{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:12:43Z","timestamp":1788307963949,"version":"build-2803163510"},"publisher-location":"New York, NY","reference-count":28,"publisher":"Springer US","isbn-type":[{"value":"9781071654996","type":"print"},{"value":"9781071655009","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5500-9_11","type":"book-chapter","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:14:55Z","timestamp":1788304495000},"page":"127-137","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transposon Sequencing in Leptospira interrogans"],"prefix":"10.1007","author":[{"given":"James","family":"Matsunaga","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,2]]},"reference":[{"key":"11_CR1","first-page":"141","volume":"415","author":"M Picardeau","year":"2018","unstructured":"Picardeau M (2018) Toolbox of molecular techniques for studying Leptospira spp. Curr Top Microbiol Immunol 415:141\u2013162","journal-title":"Curr Top Microbiol Immunol"},{"key":"11_CR2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-37949-x","volume":"9","author":"LGV Fernandes","year":"2019","unstructured":"Fernandes LGV, Guaman LP, Vasconcellos SA, Heinemann MB, Picardeau M, Nascimento ALTO (2019) Gene silencing based on RNA-guided catalytically inactive Cas9 (dCas9): a new tool for genetic engineering in Leptospira. Sci Rep 9:1839","journal-title":"Sci Rep"},{"key":"11_CR3","doi-asserted-by":"publisher","first-page":"1768","DOI":"10.1038\/s41598-021-81400-7","volume":"11","author":"LGV Fernandes","year":"2021","unstructured":"Fernandes LGV, Hornsby RL, Nascimento ALTO, Nally JE (2021) Genetic manipulation of pathogenic Leptospira: CRISPR interference (CRISPRi)-mediated gene silencing and rapid mutant recovery at 37 \u00b0C. Sci Rep 11:1768","journal-title":"Sci Rep"},{"key":"11_CR4","doi-asserted-by":"publisher","first-page":"915382","DOI":"10.3389\/fmicb.2022.915382","volume":"13","author":"LGV Fernandes","year":"2022","unstructured":"Fernandes LGV, Nascimento ALTO (2022) A novel breakthrough in Leptospira spp. mutagenesis: knockout by combination of CRISPR\/Cas9 and non-homologous end-joining systems. Front Microbiol 13:915382","journal-title":"Front Microbiol"},{"key":"11_CR5","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01516-24","volume":"15","author":"LGV Fernandes","year":"2024","unstructured":"Fernandes LGV, Hamond C, Tibbs-Cortes BW, Putz EJ, Olsen SC, Palmer MV, Nally JE (2024) CRISPR-prime editing, a versatile genetic tool to create specific mutations with a single nucleotide resolution in Leptospira. mBio 15:e0151624","journal-title":"mBio"},{"key":"11_CR6","doi-asserted-by":"publisher","first-page":"810","DOI":"10.1128\/IAI.01293-08","volume":"77","author":"GL Murray","year":"2009","unstructured":"Murray GL, Morel V, Cerqueira GM, Croda J, Srikram A, Henry R, Ko AI, Dellagostin OA, Bulach DM, Sermswan RW, Adler B, Picardeau M (2009) Genome-wide transposon mutagenesis in pathogenic Leptospira species. Infect Immun 77:810\u2013816","journal-title":"Infect Immun"},{"key":"11_CR7","doi-asserted-by":"publisher","first-page":"3255","DOI":"10.1128\/JB.187.9.3255-3258.2005","volume":"187","author":"P Bourhy","year":"2005","unstructured":"Bourhy P, Louvel H, Saint Girons I, Picardeau M (2005) Random insertional mutagenesis of Leptospira interrogans, the agent of leptospirosis, using a mariner transposon. J Bacteriol 187:3255\u20133258","journal-title":"J Bacteriol"},{"key":"11_CR8","doi-asserted-by":"publisher","first-page":"3249","DOI":"10.1128\/JB.187.9.3249-3254.2005","volume":"187","author":"H Louvel","year":"2005","unstructured":"Louvel H, Saint Girons I, Picardeau M (2005) Isolation and characterization of FecA- and FeoB-mediated iron acquisition systems of the spirochete Leptospira biflexa by random insertional mutagenesis. J Bacteriol 187:3249\u20133254","journal-title":"J Bacteriol"},{"key":"11_CR9","doi-asserted-by":"publisher","first-page":"1601","DOI":"10.1099\/jmm.0.058586-0","volume":"62","author":"RA Marcsisin","year":"2013","unstructured":"Marcsisin RA, Bartpho T, Bulach DM, Srikram A, Sermswan RW, Adler B, Murray GL (2013) Use of a high-throughput screen to identify Leptospira mutants unable to colonize the carrier host or cause disease in the acute model of infection. J Med Microbiol 62:1601\u20131608","journal-title":"J Med Microbiol"},{"key":"11_CR10","doi-asserted-by":"publisher","first-page":"767","DOI":"10.1038\/nmeth.1377","volume":"6","author":"T Van Opijnen","year":"2009","unstructured":"Van Opijnen T, Bodi KL, Camilli A (2009) Tn-seq: high-throughput parallel sequencing for fitness and genetic interaction studies in microorganisms. Nat Methods 6:767\u2013772","journal-title":"Nat Methods"},{"key":"11_CR11","doi-asserted-by":"crossref","unstructured":"Van Opijnen T, Lazinski DW, Camilli A (2015). Genome-wide fitness and genetic interactions determined by Tn-seq, a high-throughput massively parallel sequencing method for microorganisms. CP Microbiol 36:1E.3.1\u20131E.3.24.","DOI":"10.1002\/9780471729259.mc01e03s36"},{"key":"11_CR12","doi-asserted-by":"publisher","first-page":"2347","DOI":"10.1128\/IAI.00266-13","volume":"81","author":"EB Troy","year":"2013","unstructured":"Troy EB, Lin T, Gao L, Lazinski DW, Camilli A, Norris SJ, Hu LT (2013) Understanding barriers to Borrelia burgdorferi dissemination during infection using massively parallel sequencing. Infect Immun 81:2347\u20132357","journal-title":"Infect Immun"},{"key":"11_CR13","doi-asserted-by":"publisher","first-page":"e0005117","DOI":"10.1371\/journal.pntd.0005117","volume":"10","author":"K Lourdault","year":"2016","unstructured":"Lourdault K, Matsunaga J, Haake DA (2016) High-throughput parallel sequencing to measure fitness of Leptospira interrogans transposon insertion mutants during acute infection. PLoS Negl Trop Dis 10:e0005117","journal-title":"PLoS Negl Trop Dis"},{"key":"11_CR14","doi-asserted-by":"crossref","unstructured":"Lourdault K, Matsunaga J, Evangelista KV, Haake DA (2017). High-throughput parallel sequencing to measure fitness of Leptospira interrogans transposon insertion mutants during golden Syrian hamster infection. J Vis Exp 130:56442","DOI":"10.3791\/56442-v"},{"key":"11_CR15","first-page":"45","volume":"26","author":"HC Ellinghausen","year":"1965","unstructured":"Ellinghausen HC, Mccullough WG (1965) Nutrition of Leptospira pomona and growth of 13 other serotypes: fractionation of oleic albumin complex and a medium of bovine albumin and polysorbate 80. Am J Vet Res 26:45\u201351","journal-title":"Am J Vet Res"},{"key":"11_CR16","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1128\/jb.94.1.27-31.1967","volume":"94","author":"RC Johnson","year":"1967","unstructured":"Johnson RC, Harris VG (1967) Differentiation of pathogenic and saprophytic leptospires. I. Growth at low temperatures. J Bacteriol 94:27\u201331","journal-title":"J Bacteriol"},{"key":"11_CR17","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1007\/978-1-61779-603-6_9","volume":"859","author":"L Slamti","year":"2012","unstructured":"Slamti L, Picardeau M (2012) Construction of a library of random mutants in the spirochete Leptospira biflexa using a mariner transposon. Methods Mol Biol 859:169\u2013176","journal-title":"Methods Mol Biol"},{"key":"11_CR18","doi-asserted-by":"publisher","first-page":"2105","DOI":"10.1128\/IAI.00094-16","volume":"84","author":"EA Wunder","year":"2016","unstructured":"Wunder EA, Figueira CP, Santos GR, Lourdault K, Matthias MA, Vinetz JM, Ramos E, Haake DA, Picardeau M, Dos Reis MG, Ko AI (2016) Real-time PCR reveals rapid dissemination of Leptospira interrogans after intraperitoneal and conjunctival inoculation of hamsters. Infect Immun 84:2105\u20132115","journal-title":"Infect Immun"},{"key":"11_CR19","doi-asserted-by":"publisher","first-page":"58","DOI":"10.3389\/fimmu.2017.00058","volume":"8","author":"M Gomes-Solecki","year":"2017","unstructured":"Gomes-Solecki M, Santecchia I, Werts C (2017) Animal models of leptospirosis: of mice and hamsters. Front Immunol 8:58","journal-title":"Front Immunol"},{"key":"11_CR20","first-page":"21","volume":"387","author":"CE Cameron","year":"2015","unstructured":"Cameron CE (2015) Leptospiral structure, physiology, and metabolism. Curr Top Microbiol Immunol 387:21\u201341","journal-title":"Curr Top Microbiol Immunol"},{"key":"11_CR21","doi-asserted-by":"publisher","first-page":"e97","DOI":"10.1371\/journal.ppat.0030097","volume":"3","author":"P Ristow","year":"2007","unstructured":"Ristow P, Bourhy P, McBride FWDC, Figueira CP, Huerre M, Ave P, Girons IS, Ko AI, Picardeau M (2007) The OmpA-like protein Loa22 is essential for leptospiral virulence. PLoS Pathog 3:e97","journal-title":"PLoS Pathog"},{"key":"11_CR22","doi-asserted-by":"publisher","first-page":"2542","DOI":"10.1128\/IAI.01803-14","volume":"82","author":"A Eshghi","year":"2014","unstructured":"Eshghi A, Becam J, Lambert A, Sismeiro O, Dillies M-A, Jagla B, Wunder EA, Ko AI, Coppee J-Y, Goarant C, Picardeau M (2014) A putative regulatory genetic locus modulates virulence in the pathogen Leptospira interrogans. Infect Immun 82:2542\u20132552","journal-title":"Infect Immun"},{"key":"11_CR23","doi-asserted-by":"publisher","first-page":"5826","DOI":"10.1128\/IAI.00989-08","volume":"76","author":"J Croda","year":"2008","unstructured":"Croda J, Figueira CP, Wunder EA, Santos CS, Reis MG, Ko AI, Picardeau M (2008) Targeted mutagenesis in pathogenic Leptospira species: disruption of the LigB gene does not affect virulence in animal models of leptospirosis. Infect Immun 76:5826\u20135833","journal-title":"Infect Immun"},{"key":"11_CR24","doi-asserted-by":"publisher","first-page":"2019","DOI":"10.1128\/IAI.00131-12","volume":"80","author":"A Lambert","year":"2012","unstructured":"Lambert A, Picardeau M, Haake DA, Sermswan RW, Srikram A, Adler B, Murray GA (2012) FlaA proteins in Leptospira interrogans are essential for motility and virulence but are not required for formation of the flagellum sheath. Infect Immun 80:2019\u20132025","journal-title":"Infect Immun"},{"key":"11_CR25","doi-asserted-by":"crossref","unstructured":"Haake DA (2006). Hamster model of leptospirosis. CP Microbiology 2","DOI":"10.1002\/9780471729259.mc12e02s02"},{"key":"11_CR26","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003307","volume":"8","author":"ML Coutinho","year":"2014","unstructured":"Coutinho ML, Matsunaga J, Wang L-C, De La Pe\u00f1a Moctezuma A, Lewis MS, Babbitt JT, Aleixo JAG, Haake DA (2014) Kinetics of Leptospira interrogans infection in hamsters after intradermal and subcutaneous challenge. PLoS Negl Trop Dis 8: e3307","journal-title":"PLoS Negl Trop Dis"},{"key":"11_CR27","doi-asserted-by":"publisher","first-page":"e0007950","DOI":"10.1371\/journal.pntd.0007950","volume":"14","author":"N Nair","year":"2020","unstructured":"Nair N, Guedes MS, Werts C, Gomes-Solecki M (2020) The route of infection with Leptospira interrogans serovar Copenhageni affects the kinetics of bacterial dissemination and kidney colonization. PLoS Negl Trop Dis 14:e0007950","journal-title":"PLoS Negl Trop Dis"},{"key":"11_CR28","doi-asserted-by":"publisher","first-page":"e0010191","DOI":"10.1371\/journal.pntd.0010191","volume":"16","author":"J Wang","year":"2022","unstructured":"Wang J, Zhang W, Jin Z, Ding Y, Zhang S, Wu D, Cao Y (2022) A lethal model of Leptospira infection in hamster nasal mucosa. PLoS Negl Trop Dis 16:e0010191","journal-title":"PLoS Negl Trop Dis"}],"container-title":["Methods in Molecular Biology","Leptospira spp."],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5500-9_11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:14:56Z","timestamp":1788304496000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5500-9_11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071654996","9781071655009"],"references-count":28,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5500-9_11","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"2 September 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}