{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T14:20:13Z","timestamp":1789395613627,"version":"build-2803163510"},"publisher-location":"New York, NY","reference-count":33,"publisher":"Springer US","isbn-type":[{"value":"9781071655382","type":"print"},{"value":"9781071655399","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2027]]},"DOI":"10.1007\/978-1-0716-5539-9_15","type":"book-chapter","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T13:39:55Z","timestamp":1789393195000},"page":"265-272","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Cell Atlas of Adult Aedes aegypti Midgut Revealed by Single Nucleus\/Cell RNA Sequencing"],"prefix":"10.1007","author":[{"given":"Yingjun","family":"Cui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaotian","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tse-Yu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunlong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiming","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander WE","family":"Franz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"issue":"1","key":"15_CR1","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1038\/s41368-021-00146-0","volume":"13","author":"X Li","year":"2021","unstructured":"Li X, Wang CY (2021) From bulk, single-cell to spatial RNA sequencing. Int J Oral Sci 13(1):36. https:\/\/doi.org\/10.1038\/s41368-021-00146-0","journal-title":"Int J Oral Sci"},{"issue":"4","key":"15_CR2","doi-asserted-by":"publisher","first-page":"982","DOI":"10.1016\/j.cell.2018.05.057","volume":"174","author":"K Davie","year":"2018","unstructured":"Davie K, Janssens J, Koldere D, De Waegeneer M, Pech U, Kreft L, Aibar S, Makhzami S, Christiaens V, Gonz\u00e1lez-Blas CB, Poovathingal S, Hulselmans G, Spanier KI, Moerman T, Vanspauwen B, Geurs S, Voet T, Lammertyn J, Thienpont B, Liu S, Konstantinides N, Fiers M, Verstreken P, Aerts S (2018) A single-cell transcriptome atlas of the aging Drosophila brain. Cell 174(4):982. https:\/\/doi.org\/10.1016\/j.cell.2018.05.057","journal-title":"Cell"},{"issue":"3","key":"15_CR3","doi-asserted-by":"publisher","first-page":"1514","DOI":"10.1073\/pnas.1916820117","volume":"117","author":"RJ Hung","year":"2020","unstructured":"Hung RJ, Hu YH, Kirchner R, Liu YF, Xu CW, Comjean A, Tattikota SG, Li FG, Song W, Sui SH, Perrimon N (2020) A cell atlas of the adult midgut. Proc Natl Acad Sci USA 117(3):1514\u20131523. https:\/\/doi.org\/10.1073\/pnas.1916820117","journal-title":"Proc Natl Acad Sci USA"},{"issue":"6584","key":"15_CR4","doi-asserted-by":"publisher","first-page":"991","DOI":"10.1126\/science.abk2432","volume":"375","author":"HJ Li","year":"2022","unstructured":"Li HJ, Janssens J, De Waegeneer M, Kolluru SS, Davie K, Gardeux V, Saelens W, David F, Brbic M, Leskovec J, McLaughlin CN, Xie QJ, Jones RC, Brueckner K, Shim J, Tattikota SG, Schnorrer F, Rust K, Nystul TG, Carvalho-Santos Z, Ribeiro C, Pal S, Mahadevaraju S, Przytycka TM, Allen AM, Goodwin SF, Berry CW, Fuller MT, White-Cooper H, Matunis EL, DiNardo S, Galenza A, O\u2019Brien LE, Dow JAT, Jasper H, Oliver B, Perrimon N, Deplancke B, Quake SR, Luo LQ, Aerts S, Consortium F (2022) Fly cell atlas: a single-nucleus transcriptomic atlas of the adult fruit fly. Science 375(6584):991. https:\/\/doi.org\/10.1126\/science.abk2432","journal-title":"Science"},{"key":"15_CR5","doi-asserted-by":"publisher","unstructured":"Tattikota SG, Cho B, Liu Y, Hu Y, Barrera V, Steinbaugh MJ, Yoon SH, Comjean A, Li F, Dervis F, Hung RJ, Nam JW, Ho Sui S, Shim J, Perrimon N (2020) A single-cell survey of Drosophila blood. Elife 9:e54818. https:\/\/doi.org\/10.7554\/eLife.54818","DOI":"10.7554\/eLife.54818"},{"key":"15_CR6","doi-asserted-by":"publisher","unstructured":"Cui YJ, Franz AWE (2020) Heterogeneity of midgut cells and their differential responses to blood meal ingestion by the mosquito. Insect Biochem Mol 127:103496. https:\/\/doi.org\/10.1016\/j.ibmb.2020.103496","DOI":"10.1016\/j.ibmb.2020.103496"},{"issue":"32","key":"15_CR7","doi-asserted-by":"publisher","first-page":"E7568","DOI":"10.1073\/pnas.1803062115","volume":"115","author":"MS Severo","year":"2018","unstructured":"Severo MS, Landry JJM, Lindquist RL, Goosmann C, Brinkmann V, Collier P, Hauser AE, Benes V, Henriksson J, Teichmann SA, Levashina EA (2018) Unbiased classification of mosquito blood cells by single-cell genomics and high-content imaging. Proc Natl Acad Sci USA 115(32):E7568\u2013E7577. https:\/\/doi.org\/10.1073\/pnas.1803062115","journal-title":"Proc Natl Acad Sci USA"},{"issue":"6507","key":"15_CR8","doi-asserted-by":"publisher","first-page":"1128","DOI":"10.1126\/science.abc0322","volume":"369","author":"G Raddi","year":"2020","unstructured":"Raddi G, Barletta ABF, Efremova M, Ramirez JL, Cantera R, Teichmann SA, Barillas-Mury C, Billker O (2020) Mosquito cellular immunity at single-cell resolution. Science 369(6507):1128. https:\/\/doi.org\/10.1126\/science.abc0322","journal-title":"Science"},{"issue":"7","key":"15_CR9","doi-asserted-by":"publisher","first-page":"110353","DOI":"10.1016\/j.isci.2024.110353","volume":"27","author":"TY Chen","year":"2024","unstructured":"Chen TY, Raduwan H, Marin-Lopez A, Cui Y, Fikrig E (2024) Zika virus exists in enterocytes and enteroendocrine cells of the Aedes aegypti midgut. iScience 27(7):110353. https:\/\/doi.org\/10.1016\/j.isci.2024.110353","journal-title":"iScience"},{"key":"15_CR10","doi-asserted-by":"publisher","unstructured":"Goldman OV, DeFoe AE, Qi Y, Jiao Y, Weng SC, Houri-Zeevi L, Lakhiani P, Morita T, Razzauti J, Rosas-Villegas A, Tsitohay YN, Walker MM, Hopkins BR, Mosquito Cell Atlas C, Akbari OS, Duvall LB, White-Cooper H, Sorrells TR, Sharma R, Li H, Vosshall LB, Shai N (2025) Mosquito cell atlas: a single-nucleus transcriptomic atlas of the adult Aedes aegypti mosquito. BioRxiv. https:\/\/doi.org\/10.1101\/2025.02.25.639765","DOI":"10.1101\/2025.02.25.639765"},{"issue":"1","key":"15_CR11","doi-asserted-by":"publisher","first-page":"e1012855","DOI":"10.1371\/journal.ppat.1012855","volume":"21","author":"EA Fitzmeyer","year":"2025","unstructured":"Fitzmeyer EA, Dutt TS, Pinaud S, Graham B, Gallichotte EN, Hill JL, Campbell CL, Ogg H, Howick V, Lawniczak MKN, Nishimura EO, Merkling SH, Henao-Tamayo M, Ebel GD (2025) A single-cell atlas of the Culex tarsalis midgut during West Nile virus infection. Plos Pathog 21(1):e1012855. https:\/\/doi.org\/10.1371\/journal.ppat.1012855","journal-title":"Plos Pathog"},{"key":"15_CR12","doi-asserted-by":"publisher","unstructured":"Kwon H, Mohammed M, Franzen O, Ankarklev J, Smith RC (2021) Single-cell analysis of mosquito hemocytes identifies signatures of immune cell subtypes and cell differentiation. Elife 10. https:\/\/doi.org\/10.7554\/eLife.66192","DOI":"10.7554\/eLife.66192"},{"issue":"1","key":"15_CR13","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-022-08327-9","volume":"23","author":"Y Cui","year":"2022","unstructured":"Cui Y, Behura SK, Franz AWE (2022) Cellular diversity and gene expression profiles in the male and female brain of Aedes aegypti. BMC Genomics 23(1):119. https:\/\/doi.org\/10.1186\/s12864-022-08327-9","journal-title":"BMC Genomics"},{"issue":"1","key":"15_CR14","doi-asserted-by":"publisher","DOI":"10.1038\/s41597-024-03432-8","volume":"11","author":"S Wang","year":"2024","unstructured":"Wang S, Huang Y, Wang F, Han Q, Ren N, Wang X, Cui Y, Yuan Z, Xia H (2024) A cell atlas of the adult female Aedes aegypti midgut revealed by single-cell RNA sequencing. Sci Data 11(1):587. https:\/\/doi.org\/10.1038\/s41597-024-03432-8","journal-title":"Sci Data"},{"key":"15_CR15","doi-asserted-by":"publisher","unstructured":"Goldman OV, DeFoe AE, Qi Y, Jiao Y, Weng SC, Wick B, Houri-Zeevi L, Lakhiani P, Morita T, Razzauti J, Rosas-Villegas A, Tsitohay YN, Walker MM, Hopkins BR, Aedes aegypti Mosquito Cell Atlas Consortium, Haeussler M, Akbari OS, Duvall LB, White-Cooper H, Sorrells TR, Sharma R, Li H, Vosshall LB, Shai N (2025) A single-nucleus transcriptomic atlas of the adult Aedes aegypti mosquito. Cell 188:7267\u20137290. https:\/\/doi.org\/10.1016\/j.cell.2025.10.008","DOI":"10.1016\/j.cell.2025.10.008"},{"issue":"8","key":"15_CR16","doi-asserted-by":"publisher","first-page":"100924","DOI":"10.1016\/j.xgen.2025.100924","volume":"5","author":"T Vial","year":"2025","unstructured":"Vial T, Lopez-Maestre H, Couderc E, Pinaud S, Howick V, Akorli J, Lawniczak M, Marti G, Merkling SH (2025) Single-cell transcriptional landscapes of Aedes aegypti midgut and fat body after a bloodmeal. Cell Genom 5(8):100924. https:\/\/doi.org\/10.1016\/j.xgen.2025.100924","journal-title":"Cell Genom"},{"key":"15_CR17","doi-asserted-by":"publisher","unstructured":"Patir A, Raper A, Fleming R, Henderson BEP, Murphy L, Henderson NC, Clark EL, Freeman TC, Barnett MW (2023) Cellular heterogeneity of the developing worker honey bee (Apis mellifera) pupa: a single cell transcriptomics analysis. G3-Genes Genom Genet 13(10):jkad178. https:\/\/doi.org\/10.1093\/g3journal\/jkad178","DOI":"10.1093\/g3journal\/jkad178"},{"key":"15_CR18","doi-asserted-by":"publisher","unstructured":"Zhang WX, Wang LL, Zhao YJ, Wang YF, Chen CY, Hu Y, Zhu YX, Sun H, Cheng Y, Sun QM, Zhang J, Chen DH (2022) Single-cell transcriptomic analysis of honeybee brains identifies vitellogenin as caste differentiation-related factor. Iscience 25(7):104643. https:\/\/doi.org\/10.1016\/j.isci.2022.104643","DOI":"10.1016\/j.isci.2022.104643"},{"issue":"1","key":"15_CR19","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-58614-8","volume":"16","author":"X Mu","year":"2025","unstructured":"Mu X, Zhang Z, Liu Q, Ma J, Qin Y, Lang H, Zhang Y, Zhang N, Guo Q, Zhang P, Li D, Zhang R, Ji Q, Jiang A, Wang Y, Pan S, Liu X, Liu X, Sun J, Liu Y, Chen H, Zheng L, Meng L, Lu H, Zhang H, Zhai Y, Li Q, Liu J, Yang H, Wang J, Hu X, Xu X, Liu S, Zheng H (2025) Single-nucleus and spatial transcriptomics identify brain landscape of gene regulatory networks associated with behavioral maturation in honeybees. Nat Commun 16(1):3343. https:\/\/doi.org\/10.1038\/s41467-025-58614-8","journal-title":"Nat Commun"},{"key":"15_CR20","doi-asserted-by":"publisher","unstructured":"Robben M, Ramesh B, Pau S, Meletis D, Luber J, Demuth J (2024) scRNA-seq reveals novel genetic pathways and sex chromosome regulation in Tribolium spermatogenesis. Genome Biol Evol 16(3):evae059. https:\/\/doi.org\/10.1093\/gbe\/evae059","DOI":"10.1093\/gbe\/evae059"},{"issue":"4","key":"15_CR21","doi-asserted-by":"publisher","first-page":"1041","DOI":"10.1111\/1744-7917.13294","volume":"31","author":"XJ Liu","year":"2024","unstructured":"Liu XJ, Zhang ZJ, Hu B, Chen K, Yu Y, Xiang H, Tan AJ (2024) Single-cell transcriptomes provide insights into expansion of glial cells in Bombyx mori. Insect Sci 31(4):1041\u20131054. https:\/\/doi.org\/10.1111\/1744-7917.13294","journal-title":"Insect Sci"},{"key":"15_CR22","doi-asserted-by":"publisher","unstructured":"Feng M, Xia JM, Fei SG, Peng RX, Wang X, Zhou YH, Wang PW, Swevers L, Sun JC (2021) Identification of silkworm hemocyte subsets and analysis of their response to baculovirus infection based on single-cell RNA sequencing. Front Immunol 12:645359. https:\/\/doi.org\/10.3389\/fimmu.2021.645359","DOI":"10.3389\/fimmu.2021.645359"},{"key":"15_CR23","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/978-1-0716-2756-3_1","volume":"2584","author":"K Danielski","year":"2023","unstructured":"Danielski K (2023) Guidance on processing the 10\u00d7 Genomics single cell gene expression assay. Methods Mol Biol 2584:1\u201328. https:\/\/doi.org\/10.1007\/978-1-0716-2756-3_1","journal-title":"Methods Mol Biol"},{"issue":"12","key":"15_CR24","doi-asserted-by":"publisher","first-page":"2742","DOI":"10.1038\/s41596-018-0073-y","volume":"13","author":"A Lafzi","year":"2018","unstructured":"Lafzi A, Moutinho C, Picelli S, Heyn H (2018) Tutorial: guidelines for the experimental design of single-cell RNA sequencing studies. Nat Protoc 13(12):2742\u20132757. https:\/\/doi.org\/10.1038\/s41596-018-0073-y","journal-title":"Nat Protoc"},{"issue":"6","key":"15_CR25","doi-asserted-by":"publisher","first-page":"737","DOI":"10.1038\/s41587-020-0465-8","volume":"38","author":"J Ding","year":"2020","unstructured":"Ding J, Adiconis X, Simmons SK, Kowalczyk MS, Hession CC, Marjanovic ND, Hughes TK, Wadsworth MH, Burks T, Nguyen LT, Kwon JYH, Barak B, Ge W, Kedaigle AJ, Carroll S, Li S, Hacohen N, Rozenblatt-Rosen O, Shalek AK, Villani AC, Regev A, Levin JZ (2020) Systematic comparison of single-cell and single-nucleus RNA-sequencing methods. Nat Biotechnol 38(6):737\u2013746. https:\/\/doi.org\/10.1038\/s41587-020-0465-8","journal-title":"Nat Biotechnol"},{"issue":"11","key":"15_CR26","doi-asserted-by":"publisher","first-page":"1333","DOI":"10.1038\/s41592-021-01282-5","volume":"18","author":"T Stuart","year":"2021","unstructured":"Stuart T, Srivastava A, Madad S, Lareau CA, Satija R (2021) Single-cell chromatin state analysis with Signac. Nat Methods 18(11):1333\u20131341. https:\/\/doi.org\/10.1038\/s41592-021-01282-5","journal-title":"Nat Methods"},{"issue":"5","key":"15_CR27","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1038\/nbt.3192","volume":"33","author":"R Satija","year":"2015","unstructured":"Satija R, Farrell JA, Gennert D, Schier AF, Regev A (2015) Spatial reconstruction of single-cell gene expression data. Nat Biotechnol 33(5):495\u2013502. https:\/\/doi.org\/10.1038\/nbt.3192","journal-title":"Nat Biotechnol"},{"issue":"2","key":"15_CR28","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1038\/s41587-023-01767-y","volume":"42","author":"Y Hao","year":"2024","unstructured":"Hao Y, Stuart T, Kowalski MH, Choudhary S, Hoffman P, Hartman A, Srivastava A, Molla G, Madad S, Fernandez-Granda C, Satija R (2024) Dictionary learning for integrative, multimodal and scalable single-cell analysis. Nat Biotechnol 42(2):293\u2013304. https:\/\/doi.org\/10.1038\/s41587-023-01767-y","journal-title":"Nat Biotechnol"},{"issue":"1","key":"15_CR29","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1874-1","volume":"20","author":"C Hafemeister","year":"2019","unstructured":"Hafemeister C, Satija R (2019) Normalization and variance stabilization of single-cell RNA-seq data using regularized negative binomial regression. Genome Biol 20(1):296. https:\/\/doi.org\/10.1186\/s13059-019-1874-1","journal-title":"Genome Biol"},{"issue":"5","key":"15_CR30","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1038\/nbt.4096","volume":"36","author":"A Butler","year":"2018","unstructured":"Butler A, Hoffman P, Smibert P, Papalexi E, Satija R (2018) Integrating single-cell transcriptomic data across different conditions, technologies, and species. Nat Biotechnol 36(5):411\u2013420. https:\/\/doi.org\/10.1038\/nbt.4096","journal-title":"Nat Biotechnol"},{"key":"15_CR31","doi-asserted-by":"publisher","unstructured":"Marianes A, Spradling AC (2013) Physiological and stem cell compartmentalization within the Drosophila midgut. Elife 2:e00886. https:\/\/doi.org\/10.7554\/eLife.00886","DOI":"10.7554\/eLife.00886"},{"issue":"2","key":"15_CR32","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1016\/j.celrep.2015.06.009","volume":"12","author":"D Dutta","year":"2015","unstructured":"Dutta D, Dobson AJ, Houtz PL, Glasser C, Revah J, Korzelius J, Patel PH, Edgar BA, Buchon N (2015) Regional cell-specific transcriptome mapping reveals regulatory complexity in the adult midgut. Cell Rep 12(2):346\u2013358. https:\/\/doi.org\/10.1016\/j.celrep.2015.06.009","journal-title":"Cell Rep"},{"issue":"3","key":"15_CR33","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1007\/s00441-019-03076-w","volume":"377","author":"S Caccia","year":"2019","unstructured":"Caccia S, Casartelli M, Tettamanti G (2019) The amazing complexity of insect midgut cells: types, peculiarities, and functions. Cell Tissue Res 377(3):505\u2013525. https:\/\/doi.org\/10.1007\/s00441-019-03076-w","journal-title":"Cell Tissue Res"}],"container-title":["Methods in Molecular Biology","Stem Cell Transcriptional Networks"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5539-9_15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T13:39:56Z","timestamp":1789393196000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5539-9_15"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,15]]},"ISBN":["9781071655382","9781071655399"],"references-count":33,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5539-9_15","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,15]]},"assertion":[{"value":"15 September 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}