","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2027]]},"DOI":"10.1007\/978-1-0716-5412-5_6","type":"book-chapter","created":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T13:00:13Z","timestamp":1789909213000},"page":"69-80","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Animal-Free Intestinal Epithelial 2D \u201cOrganoid-Sheet\u201d Cultures Using Invasin"],"prefix":"10.1007","author":[{"given":"Joost J.A.P.M.","family":"Wijnakker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Clevers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"6_CR1","doi-asserted-by":"publisher","first-page":"1586","DOI":"10.1016\/j.cell.2016.05.082","volume":"165","author":"H Clevers","year":"2016","unstructured":"Clevers H (2016) Modeling development and disease with organoids. Cell 165:1586\u20131597","journal-title":"Cell"},{"key":"6_CR2","doi-asserted-by":"publisher","first-page":"590","DOI":"10.1016\/j.devcel.2016.08.014","volume":"38","author":"K Kretzschmar","year":"2016","unstructured":"Kretzschmar K, Clevers H (2016) Organoids: modeling development and the stem cell niche in a dish. Dev Cell 38:590\u2013600","journal-title":"Dev Cell"},{"key":"6_CR3","doi-asserted-by":"publisher","first-page":"968","DOI":"10.1242\/dev.140566","volume":"144","author":"J Drost","year":"2017","unstructured":"Drost J, Clevers H (2017) Translational applications of adult stem cell-derived organoids. Development 144:968\u2013975","journal-title":"Development"},{"key":"6_CR4","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1038\/s41591-024-03489-3","volume":"31","author":"MMA Verstegen","year":"2025","unstructured":"Verstegen MMA et al (2025) Clinical applications of human organoids. Nat Med 31:409\u2013421","journal-title":"Nat Med"},{"key":"6_CR5","doi-asserted-by":"publisher","first-page":"262","DOI":"10.1038\/nature07935","volume":"459","author":"T Sato","year":"2009","unstructured":"Sato T et al (2009) Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 459:262\u2013265","journal-title":"Nature"},{"key":"6_CR6","doi-asserted-by":"publisher","unstructured":"Yi Miao et al (2020) Next-generation surrogate wnts support organoid growth and deconvolute frizzled pleiotropy in vivo. Cell Stem Cell 27(5):840\u2013851.e6. https:\/\/doi.org\/10.1016\/j.stem.2020.07.020","DOI":"10.1016\/j.stem.2020.07.020"},{"key":"6_CR7","doi-asserted-by":"publisher","first-page":"204","DOI":"10.1084\/jem.145.1.204","volume":"145","author":"RW Orkin","year":"1977","unstructured":"Orkin RW et al (1977) A murine tumor producing a matrix of basement membrane. J Exp Med 145:204\u2013220","journal-title":"J Exp Med"},{"key":"6_CR8","doi-asserted-by":"publisher","first-page":"e2420595121","DOI":"10.1073\/pnas.2420595121","volume":"122","author":"JJAPM Wijnakker","year":"2025","unstructured":"Wijnakker JJAPM et al (2025) Integrin-activating Yersinia protein Invasin sustains long-term expansion of primary epithelial cells as 2D organoid sheets. Proc Natl Acad Sci U S A 122:e2420595121","journal-title":"Proc Natl Acad Sci U S A"}],"container-title":["Methods in Molecular Biology","Intestinal Stem Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5412-5_6","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T13:00:13Z","timestamp":1789909213000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5412-5_6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,21]]},"ISBN":["9781071654118","9781071654125"],"references-count":8,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5412-5_6","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,21]]},"assertion":[{"value":"21 September 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}