{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T08:01:55Z","timestamp":1790755315440,"version":"4.1.0"},"publisher-location":"New York, NY","reference-count":33,"publisher":"Springer US","isbn-type":[{"value":"9781071656068","type":"print"},{"value":"9781071656075","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2027]]},"DOI":"10.1007\/978-1-0716-5607-5_10","type":"book-chapter","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T07:46:43Z","timestamp":1790754403000},"page":"127-144","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Flow Cytometric Immunophenotyping of Acute Lymphoblastic Leukemia"],"prefix":"10.1007","author":[{"given":"Dana","family":"Delgado","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jolene L.","family":"Cardinali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph A.","family":"DiGiuseppe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,1]]},"reference":[{"key":"10_CR1","unstructured":"Jaffe ES, Arber DA, Campo E, Quintanilla-Fend L, Orazi A, Rimsza LM et al (2025) Hematopathology, 3rd edn. Elsevier"},{"key":"10_CR2","unstructured":"WHO (2024) Haematolymphoid tumours. IARC Publications"},{"key":"10_CR3","unstructured":"Arber DA, Borowitz MJ, Cook JR, de Leval L, Goodlad JR, Hasserjian RP et al (2025) The international consensus classification of myeloid and lymphoid neoplasms. Wolters Kluwer"},{"key":"10_CR4","doi-asserted-by":"crossref","unstructured":"Miller KP, Kovach AE (2024) Immunophenotyping by flow cytometry of acute lymphoblastic leukemia\/lymphoma. In: Schmitz JL, Detrick B, O\u2019Gorman MRG (eds) Manual of molecular and clinical laboratory immunology, 9th edn, ASM Press","DOI":"10.1002\/9781683674023.ch106"},{"issue":"4","key":"10_CR5","doi-asserted-by":"publisher","first-page":"256","DOI":"10.1002\/cyto.b.21833","volume":"96","author":"JA DiGiuseppe","year":"2019","unstructured":"DiGiuseppe JA, Wood BL (2019) Applications of flow cytometric immunophenotyping in the diagnosis and posttreatment monitoring of B and T lymphoblastic leukemia\/lymphoma. Cytom B Clin Cytom 96(4):256\u2013265","journal-title":"Cytom B Clin Cytom"},{"issue":"3","key":"10_CR6","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1002\/cyto.b.22113","volume":"104","author":"X Chen","year":"2023","unstructured":"Chen X, Gao Q, Roshal M, Cherian S (2023) Flow cytometric assessment for minimal\/measurable residual disease in B lymphoblastic leukemia\/lymphoma in the era of immunotherapy. Cytom B Clin Cytom 104(3):205\u2013223","journal-title":"Cytom B Clin Cytom"},{"key":"10_CR7","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1002\/cyto.b.21239","volume":"90","author":"BL Wood","year":"2016","unstructured":"Wood BL (2016) Principles of minimal residual disease detection for hematopoietic neoplasms by flow cytometry. Cytom B Clin Cytom 90:47\u201353","journal-title":"Cytom B Clin Cytom"},{"issue":"6","key":"10_CR8","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1002\/cyto.b.21101","volume":"84","author":"G Gaipa","year":"2013","unstructured":"Gaipa G, Basso G, Biondi A, Campana D (2013) Detection of minimal residual disease in pediatric acute lymphoblastic leukemia. Cytom B Clin Cytom 84(6):359\u2013369","journal-title":"Cytom B Clin Cytom"},{"issue":"12","key":"10_CR9","doi-asserted-by":"publisher","first-page":"5477","DOI":"10.1182\/blood-2008-01-132837","volume":"111","author":"MJ Borowitz","year":"2008","unstructured":"Borowitz MJ, Devidas M, Hunger SP, Bowman WP, Carroll AJ, Carroll WL et al (2008) Clinical significance of minimal residual disease in childhood acute lymphoblastic leukemia and its relationship to other prognostic factors: a Children\u2019s Oncology Group study. Blood 111(12):5477\u20135485","journal-title":"Blood"},{"key":"10_CR10","doi-asserted-by":"crossref","unstructured":"Wood B (2004) Multicolor immunophenotyping: human immune system hematopoiesis. In: Darzynkiewicz Z, Roederer M, Tanke HJ (eds) Cytometry, 4th edn. New developments. Methods in cell biology, vol 75. Elsevier, San Diego, CA, pp 559\u2013576","DOI":"10.1016\/S0091-679X(04)75023-2"},{"issue":"5","key":"10_CR11","doi-asserted-by":"publisher","first-page":"680","DOI":"10.5858\/2006-130-680-CACFCI","volume":"130","author":"B Wood","year":"2006","unstructured":"Wood B (2006) 9-color and 10-color flow cytometry in the clinical laboratory. Arch Pathol Lab Med 130(5):680\u2013690","journal-title":"Arch Pathol Lab Med"},{"key":"10_CR12","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1002\/cyto.b.21528","volume":"94","author":"M Keeney","year":"2017","unstructured":"Keeney M, Wood BL, Hedley BD, DiGiuseppe JA, Stetler-Stevenson M, Paietta E et al (2017) A QA program for MRD testing demonstrates that systematic education can reduce discordance among experienced interpreters. Cytom B Clin Cytom 94:239\u2013249","journal-title":"Cytom B Clin Cytom"},{"key":"10_CR13","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1002\/cyto.b.21252","volume":"88B","author":"J DiGiuseppe","year":"2015","unstructured":"DiGiuseppe J, Tadmor MD, Pe\u2019er D (2015) Detection of minimal residual disease in B lymphoblastic leukemia using viSNE. Cytom B Clin Cytom 88B:294\u2013304","journal-title":"Cytom B Clin Cytom"},{"issue":"5","key":"10_CR14","doi-asserted-by":"publisher","first-page":"1872","DOI":"10.1111\/bjh.19390","volume":"204","author":"B Lebecque","year":"2024","unstructured":"Lebecque B, Besombes J, Dannus LT, De Antonio M, Cacheux V, Greze V et al (2024) Faster clinical decisions in B-cell acute lymphoblastic leukaemia: a single flow cytometric 12-colour tube improves diagnosis and minimal residual disease follow-up. Br J Haematol 204(5):1872\u20131881","journal-title":"Br J Haematol"},{"issue":"3","key":"10_CR15","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1002\/cyto.b.20408","volume":"74","author":"G Gaipa","year":"2008","unstructured":"Gaipa G, Basso G, Aliprandi S, Migliavacca M, Vallinoto C, Maglia O et al (2008) Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B-cell precursor acute lymphoblastic leukemia cells. Cytom B Clin Cytom 74(3):150\u2013155","journal-title":"Cytom B Clin Cytom"},{"key":"10_CR16","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1002\/cyto.b.20511","volume":"78","author":"M Roshal","year":"2010","unstructured":"Roshal M, Fromm JR, Winter S, Dunsmore K, Wood BL (2010) Immaturity associated antigens are lost during induction for T cell lymphoblastic leukemia: implications for minimal residual disease detection. Cytom B Clin Cytom 78:139\u2013146","journal-title":"Cytom B Clin Cytom"},{"issue":"8","key":"10_CR17","doi-asserted-by":"publisher","first-page":"2498","DOI":"10.1182\/blood.V98.8.2498","volume":"98","author":"RW McKenna","year":"2001","unstructured":"McKenna RW, Washington LT, Aquino DB, Picker LJ, Kroft SH (2001) Immunophenotypic analysis of hematogones (B-lymphocyte precursors) in 662 consecutive bone marrow specimens by 4-color flow cytometry. Blood 98(8):2498\u20132507","journal-title":"Blood"},{"issue":"4","key":"10_CR18","doi-asserted-by":"publisher","first-page":"320","DOI":"10.1056\/NEJMoa2312948","volume":"391","author":"MR Litzow","year":"2024","unstructured":"Litzow MR, Sun Z, Mattison RJ, Paietta EM, Roberts KG, Zhang Y et al (2024) Blinatumomab for MRD-negative acute lymphoblastic leukemia in adults. N Engl J Med 391(4):320\u2013333","journal-title":"N Engl J Med"},{"key":"10_CR19","unstructured":"Gupta S, Rau RE, Kairalla JA, Rabin KR, Wang C, Angiolillo AL et al (2024) Blinatumomab in standard-risk B-cell acute lymphoblastic leukemia in children. N Engl J Med"},{"key":"10_CR20","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1056\/NEJMoa1709919","volume":"378","author":"JH Park","year":"2018","unstructured":"Park JH, Riviere I, Gonen M, Wang X, Senechal B, Curran KJ et al (2018) Long-term follow-up of CD19 CAR therapy in acute lymphoblastic leukemia. N Engl J Med 378:449\u2013459","journal-title":"N Engl J Med"},{"issue":"1","key":"10_CR21","doi-asserted-by":"publisher","first-page":"e44","DOI":"10.1002\/cpcy.44","volume":"86","author":"S Cherian","year":"2018","unstructured":"Cherian S, Stetler-Stevenson M (2018) Flow cytometric monitoring for residual disease in B lymphoblastic leukemia post T cell engaging targeted therapies. Curr Protoc Cytom 86(1):e44","journal-title":"Curr Protoc Cytom"},{"issue":"26","key":"10_CR22","doi-asserted-by":"publisher","first-page":"4017","DOI":"10.1182\/blood-2014-12-580068","volume":"125","author":"SL Maude","year":"2015","unstructured":"Maude SL, Teachey DT, Porter DL, Grupp SA (2015) CD19-targeted chimeric antigen receptor T-cell therapy for acute lymphoblastic leukemia. Blood 125(26):4017\u20134023","journal-title":"Blood"},{"issue":"1","key":"10_CR23","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1002\/cyto.b.21482","volume":"94","author":"S Cherian","year":"2018","unstructured":"Cherian S, Miller V, McCullouch V, Dougherty K, Fromm JR, Wood BL (2018) A novel flow cytometric assay for detection of residual disease in patients with B-lymphoblastic leukemia\/lymphoma post anti-CD19 therapy. Cytom B Clin Cytom 94(1):112\u2013120","journal-title":"Cytom B Clin Cytom"},{"issue":"8","key":"10_CR24","doi-asserted-by":"publisher","first-page":"740","DOI":"10.1056\/NEJMoa1509277","volume":"375","author":"HM Kantarjian","year":"2016","unstructured":"Kantarjian HM, DeAngelo DJ, Stelljes M, Martinelli G, Liedtke M, Stock W et al (2016) Inotuzumab ozogamicin versus standard therapy for acute lymphoblastic leukemia. N Engl J Med 375(8):740\u2013753","journal-title":"N Engl J Med"},{"issue":"4","key":"10_CR25","doi-asserted-by":"publisher","first-page":"348","DOI":"10.1002\/ajh.10176","volume":"71","author":"MT Elghetany","year":"2002","unstructured":"Elghetany MT, Patel J (2002) Assessment of CD24 expression on bone marrow neutrophilic granulocytes: CD24 is a marker for the myelocytic stage of development. Am J Hematol 71(4):348\u2013349","journal-title":"Am J Hematol"},{"issue":"12","key":"10_CR26","doi-asserted-by":"publisher","DOI":"10.1038\/s41408-017-0023-x","volume":"7","author":"E Mejstrikova","year":"2017","unstructured":"Mejstrikova E, Hrusak O, Borowitz MJ, Whitlock JA, Brethon B, Trippett TM et al (2017) CD19-negative relapse of pediatric B-cell precursor acute lymphoblastic leukemia following blinatumomab treatment. Blood Cancer J 7(12):659","journal-title":"Blood Cancer J"},{"issue":"4","key":"10_CR27","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1002\/cyto.b.20469","volume":"76","author":"EZ Reineks","year":"2009","unstructured":"Reineks EZ, Osei ES, Rosenberg A, Auletta J, Meyerson HJ (2009) CD22 expression on blastic plasmacytoid dendritic cell neoplasms and reactivity of anti-CD22 antibodies to peripheral blood dendritic cells. Cytom B Clin Cytom 76(4):237\u2013248","journal-title":"Cytom B Clin Cytom"},{"issue":"3","key":"10_CR28","doi-asserted-by":"publisher","first-page":"602","DOI":"10.1111\/bjh.17382","volume":"193","author":"E Mikhailova","year":"2021","unstructured":"Mikhailova E, Semchenkova A, Illarionova O, Kashpor S, Brilliantova V, Zakharova E et al (2021) Relative expansion of CD19-negative very-early normal B-cell precursors in children with acute lymphoblastic leukaemia after CD19 targeting by blinatumomab and CAR-T cell therapy: implications for flow cytometric detection of minimal residual disease. Br J Haematol 193(3):602\u2013612","journal-title":"Br J Haematol"},{"key":"10_CR29","first-page":"388A","volume":"89","author":"H Shinoda-Matsuoka","year":"2009","unstructured":"Shinoda-Matsuoka H, DiGiuseppe JA (2009) Overexpression of CD49f in precursor B-cell acute lymphoblastic leukemia: efficiency of minimal residual disease (MRD) detection by flow cytometric immunophenotyping compared with commonly used markers. Mod Pathol 89:388A","journal-title":"Mod Pathol"},{"issue":"2","key":"10_CR30","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1002\/cyto.b.20440","volume":"76","author":"JA DiGiuseppe","year":"2009","unstructured":"DiGiuseppe JA, Fuller SG, Borowitz MJ (2009) Overexpression of CD49f in precursor B-cell acute lymphoblastic leukemia: potential usefulness in minimal residual disease detection. Cytom B Clin Cytom 76(2):150\u2013155","journal-title":"Cytom B Clin Cytom"},{"issue":"3","key":"10_CR31","doi-asserted-by":"publisher","first-page":"440","DOI":"10.1182\/blood.V77.3.440.440","volume":"77","author":"CH Pui","year":"1991","unstructured":"Pui CH, Frankel LS, Carroll AJ, Raimondi SC, Shuster JJ, Head DR et al (1991) Clinical characteristics and treatment outcome of childhood acute lymphoblastic leukemia with the t(4;11)(q21;q23): a collaborative study of 40 cases. Blood 77(3):440\u2013447","journal-title":"Blood"},{"issue":"8","key":"10_CR32","doi-asserted-by":"publisher","first-page":"1589","DOI":"10.1038\/sj.leu.2402989","volume":"17","author":"S Schwartz","year":"2003","unstructured":"Schwartz S, Rieder H, Schlager B, Burmeister T, Fischer L, Thiel E (2003) Expression of the human homologue of rat NG2 in adult acute lymphoblastic leukemia: close association with MLL rearrangement and a CD10(-)\/CD24(-)\/CD65s(+)\/CD15(+) B-cell phenotype. Leukemia 17(8):1589\u20131595","journal-title":"Leukemia"},{"issue":"6","key":"10_CR33","doi-asserted-by":"publisher","first-page":"964","DOI":"10.1002\/pbc.25410","volume":"62","author":"NN Shah","year":"2015","unstructured":"Shah NN, Stevenson MS, Yuan CM, Richards K, Delbrook C, Kreitman RJ et al (2015) Characterization of CD22 expression in acute lymphoblastic leukemia. Pediatr Blood Cancer 62(6):964\u2013969","journal-title":"Pediatr Blood Cancer"}],"container-title":["Methods in Molecular Biology","Immunophenotyping"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5607-5_10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T07:46:45Z","timestamp":1790754405000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-5607-5_10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,1]]},"ISBN":["9781071656068","9781071656075"],"references-count":33,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5607-5_10","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,1]]},"assertion":[{"value":"1 October 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}