{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T07:01:00Z","timestamp":1790060460421,"version":"4.0.1"},"publisher-location":"New York, NY","reference-count":20,"publisher":"Springer US","isbn-type":[{"value":"9781071655306","type":"print"},{"value":"9781071655313","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2027]]},"DOI":"10.1007\/978-1-0716-5531-3_23","type":"book-chapter","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T05:33:44Z","timestamp":1790055224000},"page":"331-347","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Experimental Evolution of Drug Resistance for Antibiotic Target Discovery in Chlamydia trachomatis"],"prefix":"10.1007","author":[{"given":"Aakriti","family":"Singh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara S.","family":"Sixt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,23]]},"reference":[{"issue":"6","key":"23_CR1","doi-asserted-by":"publisher","first-page":"735","DOI":"10.1016\/j.bcp.2005.12.006","volume":"71","author":"J Alvesalo","year":"2006","unstructured":"Alvesalo J, Vuorela H, Tammela P et al (2006) Inhibitory effect of dietary phenolic compounds on Chlamydia pneumoniae in cell cultures. Biochem Pharmacol 71(6):735\u2013741. https:\/\/doi.org\/10.1016\/j.bcp.2005.12.006","journal-title":"Biochem Pharmacol"},{"issue":"8","key":"23_CR2","doi-asserted-by":"publisher","first-page":"4296","DOI":"10.1128\/AAC.00356-12","volume":"56","author":"KM Sandoz","year":"2012","unstructured":"Sandoz KM, Eriksen SG, Jeffrey BM et al (2012) Resistance to a novel antichlamydial compound is mediated through mutations in Chlamydia trachomatis secY. Antimicrob Agents Chemother 56(8):4296\u20134302. https:\/\/doi.org\/10.1128\/AAC.00356-12","journal-title":"Antimicrob Agents Chemother"},{"issue":"5","key":"23_CR3","doi-asserted-by":"publisher","first-page":"2968","DOI":"10.1128\/AAC.02015-13","volume":"58","author":"S Marwaha","year":"2014","unstructured":"Marwaha S, Uvell H, Salin O et al (2014) N-acylated derivatives of sulfamethoxazole and sulfafurazole inhibit intracellular growth of Chlamydia trachomatis. Antimicrob Agents Chemother 58(5):2968\u20132971. https:\/\/doi.org\/10.1128\/AAC.02015-13","journal-title":"Antimicrob Agents Chemother"},{"key":"23_CR4","doi-asserted-by":"publisher","first-page":"3151","DOI":"10.3389\/fmicb.2018.03151","volume":"9","author":"SA Mojica","year":"2018","unstructured":"Mojica SA, Eriksson AU, Davis RA et al (2018) Red fluorescent Chlamydia trachomatis applied to live cell imaging and screening for antibacterial agents. Front Microbiol 9:3151. https:\/\/doi.org\/10.3389\/fmicb.2018.03151","journal-title":"Front Microbiol"},{"issue":"7","key":"23_CR5","doi-asserted-by":"publisher","first-page":"101643","DOI":"10.1016\/j.xcrm.2024.101643","volume":"5","author":"K Knapp","year":"2024","unstructured":"Knapp K, Klasinc R, Koren A et al (2024) Combination of compound screening with an animal model identifies pentamidine to prevent Chlamydia trachomatis infection. Cell Rep Med 5(7):101643. https:\/\/doi.org\/10.1016\/j.xcrm.2024.101643","journal-title":"Cell Rep Med"},{"issue":"4","key":"23_CR6","doi-asserted-by":"publisher","first-page":"e3003123","DOI":"10.1371\/journal.pbio.3003123","volume":"23","author":"M \u00d6lander","year":"2025","unstructured":"\u00d6lander M, Rea Vazquez D, Meier K et al (2025) A multi-strategy antimicrobial discovery approach reveals new ways to treat Chlamydia. PLoS Biol 23(4):e3003123. https:\/\/doi.org\/10.1371\/journal.pbio.3003123","journal-title":"PLoS Biol"},{"issue":"1","key":"23_CR7","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-29789-3","volume":"15","author":"C Fleming","year":"2025","unstructured":"Fleming C, Nugent H, Quinteros SL et al (2025) A pilot study of re-purposing drugs to treat koalas with chlamydia. Sci Rep 15(1):45194. https:\/\/doi.org\/10.1038\/s41598-025-29789-3","journal-title":"Sci Rep"},{"issue":"12","key":"23_CR8","doi-asserted-by":"publisher","first-page":"e0143304","DOI":"10.1371\/journal.pone.0143304","volume":"10","author":"L Newman","year":"2015","unstructured":"Newman L, Rowley J, Vander Hoorn S et al (2015) Global estimates of the prevalence and incidence of four curable sexually transmitted infections in 2012 based on systematic review and global reporting. PLoS One 10(12):e0143304. https:\/\/doi.org\/10.1371\/journal.pone.0143304","journal-title":"PLoS One"},{"issue":"1","key":"23_CR9","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1038\/s41572-022-00359-5","volume":"8","author":"AW Solomon","year":"2022","unstructured":"Solomon AW, Burton MJ, Gower EW et al (2022) Trachoma. Nat Rev Dis Primers 8(1):32. https:\/\/doi.org\/10.1038\/s41572-022-00359-5","journal-title":"Nat Rev Dis Primers"},{"issue":"2","key":"23_CR10","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1517\/14656566.2015.999041","volume":"16","author":"SA Kohlhoff","year":"2015","unstructured":"Kohlhoff SA, Hammerschlag MR (2015) Treatment of chlamydial infections: 2014 update. Expert Opin Pharmacother 16(2):205\u2013212. https:\/\/doi.org\/10.1517\/14656566.2015.999041","journal-title":"Expert Opin Pharmacother"},{"key":"23_CR11","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1007\/s40588-016-0028-4","volume":"3","author":"N Borel","year":"2016","unstructured":"Borel N, Leonard C, Slade J, Schoborg RV (2016) Chlamydial antibiotic resistance and treatment failure in veterinary and human medicine. Curr Clin Microbiol Rep 3:10\u201318. https:\/\/doi.org\/10.1007\/s40588-016-0028-4","journal-title":"Curr Clin Microbiol Rep"},{"issue":"8","key":"23_CR12","doi-asserted-by":"publisher","first-page":"795","DOI":"10.1016\/S1473-3099(17)30364-X","volume":"17","author":"JS Jensen","year":"2017","unstructured":"Jensen JS (2017) Mycoplasma genitalium: yet another challenging STI. Lancet Infect Dis 17(8):795\u2013796. https:\/\/doi.org\/10.1016\/S1473-3099(17)30364-X","journal-title":"Lancet Infect Dis"},{"issue":"1","key":"23_CR13","doi-asserted-by":"publisher","first-page":"e14","DOI":"10.1016\/S1473-3099(18)30444-4","volume":"19","author":"KS O'Brien","year":"2019","unstructured":"O'Brien KS, Emerson P, Hooper PJ et al (2019) Antimicrobial resistance following mass azithromycin distribution for trachoma: a systematic review. Lancet Infect Dis 19(1):e14\u2013e25. https:\/\/doi.org\/10.1016\/S1473-3099(18)30444-4","journal-title":"Lancet Infect Dis"},{"issue":"7883","key":"23_CR14","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1038\/s41586-021-03986-2","volume":"599","author":"L Maier","year":"2021","unstructured":"Maier L, Goemans CV, Wirbel J et al (2021) Unravelling the collateral damage of antibiotics on gut bacteria. Nature 599(7883):120\u2013124. https:\/\/doi.org\/10.1038\/s41586-021-03986-2","journal-title":"Nature"},{"issue":"6","key":"23_CR15","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1038\/nrmicro.2016.30","volume":"14","author":"C Elwell","year":"2016","unstructured":"Elwell C, Mirrashidi K, Engel J (2016) Chlamydia cell biology and pathogenesis. Nat Rev Microbiol 14(6):385\u2013400. https:\/\/doi.org\/10.1038\/nrmicro.2016.30","journal-title":"Nat Rev Microbiol"},{"key":"23_CR16","doi-asserted-by":"publisher","first-page":"102330","DOI":"10.1016\/j.mib.2023.102330","volume":"74","author":"RJ Bastidas","year":"2023","unstructured":"Bastidas RJ, Valdivia RH (2023) The emerging complexity of Chlamydia trachomatis interactions with host cells as revealed by molecular genetic approaches. Curr Opin Microbiol 74:102330. https:\/\/doi.org\/10.1016\/j.mib.2023.102330","journal-title":"Curr Opin Microbiol"},{"issue":"5","key":"23_CR17","doi-asserted-by":"publisher","first-page":"668","DOI":"10.1039\/c5np00127g","volume":"33","author":"MA Farha","year":"2016","unstructured":"Farha MA, Brown ED (2016) Strategies for target identification of antimicrobial natural products. Nat Prod Rep 33(5):668\u2013680. https:\/\/doi.org\/10.1039\/c5np00127g","journal-title":"Nat Prod Rep"},{"key":"23_CR18","doi-asserted-by":"publisher","unstructured":"Mojica SA, Salin O, Bastidas RJ et al (2017) N-acylated derivatives of sulfamethoxazole block Chlamydia fatty acid synthesis and interact with FabF. Antimicrob Agents Chemother 61(10):e00716-17. https:\/\/doi.org\/10.1128\/AAC.00716-17","DOI":"10.1128\/AAC.00716-17"},{"issue":"2","key":"23_CR19","doi-asserted-by":"publisher","first-page":"e57090","DOI":"10.1371\/journal.pone.0057090","volume":"8","author":"H Agaisse","year":"2013","unstructured":"Agaisse H, Derre I (2013) A C. trachomatis cloning vector and the generation of C. trachomatis strains expressing fluorescent proteins under the control of a C. trachomatis promoter. PLoS One 8(2):e57090. https:\/\/doi.org\/10.1371\/journal.pone.0057090","journal-title":"PLoS One"},{"issue":"4","key":"23_CR20","doi-asserted-by":"publisher","first-page":"1263","DOI":"10.1073\/pnas.1117884109","volume":"109","author":"BD Nguyen","year":"2012","unstructured":"Nguyen BD, Valdivia RH (2012) Virulence determinants in the obligate intracellular pathogen Chlamydia trachomatis revealed by forward genetic approaches. Proc Natl Acad Sci U S A 109(4):1263\u20131268. https:\/\/doi.org\/10.1073\/pnas.1117884109","journal-title":"Proc Natl Acad Sci U S A"}],"container-title":["Methods in Molecular Biology","Chlamydia trachomatis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5531-3_23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T06:02:33Z","timestamp":1790056953000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5531-3_23"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,23]]},"ISBN":["9781071655306","9781071655313"],"references-count":20,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5531-3_23","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,23]]},"assertion":[{"value":"23 September 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}},{"value":"The authors declare that they have no conflicts of interest relevant to the content of this chapter.","order":1,"name":"Ethics","label":"Conflict of Interest","group":{"name":"EthicsHeading","label":"Ethics"}}]}}