{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T07:03:23Z","timestamp":1789369403300,"version":"build-2803163510"},"publisher-location":"New York, NY","reference-count":5,"publisher":"Springer US","isbn-type":[{"value":"9781071654958","type":"print"},{"value":"9781071654965","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2027]]},"DOI":"10.1007\/978-1-0716-5496-5_8","type":"book-chapter","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T06:08:59Z","timestamp":1789366139000},"page":"81-93","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Cloning and Reverse Genetics"],"prefix":"10.1007","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3255-0923","authenticated-orcid":false,"given":"Eugenia S.","family":"Bardossy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"8_CR1","doi-asserted-by":"publisher","first-page":"916","DOI":"10.1126\/science.6272391","volume":"214","author":"VR Racaniello","year":"1981","unstructured":"Racaniello VR, Baltimore D (1981) Cloned poliovirus complementary DNA is infectious in mammalian cells. Science 214:916\u2013919","journal-title":"Science"},{"key":"8_CR2","doi-asserted-by":"publisher","first-page":"6551","DOI":"10.1016\/S0021-9258(19)46967-6","volume":"253","author":"CA Hutchison","year":"1978","unstructured":"Hutchison CA, Phillips S, Edgell MH et al (1978) Mutagenesis at a specific position in a DNA sequence. J Biol Chem 253:6551\u20136560","journal-title":"J Biol Chem"},{"key":"8_CR3","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/0378-1119(89)90358-2","volume":"77","author":"SN Ho","year":"1989","unstructured":"Ho SN, Hunt HD, Horton RM et al (1989) Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77:51\u201359","journal-title":"Gene"},{"key":"8_CR4","doi-asserted-by":"publisher","first-page":"e0004402","DOI":"10.1371\/journal.pntd.0004402","volume":"10","author":"KA Stapleford","year":"2016","unstructured":"Stapleford KA, Moratorio G, Henningsson R et al (2016) Whole-genome sequencing analysis from the chikungunya virus Caribbean outbreak reveals novel evolutionary genomic elements. PLoS Negl Trop Dis 10:e0004402","journal-title":"PLoS Negl Trop Dis"},{"key":"8_CR5","doi-asserted-by":"publisher","first-page":"e1007706","DOI":"10.1371\/journal.ppat.1007706","volume":"15","author":"CV Filomatori","year":"2019","unstructured":"Filomatori CV, Bardossy ES, Merwaiss F et al (2019) RNA recombination at chikungunya virus 3\u2019UTR as an evolutionary mechanism that provides adaptability. PLoS Pathog 15:e1007706","journal-title":"PLoS Pathog"}],"container-title":["Methods in Molecular Biology","Chikungunya Virus"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5496-5_8","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T06:08:59Z","timestamp":1789366139000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5496-5_8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,15]]},"ISBN":["9781071654958","9781071654965"],"references-count":5,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5496-5_8","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,15]]},"assertion":[{"value":"15 September 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}