{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:12:41Z","timestamp":1788307961090,"version":"build-2803163510"},"publisher-location":"New York, NY","reference-count":27,"publisher":"Springer US","isbn-type":[{"value":"9781071654996","type":"print"},{"value":"9781071655009","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5500-9_22","type":"book-chapter","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:14:57Z","timestamp":1788304497000},"page":"263-279","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cell Adhesion Assays to Investigate Leptospiral Protein Interactions with ECM, Cell Receptors, and Cultured Mammalian Cells: Methods for Understanding Host-Leptospira Interactions"],"prefix":"10.1007","author":[{"given":"Aline F.","family":"Teixeira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana L. T. O.","family":"Nascimento","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,2]]},"reference":[{"key":"22_CR1","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1007\/s00430-002-0119-0","volume":"191","author":"CR Hauck","year":"2002","unstructured":"Hauck CR (2002) Cell adhesion receptors \u2013 signaling capacity and exploitation by bacterial pathogens. Med Microbiol Immunol 191:55\u201362","journal-title":"Med Microbiol Immunol"},{"key":"22_CR2","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1042\/bst0280311","volume":"28","author":"MJ Humphries","year":"2000","unstructured":"Humphries MJ (2000) Integrin structure. Biochem Soc Trans 28:311\u2013339","journal-title":"Biochem Soc Trans"},{"key":"22_CR3","doi-asserted-by":"publisher","first-page":"775","DOI":"10.1038\/nature01603","volume":"422","author":"S Gruenheid","year":"2003","unstructured":"Gruenheid S, Finlay BB (2003) Microbial pathogenesis and cytoskeletal function. Nature 422:775\u2013781","journal-title":"Nature"},{"key":"22_CR4","doi-asserted-by":"publisher","first-page":"18149","DOI":"10.3390\/ijms160818149","volume":"16","author":"AA Khalili","year":"2015","unstructured":"Khalili AA, Ahmad MR (2015) A review of cell adhesion studies for biomedical and biological applications. Int J Mol Sci 16:18149\u201318184","journal-title":"Int J Mol Sci"},{"key":"22_CR5","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1016\/j.cell.2006.02.012","volume":"124","author":"J Pizarro-Cerd\u00e1","year":"2006","unstructured":"Pizarro-Cerd\u00e1 J, Cossart P (2006) Bacterial adhesion and entry into host cells. Cell 124:715\u2013727","journal-title":"Cell"},{"key":"22_CR6","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1111\/imm.12972","volume":"155","author":"H Tomlin","year":"2018","unstructured":"Tomlin H, Piccinini AM (2018) A complex interplay between the extracellular matrix and the innate immune response to microbial pathogens. Immunology 155:186\u2013201","journal-title":"Immunology"},{"key":"22_CR7","doi-asserted-by":"publisher","first-page":"6850","DOI":"10.1111\/febs.15776","volume":"288","author":"NK Karamanos","year":"2021","unstructured":"Karamanos NK et al (2021) A guide to the composition and functions of the extracellular matrix. FEBS J 288:6850\u20136912","journal-title":"FEBS J"},{"key":"22_CR8","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1007\/s00441-009-0821-y","volume":"339","author":"L Schaefer","year":"2010","unstructured":"Schaefer L, Schaefer RM (2010) Proteoglycans: from structural compounds to signaling molecules. Cell Tissue Res 339:237\u2013246","journal-title":"Cell Tissue Res"},{"key":"22_CR9","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1111\/1574-6968.12349","volume":"352","author":"ML Vieira","year":"2014","unstructured":"Vieira ML et al (2014) Leptospiral extracellular matrix adhesins as mediators of pathogen-host interactions. FEMS Microbiol Lett 352:129\u2013139","journal-title":"FEMS Microbiol Lett"},{"key":"22_CR10","doi-asserted-by":"publisher","first-page":"6356","DOI":"10.1128\/IAI.00460-06","volume":"74","author":"AS Barbosa","year":"2006","unstructured":"Barbosa AS et al (2006) A newly identified leptospiral adhesin mediates attachment to laminin. Infect Immun 74:6356\u20136364","journal-title":"Infect Immun"},{"key":"22_CR11","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1099\/mic.0.072074-0","volume":"160","author":"LGV Fernandes","year":"2014","unstructured":"Fernandes LGV et al (2014) Functional and immunological evaluation of two novel proteins of Leptospira spp. Microbiology 160:149\u2013164","journal-title":"Microbiology"},{"key":"22_CR12","doi-asserted-by":"publisher","first-page":"e21962","DOI":"10.1371\/journal.pone.0021962","volume":"6","author":"R Oliveira","year":"2011","unstructured":"Oliveira R et al (2011) Characterization of novel OmpA-like protein of Leptospira interrogans that binds extracellular matrix molecules and plasminogen. PLoS One 6:e21962","journal-title":"PLoS One"},{"key":"22_CR13","doi-asserted-by":"publisher","first-page":"e0122762","DOI":"10.1371\/journal.pone.0122762","volume":"10","author":"AF Teixeira","year":"2015","unstructured":"Teixeira AF et al (2015) Features of two new proteins with OmpA-like domains identified in the genome sequences of Leptospira interrogans. PLoS One 10:e0122762","journal-title":"PLoS One"},{"key":"22_CR14","doi-asserted-by":"crossref","unstructured":"Silva LP et al. (2016) Evaluation of two novel leptospiral proteins for their interaction with human host components. Pathog Dis 74(5):ftw040","DOI":"10.1093\/femspd\/ftw040"},{"key":"22_CR15","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1016\/j.ijmm.2017.05.006","volume":"307","author":"PRM Pereira","year":"2017","unstructured":"Pereira PRM et al (2017) Multifunctional and redundant roles of Leptospira interrogans proteins in bacterial-adhesion and fibrin clotting inhibition. Int J Med Microbiol 307:297\u2013310","journal-title":"Int J Med Microbiol"},{"key":"22_CR16","doi-asserted-by":"publisher","first-page":"163","DOI":"10.3389\/fcimb.2017.00163","volume":"7","author":"CL Hsieh","year":"2017","unstructured":"Hsieh CL et al (2017) Immunoglobulin-like protein B interacts with the 20th exon of human tropoelastin contributing to. Front Cell Infect Microbiol 7:163","journal-title":"Front Cell Infect Microbiol"},{"key":"22_CR17","doi-asserted-by":"publisher","first-page":"e11301","DOI":"10.1371\/journal.pone.0011301","volume":"5","author":"YP Lin","year":"2010","unstructured":"Lin YP et al (2010) The terminal immunoglobulin-like repeats of LigA and LigB of Leptospira enhance their binding to gelatin binding domain of fibronectin and host cells. PLoS One 5:e11301","journal-title":"PLoS One"},{"key":"22_CR18","doi-asserted-by":"publisher","first-page":"e1188","DOI":"10.1371\/journal.pone.0001188","volume":"2","author":"B Stevenson","year":"2007","unstructured":"Stevenson B et al (2007) Leptospira interrogans endostatin-like outer membrane proteins bind host fibronectin, laminin and regulators of complement. PLoS One 2:e1188","journal-title":"PLoS One"},{"key":"22_CR19","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1016\/j.jmii.2018.12.012","volume":"53","author":"AD Rossini","year":"2020","unstructured":"Rossini AD et al (2020) Identification of a novel protein in the genome sequences of Leptospira interrogans with the ability to interact with host's components. J Microbiol Immunol Infect 53:163\u2013175","journal-title":"J Microbiol Immunol Infect"},{"key":"22_CR20","doi-asserted-by":"publisher","first-page":"586","DOI":"10.1111\/1348-0421.12406","volume":"60","author":"MR Cosate","year":"2016","unstructured":"Cosate MR et al (2016) Mammalian cell entry (Mce) protein of Leptospira interrogans binds extracellular matrix components, plasminogen and \u03b22 integrin. Microbiol Immunol 60:586\u2013598","journal-title":"Microbiol Immunol"},{"key":"22_CR21","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1099\/mic.0.000411","volume":"163","author":"JM Figueredo","year":"2017","unstructured":"Figueredo JM et al (2017) Characterization of two new putative adhesins of Leptospira interrogans. Microbiology 163:37\u201351","journal-title":"Microbiology"},{"key":"22_CR22","doi-asserted-by":"crossref","unstructured":"Cavenague MF et al (2023) LIC12254 is a leptospiral protein that interacts with integrins via the RGD motif. Trop Med Infect Dis 8(5):249","DOI":"10.3390\/tropicalmed8050249"},{"key":"22_CR23","doi-asserted-by":"publisher","first-page":"e3215","DOI":"10.1371\/journal.pntd.0003215","volume":"8","author":"KV Evangelista","year":"2014","unstructured":"Evangelista KV et al (2014) Identification of cell-binding adhesins of Leptospira interrogans. PLoS Negl Trop Dis 8:e3215","journal-title":"PLoS Negl Trop Dis"},{"key":"22_CR24","doi-asserted-by":"crossref","unstructured":"Takahashi MB, Teixeira AF, Nascimento ALTO (2022) Host cell binding mediated by Leptospira interrogans adhesins. Int J Mol Sci 23(24):15550","DOI":"10.3390\/ijms232415550"},{"key":"22_CR25","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1002\/2211-5463.13942","volume":"15","author":"IRM Silva","year":"2025","unstructured":"Silva IRM et al (2025) Evaluation of binding activities of a putative lipoprotein LIC_13355 of Leptospira spp. FEBS Open Bio 15:447\u2013461","journal-title":"FEBS Open Bio"},{"key":"22_CR26","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1007\/978-1-59745-413-1_14","volume":"522","author":"MJ Humphries","year":"2009","unstructured":"Humphries MJ (2009) Cell adhesion assays. Methods Mol Biol 522:203\u2013210","journal-title":"Methods Mol Biol"},{"key":"22_CR27","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1128\/iai.58.3.581-585.1990","volume":"58","author":"DD Thomas","year":"1990","unstructured":"Thomas DD, Higbie LM (1990) In vitro association of leptospires with host cells. Infect Immun 58:581\u2013585","journal-title":"Infect Immun"}],"container-title":["Methods in Molecular Biology","Leptospira spp."],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5500-9_22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:14:58Z","timestamp":1788304498000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5500-9_22"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071654996","9781071655009"],"references-count":27,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5500-9_22","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"2 September 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}