{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T07:17:58Z","timestamp":1787901478616,"version":"build-2784847793"},"publisher-location":"New York, NY","reference-count":21,"publisher":"Springer US","isbn-type":[{"value":"9781071655269","type":"print"},{"value":"9781071655276","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-5527-6_16","type":"book-chapter","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T06:44:43Z","timestamp":1787899483000},"page":"255-267","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protein Transport Studied by a Model Asymmetric Membrane Army Arranged in a Dimple Chip"],"prefix":"10.1007","author":[{"given":"Jing","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,29]]},"reference":[{"key":"16_CR1","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/0079-6107(84)90014-2","volume":"43","author":"G Benga","year":"1984","unstructured":"Benga G, Holmes RP (1984) Interactions between components in biological membranes and their implications for membrane function. Prog Biophys Mol Biol 43:195\u2013257","journal-title":"Prog Biophys Mol Biol"},{"key":"16_CR2","doi-asserted-by":"publisher","first-page":"47","DOI":"10.3109\/10409238309102790","volume":"14","author":"KE Bloch","year":"1983","unstructured":"Bloch KE (1983) Sterol, structure and membrane function. Crit Rev Biochem 14:47\u201392","journal-title":"Crit Rev Biochem"},{"key":"16_CR3","doi-asserted-by":"publisher","first-page":"4169","DOI":"10.1021\/acs.langmuir.6b00511","volume":"32","author":"T Noyhouzer","year":"2016","unstructured":"Noyhouzer T, L\u2019Homme C, Beaulieu I, Mazurkiewicz S, Kuss S, Kraatz H-B, Canesi S, Mauzeroll J (2016) Ferrocene-modified phospholipid: an innovative precursor for redox-triggered drug delivery vesicles selective to cancer cells. Langmuir 32:4169\u20134178","journal-title":"Langmuir"},{"key":"16_CR4","doi-asserted-by":"publisher","first-page":"3836","DOI":"10.1021\/acssynbio.2c00413","volume":"11","author":"A Miwa","year":"2022","unstructured":"Miwa A, Kamiya K (2022) Control of enzyme reaction initiation inside giant unilamellar vesicles by the cell-penetrating peptide-mediated translocation of cargo proteins. ACS Synth Biol 11:3836\u20133846","journal-title":"ACS Synth Biol"},{"key":"16_CR5","doi-asserted-by":"publisher","unstructured":"Keller H, Worch R, Schwille P (2013) Model Membrane Systems. In: Williams MA, Daviter T (eds) Protein-Ligand Interactions. Methods in Molecular Biology, vol 1008. Humana Press, Totowa, NJ. Print ISBN: 978-1-62703-397-8; Online ISBN: 978-1-62703-398-5. https:\/\/doi.org\/10.1007\/978-1-62703-398-5_15","DOI":"10.1007\/978-1-62703-398-5_15"},{"key":"16_CR6","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/j.cell.2008.11.013","volume":"136","author":"S Saksena","year":"2009","unstructured":"Saksena S, Wahlman J, Teis D, Johnson AE, Emr SD (2009) Functional reconstitution of ESCRT-III assembly and disassembly. Cell 136:97\u2013109","journal-title":"Cell"},{"key":"16_CR7","doi-asserted-by":"publisher","first-page":"4093","DOI":"10.1021\/bi00517a023","volume":"20","author":"DK Struck","year":"1981","unstructured":"Struck DK, Hoekstra D, Pagano RE (1981) Use of resonance energy transfer to monitor membrane fusion. Biochemistry 20:4093\u20134099","journal-title":"Biochemistry"},{"key":"16_CR8","doi-asserted-by":"publisher","first-page":"1167","DOI":"10.1161\/01.CIR.26.5.1167","volume":"26","author":"P Mueller","year":"1962","unstructured":"Mueller P, Rudin DO, Tien HT, Wescott WC (1962) Reconstitution of excitable cell membrane structure in vitro. Circulation 26:1167\u20131171","journal-title":"Circulation"},{"key":"16_CR9","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-31925-1","volume":"8","author":"V Yeh","year":"2018","unstructured":"Yeh V, Lee TY, Chen CW, Kuo PC, Shiue J, Chu LK, Yu TY (2018) Highly efficient transfer of 7TM membrane protein from native membrane to covalently circularized nanodisc. Sci Rep 8:13501","journal-title":"Sci Rep"},{"key":"16_CR10","doi-asserted-by":"publisher","first-page":"102844","DOI":"10.1016\/j.sbi.2024.102844","volume":"87","author":"IG Denisov","year":"2024","unstructured":"Denisov IG, Sligar SG (2024) Nanodiscs for the study of membrane proteins. Curr Opin Struct Biol 87:102844","journal-title":"Curr Opin Struct Biol"},{"key":"16_CR11","doi-asserted-by":"publisher","first-page":"1191","DOI":"10.1039\/B808893D","volume":"4","author":"H Bayley","year":"2008","unstructured":"Bayley H, Cronin B, Heron A, Holden MA, Hwang WL, Syeda R, Thompson J, Wallace M (2008) Droplet interface bilayers. Mol BioSyst 4:1191\u20131120","journal-title":"Mol BioSyst"},{"key":"16_CR12","doi-asserted-by":"publisher","first-page":"15543","DOI":"10.1021\/ja804968g","volume":"130","author":"R Syeda","year":"2008","unstructured":"Syeda R, Holden MA, Hwang WL, Bayley H (2008) Screening blockers against a potassium channel with a droplet interface bilayer array. J Am Chem Soc 130:15543\u201315548","journal-title":"J Am Chem Soc"},{"key":"16_CR13","doi-asserted-by":"publisher","first-page":"8650","DOI":"10.1021\/ja072292a","volume":"129","author":"MA Holden","year":"2007","unstructured":"Holden MA, Needham D, Bayley H (2007) Functional bionetworks from nanoliter water droplets. J Am Chem Soc 129:8650\u20138655","journal-title":"J Am Chem Soc"},{"key":"16_CR14","doi-asserted-by":"publisher","first-page":"5878","DOI":"10.1021\/ja802089s","volume":"130","author":"WL Hwang","year":"2008","unstructured":"Hwang WL, Chen M, Cronin B, Holden MA, Bayley H (2008) Asymmetric droplet interface bilayers. J Am Chem Soc 130:5878\u20135879","journal-title":"J Am Chem Soc"},{"key":"16_CR15","doi-asserted-by":"publisher","first-page":"15818","DOI":"10.1021\/ja2046342","volume":"133","author":"J Huang","year":"2011","unstructured":"Huang J, Lein M, Gunderson C, Holden MA (2011) Direct quantitation of peptide-mediated protein transport across a droplet\u2013interface bilayer. J Am Chem Soc 133:15818\u201315821","journal-title":"J Am Chem Soc"},{"key":"16_CR16","doi-asserted-by":"publisher","first-page":"1173","DOI":"10.1038\/nbt1201-1173","volume":"19","author":"MC Morris","year":"2001","unstructured":"Morris MC, Depollier J, Mery J, Heitz F, Divita G (2001) A peptide carrier for the delivery of biologically active proteins into mammalian cells. Nat Biotechnol 19:1173\u20131176","journal-title":"Nat Biotechnol"},{"key":"16_CR17","doi-asserted-by":"publisher","first-page":"12369","DOI":"10.1021\/acs.analchem.7b03421","volume":"89","author":"X Li","year":"2017","unstructured":"Li X, Huang J, Holden MA, Chen M (2017) Peptide-mediated membrane transport of macromolecular cargo driven by membrane asymmetry. Anal Chem 89:12369\u201312374","journal-title":"Anal Chem"},{"key":"16_CR18","doi-asserted-by":"publisher","first-page":"183415","DOI":"10.1016\/j.bbamem.2020.183415","volume":"1862","author":"P Gehan","year":"2020","unstructured":"Gehan P, Kulifaj S, Soule P, Bodin JB, Amoura M, Walrant A, Sagan S, Thiam AR, Ngo K, Vivier V, Cribier S, Rodriguez N (2020) Penetratin translocation mechanism through asymmetric droplet interface bilayers. Biochim Biophys Acta Biomembr 1862:183415","journal-title":"Biochim Biophys Acta Biomembr"},{"key":"16_CR19","doi-asserted-by":"publisher","first-page":"5682","DOI":"10.1021\/acs.langmuir.2c00269","volume":"38","author":"V Faugeras","year":"2022","unstructured":"Faugeras V, Duclos O, Bazile D, Thiam AR (2022) Impact of cyclization and methylation on peptide penetration through droplet interface bilayers. Langmuir 38:5682\u20135691","journal-title":"Langmuir"},{"key":"16_CR20","doi-asserted-by":"publisher","first-page":"198","DOI":"10.1016\/S0005-2736(00)00221-2","volume":"1467","author":"WC Hung","year":"2000","unstructured":"Hung WC, Chen FY, Huang HW (2000) Order\u2013disorder transition in bilayers of diphytanoyl phosphatidylcholine. Biochim Biophys Acta Biomembr 1467:198\u2013206","journal-title":"Biochim Biophys Acta Biomembr"},{"key":"16_CR21","doi-asserted-by":"publisher","first-page":"5970","DOI":"10.1039\/D0SM00667J","volume":"16","author":"V Faugeras","year":"2020","unstructured":"Faugeras V, Duclos O, Bazile D, Thiam AR (2020) Membrane determinants for the passive translocation of analytes through droplet interface bilayers. Soft Matter 16:5970\u20135980","journal-title":"Soft Matter"}],"container-title":["Methods in Molecular Biology","Nanopore Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5527-6_16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T06:44:44Z","timestamp":1787899484000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5527-6_16"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9781071655269","9781071655276"],"references-count":21,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5527-6_16","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"29 August 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}