{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T07:00:23Z","timestamp":1790060423979,"version":"4.0.1"},"publisher-location":"New York, NY","reference-count":21,"publisher":"Springer US","isbn-type":[{"value":"9781071655306","type":"print"},{"value":"9781071655313","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2027]]},"DOI":"10.1007\/978-1-0716-5531-3_20","type":"book-chapter","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T05:33:45Z","timestamp":1790055225000},"page":"273-291","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Disruption of Chlamydia trachomatis Essential Genes Using DOPE (Dependence on Plasmid-Mediated Expression)"],"prefix":"10.1007","author":[{"given":"Yuxuan","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huizhou","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,23]]},"reference":[{"issue":"9","key":"20_CR1","doi-asserted-by":"publisher","first-page":"e1002258","DOI":"10.1371\/journal.ppat.1002258","volume":"7","author":"Y Wang","year":"2011","unstructured":"Wang Y, Kahane S, Cutcliffe LT, Skilton RJ, Lambden PR, Clarke IN (2011) Development of a transformation system for Chlamydia trachomatis: restoration of glycogen biosynthesis by acquisition of a plasmid shuttle vector. PLoS Pathog 7(9):e1002258. https:\/\/doi.org\/10.1371\/journal.ppat.1002258","journal-title":"PLoS Pathog"},{"issue":"12","key":"20_CR2","doi-asserted-by":"publisher","first-page":"e83989","DOI":"10.1371\/journal.pone.0083989","volume":"8","author":"CM Johnson","year":"2013","unstructured":"Johnson CM, Fisher DJ (2013) Site-specific, insertional inactivation of incA in Chlamydia trachomatis using a group II intron. PLoS One 8(12):e83989. https:\/\/doi.org\/10.1371\/journal.pone.0083989","journal-title":"PLoS One"},{"key":"20_CR3","doi-asserted-by":"publisher","unstructured":"Mueller KE, Wolf K, Fields KA (2016) Gene deletion by fluorescence-reported allelic exchange mutagenesis in Chlamydia trachomatis. mBio 7(1). https:\/\/doi.org\/10.1128\/mBio.01817-15","DOI":"10.1128\/mBio.01817-15"},{"key":"20_CR4","doi-asserted-by":"publisher","first-page":"59","DOI":"10.3389\/fcimb.2018.00059","volume":"8","author":"SP Ouellette","year":"2018","unstructured":"Ouellette SP (2018) Feasibility of a conditional knockout system for Chlamydia based on CRISPR interference. Front Cell Infect Microbiol 8:59. https:\/\/doi.org\/10.3389\/fcimb.2018.00059","journal-title":"Front Cell Infect Microbiol"},{"issue":"7","key":"20_CR5","doi-asserted-by":"publisher","first-page":"e0010821","DOI":"10.1128\/iai.00108-21","volume":"89","author":"SP Ouellette","year":"2021","unstructured":"Ouellette SP, Blay EA, Hatch ND, Fisher-Marvin LA (2021) CRISPR interference to inducibly repress gene expression in Chlamydia trachomatis. Infect Immun 89(7):e0010821. https:\/\/doi.org\/10.1128\/iai.00108-21","journal-title":"Infect Immun"},{"key":"20_CR6","doi-asserted-by":"publisher","unstructured":"LaBrie SD, Dimond ZE, Harrison KS, Baid S, Wickstrum J, Suchland RJ, Hefty PS (2019) Transposon mutagenesis in Chlamydia trachomatis identifies CT339 as a ComEC homolog important for DNA uptake and lateral gene transfer. mBio 10(4). https:\/\/doi.org\/10.1128\/mBio.01343-19","DOI":"10.1128\/mBio.01343-19"},{"key":"20_CR7","doi-asserted-by":"publisher","unstructured":"Wang Y, LaBrie SD, Carrell SJ, Suchland RJ, Dimond ZE, Kwong F, Rockey DD, Hefty PS, Hybiske K (2019) Development of transposon mutagenesis for Chlamydia muridarum. J Bacteriol 201(23). https:\/\/doi.org\/10.1128\/JB.00366-19","DOI":"10.1128\/JB.00366-19"},{"issue":"11","key":"20_CR8","doi-asserted-by":"publisher","first-page":"e0311630","DOI":"10.1371\/journal.pone.0311630","volume":"19","author":"Y Wang","year":"2024","unstructured":"Wang Y, Suchland R, Hua A, Carrell S, Rockey D, Hybiske K (2024) Development of a lambda Red based system for gene deletion in Chlamydia. PLoS One 19(11):e0311630. https:\/\/doi.org\/10.1371\/journal.pone.0311630","journal-title":"PLoS One"},{"issue":"9","key":"20_CR9","doi-asserted-by":"publisher","first-page":"e1010818","DOI":"10.1371\/journal.ppat.1010818","volume":"18","author":"ME Cortina","year":"2022","unstructured":"Cortina ME, Bishop RC, DeVasure BA, Coppens I, Derr\u00e9 I (2022) The inclusion membrane protein IncS is critical for initiation of the Chlamydia intracellular developmental cycle. PLoS Pathog 18(9):e1010818. https:\/\/doi.org\/10.1371\/journal.ppat.1010818","journal-title":"PLoS Pathog"},{"issue":"1","key":"20_CR10","doi-asserted-by":"publisher","first-page":"e0203623","DOI":"10.1128\/mbio.02036-23","volume":"15","author":"B Lu","year":"2024","unstructured":"Lu B, Wang Y, Wurihan W, Cheng A, Yeung S, Fondell JD, Lai Z, Wan D, Wu X, Li WV, Fan H (2024) Requirement of GrgA for Chlamydia infectious progeny production, optimal growth, and efficient plasmid maintenance. mBio 15(1):e0203623. https:\/\/doi.org\/10.1128\/mbio.02036-23","journal-title":"mBio"},{"issue":"2","key":"20_CR11","doi-asserted-by":"publisher","first-page":"e0254524","DOI":"10.1128\/mbio.02545-24","volume":"16","author":"J Ehses","year":"2025","unstructured":"Ehses J, Wang K, Densi A, Ramirez C, Tan M, Sutterlin C (2025) Development of an sRNA-mediated conditional knockdown system for Chlamydia trachomatis. mBio 16(2):e0254524. https:\/\/doi.org\/10.1128\/mbio.02545-24","journal-title":"mBio"},{"key":"20_CR12","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1007\/978-1-4939-6472-7_11","volume":"1498","author":"CE Key","year":"2017","unstructured":"Key CE, Fisher DJ (2017) Use of group II Intron technology for targeted mutagenesis in Chlamydia trachomatis. Methods Mol Biol 1498:163\u2013177. https:\/\/doi.org\/10.1007\/978-1-4939-6472-7_11","journal-title":"Methods Mol Biol"},{"key":"20_CR13","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1007\/978-1-4939-9694-0_12","volume":"2042","author":"MM Weber","year":"2019","unstructured":"Weber MM, Faris R (2019) Mutagenesis of Chlamydia trachomatis using TargeTron. Methods Mol Biol 2042:165\u2013184. https:\/\/doi.org\/10.1007\/978-1-4939-9694-0_12","journal-title":"Methods Mol Biol"},{"issue":"7","key":"20_CR14","doi-asserted-by":"publisher","first-page":"1406","DOI":"10.1016\/j.celrep.2017.04.058","volume":"19","author":"MM Weber","year":"2017","unstructured":"Weber MM, Lam JL, Dooley CA, Noriea NF, Hansen BT, Hoyt FH, Carmody AB, Sturdevant GL, Hackstadt T (2017) Absence of specific Chlamydia trachomatis inclusion membrane proteins triggers premature inclusion membrane lysis and host cell death. Cell Rep 19(7):1406\u20131417. https:\/\/doi.org\/10.1016\/j.celrep.2017.04.058","journal-title":"Cell Rep"},{"key":"20_CR15","doi-asserted-by":"publisher","first-page":"415","DOI":"10.3389\/fcimb.2018.00415","volume":"8","author":"JH Shaw","year":"2018","unstructured":"Shaw JH, Key CE, Snider TA, Sah P, Shaw EI, Fisher DJ, Lutter EI (2018) Genetic Inactivation of Chlamydia trachomatis inclusion membrane protein CT228 alters MYPT1 recruitment, extrusion production, and longevity of infection. Front Cell Infect Microbiol 8:415. https:\/\/doi.org\/10.3389\/fcimb.2018.00415","journal-title":"Front Cell Infect Microbiol"},{"key":"20_CR16","doi-asserted-by":"publisher","first-page":"145","DOI":"10.3389\/fcimb.2018.00145","volume":"8","author":"MM Cosse","year":"2018","unstructured":"Cosse MM, Barta ML, Fisher DJ, Oesterlin LK, Niragire B, Perrinet S, Millot GA, Hefty PS, Subtil A (2018) The loss of expression of a single type 3 effector (CT622) strongly reduces Chlamydia trachomatis infectivity and growth. Front Cell Infect Microbiol 8:145. https:\/\/doi.org\/10.3389\/fcimb.2018.00145","journal-title":"Front Cell Infect Microbiol"},{"key":"20_CR17","doi-asserted-by":"publisher","unstructured":"Huang Y, Wang Y, Pan M, Wan D, Wang L, Fondell JD, Wu X, Zhong G, Fan H (2025) A lineage-specific heat-induced feedback loop controls HrcA to promote chlamydial fitness under stress. bioRxiv. https:\/\/doi.org\/10.1101\/2025.05.30.657042","DOI":"10.1101\/2025.05.30.657042"},{"issue":"6","key":"20_CR18","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00358-26","volume":"11","author":"Y Wang","year":"2026","unstructured":"Wang Y, Pan M, Coker TV, Wang J, Wang L, Jung Y, Zhong G, Fan H (2026) Chlamydial histone homologs control developmental fitness in the next infection cycle. mSphere 11(6), e0035826. https:\/\/doi.org\/10.1128\/msphere.00358-26","journal-title":"mSphere"},{"issue":"3","key":"20_CR19","doi-asserted-by":"publisher","first-page":"1161","DOI":"10.1128\/iai.31.3.1161-1176.1981","volume":"31","author":"HD Caldwell","year":"1981","unstructured":"Caldwell HD, Kromhout J, Schachter J (1981) Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis. Infect Immun 31(3):1161\u20131176","journal-title":"Infect Immun"},{"issue":"8","key":"20_CR20","doi-asserted-by":"publisher","first-page":"e00132","DOI":"10.1128\/spectrum.00132-24","volume":"12","author":"Y Wang","year":"2024","unstructured":"Wang Y, Fondell Joseph D, Zhong G, Fan H (2024) rRNA expression kinetics during the chlamydial developmental cycle. Microbiology Spectrum 12(8):e00132\u2013e00124. https:\/\/doi.org\/10.1128\/spectrum.00132-24","journal-title":"Microbiology Spectrum"},{"issue":"4","key":"20_CR21","doi-asserted-by":"publisher","first-page":"e0073821","DOI":"10.1128\/mSystems.00738-21","volume":"6","author":"W Wurihan","year":"2021","unstructured":"Wurihan W, Zou Y, Weber AM, Weldon K, Huang Y, Bao X, Zhu C, Wu X, Wang Y, Lai Z, Fan H (2021) Identification of a GrgA-Euo-HrcA transcriptional regulatory network in Chlamydia. mSystems 6(4):e0073821. https:\/\/doi.org\/10.1128\/mSystems.00738-21","journal-title":"mSystems"}],"container-title":["Methods in Molecular Biology","Chlamydia trachomatis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5531-3_20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T06:02:46Z","timestamp":1790056966000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5531-3_20"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,23]]},"ISBN":["9781071655306","9781071655313"],"references-count":21,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5531-3_20","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,23]]},"assertion":[{"value":"23 September 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}},{"value":"The authors have no conflicts of interest to declare that are relevant to the content of this chapter.","order":1,"name":"Ethics","label":"Conflict of Interest","group":{"name":"EthicsHeading","label":"Ethics"}}]}}