{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T09:44:53Z","timestamp":1792143893926,"version":"4.4.0"},"publisher-location":"New York, NY","reference-count":9,"publisher":"Springer US","isbn-type":[{"value":"9781071655863","type":"print"},{"value":"9781071655870","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2027]]},"DOI":"10.1007\/978-1-0716-5587-0_16","type":"book-chapter","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T08:54:48Z","timestamp":1792140888000},"page":"257-280","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Post-Translational Regulation Using Degrons in Ewing Sarcoma"],"prefix":"10.1007","author":[{"given":"Florian H.","family":"Geyer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randolph K.","family":"Larsen IV","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florencia","family":"Cidre-Aranaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David G.","family":"McFadden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,16]]},"reference":[{"key":"16_CR1","doi-asserted-by":"publisher","unstructured":"McGinnis JH et al (2024) Endogenous EWSR1-FLI1 degron alleles enable control of fusion oncoprotein expression in tumor cell lines and xenografts. bioRxiv https:\/\/doi.org\/10.1101\/2024.10.27.620498","DOI":"10.1101\/2024.10.27.620498"},{"issue":"6","key":"16_CR2","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1021\/cb800025k","volume":"3","author":"GV Los","year":"2008","unstructured":"Los GV et al. (2008) HaloTag: a novel protein labeling technology for cell imaging and protein analysis. ACS Chem Biol 3(6):373\u2013382","journal-title":"ACS Chem Biol"},{"issue":"2","key":"16_CR3","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1021\/acschembio.7b00549","volume":"13","author":"MK Schwinn","year":"2018","unstructured":"Schwinn MK et al. (2018) CRISPR-mediated tagging of endogenous proteins with a luminescent peptide. ACS Chem Biol 13(2):467\u2013474","journal-title":"ACS Chem Biol"},{"issue":"8","key":"16_CR4","doi-asserted-by":"publisher","first-page":"1831","DOI":"10.1021\/acschembio.5b00442","volume":"10","author":"DL Buckley","year":"2015","unstructured":"Buckley DL et al. (2015) HaloPROTACS: use of small molecule PROTACs to induce degradation of HaloTag fusion proteins. ACS Chem Biol 10(8):1831\u20131837","journal-title":"ACS Chem Biol"},{"issue":"5","key":"16_CR5","doi-asserted-by":"publisher","first-page":"882","DOI":"10.1021\/acschembio.8b01016","volume":"14","author":"H Tovell","year":"2019","unstructured":"Tovell H et al. (2019) Rapid and reversible knockdown of endogenously tagged endosomal proteins via an optimized HaloPROTAC degrader. ACS Chem Biol 14(5):882\u2013892","journal-title":"ACS Chem Biol"},{"issue":"8","key":"16_CR6","doi-asserted-by":"publisher","first-page":"e219","DOI":"10.1002\/cpz1.219","volume":"1","author":"Y Saito","year":"2021","unstructured":"Saito Y, Kanemaki MT (2021) Targeted protein depletion using the auxin-inducible degron 2 (AID2) system. Curr Protoc 1(8):e219","journal-title":"Curr Protoc"},{"issue":"1","key":"16_CR7","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-19532-z","volume":"11","author":"A Yesbolatova","year":"2020","unstructured":"Yesbolatova A et al. (2020) The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat Commun 11(1):5701","journal-title":"Nat Commun"},{"issue":"9","key":"16_CR8","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1038\/nchembio.1869","volume":"11","author":"HK Chung","year":"2015","unstructured":"Chung HK et al. (2015) Tunable and reversible drug control of protein production via a self-excising degron. Nat Chem Biol 11(9):713\u2013720","journal-title":"Nat Chem Biol"},{"issue":"11","key":"16_CR9","doi-asserted-by":"publisher","first-page":"2281","DOI":"10.1038\/nprot.2013.143","volume":"8","author":"FA Ran","year":"2013","unstructured":"Ran FA et al. (2013) Genome engineering using the CRISPR-Cas9 system. Nat Protoc 8(11):2281\u20132308. https:\/\/doi.org\/10.1038\/nprot.2013.143","journal-title":"Nat Protoc"}],"container-title":["Methods in Molecular Biology","Ewing Sarcoma"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5587-0_16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T08:54:49Z","timestamp":1792140889000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/chapter\/10.1007\/978-1-0716-5587-0_16"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,16]]},"ISBN":["9781071655863","9781071655870"],"references-count":9,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5587-0_16","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,16]]},"assertion":[{"value":"16 October 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}