{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T07:13:14Z","timestamp":1789283594472,"version":"build-2803163510"},"publisher-location":"New York, NY","reference-count":99,"publisher":"Springer US","isbn-type":[{"value":"9781071654156","type":"print"},{"value":"9781071654163","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5416-3_7","type":"book-chapter","created":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T06:26:26Z","timestamp":1789280786000},"page":"219-257","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microfluidic Platforms for Cryopreservation"],"prefix":"10.1007","author":[{"given":"Gang","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,14]]},"reference":[{"key":"7_CR1","doi-asserted-by":"publisher","first-page":"592242","DOI":"10.3389\/fmed.2020.592242","volume":"7","author":"J Meneghel","year":"2020","unstructured":"Meneghel J, Kilbride P, Morris G (2020) Cryopreservation as a key element in the successful delivery of cell-based therapies\u2014a review. Front Med 7:592242. https:\/\/doi.org\/10.3389\/fmed.2020.592242","journal-title":"Front Med"},{"key":"7_CR2","doi-asserted-by":"publisher","unstructured":"Adamson GD, Dyer S, Zegers-Hochschild F, Chambers G, De Mouzon J, Ishihara O, Kupka M, Baker V, Banker M, Elgindy E, Bai F, Jwa SC (2023) ICMART Preliminary World Repreliminary world report 2019. In: Paper presented at the 39th Annual Meeting of the European Society of Human Reproduction and Embryology (ESHRE), Copenhagen, Denmark, 2023, June 25\u201328. https:\/\/doi.org\/10.1093\/humrep\/dead093.187","DOI":"10.1093\/humrep\/dead093.187"},{"key":"7_CR3","doi-asserted-by":"publisher","first-page":"1313","DOI":"10.1126\/science.adj4220","volume":"380","author":"W Cornwall","year":"2023","unstructured":"Cornwall W (2023) Frozen in time. Science 380:1313\u20131317. https:\/\/doi.org\/10.1126\/science.adj4220","journal-title":"Science"},{"key":"7_CR4","doi-asserted-by":"publisher","first-page":"530","DOI":"10.1038\/nbt.3889","volume":"35","author":"S Giwa","year":"2017","unstructured":"Giwa S, Lewis JK, Alvarez L, Langer R, Roth AE, Church GM, Markmann JF, Sachs DH, Chandraker A, Wertheim JA, Rothblatt M, Boyden ES, Eidbo E, Lee WPA, Pomahac B, Brandacher G, Weinstock DM, Elliott G, Nelson D, Acker JP, Uygun K, Schmalz B, Weegman BP, Tocchio A, Fahy GM, Storey KB, Rubinsky B, Bischof J, Elliott JAW, Woodruff TK, Morris GJ, Demirci U, Brockbank KGM, Woods EJ, Ben RN, Baust JG, Gao D, Fuller B, Rabin Y, Kravitz DC, Taylor MJ, Toner M (2017) The promise of organ and tissue preservation to transform medicine. Nat Biotechnol 35:530\u2013542. https:\/\/doi.org\/10.1038\/nbt.3889","journal-title":"Nat Biotechnol"},{"key":"7_CR5","unstructured":"Food NMoAa (2025) Svalbard Global Seed Vault. Retrieved. https:\/\/www.seedvault.no\/. Accessed 6 Jun 2025"},{"key":"7_CR6","unstructured":"Biobank U (2023) UK Biobank newsletter homepage 2023\/24 [Newsletter]. UK Biobank. https:\/\/www.ukbiobank.ac.uk\/explore-your-participation\/stay-involved\/uk-biobank-newsletter-homepage-2023-24. Accessed 6 Jun 2025"},{"key":"7_CR7","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1085\/jgp.47.2.347","volume":"47","author":"P Mazur","year":"1963","unstructured":"Mazur P (1963) Kinetics of water loss from cells at subzero temperatures and likelihood of intracellular freezing. J Gen Physiol 47:347\u2013369. https:\/\/doi.org\/10.1085\/jgp.47.2.347","journal-title":"J Gen Physiol"},{"issue":"2","key":"7_CR8","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1016\/0014-4827(72)90303-5","volume":"71","author":"P Mazur","year":"1972","unstructured":"Mazur P, Leibo SP, Chu EHY (1972) A two-factor hypothesis of freezing injury: evidence from Chinese hamster tissue-culture cells. Exp Cell Res 71(2):345\u2013355. https:\/\/doi.org\/10.1016\/0014-4827(72)90303-5","journal-title":"Exp Cell Res"},{"key":"7_CR9","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1093\/ilar.41.4.187","volume":"41","author":"D Gao","year":"2000","unstructured":"Gao D, Critser JK (2000) Mechanisms of Cryoinjury in living cells. ILAR J 41:187\u2013196. https:\/\/doi.org\/10.1093\/ilar.41.4.187","journal-title":"ILAR J"},{"key":"7_CR10","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1007\/s40778-022-00212-1","volume":"8","author":"C Cottle","year":"2022","unstructured":"Cottle C, Porter AP, Lipat A, Turner-Lyles C, Nguyen J, Moll G, Chinnadurai R (2022) Impact of cryopreservation and freeze-thawing on therapeutic properties of mesenchymal stromal\/stem cells and other common cellular therapeutics. Curr Stem Cell Rep 8:72\u201392. https:\/\/doi.org\/10.1007\/s40778-022-00212-1","journal-title":"Curr Stem Cell Rep"},{"key":"7_CR11","doi-asserted-by":"publisher","first-page":"104847","DOI":"10.1016\/j.cryobiol.2024.104847","volume":"114","author":"YQ Ding","year":"2024","unstructured":"Ding YQ, Liu S, Liu J, Jin S, Wang J (2024) Cryopreservation with DMSO affects the DNA integrity, apoptosis, cell cycle and function of human bone mesenchymal stem cells. Cryobiology 114:104847. https:\/\/doi.org\/10.1016\/j.cryobiol.2024.104847","journal-title":"Cryobiology"},{"key":"7_CR12","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1016\/j.biotechadv.2017.01.006","volume":"35","author":"G Zhao","year":"2017","unstructured":"Zhao G, Fu JP (2017) Microfluidics for cryopreservation. Biotechnol Adv 35:323\u2013336. https:\/\/doi.org\/10.1016\/j.biotechadv.2017.01.006","journal-title":"Biotechnol Adv"},{"key":"7_CR13","doi-asserted-by":"publisher","first-page":"609","DOI":"10.1002\/btpr.1710","volume":"29","author":"RE Lusianti","year":"2013","unstructured":"Lusianti RE, Benson JD, Acker JP, Higgins AZ (2013) Rapid removal of glycerol from frozen-thawed red blood cells. Biotechnol Prog 29:609\u2013620. https:\/\/doi.org\/10.1002\/btpr.1710","journal-title":"Biotechnol Prog"},{"key":"7_CR14","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1039\/d0tb02426k","volume":"9","author":"LX Shen","year":"2021","unstructured":"Shen LX, Guo X, Ouyang X, Huang Y, Gao D, Zhao G (2021) Fine-tuned dehydration by trehalose enables the cryopreservation of RBCs with unusually low concentrations of glycerol. J Mater Chem B 9:295\u2013306. https:\/\/doi.org\/10.1039\/d0tb02426k","journal-title":"J Mater Chem B"},{"key":"7_CR15","unstructured":"ISO (2018) ISO 20387:2018 biotechnology-biobanking-general requirements for biobanking (2018) Geneva: International Organization for Standardization. https:\/\/www.iso.org\/obp\/ui\/en\/#iso:std:iso:20387:ed-1:v1:en. Accessed 6 June 2025"},{"key":"7_CR16","doi-asserted-by":"publisher","first-page":"1270","DOI":"10.1093\/humrep\/der037","volume":"26","author":"Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology","year":"2011","unstructured":"Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology (2011) The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Hum Reprod 26:1270\u20131283. https:\/\/doi.org\/10.1093\/humrep\/der037","journal-title":"Hum Reprod"},{"key":"7_CR17","doi-asserted-by":"publisher","first-page":"57","DOI":"10.2147\/BSAM.S139376","volume":"5","author":"MC Schiewe","year":"2017","unstructured":"Schiewe MC, Anderson RE (2017) Vitrification: the pioneering past to current trends and perspectives of cryopreserving human embryos, gametes and reproductive tissue. J Biorepository Sci Appl Med 5:57\u201368. https:\/\/doi.org\/10.2147\/BSAM.S139376","journal-title":"J Biorepository Sci Appl Med"},{"key":"7_CR18","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1007\/s10404-008-0265-9","volume":"5","author":"C Mata","year":"2008","unstructured":"Mata C, Longmire EK, McKenna DH, Glass KK, Hubel A (2008) Experimental study of diffusion-based extraction from a cell suspension. Microfluid Nanofluid 5:529\u2013540. https:\/\/doi.org\/10.1007\/s10404-008-0265-9","journal-title":"Microfluid Nanofluid"},{"key":"7_CR19","doi-asserted-by":"publisher","first-page":"703","DOI":"10.1115\/1.2768373","volume":"129","author":"KK Fleming","year":"2007","unstructured":"Fleming KK, Longmire EK, Hubel A (2007) Numerical characterization of diffusion-based extraction in cell-laden flow through a microfluidic channel. J Biomech Eng 129:703\u2013711. https:\/\/doi.org\/10.1115\/1.2768373","journal-title":"J Biomech Eng"},{"key":"7_CR20","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1038\/nature05058","volume":"442","author":"GM Whitesides","year":"2006","unstructured":"Whitesides GM (2006) The origins and the future of microfluidics. Nature 442:368\u2013373. https:\/\/doi.org\/10.1038\/nature05058","journal-title":"Nature"},{"key":"7_CR21","doi-asserted-by":"publisher","first-page":"647","DOI":"10.1016\/j.snb.2017.08.084","volume":"255","author":"Y Zheng","year":"2018","unstructured":"Zheng Y, Zhao G, Zhang YT, Gao R (2018) On-chip loading and unloading of cryoprotectants facilitate cell cryopreservation by rapid freezing. Sens Actuators B Chem 255:647\u2013656. https:\/\/doi.org\/10.1016\/j.snb.2017.08.084","journal-title":"Sens Actuators B Chem"},{"key":"7_CR22","doi-asserted-by":"publisher","first-page":"1874","DOI":"10.1039\/b823062e","volume":"9","author":"YS Song","year":"2009","unstructured":"Song YS, Moon S, Hulli L, Hasan SK, Kayaalp E, Demirci U (2009) Microfluidics for cryopreservation. Lab Chip 9:1874\u20131881. https:\/\/doi.org\/10.1039\/b823062e","journal-title":"Lab Chip"},{"key":"7_CR23","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1115\/1.1784474","volume":"126","author":"H Takamatsu","year":"2004","unstructured":"Takamatsu H, Komori Y, Zawlodzka S, Fujii M (2004) Quantitative examination of a perfusion microscope for the study of osmotic response of cells. J Biomech Eng 126:402\u2013409. https:\/\/doi.org\/10.1115\/1.1784474","journal-title":"J Biomech Eng"},{"key":"7_CR24","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1093\/humrep\/deu284","volume":"30","author":"D Lai","year":"2015","unstructured":"Lai D, Ding J, Smith GW, Smith GD, Takayama S (2015) Slow and steady cell shrinkage reduces osmotic stress in bovine and murine oocyte and zygote vitrification. Hum Reprod 30:37\u201345. https:\/\/doi.org\/10.1093\/humrep\/deu284","journal-title":"Hum Reprod"},{"key":"7_CR25","doi-asserted-by":"publisher","first-page":"3530","DOI":"10.1039\/c1lc20377k","volume":"11","author":"YS Heo","year":"2011","unstructured":"Heo YS, Lee HJ, Hassell BA, Irimia D, Toth TL, Elmoazzen H, Toner M (2011) Controlled loading of cryoprotectants (CPAs) to oocyte with linear and complex CPA profiles on a microfluidic platform. Lab Chip 11:3530\u20133537. https:\/\/doi.org\/10.1039\/c1lc20377k","journal-title":"Lab Chip"},{"key":"7_CR26","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1016\/j.snb.2017.12.034","volume":"259","author":"CY Lee","year":"2018","unstructured":"Lee CY, Fu LM (2018) Recent advances and applications of micromixers. Sens Actuators B Chem 259:677\u2013702. https:\/\/doi.org\/10.1016\/j.snb.2017.12.034","journal-title":"Sens Actuators B Chem"},{"key":"7_CR27","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1142\/9789814733199_0007","volume-title":"Multiscale Technologies for Cryomedicine: Implementation from Nano to Macroscale","author":"G Zhao","year":"2016","unstructured":"Zhao G, Huang Z, Gao D (2016) Microdevices for measurement of cell membrane biophysical properties. In: He X, Bischof JC (Eds.) Multiscale Technologies for Cryomedicine: Implementation from Nano to Macroscale. World Scientific Publishing Co. Pte. Ltd., Singapore, 221\u2013273"},{"key":"7_CR28","doi-asserted-by":"publisher","first-page":"031301","DOI":"10.1063\/5.0251893","volume":"19","author":"J Ko","year":"2025","unstructured":"Ko J, Lee J (2025) Advanced microfluidic systems with temperature modulation for biological applications. Biomicrofluidics 19:031301. https:\/\/doi.org\/10.1063\/5.0251893","journal-title":"Biomicrofluidics"},{"key":"7_CR29","unstructured":"Yager P (2025) Microfluidics tutorial: basic concepts. University of Washington. https:\/\/faculty.washington.edu\/yagerp\/microfluidicstutorial\/basicconcepts\/basicconcepts.htm"},{"key":"7_CR30","doi-asserted-by":"crossref","unstructured":"Fan J, Li S, Wu Z, Chen Z (2019) Diffusion and mixing in microfluidic devices. In: Santos HA, Liu D, Zhang H (eds) Microfluidics for pharmaceutical applications. William Andrew Publishing, pp 79\u2013100","DOI":"10.1016\/B978-0-12-812659-2.00003-X"},{"key":"7_CR31","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/B978-008044527-4\/50005-0","volume-title":"In: Heat Transfer and Fluid Flow in Minichannels and Microchannels","author":"SG Kandlikar","year":"2006","unstructured":"Kandlikar, SG, 2006. Single-phase liquid flow in minichannels and microchannels, In: Heat Transfer and Fluid Flow in Minichannels and Microchannels. Elsevier, pp. 87\u2013136"},{"key":"7_CR32","doi-asserted-by":"crossref","unstructured":"Bruus H (1997) Theoretical microfluidics. Oxford University Press","DOI":"10.1093\/oso\/9780199235087.001.0001"},{"key":"7_CR33","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1016\/j.ijthermalsci.2004.01.003","volume":"43","author":"GL Morini","year":"2004","unstructured":"Morini GL (2004) Single-phase convective heat transfer in microchannels: a review of experimental results. Int J Therm Sci 43:631\u2013651. https:\/\/doi.org\/10.1016\/j.ijthermalsci.2004.01.003","journal-title":"Int J Therm Sci"},{"key":"7_CR34","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1016\/j.ijheatmasstransfer.2013.03.045","volume":"62","author":"L Chai","year":"2013","unstructured":"Chai L, Xia G, Wang L, Zhou M, Cui Z (2013) Heat transfer enhancement in microchannel heat sinks with periodic expansion-constriction cross-sections. Int J Heat Mass Transfer 62:741\u2013751. https:\/\/doi.org\/10.1016\/j.ijheatmasstransfer.2013.03.045","journal-title":"Int J Heat Mass Transfer"},{"key":"7_CR35","doi-asserted-by":"publisher","first-page":"3993","DOI":"10.1016\/j.ijheatmasstransfer.2004.04.016","volume":"47","author":"G Maranzana","year":"2004","unstructured":"Maranzana G, Perry I, Maillet D (2004) Mini- and micro-channels: influence of axial conduction in the walls. Int J Heat Mass Transfer 47:3993\u20134004. https:\/\/doi.org\/10.1016\/j.ijheatmasstransfer.2004.04.016","journal-title":"Int J Heat Mass Transfer"},{"key":"7_CR36","doi-asserted-by":"publisher","first-page":"977","DOI":"10.1103\/revmodphys.77.977","volume":"77","author":"TM Squires","year":"2005","unstructured":"Squires TM, Quake SR (2005) Microfluidics: fluid physics at the nanoliter scale. Rev Mod Phys 77:977\u20131026. https:\/\/doi.org\/10.1103\/revmodphys.77.977","journal-title":"Rev Mod Phys"},{"key":"7_CR37","volume-title":"Transport phenomena","author":"RB Bird","year":"2002","unstructured":"Bird, RB, Stewart, WE, Lightfoot, EN, 2002. Transport phenomena, 2nd edn. Wiley, New York","edition":"2nd edn"},{"key":"7_CR38","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1146\/annurev.bioeng.4.112601.125916","volume":"4","author":"DJ Beebe","year":"2002","unstructured":"Beebe DJ, Mensing GA, Walker GM (2002) Physics and applications of microfluidics in biology. Annu Rev Biomed Eng 4:261\u2013286. https:\/\/doi.org\/10.1146\/annurev.bioeng.4.112601.125916","journal-title":"Annu Rev Biomed Eng"},{"key":"7_CR39","doi-asserted-by":"publisher","first-page":"2316","DOI":"10.1002\/bit.24499","volume":"109","author":"J Hanna","year":"2012","unstructured":"Hanna J, Hubel A, Lemke E (2012) Diffusion-based extraction of DMSO from a cell suspension in a three stream, vertical microchannel. Biotechnol Bioeng 109:2316\u20132324. https:\/\/doi.org\/10.1002\/bit.24499","journal-title":"Biotechnol Bioeng"},{"key":"7_CR40","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1006\/cryo.1997.2013","volume":"34","author":"JJ McGrath","year":"1997","unstructured":"McGrath JJ (1997) Quantitative measurement of cell membrane transport: technology and applications. Cryobiology 34:315\u2013334. https:\/\/doi.org\/10.1006\/cryo.1997.2013","journal-title":"Cryobiology"},{"key":"7_CR41","doi-asserted-by":"publisher","first-page":"1297","DOI":"10.1039\/c6lc01532h","volume":"17","author":"G Zhao","year":"2017","unstructured":"Zhao G, Zhang Z, Zhang Y, Chen Z, Niu D, Cao Y, He X (2017) A microfluidic perfusion approach for on-chip characterization of the transport properties of human oocytes. Lab Chip 17:1297\u20131305. https:\/\/doi.org\/10.1039\/c6lc01532h","journal-title":"Lab Chip"},{"key":"7_CR42","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1016\/s0006-3495(96)79246-9","volume":"71","author":"DY Gao","year":"1996","unstructured":"Gao DY, Benson CT, Liu C, McGrath JJ, Critser ES, Critser JK (1996) Development of a novel microperfusion chamber for determination of cell membrane transport properties. Biophys J 71:443\u2013450. https:\/\/doi.org\/10.1016\/s0006-3495(96)79246-9","journal-title":"Biophys J"},{"issue":"3","key":"7_CR43","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1111\/j.1365-2818.1985.tb02641.x","volume":"139","author":"JJ McGrath","year":"1985","unstructured":"McGrath JJ (1985) A microscope diffusion chamber for the determination of the equilibrium and non-equilibrium osmotic response of individual cells. J Microsc 139(3):249\u2013263. https:\/\/doi.org\/10.1111\/j.1365-2818.1985.tb02641.x","journal-title":"J Microsc"},{"key":"7_CR44","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1089\/bio.2011.0027","volume":"9","author":"HY Tseng","year":"2011","unstructured":"Tseng HY, Sun S, Shu Z, Ding W, Reems JA, Gao D (2011) A microfluidic study of megakaryocytes membrane transport properties to water and dimethyl sulfoxide at suprazero and subzero temperatures. Biopreserv Biobank 9:355\u2013362. https:\/\/doi.org\/10.1089\/bio.2011.0027","journal-title":"Biopreserv Biobank"},{"key":"7_CR45","doi-asserted-by":"publisher","first-page":"5687","DOI":"10.1016\/j.ijheatmasstransfer.2008.04.013","volume":"51","author":"HH Chen","year":"2008","unstructured":"Chen HH, Lin EH, Heimfeld S, Gao D (2008) A microfluidic study of mouse dendritic cell membrane transport properties of water and cryoprotectants. Int J Heat Mass Transfer 51:5687\u20135694. https:\/\/doi.org\/10.1016\/j.ijheatmasstransfer.2008.04.013","journal-title":"Int J Heat Mass Transfer"},{"key":"7_CR46","doi-asserted-by":"publisher","first-page":"951","DOI":"10.1039\/c6lc01523a","volume":"17","author":"CF Fang","year":"2017","unstructured":"Fang CF, Ji F, Shu Z, Gao D (2017) Determination of the temperature-dependent cell membrane permeabilities using microfluidics with integrated flow and temperature control. Lab Chip 17:951\u2013960. https:\/\/doi.org\/10.1039\/c6lc01523a","journal-title":"Lab Chip"},{"key":"7_CR47","doi-asserted-by":"publisher","first-page":"1929","DOI":"10.1039\/c9lc00107g","volume":"19","author":"ZL Lei","year":"2019","unstructured":"Lei ZL, Xie D, Mbogba MK, Chen Z, Tian C, Xu L, Zhao G (2019) A microfluidic platform with cell-scale precise temperature control for simultaneous investigation of the osmotic responses of multiple oocytes. Lab Chip 19:1929\u20131940. https:\/\/doi.org\/10.1039\/c9lc00107g","journal-title":"Lab Chip"},{"key":"7_CR48","first-page":"232","volume":"33","author":"G Zhao","year":"2012","unstructured":"Zhao G, Kurata K, Takamatsu H (2012) Measurement of membrane hydraulic conductivity of bovine carotid artery endothelial cells using a perfusion microscope. CryoLetters 33:232\u2013240","journal-title":"CryoLetters"},{"key":"7_CR49","doi-asserted-by":"publisher","first-page":"869","DOI":"10.1016\/j.ijheatmasstransfer.2015.03.038","volume":"86","author":"W Liu","year":"2015","unstructured":"Liu W, Zhao G, Shu ZQ, Wang T, Zhu K, Gao D (2015) High-precision approach based on microfluidic perfusion chamber for quantitative analysis of biophysical properties of cell membrane. Int J Heat Mass Transfer 86:869\u2013879. https:\/\/doi.org\/10.1016\/j.ijheatmasstransfer.2015.03.038","journal-title":"Int J Heat Mass Transfer"},{"key":"7_CR50","doi-asserted-by":"publisher","first-page":"338441","DOI":"10.1016\/j.aca.2021.338441","volume":"1163","author":"L Huang","year":"2021","unstructured":"Huang L, Benson JD, Almasri M (2021) Microfluidic measurement of individual cell membrane water permeability. Anal Chim Acta 1163:338441. https:\/\/doi.org\/10.1016\/j.aca.2021.338441","journal-title":"Anal Chim Acta"},{"key":"7_CR51","doi-asserted-by":"publisher","first-page":"341226","DOI":"10.1016\/j.aca.2023.341226","volume":"1267","author":"L Huang","year":"2023","unstructured":"Huang L, Jasim I, Alkorjia O, Agca C, Oksman A, Agca Y, Goldberg DE, Benson JD, Almasri M (2023) An impedance based microfluidic sensor for evaluation of individual red blood cell solute permeability. Anal Chim Acta 1267:341226. https:\/\/doi.org\/10.1016\/j.aca.2023.341226","journal-title":"Anal Chim Acta"},{"key":"7_CR52","doi-asserted-by":"publisher","first-page":"085035","DOI":"10.1088\/0960-1317\/20\/8\/085035","volume":"20","author":"Y Wu","year":"2010","unstructured":"Wu Y, Benson JD, Critser JK, Almasri M (2010) MEMS-based Coulter counter for cell counting and sizing using multiple electrodes. J Micromech Microeng 20:085035. https:\/\/doi.org\/10.1088\/0960-1317\/20\/8\/085035","journal-title":"J Micromech Microeng"},{"key":"7_CR53","doi-asserted-by":"publisher","first-page":"739","DOI":"10.1007\/s10544-012-9655-6","volume":"14","author":"Y Wu","year":"2012","unstructured":"Wu Y, Benson JD, Almasri M (2012) Micromachined Coulter counter for dynamic impedance study of time sensitive cells. Biomed Microdevices 14:739\u2013750. https:\/\/doi.org\/10.1007\/s10544-012-9655-6","journal-title":"Biomed Microdevices"},{"key":"7_CR54","doi-asserted-by":"publisher","unstructured":"Gao D, Zhou X (2012) Prevention of lethal osmotic injury to cells during addition and removal of cryoprotective agents: theory and technology. In: Katkov II (ed) Current frontiers in cryobiology. IntechOpen, Rijeka. https:\/\/doi.org\/10.5772\/34473","DOI":"10.5772\/34473"},{"key":"7_CR55","doi-asserted-by":"publisher","first-page":"5749","DOI":"10.1016\/j.ijheatmasstransfer.2008.04.018","volume":"51","author":"KK Fleming Glass","year":"2008","unstructured":"Fleming Glass KK, Longmire EK, Hubel A (2008) Optimization of a microfluidic device for diffusion-based extraction of DMSO from a cell suspension. Int J Heat Mass Transfer 51:5749\u20135757. https:\/\/doi.org\/10.1016\/j.ijheatmasstransfer.2008.04.018","journal-title":"Int J Heat Mass Transfer"},{"key":"7_CR56","doi-asserted-by":"publisher","first-page":"719","DOI":"10.3969\/j.issn.0258-8021.2019.06.010","volume":"38","author":"X Zhou","year":"2019","unstructured":"Zhou X, Du Y, Yi X, Dai J, Zhang D (2019) Optimization of cryoprotectants addition and removal protocols using microfluidic chip. Chinese J Biomed Eng 38:719\u2013725. https:\/\/doi.org\/10.3969\/j.issn.0258-8021.2019.06.010","journal-title":"Chinese J Biomed Eng"},{"key":"7_CR57","first-page":"37","volume":"38","author":"Y Yang","year":"2017","unstructured":"Yang Y, Zhou XL, Zhou NF, Shao WQ, Tao LR (2017) Numerical and experimental investigation on water-Me2SO mixing in microfluidic device for oocyte cryopreservation. CryoLetters 38:37\u201342","journal-title":"CryoLetters"},{"key":"7_CR58","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1016\/j.cryobiol.2012.01.001","volume":"64","author":"JD Benson","year":"2012","unstructured":"Benson JD, Kearsley AJ, Higgins AZ (2012) Mathematical optimization of procedures for cryoprotectant equilibration using a toxicity cost function. Cryobiology 64:144\u2013151. https:\/\/doi.org\/10.1016\/j.cryobiol.2012.01.001","journal-title":"Cryobiology"},{"key":"7_CR59","doi-asserted-by":"publisher","first-page":"e0142828","DOI":"10.1371\/journal.pone.0142828","volume":"10","author":"AF Davidson","year":"2015","unstructured":"Davidson AF, Glasscock C, McClanahan DR, Benson JD, Higgins AZ (2015) Toxicity minimized cryoprotectant addition and removal procedures for adherent endothelial cells. PLoS One 10:e0142828. https:\/\/doi.org\/10.1371\/journal.pone.0142828","journal-title":"PLoS One"},{"key":"7_CR60","doi-asserted-by":"publisher","first-page":"054124","DOI":"10.1063\/1.4900675","volume":"8","author":"RE Lusianti","year":"2014","unstructured":"Lusianti RE, Higgins AZ (2014) Continuous removal of glycerol from frozen-thawed red blood cells in a microfluidic membrane device. Biomicrofluidics 8:054124. https:\/\/doi.org\/10.1063\/1.4900675","journal-title":"Biomicrofluidics"},{"key":"7_CR61","doi-asserted-by":"publisher","first-page":"1428","DOI":"10.1039\/b705809h","volume":"7","author":"U Demirci","year":"2007","unstructured":"Demirci U, Montesano G (2007) Cell encapsulating droplet vitrification. Lab Chip 7:1428. https:\/\/doi.org\/10.1039\/b705809h","journal-title":"Lab Chip"},{"key":"7_CR62","doi-asserted-by":"publisher","first-page":"1700988","DOI":"10.1002\/adhm.201700988","volume":"6","author":"G Zhao","year":"2017","unstructured":"Zhao G, Liu X, Zhu K, He X (2017) Hydrogel encapsulation facilitates rapid-cooling cryopreservation of stem cell-laden core-shell microcapsules as cell-biomaterial constructs. Adv Healthcare Mater 6:1700988. https:\/\/doi.org\/10.1002\/adhm.201700988","journal-title":"Adv Healthcare Mater"},{"key":"7_CR63","first-page":"151","volume":"28","author":"A Sakai","year":"2007","unstructured":"Sakai A, Engelmann F (2007) Vitrification, encapsulation-vitrification and droplet-vitrification: a review. CryoLetters 28:151\u2013172","journal-title":"CryoLetters"},{"key":"7_CR64","doi-asserted-by":"publisher","first-page":"1151","DOI":"10.1021\/acsbiomaterials.2c01087","volume":"9","author":"M Cui","year":"2023","unstructured":"Cui M, Zhan T, Yang J, Dang H, Yang G, Han H, Liu L, Xu Y (2023) Droplet generation, vitrification, and warming for cell cryopreservation: a review. ACS Biomater Sci Eng 9:1151\u20131163. https:\/\/doi.org\/10.1021\/acsbiomaterials.2c01087","journal-title":"ACS Biomater Sci Eng"},{"key":"7_CR65","doi-asserted-by":"publisher","first-page":"7738","DOI":"10.1073\/pnas.1808645116","volume":"116","author":"Y Akiyama","year":"2019","unstructured":"Akiyama Y, Shinose M, Watanabe H, Yamada S, Kanda Y (2019) Cryoprotectant-free cryopreservation of mammalian cells by superflash freezing. Proc Natl Acad Sci USA 116:7738\u20137743. https:\/\/doi.org\/10.1073\/pnas.1808645116","journal-title":"Proc Natl Acad Sci USA"},{"key":"7_CR66","doi-asserted-by":"publisher","first-page":"7964","DOI":"10.1021\/acs.chemrev.6b00848","volume":"117","author":"L Shang","year":"2017","unstructured":"Shang L, Cheng Y, Zhao Y (2017) Emerging droplet microfluidics. Chem Rev 117:7964\u20138040. https:\/\/doi.org\/10.1021\/acs.chemrev.6b00848","journal-title":"Chem Rev"},{"key":"7_CR67","doi-asserted-by":"publisher","first-page":"16822","DOI":"10.1021\/acsami.8b04496","volume":"10","author":"X Liu","year":"2018","unstructured":"Liu X, Zhao G, Chen Z, Panhwar F, He X (2018) Dual suppression effect of magnetic induction heating and microencapsulation on ice crystallization enables low-cryoprotectant vitrification of stem cell-alginate hydrogel constructs. ACS Appl Mater Interfaces 10:16822\u201316835. https:\/\/doi.org\/10.1021\/acsami.8b04496","journal-title":"ACS Appl Mater Interfaces"},{"key":"7_CR68","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1007\/s40820-023-01213-3","volume":"16","author":"X Qin","year":"2023","unstructured":"Qin X, Chen Z, Shen L, Liu H, Ouyang X, Zhao G (2023) Core-shell microfiber encapsulation enables glycerol-free cryopreservation of RBCs with high hematocrit. Nanomicro Lett 16:3. https:\/\/doi.org\/10.1007\/s40820-023-01213-3","journal-title":"Nanomicro Lett"},{"key":"7_CR69","doi-asserted-by":"publisher","first-page":"e61593","DOI":"10.1371\/journal.pone.0061593","volume":"8","author":"YJ Zou","year":"2013","unstructured":"Zou YJ, Yin T, Chen S, Yang J, Huang W (2013) On-chip cryopreservation: a novel method for ultra-rapid cryoprotectant-free cryopreservation of small amounts of human spermatozoa. PLoS One 8:e61593. https:\/\/doi.org\/10.1371\/journal.pone.0061593","journal-title":"PLoS One"},{"key":"7_CR70","doi-asserted-by":"publisher","first-page":"484","DOI":"10.1126\/science.282.5388.484","volume":"282","author":"MA Burns","year":"1998","unstructured":"Burns MA, Johnson BN, Brahmasandra SN, Handique K, Webster JR, Krishnan M, Sammarco TS, Man PM, Jones D, Heldsinger D, Mastrangelo CH, Burke DT (1998) An integrated nanoliter DNA analysis device. Science 282:484\u2013487. https:\/\/doi.org\/10.1126\/science.282.5388.484","journal-title":"Science"},{"key":"7_CR71","doi-asserted-by":"publisher","first-page":"1046","DOI":"10.1126\/science.280.5366.1046","volume":"280","author":"MU Kopp","year":"1998","unstructured":"Kopp MU, Mello AJd, Manz A (1998) Chemical amplification: continuous-flow PCR on a chip. Science 280:1046\u20131048. https:\/\/doi.org\/10.1126\/science.280.5366.1046","journal-title":"Science"},{"key":"7_CR72","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1007\/s00216-014-8297-3","volume":"407","author":"C Hoera","year":"2014","unstructured":"Hoera C, Ohla S, Shu Z, Beckert E, Nagl S, Belder D (2014) An integrated microfluidic chip enabling control and spatially resolved monitoring of temperature in micro flow reactors. Anal Bioanal Chem 407:387\u2013396. https:\/\/doi.org\/10.1007\/s00216-014-8297-3","journal-title":"Anal Bioanal Chem"},{"key":"7_CR73","doi-asserted-by":"publisher","first-page":"33","DOI":"10.3390\/diagnostics3010033","volume":"3","author":"V Miralles","year":"2013","unstructured":"Miralles V, Huerre A, Malloggi F, Jullien MC (2013) A review of heating and temperature control in microfluidic systems: techniques and applications. Diagnostics 3:33\u201367. https:\/\/doi.org\/10.3390\/diagnostics3010033","journal-title":"Diagnostics"},{"key":"7_CR74","doi-asserted-by":"publisher","first-page":"660","DOI":"10.1016\/j.ijheatmasstransfer.2018.10.135","volume":"130","author":"J Peng","year":"2019","unstructured":"Peng J, Fang C, Ren S, Pan J, Jia Y, Shu Z, Gao D (2019) Development of a microfluidic device with precise on-chip temperature control by integrated cooling and heating components for single cell-based analysis. Int J Heat Mass Transfer 130:660\u2013667. https:\/\/doi.org\/10.1016\/j.ijheatmasstransfer.2018.10.135","journal-title":"Int J Heat Mass Transfer"},{"key":"7_CR75","doi-asserted-by":"publisher","first-page":"85620","DOI":"10.1039\/c5ra19944a","volume":"5","author":"C Fang","year":"2015","unstructured":"Fang C, Lee D, Stober B, Fuller GG, Shen AQ (2015) Integrated microfluidic platform for instantaneous flow and localized temperature control. RSC Adv 5:85620\u201385629. https:\/\/doi.org\/10.1039\/c5ra19944a","journal-title":"RSC Adv"},{"key":"7_CR76","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1016\/j.ijheatmasstransfer.2013.06.022","volume":"65","author":"X Zhou","year":"2013","unstructured":"Zhou X, Liu Z, Liang XM, Shu Z, Du P, Gao D (2013) Theoretical investigations of a novel microfluidic cooling\/warming system for cell vitrification cryopreservation. Int J Heat Mass Transfer 65:381\u2013388. https:\/\/doi.org\/10.1016\/j.ijheatmasstransfer.2013.06.022","journal-title":"Int J Heat Mass Transfer"},{"key":"7_CR77","doi-asserted-by":"publisher","first-page":"2293","DOI":"10.1039\/b906198c","volume":"9","author":"CA Stan","year":"2009","unstructured":"Stan CA, Schneider GF, Shevkoplyas SS, Hashimoto M, Ibanescu M, Wiley BJ, Whitesides GM (2009) A microfluidic apparatus for the study of ice nucleation in supercooled water drops. Lab Chip 9:2293. https:\/\/doi.org\/10.1039\/b906198c","journal-title":"Lab Chip"},{"key":"7_CR78","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1038\/s41378-020-0160-4","volume":"6","author":"ZR Chen","year":"2020","unstructured":"Chen ZR, Memon K, Cao Y, Zhao G (2020) A microfluidic approach for synchronous and nondestructive study of the permeability of multiple oocytes. Microsyst Nanoeng 6:55. https:\/\/doi.org\/10.1038\/s41378-020-0160-4","journal-title":"Microsyst Nanoeng"},{"key":"7_CR79","doi-asserted-by":"publisher","first-page":"6304","DOI":"10.1021\/acs.langmuir.3c03798","volume":"40","author":"L-T Deck","year":"2024","unstructured":"Deck L-T, Shardt N, El-Bakouri I, Isenrich FN, Marcolli C, deMello AJ, Mazzotti M (2024) Monitoring aqueous sucrose solutions using droplet microfluidics: ice nucleation, growth, glass transition, and melting. Langmuir 40:6304\u20136316. https:\/\/doi.org\/10.1021\/acs.langmuir.3c03798","journal-title":"Langmuir"},{"key":"7_CR80","first-page":"279","volume":"31","author":"G Zhao","year":"2010","unstructured":"Zhao G (2010) A universal self-adaptive time-varying function for extracellular concentration during osmotic shift for curve-fitting permeability coefficients of cell membrane. CryoLetters 31:279\u2013290","journal-title":"CryoLetters"},{"key":"7_CR81","doi-asserted-by":"publisher","first-page":"e72836","DOI":"10.1371\/journal.pone.0072836","volume":"8","author":"JY Wang","year":"2013","unstructured":"Wang JY, Zhu K, Zhao G, Ren J, Yue C, Gao D (2013) Dual dependence of cryobiogical properties of Sf21 cell membrane on the temperature and the concentration of the cryoprotectant. PLoS One 8:e72836. https:\/\/doi.org\/10.1371\/journal.pone.0072836","journal-title":"PLoS One"},{"key":"7_CR82","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1016\/j.cryobiol.2014.10.002","volume":"69","author":"JY Wang","year":"2014","unstructured":"Wang JY, Zhao G, Zhang P, Wang Z, Zhang Y, Gao D, Zhou P, Cao Y (2014) Measurement of the biophysical properties of porcine adipose-derived stem cells by a microperfusion system. Cryobiology 69:442\u2013450. https:\/\/doi.org\/10.1016\/j.cryobiol.2014.10.002","journal-title":"Cryobiology"},{"key":"7_CR83","doi-asserted-by":"publisher","first-page":"270","DOI":"10.1089\/ten.tec.2015.0364","volume":"22","author":"D Niu","year":"2016","unstructured":"Niu D, Zhao G, Liu X, Zhou P, Cao Y (2016) Prevention of osmotic injury to human umbilical vein endothelial cells for biopreservation: a first step toward biobanking of endothelial cells for vascular tissue engineering. Tissue Eng Part C Methods 22:270\u2013279. https:\/\/doi.org\/10.1089\/ten.tec.2015.0364","journal-title":"Tissue Eng Part C Methods"},{"key":"7_CR84","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.cryobiol.2015.12.002","volume":"72","author":"JY Wang","year":"2016","unstructured":"Wang JY, Zhao G, Shu Z, Zhou P, Cao Y, Gao D (2016) Effect of iron oxide nanoparticles on the permeability properties of Sf21 cells. Cryobiology 72:21\u201326. https:\/\/doi.org\/10.1016\/j.cryobiol.2015.12.002","journal-title":"Cryobiology"},{"key":"7_CR85","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/j.cryobiol.2017.09.006","volume":"79","author":"L Li","year":"2017","unstructured":"Li L, Chen Z, Zhang M, Panhwar F, Gao C, Zhao G, Jin B, Ye B (2017) Cell membrane permeability coefficients determined by single-step osmotic shift are not applicable for optimization of multi-step addition of cryoprotective agents: as revealed by HepG2 cells. Cryobiology 79:82\u201386. https:\/\/doi.org\/10.1016\/j.cryobiol.2017.09.006","journal-title":"Cryobiology"},{"key":"7_CR86","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1021\/acssensors.8b01215","volume":"4","author":"ZR Chen","year":"2019","unstructured":"Chen ZR, Zhang Z, Guo X, Memon K, Panhwar F, Wang M, Cao Y, Zhao G (2019) Sensing cell membrane biophysical properties for detection of high quality human oocytes. ACS Sens 4:192\u2013199. https:\/\/doi.org\/10.1021\/acssensors.8b01215","journal-title":"ACS Sens"},{"key":"7_CR87","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1016\/j.cryobiol.2007.08.001","volume":"55","author":"HH Chen","year":"2007","unstructured":"Chen HH, Purtteman JJ, Heimfeld S, Folch A, Gao D (2007) Development of a microfluidic device for determination of cell osmotic behavior and membrane transport properties. Cryobiology 55:200\u2013209. https:\/\/doi.org\/10.1016\/j.cryobiol.2007.08.001","journal-title":"Cryobiology"},{"key":"7_CR88","doi-asserted-by":"publisher","first-page":"100715","DOI":"10.1016\/j.device.2025.100715","volume":"3","author":"L Zhan","year":"2025","unstructured":"Zhan L, Hinnen H, Gopinathan KA, Toner M (2025) Autonomous cryoprotectant loading of the oocyte using microfluidic transistors. Device 3:100715. https:\/\/doi.org\/10.1016\/j.device.2025.100715","journal-title":"Device"},{"key":"7_CR89","doi-asserted-by":"publisher","DOI":"10.1007\/s10544-017-0155-6","volume":"19","author":"YF Kang","year":"2017","unstructured":"Kang YF, Zou LL, Qiu BS, Liang X, Sun S, Gao D, Ding W (2017) Unloading of cryoprotectants from cryoprotectant-loaded cells on a microfluidic platform. Biomed Microdevices 19: 15. https:\/\/doi.org\/10.1007\/s10544-017-0155-6","journal-title":"Biomed Microdevices"},{"key":"7_CR90","doi-asserted-by":"publisher","first-page":"1805361","DOI":"10.1002\/smll.201805361","volume":"15","author":"U Farooq","year":"2019","unstructured":"Farooq U, Haider Z, Liang XM, Memon K, Hossain SMC, Zheng Y, Xu H, Qadir A, Panhwar F, Dong S, Zhao G (2019) Surface-acoustic-wave-based lab-on-chip for rapid transport of cryoprotectants across cell membrane for cryopreservation with significantly improved cell viability. Small 15:1805361. https:\/\/doi.org\/10.1002\/smll.201805361","journal-title":"Small"},{"key":"7_CR91","doi-asserted-by":"publisher","first-page":"34443","DOI":"10.1039\/c4ra06972b","volume":"4","author":"L Li","year":"2014","unstructured":"Li L, Lv XQ, Guo H, Shi X, Liu J (2014) On-chip direct freezing and thawing of mammalian cells. RSC Adv 4:34443\u201334447. https:\/\/doi.org\/10.1039\/c4ra06972b","journal-title":"RSC Adv"},{"key":"7_CR92","doi-asserted-by":"publisher","first-page":"2400981","DOI":"10.1002\/adhm.202400981","volume":"13","author":"YF Li","year":"2024","unstructured":"Li YF, Zhang JX, Han W, Liu B, Zhai M, Li N, Wang Z, Zhao J (2024) Multifunctional laser-induced graphene-based microfluidic chip for high-performance oocyte cryopreservation with low concentration of cryoprotectants. Adv Healthc Mater 13:2400981. https:\/\/doi.org\/10.1002\/adhm.202400981","journal-title":"Adv Healthc Mater"},{"key":"7_CR93","doi-asserted-by":"publisher","first-page":"763","DOI":"10.16476\/j.pibb.2018.0016","volume":"45","author":"XL Zhou","year":"2018","unstructured":"Zhou XL, Guo YY, Yi XY et al. (2018) Experimental study of microfluidic chip for cryopreservation of oocytes. Prog Biochem Biophys 45:763\u2013771. https:\/\/doi.org\/10.16476\/j.pibb.2018.0016","journal-title":"Prog Biochem Biophys"},{"key":"7_CR94","doi-asserted-by":"publisher","first-page":"2503296","DOI":"10.1002\/adfm.202503296","volume":"35","author":"SY Xu","year":"2025","unstructured":"Xu SY, Lei Y, Wei ML, Fang Z, Cheng Y, Zhao G, Chen Z (2025) Transparent alumina ceramics-based microfluidic chip enables on-chip cryopreservation for mouse oocyte. Adv Funct Mater 35:2503296. https:\/\/doi.org\/10.1002\/adfm.202503296","journal-title":"Adv Funct Mater"},{"key":"7_CR95","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2121-11-54","volume":"11","author":"M Deutsch","year":"2010","unstructured":"Deutsch M, Afrimzon E, Namer Y, Shafran Y, Sobolev M, Zurgil N, Deutsch A, Howitz S, Greuner M, Thaele M, Zimmermann H, Meiser I, Ehrhart F (2010) The individual-cell-based cryo-chip for the cryopreservation, manipulation and observation of spatially identifiable cells. I: Methodology. BMC Cell Biol 11: 54. https:\/\/doi.org\/10.1186\/1471-2121-11-54","journal-title":"BMC Cell Biol"},{"key":"7_CR96","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2121-11-83","volume":"11","author":"E Afrimzon","year":"2010","unstructured":"Afrimzon E, Zurgil N, Shafran Y, Ehrhart F, Namer Y, Moshkov S, Sobolev M, Deutsch A, Howitz S, Greuner M, Thaele M, Meiser I, Zimmermann H, Deutsch M (2010) The individual-cell-based cryo-chip for the cryopreservation, manipulation and observation of spatially identifiable cells. II: Functional activity of cryopreserved cells. BMC Cell Biol 11: 83. https:\/\/doi.org\/10.1186\/1471-2121-11-83","journal-title":"BMC Cell Biol"},{"key":"7_CR97","doi-asserted-by":"publisher","first-page":"1478","DOI":"10.1093\/bbb\/zbad123","volume":"87","author":"XQ Lv","year":"2023","unstructured":"Lv XQ, Ma ZT, Guo L (2023) Freezing and thawing of cells on a microfluidic device: a simple and time-saving experimental procedure. Biosci Biotechnol Biochem 87:1478\u20131484. https:\/\/doi.org\/10.1093\/bbb\/zbad123","journal-title":"Biosci Biotechnol Biochem"},{"key":"7_CR98","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1002\/bit.25704","volume":"113","author":"E Kondo","year":"2016","unstructured":"Kondo E, Wada K, Hosokawa K, Maeda M (2016) Cryopreservation of adhered mammalian cells on a microfluidic device: Toward ready-to-use cell-based experimental platforms. Biotechnol Bioeng 113:237\u2013240. https:\/\/doi.org\/10.1002\/bit.25704","journal-title":"Biotechnol Bioeng"},{"key":"7_CR99","doi-asserted-by":"publisher","first-page":"622","DOI":"10.1021\/acsmacrolett.5c00129","volume":"14","author":"D Kim","year":"2025","unstructured":"Kim D, Yi S, Park BU, Lee SJ, Lee J, Kim DS, Kim HK (2025) Ready-to-use nanofiber membrane-integrated organ-on-a-chip via cryopreservation of cell-loaded microfluidic Chip. ACS Macro Lett 14:622\u2013629. https:\/\/doi.org\/10.1021\/acsmacrolett.5c00129","journal-title":"ACS Macro Lett"}],"container-title":["Methods in Molecular Biology","Cryopreservation and Freeze-Drying Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5416-3_7","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T06:26:31Z","timestamp":1789280791000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5416-3_7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071654156","9781071654163"],"references-count":99,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5416-3_7","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"14 September 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}