{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T20:41:05Z","timestamp":1792701665711,"version":"4.5.0"},"publisher-location":"New York, NY","reference-count":23,"publisher":"Springer US","isbn-type":[{"value":"9781071656341","type":"print"},{"value":"9781071656358","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:00:00Z","timestamp":1792713600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:00:00Z","timestamp":1792713600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2027]]},"DOI":"10.1007\/978-1-0716-5635-8_3","type":"book-chapter","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T20:09:07Z","timestamp":1792699747000},"page":"23-34","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Artificial Feeding of Hard Ticks Using Silicone Membranes"],"prefix":"10.1007","author":[{"given":"Vincent C.","family":"Duru","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bahar E.","family":"Mustafa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul","family":"Ghafar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul","family":"Jabbar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ard M.","family":"Nijhof","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,23]]},"reference":[{"issue":"3","key":"3_CR1","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1007\/s10493-016-0084-8","volume":"70","author":"ML Levin","year":"2016","unstructured":"Levin ML, Schumacher LBM (2016) Manual for maintenance of multi-host ixodid ticks in the laboratory. Exp Appl Acarol 70(3):343\u2013367. https:\/\/doi.org\/10.1007\/s10493-016-0084-8","journal-title":"Exp Appl Acarol"},{"key":"3_CR2","unstructured":"Allan SA (2014) Tick rearing and in vitro feeding. In: Sonenshine D, Roe RM (eds) Biology of ticks. Oxford University Press, Oxford, pp 444\u2013473"},{"issue":"2\u20133","key":"3_CR3","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/s0304-4017(97)00027-7","volume":"71","author":"J Eckert","year":"1997","unstructured":"Eckert J (1997) Alternatives to animal experimentation in parasitology. Vet Parasitol 71(2\u20133):99\u2013120. https:\/\/doi.org\/10.1016\/s0304-4017(97)00027-7","journal-title":"Vet Parasitol"},{"issue":"9","key":"3_CR4","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1016\/j.pt.2007.07.010","volume":"23","author":"T Kr\u00f6ber","year":"2007","unstructured":"Kr\u00f6ber T, Guerin PM (2007) In vitro feeding assays for hard ticks. Trends Parasitol 23(9):445\u2013449. https:\/\/doi.org\/10.1016\/j.pt.2007.07.010","journal-title":"Trends Parasitol"},{"key":"3_CR5","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1002\/9783527802883.ch9","volume-title":"Ectoparasites: Drug discovery against moving targets","author":"AM Nijhof","year":"2018","unstructured":"Nijhof AM, Tyson KR (2018) In vitro feeding methods for hematophagous arthropods and their application in drug discovery. In: Meng CQ, Sluder AE (Eds.) Ectoparasites: Drug discovery against moving targets. Wiley-VCH Verlag GmbH & Co., Weinheim, 187\u2013204"},{"issue":"3","key":"3_CR6","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1017\/S0031182000000305","volume":"5","author":"E Hindle","year":"1912","unstructured":"Hindle E, Merriman G (1912) The sensory perceptions of Argas persicus (Oken). Parasitol 5(3):203\u2013216. https:\/\/doi.org\/10.1017\/S0031182000000305","journal-title":"Parasitol"},{"issue":"2","key":"3_CR7","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1007\/BF01193661","volume":"13","author":"EV Schwan","year":"1991","unstructured":"Schwan EV, Hutton D, Shields KJ, Townson S (1991) Artificial feeding and successful reproduction in Ornithodoros moubata moubata (Murray, 1877) (Acarina: Argasidae). Exp Appl Acarol 13(2):107\u2013115. https:\/\/doi.org\/10.1007\/BF01193661","journal-title":"Exp Appl Acarol"},{"issue":"1","key":"3_CR8","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1186\/s13071-019-3396-9","volume":"12","author":"JJ Fourie","year":"2019","unstructured":"Fourie JJ et al (2019) Transmission of Anaplasma phagocytophilum (Foggie, 1949) by Ixodes ricinus (Linnaeus, 1758) ticks feeding on dogs and artificial membranes. Parasit Vectors 12(1):136. https:\/\/doi.org\/10.1186\/s13071-019-3396-9","journal-title":"Parasit Vectors"},{"issue":"5","key":"3_CR9","doi-asserted-by":"publisher","first-page":"1057","DOI":"10.1016\/j.ttbdis.2018.03.033","volume":"9","author":"T Hart","year":"2018","unstructured":"Hart T, Yang X, Pal U, Lin YP (2018) Identification of Lyme borreliae proteins promoting vertebrate host blood-specific spirochete survival in Ixodes scapularis nymphs using artificial feeding chambers. Ticks Tick Borne Dis 9(5):1057\u20131063. https:\/\/doi.org\/10.1016\/j.ttbdis.2018.03.033","journal-title":"Ticks Tick Borne Dis"},{"issue":"1","key":"3_CR10","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1089\/153036601750137660","volume":"1","author":"KR Macaluso","year":"2001","unstructured":"Macaluso KR, Sonenshine DE, Ceraul SM, Azad AF (2001) Infection and transovarial transmission of rickettsiae in Dermacentor variabilis ticks acquired by artificial feeding. Vector Borne Zoonotic Dis 1(1):45\u201353. https:\/\/doi.org\/10.1089\/153036601750137660","journal-title":"Vector Borne Zoonotic Dis"},{"issue":"12","key":"3_CR11","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0169053","volume":"11","author":"S Tajeri","year":"2016","unstructured":"Tajeri S, Razmi G, Haghparast A (2016) Establishment of an artificial tick feeding system to study Theileria lestoquardi Infection. PLoS One 11(12):e0169053. https:\/\/doi.org\/10.1371\/journal.pone.0169053","journal-title":"PLoS One"},{"key":"3_CR12","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1111\/j.1749-6632.1996.tb53527.x","volume":"791","author":"AS Young","year":"1996","unstructured":"Young AS, Waladde SM, Morzaria SP (1996) Artificial feeding systems for ixodid ticks as a tool for study of pathogen transmission. Ann N Y Acad Sci 791:211\u2013218. https:\/\/doi.org\/10.1111\/j.1749-6632.1996.tb53527.x","journal-title":"Ann N Y Acad Sci"},{"issue":"7","key":"3_CR13","doi-asserted-by":"publisher","first-page":"1074","DOI":"10.3201\/eid1407.071110","volume":"14","author":"V Cotte","year":"2008","unstructured":"Cotte V et al (2008) Transmission of Bartonella henselae by Ixodes ricinus. Emerg Infect Dis 14(7):1074\u20131080. https:\/\/doi.org\/10.3201\/eid1407.071110","journal-title":"Emerg Infect Dis"},{"issue":"1","key":"3_CR14","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1038\/s41598-019-57390-y","volume":"10","author":"R Vimonish","year":"2020","unstructured":"Vimonish R et al (2020) Quantitative analysis of Anaplasma marginale acquisition and transmission by Dermacentor andersoni fed in vitro. Sci Rep 10(1):470. https:\/\/doi.org\/10.1038\/s41598-019-57390-y","journal-title":"Sci Rep"},{"issue":"1","key":"3_CR15","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1002\/ps.1293","volume":"63","author":"K Thomas","year":"2007","unstructured":"Thomas K, Guerin PM (2007) An in vitro feeding assay to test acaricides for control of hard ticks. Pest Manag Sci 63(1):17\u201322. https:\/\/doi.org\/10.1002\/ps.1293","journal-title":"Pest Manag Sci"},{"issue":"5","key":"3_CR16","doi-asserted-by":"publisher","first-page":"500","DOI":"10.1016\/j.ttbdis.2014.03.005","volume":"5","author":"AE Lew-Tabor","year":"2014","unstructured":"Lew-Tabor AE, Bruyeres AG, Zhang B, Rodriguez Valle M (2014) Rhipicephalus (Boophilus) microplus tick in vitro feeding methods for functional (dsRNA) and vaccine candidate (antibody) screening. Ticks Tick Borne Dis 5(5):500\u2013510. https:\/\/doi.org\/10.1016\/j.ttbdis.2014.03.005","journal-title":"Ticks Tick Borne Dis"},{"issue":"8","key":"3_CR17","doi-asserted-by":"publisher","first-page":"887","DOI":"10.1016\/0020-7519(95)00009-q","volume":"25","author":"F Kuhnert","year":"1995","unstructured":"Kuhnert F, Diehl PA, Guerin PM (1995) The life-cycle of the bont tick Amblyomma hebraeum in vitro. Int J Parasitol 25(8):887\u2013896. https:\/\/doi.org\/10.1016\/0020-7519(95)00009-q","journal-title":"Int J Parasitol"},{"issue":"1","key":"3_CR18","doi-asserted-by":"publisher","first-page":"444","DOI":"10.1038\/s41598-023-51052-w","volume":"14","author":"K Elati","year":"2024","unstructured":"Elati K et al (2024) In vitro feeding of all life stages of two-host Hyalomma excavatum and Hyalomma scupense and three-host Hyalomma dromedarii ticks. Sci Rep 14(1):444. https:\/\/doi.org\/10.1038\/s41598-023-51052-w","journal-title":"Sci Rep"},{"issue":"4","key":"3_CR19","doi-asserted-by":"publisher","first-page":"385","DOI":"10.3390\/vaccines9040385","volume":"9","author":"N Militzer","year":"2021","unstructured":"Militzer N et al (2021) Artificial feeding of all consecutive life stages of Ixodes ricinus. Vaccines (Basel) 9(4):385. https:\/\/doi.org\/10.3390\/vaccines9040385","journal-title":"Vaccines (Basel)"},{"issue":"12","key":"3_CR20","doi-asserted-by":"publisher","first-page":"1896","DOI":"10.1016\/j.cub.2018.04.038","volume":"28","author":"O Duron","year":"2018","unstructured":"Duron O et al (2018) Tick-bacteria mutualism depends on B vitamin synthesis pathways. Curr Biol 28(12):1896\u20131902e1895. https:\/\/doi.org\/10.1016\/j.cub.2018.04.038","journal-title":"Curr Biol"},{"issue":"1","key":"3_CR21","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1186\/s13071-017-2081-0","volume":"10","author":"JJ Trentelman","year":"2017","unstructured":"Trentelman JJ, Kleuskens JA, van de Crommert J, Schetters TP (2017) A new method for in vitro feeding of Rhipicephalus australis (formerly Rhipicephalus microplus) larvae: a valuable tool for tick vaccine development. Parasit Vectors 10(1):153. https:\/\/doi.org\/10.1186\/s13071-017-2081-0","journal-title":"Parasit Vectors"},{"issue":"1","key":"3_CR22","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1186\/s13071-017-2000-4","volume":"10","author":"C Krull","year":"2017","unstructured":"Krull C, Bohme B, Clausen PH, Nijhof AM (2017) Optimization of an artificial tick feeding assay for Dermacentor reticulatus. Parasit Vectors 10(1):60. https:\/\/doi.org\/10.1186\/s13071-017-2000-4","journal-title":"Parasit Vectors"},{"issue":"Pt 3","key":"3_CR23","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1017\/s0031182000079233","volume":"107","author":"WP Voigt","year":"1993","unstructured":"Voigt WP et al (1993) In vitro feeding of instars of the ixodid tick Amblyomma variegatum on skin membranes and its application to the transmission of Theileria mutans and Cowdria ruminatium. Parasitol 107(Pt 3):257\u2013263. https:\/\/doi.org\/10.1017\/s0031182000079233","journal-title":"Parasitol"}],"container-title":["Methods in Molecular Biology","Allergic Disease Models"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5635-8_3","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T20:09:08Z","timestamp":1792699748000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/chapter\/10.1007\/978-1-0716-5635-8_3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,23]]},"ISBN":["9781071656341","9781071656358"],"references-count":23,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5635-8_3","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,23]]},"assertion":[{"value":"23 October 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}},{"value":"The authors have no conflicts of interest to declare that are relevant to the content of this chapter.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}]}}