{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T06:35:02Z","timestamp":1793082902514,"version":"4.5.0"},"publisher-location":"New York, NY","reference-count":42,"publisher":"Springer US","isbn-type":[{"value":"9781071654873","type":"print"},{"value":"9781071654880","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5488-0_33","type":"book-chapter","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:19:01Z","timestamp":1788304741000},"page":"431-448","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High-Throughput Hypocotyl Grafting and Vascular Reconnection Assessment in Arabidopsis Seedlings"],"prefix":"10.1007","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2708-5545","authenticated-orcid":false,"given":"Kai","family":"Bartusch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5860-0234","authenticated-orcid":false,"given":"Elisabeth","family":"Truernit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,2]]},"reference":[{"key":"33_CR1","doi-asserted-by":"publisher","first-page":"2433","DOI":"10.1093\/jxb\/erad061","volume":"74","author":"G Loupit","year":"2023","unstructured":"Loupit G, Brocard L, Ollat N, Cookson SJ (2023) Grafting in plants: recent discoveries and new applications. J Exp Bot 74:2433\u20132447. https:\/\/doi.org\/10.1093\/jxb\/erad061","journal-title":"J Exp Bot"},{"key":"33_CR2","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/j.molp.2023.12.006","volume":"17","author":"M Feng","year":"2024","unstructured":"Feng M, Augstein F, Kareem A, Melnyk CW (2024) Plant grafting: molecular mechanisms and applications. Mol Plant 17:75\u201391. https:\/\/doi.org\/10.1016\/j.molp.2023.12.006","journal-title":"Mol Plant"},{"key":"33_CR3","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1002\/9780470593776.ch9","volume-title":"Horticultural reviews","author":"K Mudge","year":"2009","unstructured":"Mudge K, Janick J, Scofield S, Goldschmidt EE (2009) A history of grafting. In: Janick J (Ed.) Horticultural reviews. 1st edn. Wiley, New Jersey, 437\u2013493","edition":"1st edn"},{"key":"33_CR4","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/j.scienta.2010.08.003","volume":"127","author":"J-M Lee","year":"2010","unstructured":"Lee J-M, Kubota C, Tsao SJ et al. (2010) Current status of vegetable grafting: diffusion, grafting techniques, automation. Sci Hortic 127:93\u2013105.\u2002https:\/\/doi.org\/10.1016\/j.scienta.2010.08.003","journal-title":"Sci Hortic"},{"key":"33_CR5","doi-asserted-by":"publisher","first-page":"e70057","DOI":"10.1111\/tpj.70057","volume":"121","author":"F Augstein","year":"2025","unstructured":"Augstein F, Melnyk CW (2025) Modern and historical uses of plant grafting to engineer development, stress tolerance, chimeras, and hybrids. Plant J 121:e70057. https:\/\/doi.org\/10.1111\/tpj.70057","journal-title":"Plant J"},{"key":"33_CR6","doi-asserted-by":"publisher","first-page":"955","DOI":"10.1038\/s41477-025-01982-2","volume":"11","author":"F Kragler","year":"2025","unstructured":"Kragler F, Bock R (2025) The biology of grafting and its applications in studying information exchange between plants. Nat Plants 11:955\u2013966. https:\/\/doi.org\/10.1038\/s41477-025-01982-2","journal-title":"Nat Plants"},{"key":"33_CR7","doi-asserted-by":"publisher","first-page":"751","DOI":"10.1242\/dev.118.3.751","volume":"118","author":"H Tsukaya","year":"1993","unstructured":"Tsukaya H, Naito S, R\u00e9dei GP, Komeda Y (1993) A new class of mutations in Arabidopsis thaliana, acaulis1, affecting the development of both inflorescences and leaves. Development 118:751\u2013764. https:\/\/doi.org\/10.1242\/dev.118.3.751","journal-title":"Development"},{"key":"33_CR8","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1046\/j.1365-313X.2002.01419.x","volume":"32","author":"CGN Turnbull","year":"2002","unstructured":"Turnbull CGN, Booker JP, Leyser HMO (2002) Micrografting techniques for testing long-distance signalling in Arabidopsis. Plant J\u200232:255\u2013262.\u2002https:\/\/doi.org\/10.1046\/j.1365-313X.2002.01419.x","journal-title":"Plant J"},{"key":"33_CR9","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1385\/1-59745-003-0:39","volume":"323","author":"K Bainbridge","year":"2006","unstructured":"Bainbridge K, Bennett T, Turnbull C, Leyser O (2006) Grafting. Methods Mol Biol Clifton NJ 323:39\u201344. https:\/\/doi.org\/10.1385\/1-59745-003-0:39","journal-title":"Methods Mol Biol Clifton NJ"},{"key":"33_CR10","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1007\/s10265-008-0209-1","volume":"122","author":"M Notaguchi","year":"2009","unstructured":"Notaguchi M, Daimon Y, Abe M, Araki T (2009) Adaptation of a seedling micro-grafting technique to the study of long-distance signaling in flowering of Arabidopsis thaliana. J Plant Res 122:201\u2013214. https:\/\/doi.org\/10.1007\/s10265-008-0209-1","journal-title":"J Plant Res"},{"key":"33_CR11","doi-asserted-by":"publisher","DOI":"10.1186\/1746-4811-9-14","volume":"9","author":"N Marsch-Mart\u00ednez","year":"2013","unstructured":"Marsch-Mart\u00ednez N, Franken J, Gonzalez-Aguilera KL et al. (2013) An efficient flat-surface collar-free grafting method for Arabidopsis thaliana seedlings. Plant Methods 9: 14. https:\/\/doi.org\/10.1186\/1746-4811-9-14","journal-title":"Plant Methods"},{"key":"33_CR12","doi-asserted-by":"publisher","unstructured":"Andersen T, Liang D, Halkier B, White R (2014) Grafting Arabidopsis. BIO-Protoc 4:e1164. https:\/\/doi.org\/10.21769\/BioProtoc.1164","DOI":"10.21769\/BioProtoc.1164"},{"key":"33_CR13","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1007\/978-1-62703-580-4_7","volume-title":"Arabidopsis protocols","author":"K Bainbridge","year":"2014","unstructured":"Bainbridge K, Bennett T, Crisp P et al. (2014) Grafting in Arabidopsis. In: Sanchez-Serrano JJ, Salinas J (Eds.)Eds. Arabidopsis protocols. Humana Press, Totowa, NJ, 155\u2013163"},{"key":"33_CR14","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1007\/978-1-4939-6469-7_2","volume-title":"Plant hormones","author":"CW Melnyk","year":"2017","unstructured":"Melnyk CW (2017) Grafting with Arabidopsis thaliana. In: Kleine-Vehn J, Sauer M (Eds.)Eds. Plant hormones. Springer, New York, New York, NY, 9\u201318"},{"key":"33_CR15","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1007\/978-1-4939-6722-3_9","volume-title":"Xylem","author":"CW Melnyk","year":"2017","unstructured":"Melnyk CW (2017) Monitoring vascular regeneration and xylem connectivity in Arabidopsis thaliana. In: De Lucas M, Etchhells JP (Eds.)Eds. Xylem. Springer, New York, New York, NY, 91\u2013102"},{"key":"33_CR16","doi-asserted-by":"publisher","DOI":"10.1186\/s13007-019-0477-x","volume":"15","author":"Y Li","year":"2019","unstructured":"Li Y, Sun W, Liu F et al. (2019) Methods for grafting Arabidopsis thaliana and Eutrema salsugineum. Plant Methods 15: 93. https:\/\/doi.org\/10.1186\/s13007-019-0477-x","journal-title":"Plant Methods"},{"key":"33_CR17","doi-asserted-by":"publisher","first-page":"918","DOI":"10.1111\/tpj.14768","volume":"103","author":"H Tsutsui","year":"2020","unstructured":"Tsutsui H, Yanagisawa N, Kawakatsu Y et al. (2020) Micrografting device for testing systemic signaling in Arabidopsis. Plant J 103:918\u2013929. https:\/\/doi.org\/10.1111\/tpj.14768","journal-title":"Plant J"},{"key":"33_CR18","doi-asserted-by":"publisher","unstructured":"Tsutsui H, Kawakatsu Y, Notaguchi M (2021) Micrografting in Arabidopsis using a silicone chip. BIO-Protoc 11:e4053. https:\/\/doi.org\/10.21769\/BioProtoc.4053","DOI":"10.21769\/BioProtoc.4053"},{"key":"33_CR19","doi-asserted-by":"publisher","DOI":"10.1186\/s13007-022-00859-1","volume":"18","author":"L Vanderstraeten","year":"2022","unstructured":"Vanderstraeten L, Sanchez-Mu\u00f1oz R, Depaepe T et al. (2022) Mix-and-match: an improved, fast and accessible protocol for hypocotyl micrografting of Arabidopsis seedlings with systemic ACC responses as a case study. Plant Methods 18: 24. https:\/\/doi.org\/10.1186\/s13007-022-00859-1","journal-title":"Plant Methods"},{"key":"33_CR20","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1007\/978-1-0716-2156-1_8","volume-title":"Abscisic acid","author":"F Takahashi","year":"2022","unstructured":"Takahashi F (2022) Use of micrografting to study the role played by peptide signals in ABA biosynthesis. In: Yoshida T (Ed.)Eds. Abscisic acid. Springer, US, New York, NY, 101\u2013109"},{"key":"33_CR21","doi-asserted-by":"publisher","unstructured":"Yell V, Li X (2023) Scalable, flexible, and cost-effective seedling grafting. J Vis Exp 191:64519. https:\/\/doi.org\/10.3791\/64519","DOI":"10.3791\/64519"},{"key":"33_CR22","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1007\/978-1-0716-3183-6_10","volume-title":"Plant iron homeostasis","author":"E-J Hsieh","year":"2023","unstructured":"Hsieh E-J, Grillet L (2023) Arabidopsis micro-grafting to study the systemic signaling of nutrient status. In: Jeong J (Ed.)Eds. Plant iron homeostasis. Springer, US, New York, NY, 113\u2013120"},{"key":"33_CR23","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1667\/RR2486.1","volume":"176","author":"T Wang","year":"2011","unstructured":"Wang T, Li F, Xu S et al. (2011) The time course of long-distance signaling in radiation-induced bystander effect in vivo in Arabidopsis thaliana demonstrated using root micro-grafting. Radiat Res 176:234\u2013243. https:\/\/doi.org\/10.1667\/RR2486.1","journal-title":"Radiat Res"},{"key":"33_CR24","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1093\/pcp\/pcs158","volume":"54","author":"SJ Yoo","year":"2013","unstructured":"Yoo SJ, Hong SM, Jung HS, Ahn JH (2013) The cotyledons produce sufficient FT protein to induce flowering: evidence from cotyledon micrografting in Arabidopsis. Plant Cell Physiol 54:119\u2013128. https:\/\/doi.org\/10.1093\/pcp\/pcs158","journal-title":"Plant Cell Physiol"},{"key":"33_CR25","doi-asserted-by":"publisher","DOI":"10.1186\/s13007-020-0562-1","volume":"16","author":"K Bartusch","year":"2020","unstructured":"Bartusch K, Trenner J, Melnyk CW, Quint M (2020) Cut and paste: temperature-enhanced cotyledon micrografting for Arabidopsis thaliana seedlings. Plant Methods 16: 12. https:\/\/doi.org\/10.1186\/s13007-020-0562-1","journal-title":"Plant Methods"},{"key":"33_CR26","doi-asserted-by":"publisher","first-page":"1291","DOI":"10.1093\/pcp\/pcx098","volume":"58","author":"H Tsutsui","year":"2017","unstructured":"Tsutsui H, Notaguchi M (2017) The use of grafting to study systemic signaling in plants. Plant Cell Physiol 58:1291\u20131301. https:\/\/doi.org\/10.1093\/pcp\/pcx098","journal-title":"Plant Cell Physiol"},{"key":"33_CR27","doi-asserted-by":"publisher","first-page":"582","DOI":"10.1111\/nph.15772","volume":"223","author":"HR Thomas","year":"2019","unstructured":"Thomas HR, Frank MH (2019) Connecting the pieces: uncovering the molecular basis for long-distance communication through plant grafting. New Phytol 223:582\u2013589. https:\/\/doi.org\/10.1111\/nph.15772","journal-title":"New Phytol"},{"key":"33_CR28","doi-asserted-by":"publisher","first-page":"958","DOI":"10.1038\/s41587-022-01585-8","volume":"41","author":"L Yang","year":"2023","unstructured":"Yang L, Machin F, Wang S et al. (2023) Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks. Nat Biotechnol 41:958\u2013967.\u2002https:\/\/doi.org\/10.1038\/s41587-022-01585-8","journal-title":"Nat Biotechnol"},{"key":"33_CR29","doi-asserted-by":"publisher","first-page":"613442","DOI":"10.3389\/fpls.2020.613442","volume":"11","author":"K Bartusch","year":"2020","unstructured":"Bartusch K, Melnyk CW (2020) Insights into plant surgery: an overview of the multiple grafting techniques for Arabidopsis thaliana. Front Plant Sci 11:613442. https:\/\/doi.org\/10.3389\/fpls.2020.613442","journal-title":"Front Plant Sci"},{"key":"33_CR30","doi-asserted-by":"publisher","first-page":"dev200079","DOI":"10.1242\/dev.200079","volume":"149","author":"PT Serivichyaswat","year":"2022","unstructured":"Serivichyaswat PT, Bartusch K, Leso M et al. (2022) High temperature perception in leaves promotes vascular regeneration and graft formation in distant tissues. Development 149:dev200079.\u2002https:\/\/doi.org\/10.1242\/dev.200079","journal-title":"Development"},{"key":"33_CR31","doi-asserted-by":"publisher","first-page":"1306","DOI":"10.1016\/j.cub.2015.03.032","volume":"25","author":"CW Melnyk","year":"2015","unstructured":"Melnyk CW, Schuster C, Leyser O, Meyerowitz EM (2015) A developmental framework for graft formation and vascular reconnection in Arabidopsis thaliana. Curr Biol 25:1306\u20131318. https:\/\/doi.org\/10.1016\/j.cub.2015.03.032","journal-title":"Curr Biol"},{"key":"33_CR32","doi-asserted-by":"publisher","first-page":"1211","DOI":"10.1104\/pp.114.255414","volume":"167","author":"M Knoblauch","year":"2015","unstructured":"Knoblauch M, Vendrell M, De Leau E et al. (2015) Multispectral phloem-mobile probes: properties and applications. Plant Physiol 167:1211\u20131220.\u2002https:\/\/doi.org\/10.1104\/pp.114.255414","journal-title":"Plant Physiol"},{"key":"33_CR33","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1007\/978-1-0716-3477-6_1","volume-title":"Xylem","author":"K Bartusch","year":"2024","unstructured":"Bartusch K, Blanco-Touri\u00f1\u00e1n N, Rodriguez-Villal\u00f3n A, Truernit E (2024) Monitoring xylem transport in Arabidopsis thaliana seedlings using fluorescent dyes. In: Agusti J (Ed.)Eds. Xylem. Springer, US, New York, NY, 3\u201315"},{"key":"33_CR34","doi-asserted-by":"publisher","first-page":"15190","DOI":"10.1038\/nplants.2015.190","volume":"2","author":"M Quint","year":"2016","unstructured":"Quint M, Delker C, Franklin KA et al. (2016) Molecular and genetic control of plant thermomorphogenesis. Nat Plants 2:15190. https:\/\/doi.org\/10.1038\/nplants.2015.190","journal-title":"Nat Plants"},{"key":"33_CR35","doi-asserted-by":"publisher","first-page":"e210","DOI":"10.1371\/journal.pgen.0020210","volume":"2","author":"A Rus","year":"2006","unstructured":"Rus A, Baxter I, Muthukumar B et al. (2006) Natural variants of AtHKT1 enhance Na+ accumulation in two wild populations of Arabidopsis. PLoS Genet 2:e210. https:\/\/doi.org\/10.1371\/journal.pgen.0020210","journal-title":"PLoS Genet"},{"key":"33_CR36","doi-asserted-by":"publisher","first-page":"2745","DOI":"10.1016\/j.devcel.2024.06.019","volume":"59","author":"S Damodaran","year":"2024","unstructured":"Damodaran S, Strader LC (2024) Factors governing cellular reprogramming competence in Arabidopsis adventitious root formation. Dev Cell 59:2745\u20132758.e3. https:\/\/doi.org\/10.1016\/j.devcel.2024.06.019","journal-title":"Dev Cell"},{"key":"33_CR37","doi-asserted-by":"publisher","DOI":"10.1186\/s13007-016-0122-x","volume":"12","author":"A Ostendorp","year":"2016","unstructured":"Ostendorp A, Pahlow S, Deke J et al. (2016) Protocol: optimisation of a grafting protocol for oilseed rape (Brassica napus) for studying long-distance signalling. Plant Methods 12: 22. https:\/\/doi.org\/10.1186\/s13007-016-0122-x","journal-title":"Plant Methods"},{"key":"33_CR38","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-01158-w","volume":"11","author":"F Bantis","year":"2021","unstructured":"Bantis F, Panteris E, Dangitsis C et al. (2021) Blue light promotes vascular reconnection, while red light boosts the physiological response and quality of grafted watermelon seedlings. Sci Rep 11: 21754. https:\/\/doi.org\/10.1038\/s41598-021-01158-w","journal-title":"Sci Rep"},{"key":"33_CR39","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1038\/s41438-020-00390-1","volume":"7","author":"Y He","year":"2020","unstructured":"He Y, Zhang T, Sun H et al. (2020) A reporter for noninvasively monitoring gene expression and plant transformation. Hortic Res 7:152.\u2002https:\/\/doi.org\/10.1038\/s41438-020-00390-1","journal-title":"Hortic Res"},{"key":"33_CR40","doi-asserted-by":"publisher","first-page":"1614","DOI":"10.1128\/aem.61.4.1614-1619.1995","volume":"61","author":"P Breeuwer","year":"1995","unstructured":"Breeuwer P, Drocourt JL, Bunschoten N et al. (1995) Characterization of uptake and hydrolysis of fluorescein diacetate and carboxyfluorescein diacetate by intracellular esterases in Saccharomyces cerevisiae, which result in accumulation of fluorescent product. Appl Environ Microbiol 61:1614\u20131619. https:\/\/doi.org\/10.1128\/aem.61.4.1614-1619.1995","journal-title":"Appl Environ Microbiol"},{"key":"33_CR41","doi-asserted-by":"publisher","first-page":"4219","DOI":"10.1093\/jxb\/ers109","volume":"63","author":"H Yin","year":"2012","unstructured":"Yin H, Yan B, Sun J et al. (2012) Graft-union development: a delicate process that involves cell\u2013cell communication between scion and stock for local auxin accumulation. J Exp Bot 63:4219\u20134232. https:\/\/doi.org\/10.1093\/jxb\/ers109","journal-title":"J Exp Bot"},{"key":"33_CR42","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1105\/tpc.11.3.309","volume":"11","author":"A Imlau","year":"1999","unstructured":"Imlau A, Truernit E, Sauer N (1999) Cell-to-cell and long-distance trafficking of the green fluorescent protein in the phloem and symplastic unloading of the protein into sink tissues. Plant Cell 11:309\u2013322. https:\/\/doi.org\/10.1105\/tpc.11.3.309","journal-title":"Plant Cell"}],"updated-by":[{"DOI":"10.1007\/978-1-0716-5488-0_40","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T00:00:00Z","timestamp":1793145600000}}],"container-title":["Methods in Molecular Biology","Phloem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5488-0_33","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T05:57:01Z","timestamp":1793080621000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/chapter\/10.1007\/978-1-0716-5488-0_33"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071654873","9781071654880"],"references-count":42,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5488-0_33","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"2 September 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}},{"value":"28 October 2026","order":2,"name":"change_date","label":"Change Date","group":{"name":"ChapterHistory","label":"Chapter History"}},{"value":"Correction","order":3,"name":"change_type","label":"Change Type","group":{"name":"ChapterHistory","label":"Chapter History"}},{"value":"A correction has been published.","order":4,"name":"change_details","label":"Change Details","group":{"name":"ChapterHistory","label":"Chapter History"}},{"value":"The authors have no conflicts of interest to declare that are relevant to the content of this chapter.","order":1,"name":"Ethics","label":"<b>Conflict of Interest<\/b>","group":{"name":"EthicsHeading","label":"Ethics"}}]}}