{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T07:01:24Z","timestamp":1790060484548,"version":"4.0.1"},"publisher-location":"New York, NY","reference-count":19,"publisher":"Springer US","isbn-type":[{"value":"9781071655306","type":"print"},{"value":"9781071655313","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2027]]},"DOI":"10.1007\/978-1-0716-5531-3_7","type":"book-chapter","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T05:34:00Z","timestamp":1790055240000},"page":"95-107","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Labeling and Detection of Peptidoglycan in Chlamydia and Chlamydia-Related Organisms Utilizing D-Alanine Analogs"],"prefix":"10.1007","author":[{"given":"Josie","family":"Blair","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prakash","family":"Sah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Liechti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,23]]},"reference":[{"issue":"8","key":"7_CR1","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1038\/s41579-020-0366-3","volume":"18","author":"AJF Egan","year":"2020","unstructured":"Egan AJF, Errington J, Vollmer W (2020) Regulation of peptidoglycan synthesis and remodelling. Nat Rev Microbiol 18(8):446\u2013460. https:\/\/doi.org\/10.1038\/s41579-020-0366-3","journal-title":"Nat Rev Microbiol"},{"issue":"2","key":"7_CR2","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1111\/j.1574-6976.2007.00094.x","volume":"32","author":"W Vollmer","year":"2008","unstructured":"Vollmer W, Blanot D, de Pedro MA (2008) Peptidoglycan structure and architecture. FEMS Microbiol Rev 32(2):149\u2013167. https:\/\/doi.org\/10.1111\/j.1574-6976.2007.00094.x","journal-title":"FEMS Microbiol Rev"},{"key":"7_CR3","doi-asserted-by":"publisher","unstructured":"Garde S, Chodisetti PK, Reddy M (2021) Peptidoglycan: structure, synthesis, and regulation. EcoSal Plus 9(2):1\u201335. https:\/\/doi.org\/10.1128\/ecosalplus.ESP-0010-2020","DOI":"10.1128\/ecosalplus.ESP-0010-2020"},{"issue":"6","key":"7_CR4","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1038\/nrmicro.2016.30","volume":"14","author":"C Elwell","year":"2016","unstructured":"Elwell C, Mirrashidi K, Engel J (2016) Chlamydia cell biology and pathogenesis. Nat Rev Microbiol 14(6):385\u2013400. https:\/\/doi.org\/10.1038\/nrmicro.2016.30","journal-title":"Nat Rev Microbiol"},{"issue":"2","key":"7_CR5","first-page":"87","volume":"2","author":"JW Moulder","year":"1993","unstructured":"Moulder JW (1993) Why is Chlamydia sensitive to penicillin in the absence of peptidoglycan?. Infect Agents Dis 2(2):87\u201399","journal-title":"Infect Agents Dis"},{"issue":"Pt 10","key":"7_CR6","doi-asserted-by":"publisher","first-page":"2673","DOI":"10.1099\/00221287-144-10-2673","volume":"144","author":"I Chopra","year":"1998","unstructured":"Chopra I, Storey C, Falla TJ, Pearce JH (1998) Antibiotics, peptidoglycan synthesis and genomics: the chlamydial anomaly revisited. Microbiology (Reading) 144(Pt 10):2673\u20132678. https:\/\/doi.org\/10.1099\/00221287-144-10-2673","journal-title":"Microbiology (Reading)"},{"issue":"4","key":"7_CR7","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1016\/j.tim.2007.02.003","volume":"15","author":"MS Pavelka Jr","year":"2007","unstructured":"Pavelka Jr MS (2007) Another brick in the wall. Trends Microbiol 15(4):147\u2013149. https:\/\/doi.org\/10.1016\/j.tim.2007.02.003","journal-title":"Trends Microbiol"},{"issue":"2","key":"7_CR8","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1016\/j.tim.2005.12.004","volume":"14","author":"AJ McCoy","year":"2006","unstructured":"McCoy AJ, Maurelli AT (2006) Building the invisible wall: updating the Chlamydial peptidoglycan anomaly. Trends Microbiol 14(2):70\u201377. https:\/\/doi.org\/10.1016\/j.tim.2005.12.004","journal-title":"Trends Microbiol"},{"issue":"22","key":"7_CR9","doi-asserted-by":"publisher","first-page":"6507","DOI":"10.1128\/JB.185.22.6507-6512.2003","volume":"185","author":"L Hesse","year":"2003","unstructured":"Hesse L, Bostock J, Dementin S, Blanot D, Mengin-Lecreulx D, Chopra I (2003) Functional and biochemical analysis of Chlamydia trachomatis MurC, an enzyme displaying UDP-N-acetylmuramate:amino acid ligase activity. J Bacteriol 185(22):6507\u20136512. https:\/\/doi.org\/10.1128\/JB.185.22.6507-6512.2003","journal-title":"J Bacteriol"},{"issue":"24","key":"7_CR10","doi-asserted-by":"publisher","first-page":"7430","DOI":"10.1128\/JB.01029-09","volume":"191","author":"D Patin","year":"2009","unstructured":"Patin D, Bostock J, Blanot D, Mengin-Lecreulx D, Chopra I (2009) Functional and biochemical analysis of the Chlamydia trachomatis ligase MurE. J Bacteriol 191(24):7430\u20137435. https:\/\/doi.org\/10.1128\/JB.01029-09","journal-title":"J Bacteriol"},{"issue":"5389","key":"7_CR11","doi-asserted-by":"publisher","first-page":"754","DOI":"10.1126\/science.282.5389.754","volume":"282","author":"RS Stephens","year":"1998","unstructured":"Stephens RS, Kalman S, Lammel C, Fan J, Marathe R, Aravind L, Mitchell W, Olinger L, Tatusov RL, Zhao Q, Koonin EV, Davis RW (1998) Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis. Science 282(5389):754\u2013759. https:\/\/doi.org\/10.1126\/science.282.5389.754","journal-title":"Science"},{"issue":"12","key":"7_CR12","doi-asserted-by":"publisher","first-page":"2745","DOI":"10.1021\/acschembio.9b00664","volume":"14","author":"E Kuru","year":"2019","unstructured":"Kuru E, Radkov A, Meng X, Egan A, Alvarez L, Dowson A, Booher G, Breukink E, Roper DI, Cava F, Vollmer W, Brun Y, VanNieuwenhze MS (2019) Mechanisms of incorporation for D-amino acid probes that target peptidoglycan biosynthesis. ACS Chem Biol 14(12):2745\u20132756. https:\/\/doi.org\/10.1021\/acschembio.9b00664","journal-title":"ACS Chem Biol"},{"issue":"7489","key":"7_CR13","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1038\/nature12892","volume":"506","author":"GW Liechti","year":"2014","unstructured":"Liechti GW, Kuru E, Hall E, Kalinda A, Brun YV, VanNieuwenhze M, Maurelli AT (2014) A new metabolic cell-wall labelling method reveals peptidoglycan in Chlamydia trachomatis. Nature 506(7489):507\u2013510. https:\/\/doi.org\/10.1038\/nature12892","journal-title":"Nature"},{"issue":"5","key":"7_CR14","doi-asserted-by":"publisher","first-page":"e1005590","DOI":"10.1371\/journal.ppat.1005590","volume":"12","author":"G Liechti","year":"2016","unstructured":"Liechti G, Kuru E, Packiam M, Hsu YP, Tekkam S, Hall E, Rittichier JT, VanNieuwenhze M, Brun YV, Maurelli AT (2016) Pathogenic Chlamydia lack a classical sacculus but synthesize a narrow, mid-cell peptidoglycan ring, regulated by MreB, for cell division. PLoS Pathog 12(5):e1005590. https:\/\/doi.org\/10.1371\/journal.ppat.1005590","journal-title":"PLoS Pathog"},{"key":"7_CR15","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3856","volume":"4","author":"M Pilhofer","year":"2013","unstructured":"Pilhofer M, Aistleitner K, Biboy J, Gray J, Kuru E, Hall E, Brun YV, VanNieuwenhze MS, Vollmer W, Horn M, Jensen GJ (2013) Discovery of chlamydial peptidoglycan reveals bacteria with murein sacculi but without FtsZ. Nat Commun 4: 2856. https:\/\/doi.org\/10.1038\/ncomms3856","journal-title":"Nat Commun"},{"issue":"8","key":"7_CR16","doi-asserted-by":"publisher","first-page":"e0068520","DOI":"10.1128\/IAI.00685-20","volume":"89","author":"MR Brockett","year":"2021","unstructured":"Brockett MR, Liechti GW (2021) Persistence alters the interaction between Chlamydia trachomatis and its host cell. Infect Immun 89(8):e0068520. https:\/\/doi.org\/10.1128\/IAI.00685-20","journal-title":"Infect Immun"},{"key":"7_CR17","doi-asserted-by":"publisher","first-page":"733850","DOI":"10.3389\/fmicb.2021.733850","volume":"12","author":"GW Liechti","year":"2021","unstructured":"Liechti GW (2021) Localized peptidoglycan biosynthesis in Chlamydia trachomatis conforms to the polarized division and cell size reduction developmental models. Front Microbiol 12:733850. https:\/\/doi.org\/10.3389\/fmicb.2021.733850","journal-title":"Front Microbiol"},{"issue":"8","key":"7_CR18","doi-asserted-by":"publisher","first-page":"e1005822","DOI":"10.1371\/journal.ppat.1005822","volume":"12","author":"Y Abdelrahman","year":"2016","unstructured":"Abdelrahman Y, Ouellette SP, Belland RJ, Cox JV (2016) Polarized cell division of Chlamydia trachomatis. PLoS Pathog 12(8):e1005822. https:\/\/doi.org\/10.1371\/journal.ppat.1005822","journal-title":"PLoS Pathog"},{"issue":"1","key":"7_CR19","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-69397-x","volume":"10","author":"JV Cox","year":"2020","unstructured":"Cox JV, Abdelrahman YM, Ouellette SP (2020) Penicillin-binding proteins regulate multiple steps in the polarized cell division process of Chlamydia. Sci Rep 10(1):12588. https:\/\/doi.org\/10.1038\/s41598-020-69397-x","journal-title":"Sci Rep"}],"container-title":["Methods in Molecular Biology","Chlamydia trachomatis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5531-3_7","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T06:02:45Z","timestamp":1790056965000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5531-3_7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,23]]},"ISBN":["9781071655306","9781071655313"],"references-count":19,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5531-3_7","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,23]]},"assertion":[{"value":"23 September 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}