{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:18:20Z","timestamp":1788304700727,"version":"build-2803163510"},"publisher-location":"New York, NY","reference-count":33,"publisher":"Springer US","isbn-type":[{"value":"9781071655146","type":"print"},{"value":"9781071655153","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5515-3_10","type":"book-chapter","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:04:36Z","timestamp":1788303876000},"page":"147-172","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Experimental Tools and Techniques for Studying Microtubule Nucleation at the Centrosome and the Golgi Apparatus"],"prefix":"10.1007","author":[{"given":"Chiara","family":"Marcozzi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria P.","family":"Gavil\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa M.","family":"R\u00edos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,2]]},"reference":[{"key":"10_CR1","unstructured":"Alberts B et al (2022) Molecular biology of the cell, 7th edn. W. W. Norton & Company"},{"issue":"8","key":"10_CR2","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1038\/s41580-022-00473-y","volume":"23","author":"A Akhmanova","year":"2022","unstructured":"Akhmanova A, Kapitein LC (2022) Mechanisms of microtubule organization in differentiated animal cells. Nat Rev Mol Cell Biol 23(8):541\u2013558. https:\/\/doi.org\/10.1038\/s41580-022-00473-y","journal-title":"Nat Rev Mol Cell Biol"},{"key":"10_CR3","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1016\/j.sbi.2020.11.002","volume":"66","author":"M Bornens","year":"2021","unstructured":"Bornens M (2021) Centrosome organization and functions. Curr Opin Struct Biol 66:199\u2013206. https:\/\/doi.org\/10.1016\/j.sbi.2020.11.002","journal-title":"Curr Opin Struct Biol"},{"issue":"7","key":"10_CR4","doi-asserted-by":"publisher","first-page":"2047","DOI":"10.1091\/mbc.12.7.2047","volume":"12","author":"K Chabin-Brion","year":"2001","unstructured":"Chabin-Brion K, Marceiller J, Perez F, Settegrana C, Drechou A, Durand G, Pous C (2001) The Golgi complex is a microtubule-organizing organelle. Mol Biol Cell 12(7):2047\u20132060. https:\/\/doi.org\/10.1091\/mbc.12.7.2047","journal-title":"Mol Biol Cell"},{"issue":"6","key":"10_CR5","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1016\/j.devcel.2007.04.002","volume":"12","author":"A Efimov","year":"2007","unstructured":"Efimov A, Kharitonov A, Efimova N, Loncarek J, Miller PM, Andreyeva N, Gleeson P, Galjart N, Maia AR, McLeod IX, Yates JR 3rd, Maiato H, Khodjakov A, Akhmanova A, Kaverina I (2007) Asymmetric CLASP-dependent nucleation of noncentrosomal microtubules at the trans-Golgi network. Dev Cell 12(6):917\u2013930. https:\/\/doi.org\/10.1016\/j.devcel.2007.04.002","journal-title":"Dev Cell"},{"key":"10_CR6","doi-asserted-by":"publisher","unstructured":"Rios RM (2014) The centrosome-Golgi apparatus nexus. Philos Trans R Soc Lond B Biol Sci 369(1650):20130452. https:\/\/doi.org\/10.1098\/rstb.2013.0462","DOI":"10.1098\/rstb.2013.0462"},{"issue":"10","key":"10_CR7","doi-asserted-by":"publisher","first-page":"R506","DOI":"10.1016\/j.cub.2021.01.042","volume":"31","author":"MD Sallee","year":"2021","unstructured":"Sallee MD, Feldman JL (2021) Microtubule organization across cell types and states. Curr Biol 31(10):R506\u2013R511. https:\/\/doi.org\/10.1016\/j.cub.2021.01.042","journal-title":"Curr Biol"},{"issue":"11","key":"10_CR8","doi-asserted-by":"publisher","first-page":"1148","DOI":"10.1038\/ncb2591","volume":"14","author":"S Lawo","year":"2012","unstructured":"Lawo S, Hasegan M, Gupta GD, Pelletier L (2012) Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material. Nat Cell Biol 14(11):1148\u20131158. https:\/\/doi.org\/10.1038\/ncb2591","journal-title":"Nat Cell Biol"},{"key":"10_CR9","doi-asserted-by":"publisher","unstructured":"Woodruff JB, Wueseke O, Hyman AA (2014) Pericentriolar material structure and dynamics. Philos Trans R Soc Lond B Biol Sci 369(1650). https:\/\/doi.org\/10.1098\/rstb.2013.0459","DOI":"10.1098\/rstb.2013.0459"},{"issue":"1","key":"10_CR10","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/s0955-0674(01)00290-3","volume":"14","author":"M Bornens","year":"2002","unstructured":"Bornens M (2002) Centrosome composition and microtubule anchoring mechanisms. Curr Opin Cell Biol 14(1):25\u201334. https:\/\/doi.org\/10.1016\/s0955-0674(01)00290-3","journal-title":"Curr Opin Cell Biol"},{"key":"10_CR11","doi-asserted-by":"publisher","unstructured":"Rivero S, Cardenas J, Bornens M, Rios RM (2009) Microtubule nucleation at the cis-side of the Golgi apparatus requires AKAP450 and GM130. EMBO J 28(8):1016\u201328. https:\/\/doi.org\/10.1038\/emboj.2009.47","DOI":"10.1038\/emboj.2009.47"},{"key":"10_CR12","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1016\/j.sbi.2020.09.013","volume":"66","author":"KS Lee","year":"2021","unstructured":"Lee KS, Park JE, Ahn JI, Zeng Y (2021) Constructing PCM with architecturally distinct higher-order assemblies. Curr Opin Struct Biol 66:66\u201373. https:\/\/doi.org\/10.1016\/j.sbi.2020.09.013","journal-title":"Curr Opin Struct Biol"},{"key":"10_CR13","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.semcdb.2021.03.020","volume":"117","author":"A Vasquez-Limeta","year":"2021","unstructured":"Vasquez-Limeta A, Loncarek J (2021) Human centrosome organization and function in interphase and mitosis. Semin Cell Dev Biol 117:30\u201341. https:\/\/doi.org\/10.1016\/j.semcdb.2021.03.020","journal-title":"Semin Cell Dev Biol"},{"issue":"1","key":"10_CR14","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1002\/cm.21079","volume":"70","author":"AR Maia","year":"2013","unstructured":"Maia AR, Zhu X, Miller P, Gu G, Maiato H, Kaverina I (2013) Modulation of Golgi-associated microtubule nucleation throughout the cell cycle. Cytoskeleton (Hoboken) 70(1):32\u201343. https:\/\/doi.org\/10.1002\/cm.21079","journal-title":"Cytoskeleton (Hoboken)"},{"issue":"3","key":"10_CR15","doi-asserted-by":"publisher","first-page":"492","DOI":"10.1016\/j.neuron.2015.05.046","volume":"87","author":"LC Kapitein","year":"2015","unstructured":"Kapitein LC, Hoogenraad CC (2015) Building the neuronal microtubule cytoskeleton. Neuron 87(3):492\u2013506. https:\/\/doi.org\/10.1016\/j.neuron.2015.05.046","journal-title":"Neuron"},{"key":"10_CR16","doi-asserted-by":"publisher","unstructured":"Lucas L, Cooper TA (2023) Insights into cell-specific functions of microtubules in skeletal muscle development and homeostasis. Int J Mol Sci 24(3):2903. https:\/\/doi.org\/10.3390\/ijms24032903","DOI":"10.3390\/ijms24032903"},{"key":"10_CR17","doi-asserted-by":"publisher","first-page":"915206","DOI":"10.3389\/fcell.2022.915206","volume":"10","author":"KM Bracey","year":"2022","unstructured":"Bracey KM, Gu G, Kaverina I (2022) Microtubules in pancreatic beta Cells: convoluted roadways toward precision. Front Cell Dev Biol 10:915206. https:\/\/doi.org\/10.3389\/fcell.2022.915206","journal-title":"Front Cell Dev Biol"},{"issue":"11","key":"10_CR18","doi-asserted-by":"publisher","first-page":"702","DOI":"10.1038\/nrm.2017.75","volume":"18","author":"J Roostalu","year":"2017","unstructured":"Roostalu J, Surrey T (2017) Microtubule nucleation: beyond the template. Nat Rev Mol Cell Biol 18(11):702\u2013710. https:\/\/doi.org\/10.1038\/nrm.2017.75","journal-title":"Nat Rev Mol Cell Biol"},{"key":"10_CR19","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1016\/j.ceb.2020.10.004","volume":"68","author":"P Liu","year":"2021","unstructured":"Liu P, Wurtz M, Zupa E, Pfeffer S, Schiebel E (2021) Microtubule nucleation: the waltz between gamma-tubulin ring complex and associated proteins. Curr Opin Cell Biol 68:124\u2013131. https:\/\/doi.org\/10.1016\/j.ceb.2020.10.004","journal-title":"Curr Opin Cell Biol"},{"issue":"1","key":"10_CR20","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1016\/j.devcel.2016.08.009","volume":"39","author":"J Wu","year":"2016","unstructured":"Wu J, de Heus C, Liu Q, Bouchet BP, Noordstra I, Jiang K, Hua S, Martin M, Yang C, Grigoriev I, Katrukha EA, Altelaar AFM, Hoogenraad CC, Qi RZ, Klumperman J, Akhmanova A (2016) Molecular pathway of microtubule organization at the Golgi apparatus. Dev Cell 39(1):44\u201360. https:\/\/doi.org\/10.1016\/j.devcel.2016.08.009","journal-title":"Dev Cell"},{"key":"10_CR21","doi-asserted-by":"publisher","unstructured":"Gavilan MP, Gandolfo P, Balestra FR, Arias F, Bornens M, Rios RM (2018) The dual role of the centrosome in organizing the microtubule network in interphase. EMBO Rep 19(11):e45942. https:\/\/doi.org\/10.15252\/embr.201845942","DOI":"10.15252\/embr.201845942"},{"issue":"6","key":"10_CR22","doi-asserted-by":"publisher","first-page":"1089","DOI":"10.1083\/jcb.201007030","volume":"191","author":"YK Choi","year":"2010","unstructured":"Choi YK, Liu P, Sze SK, Dai C, Qi RZ (2010) CDK5RAP2 stimulates microtubule nucleation by the gamma-tubulin ring complex. J Cell Biol 191(6):1089\u20131095. https:\/\/doi.org\/10.1083\/jcb.201007030","journal-title":"J Cell Biol"},{"issue":"1","key":"10_CR23","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-35955-w","volume":"14","author":"A Ali","year":"2023","unstructured":"Ali A, Vineethakumari C, Lacasa C, Luders J (2023) Microtubule nucleation and gammaTuRC centrosome localization in interphase cells require ch-TOG. Nat Commun 14(1):289. https:\/\/doi.org\/10.1038\/s41467-023-35955-w","journal-title":"Nat Commun"},{"issue":"3","key":"10_CR24","doi-asserted-by":"publisher","first-page":"e1002087","DOI":"10.1371\/journal.pbio.1002087","volume":"13","author":"MP Gavilan","year":"2015","unstructured":"Gavilan MP, Arjona M, Zurbano A, Formstecher E, Martinez-Morales JR, Bornens M, Rios RM (2015) Alpha-catenin-dependent recruitment of the centrosomal protein CAP350 to adherens junctions allows epithelial cells to acquire a columnar shape. PLoS Biol 13(3):e1002087. https:\/\/doi.org\/10.1371\/journal.pbio.1002087","journal-title":"PLoS Biol"},{"issue":"1","key":"10_CR25","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1083\/jcb.145.1.83","volume":"145","author":"C Infante","year":"1999","unstructured":"Infante C, Ramos-Morales F, Fedriani C, Bornens M, Rios RM (1999) GMAP-210, A cis-Golgi network-associated protein, is a minus end microtubule-binding protein. J Cell Biol 145(1):83\u201398. https:\/\/doi.org\/10.1083\/jcb.145.1.83","journal-title":"J Cell Biol"},{"issue":"2","key":"10_CR26","doi-asserted-by":"publisher","first-page":"100407","DOI":"10.1016\/j.xpro.2021.100407","volume":"2","author":"S Ghetti","year":"2021","unstructured":"Ghetti S, Burigotto M, Mattivi A, Magnani G, Casini A, Bianchi A, Cereseto A, Fava LL (2021) CRISPR\/Cas9 ribonucleoprotein-mediated knockin generation in hTERT-RPE1 cells. STAR Protoc 2(2):100407. https:\/\/doi.org\/10.1016\/j.xpro.2021.100407","journal-title":"STAR Protoc"},{"key":"10_CR27","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1016\/B978-0-12-417164-0.00024-0","volume":"118","author":"AD Grimaldi","year":"2013","unstructured":"Grimaldi AD, Fomicheva M, Kaverina I (2013) Ice recovery assay for detection of Golgi-derived microtubules. Methods Cell Biol 118:401\u2013415. https:\/\/doi.org\/10.1016\/B978-0-12-417164-0.00024-0","journal-title":"Methods Cell Biol"},{"issue":"11","key":"10_CR28","doi-asserted-by":"publisher","first-page":"1154","DOI":"10.1091\/mbc.E19-11-0634","volume":"31","author":"G Li","year":"2020","unstructured":"Li G, Moore JK (2020) Microtubule dynamics at low temperature: evidence that tubulin recycling limits assembly. Mol Biol Cell 31(11):1154\u20131166. https:\/\/doi.org\/10.1091\/mbc.E19-11-0634","journal-title":"Mol Biol Cell"},{"key":"10_CR29","doi-asserted-by":"publisher","unstructured":"Frye K, Renda F, Fomicheva M, Zhu X, Gong L, Khodjakov A, Kaverina I (2020) Cell cycle-dependent dynamics of the Golgi-Centrosome Association in motile cells. Cells 9(5):1069. https:\/\/doi.org\/10.3390\/cells9051069","DOI":"10.3390\/cells9051069"},{"issue":"5","key":"10_CR30","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1083\/jcb.201011014","volume":"193","author":"L Hurtado","year":"2011","unstructured":"Hurtado L, Caballero C, Gavilan MP, Cardenas J, Bornens M, Rios RM (2011) Disconnecting the Golgi ribbon from the centrosome prevents directional cell migration and ciliogenesis. J Cell Biol 193(5):917\u2013933. https:\/\/doi.org\/10.1083\/jcb.201011014","journal-title":"J Cell Biol"},{"key":"10_CR31","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1007\/978-1-0716-0219-5_12","volume":"2101","author":"RJ Thoppil","year":"2020","unstructured":"Thoppil RJ, Sanders A, Kaverina I (2020) Detection of microtubule nucleation hotspots at the Golgi. Methods Mol Biol 2101:179\u2013189. https:\/\/doi.org\/10.1007\/978-1-0716-0219-5_12","journal-title":"Methods Mol Biol"},{"key":"10_CR32","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1007\/978-1-0716-0219-5_11","volume":"2101","author":"A Ezquerra","year":"2020","unstructured":"Ezquerra A, Viais R, Luders J (2020) Assaying microtubule nucleation. Methods Mol Biol 2101:163\u2013178. https:\/\/doi.org\/10.1007\/978-1-0716-0219-5_11","journal-title":"Methods Mol Biol"},{"issue":"10","key":"10_CR33","doi-asserted-by":"publisher","first-page":"100597","DOI":"10.1016\/j.crmeth.2023.100597","volume":"3","author":"JA Andres-San Roman","year":"2023","unstructured":"Andres-San Roman JA, Gordillo-Vazquez C, Franco-Barranco D, Morato L, Fernandez-Espartero CH, Baonza G, Tagua A, Vicente-Munuera P, Palacios AM, Gavilan MP, Martin-Belmonte F, Annese V, Gomez-Galvez P, Arganda-Carreras I, Escudero LM (2023) CartoCell, a high-content pipeline for 3D image analysis, unveils cell morphology patterns in epithelia. Cell Rep Methods 3(10):100597. https:\/\/doi.org\/10.1016\/j.crmeth.2023.100597","journal-title":"Cell Rep Methods"}],"container-title":["Methods in Molecular Biology","The Cytoskeleton"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5515-3_10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:04:37Z","timestamp":1788303877000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5515-3_10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071655146","9781071655153"],"references-count":33,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5515-3_10","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"2 September 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}