{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T10:03:49Z","timestamp":1790071429557,"version":"4.0.1"},"publisher-location":"New York, NY","reference-count":11,"publisher":"Springer US","isbn-type":[{"value":"9781071655467","type":"print"},{"value":"9781071655474","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2027]]},"DOI":"10.1007\/978-1-0716-5547-4_15","type":"book-chapter","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T09:17:14Z","timestamp":1790068634000},"page":"299-309","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Oligo-Modified Poly(\u03b2-Amino Ester)-Mediated mRNA Delivery: A Protocol for Gene Editing Applications"],"prefix":"10.1007","author":[{"given":"Laia","family":"Mart\u00ed-Melero","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara P\u00e9rez","family":"Mart\u00edn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvador Borr\u00f3s","family":"G\u00f3mez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Guerra-Rebollo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,23]]},"reference":[{"key":"15_CR1","doi-asserted-by":"publisher","first-page":"568","DOI":"10.1016\/j.omtn.2023.04.019","volume":"32","author":"J Zhang","year":"2023","unstructured":"Zhang J, Cai X, Dou R et al (2023) Poly(\u03b2-amino ester)s-based nanovehicles: structural regulation and gene delivery. Mol Ther Nucleic Acids 32:568\u2013581","journal-title":"Mol Ther Nucleic Acids"},{"key":"15_CR2","doi-asserted-by":"publisher","first-page":"e1801359","DOI":"10.1002\/adhm.201801359","volume":"8","author":"Y Liu","year":"2019","unstructured":"Liu Y, Li Y, Keskin D et al (2019) Poly(\u03b2-amino esters): synthesis, formulations, and their biomedical applications. Adv Healthc Mater 8:e1801359","journal-title":"Adv Healthc Mater"},{"key":"15_CR3","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1158\/1535-7163.MCT-12-0956","volume":"12","author":"CD Kamat","year":"2013","unstructured":"Kamat CD, Shmueli RB, Connis N et al (2013) Poly(\u03b2-amino ester) nanoparticle delivery of TP53 has activity against small cell lung cancer in vitro and in vivo. Mol Cancer Ther 12:405\u2013415","journal-title":"Mol Cancer Ther"},{"key":"15_CR4","doi-asserted-by":"publisher","first-page":"1395","DOI":"10.1080\/17425247.2020.1796628","volume":"17","author":"J Karlsson","year":"2020","unstructured":"Karlsson J, Rhodes KR, Green JJ et al (2020) Poly(beta-amino ester)s as gene delivery vehicles: challenges and opportunities. Expert Opin Drug Deliv 17:1395\u20131410","journal-title":"Expert Opin Drug Deliv"},{"key":"15_CR5","doi-asserted-by":"publisher","first-page":"2147","DOI":"10.1016\/j.actbio.2013.12.054","volume":"10","author":"N Segovia","year":"2014","unstructured":"Segovia N, Dosta P, Cascante et al (2014) Oligopeptide-terminated poly(\u03b2-amino ester)s for highly efficient gene delivery and intracellular localization.. Acta Biomater 10:2147\u20132158","journal-title":"Acta Biomater"},{"key":"15_CR6","doi-asserted-by":"publisher","first-page":"e1800335","DOI":"10.1002\/adhm.201800335","volume":"7","author":"C Fornaguera","year":"2018","unstructured":"Fornaguera C, Guerra-Rebollo M, L\u00e1zaro M\u00c1 et al (2018) mRNA delivery system for targeting antigen-presenting cells in vivo. Adv Healthc Mater 7:e1800335","journal-title":"Adv Healthc Mater"},{"key":"15_CR7","doi-asserted-by":"publisher","first-page":"e1900849","DOI":"10.1002\/adhm.201900849","volume":"8","author":"C Fornaguera","year":"2019","unstructured":"Fornaguera C, Guerra-Rebollo M, L\u00e1zaro M\u00c1 et al (2019) In vivo retargeting of poly(beta amino ester) (OM-pBAE) nanoparticles is influenced by protein corona. Adv Healthc Mater 8:e1900849","journal-title":"Adv Healthc Mater"},{"key":"15_CR8","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1007\/5584_2019_467","volume":"1288","author":"C Fornaguera","year":"2020","unstructured":"Fornaguera C, Castells-Sala C, Borr\u00f3s S (2020) Unraveling polymeric nanoparticles cell uptake pathways: two decades working to understand nanoparticles journey to improve gene therapy. Adv Exp Med Biol 1288:117\u2013138","journal-title":"Adv Exp Med Biol"},{"key":"15_CR9","doi-asserted-by":"publisher","first-page":"1611","DOI":"10.1039\/D2NA00800A","volume":"5","author":"M Naval\u00f3n-L\u00f3pez","year":"2023","unstructured":"Naval\u00f3n-L\u00f3pez M, Dols-Perez A, Grijalvo S et al (2023) Unravelling the role of individual components in pBAE\/polynucleotide polyplexes in the synthesis of tailored carriers for specific applications: on the road to rational formulations. Nanoscale Adv 5:1611\u20131623","journal-title":"Nanoscale Adv"},{"key":"15_CR10","doi-asserted-by":"publisher","first-page":"654","DOI":"10.1016\/j.jconrel.2020.02.045","volume":"321","author":"X Gao","year":"2020","unstructured":"Gao X, Jin Z, Tan X et al (2020) Hyperbranched poly(\u03b2-amino ester) based polyplex nanoparticles for delivery of CRISPR\/Cas9 system and treatment of HPV infection-associated cervical cancer. J Control Release 321:654\u2013668","journal-title":"J Control Release"},{"key":"15_CR11","doi-asserted-by":"publisher","first-page":"1210","DOI":"10.1089\/hum.2021.152","volume":"32","author":"M Par\u00e9s","year":"2021","unstructured":"Par\u00e9s M, Fornaguera C, Vila-Juli\u00e0 F et al (2021) Preclinical assessment of a gene-editing approach in a mouse model of mitochondrial neurogastrointestinal encephalomyopathy. Hum Gene Ther 32:1210\u20131223","journal-title":"Hum Gene Ther"}],"container-title":["Methods in Molecular Biology","CRISPR Delivery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5547-4_15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T09:17:14Z","timestamp":1790068634000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5547-4_15"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,23]]},"ISBN":["9781071655467","9781071655474"],"references-count":11,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5547-4_15","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,23]]},"assertion":[{"value":"23 September 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}},{"value":"The authors have no conflicts of interest to declare that are relevant to the content of this chapter.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}}]}}