{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T18:38:42Z","timestamp":1792694322765,"version":"4.5.0"},"publisher-location":"New York, NY","reference-count":20,"publisher":"Springer US","isbn-type":[{"value":"9781071656624","type":"print"},{"value":"9781071656631","type":"electronic"}],"license":[{"start":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:00:00Z","timestamp":1792713600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:00:00Z","timestamp":1792713600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2027]]},"DOI":"10.1007\/978-1-0716-5663-1_27","type":"book-chapter","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T17:49:13Z","timestamp":1792691353000},"page":"397-401","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["ISSR Marker-Based Authentication of In Vitro-Derived Medicinal Plant Lines for Enhanced Phytochemical Production"],"prefix":"10.1007","author":[{"given":"W.M.A.C.","family":"Premasiri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.G.I.","family":"Madushani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.L.C.K.","family":"Fonseka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,23]]},"reference":[{"issue":"3","key":"27_CR1","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1038\/s41573-020-00114-z","volume":"20","author":"AG Atanasov","year":"2021","unstructured":"Atanasov AG, Zotchev SB, Dirsch VM, Supuran CT (2021) Natural products in drug discovery: advances and opportunities. Nat Rev Drug Discov 20(3):200\u2013216","journal-title":"Nat Rev Drug Discov"},{"issue":"6","key":"27_CR2","doi-asserted-by":"publisher","first-page":"1752","DOI":"10.3390\/molecules26061752","volume":"26","author":"RM Moraes","year":"2021","unstructured":"Moraes RM, Cerdeira AL, Louren\u00e7o MV (2021) Using micropropagation to develop medicinal plants into crops. Molecules 26(6):1752","journal-title":"Molecules"},{"key":"27_CR3","doi-asserted-by":"crossref","unstructured":"Anuruddi HIGK, Nakandalage N, Fonseka DK (2023) New insights for the production of medicinal plant materials: ex vitro and in vitro propagation. In: Biosynthesis of bioactive compounds in medicinal and aromatic plants: manipulation by conventional and biotechnological approaches. Springer, Cham, pp 181\u2013212","DOI":"10.1007\/978-3-031-35221-8_9"},{"issue":"1","key":"27_CR4","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-023-03531-0","volume":"13","author":"D Patel","year":"2023","unstructured":"Patel D, Sharma S, Reddy V (2023) ISSR marker selection for reproducible amplification in plant biotechnology studies. 3 Biotech 13(1):120","journal-title":"3 Biotech"},{"issue":"3","key":"27_CR5","first-page":"311","volume":"62","author":"N Rathore","year":"2021","unstructured":"Rathore N, Sharma P, Singh V (2021) ISSR-based genetic stability assessment of micropropagated plants. J Appl Genet 62(3):311\u2013322","journal-title":"J Appl Genet"},{"issue":"8","key":"27_CR6","first-page":"1057","volume":"39","author":"S Rani","year":"2020","unstructured":"Rani S, Verma R, Singh A (2020) Documentation of PCR-amplified ISSR products using gel imaging systems. Plant Cell Rep 39(8):1057\u20131065","journal-title":"Plant Cell Rep"},{"issue":"5","key":"27_CR7","first-page":"821","volume":"20","author":"S Ahmad","year":"2024","unstructured":"Ahmad S, Khan MR, Sharma P (2024) Evaluation of somaclonal variation in in vitro-derived plants using ISSR markers. Plant Biotechnol J 20(5):821\u2013835","journal-title":"Plant Biotechnol J"},{"issue":"4","key":"27_CR8","first-page":"455","volume":"15","author":"S Rani","year":"2021","unstructured":"Rani S, Singh A, Dhawan O (2021) Leaf sampling and storage for high-quality DNA extraction in medicinal plants. Plant Biotechnol Rep 15(4):455\u2013466","journal-title":"Plant Biotechnol Rep"},{"issue":"7","key":"27_CR9","first-page":"6547","volume":"50","author":"R Singh","year":"2023","unstructured":"Singh R, Verma R, Bairu MW (2023) Cluster analysis of ISSR data for genetic relationship assessment in in vitro-derived plants. Mol Biol Rep 50(7):6547\u20136559","journal-title":"Mol Biol Rep"},{"issue":"2","key":"27_CR10","first-page":"123","volume":"41","author":"A Sharma","year":"2023","unstructured":"Sharma A, Gupta R, Singh P (2023) CTAB-based DNA extraction for high secondary metabolite medicinal plants. Plant Mol Biol Report 41(2):123\u2013135","journal-title":"Plant Mol Biol Report"},{"issue":"2","key":"27_CR11","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1006\/geno.1994.1151","volume":"20","author":"E Zietkiewicz","year":"1994","unstructured":"Zietkiewicz E, Rafalski A, Labuda D (1994) Genome fingerprinting by SSR-anchored polymerase chain reaction amplification. Genomics 20(2):176\u2013183","journal-title":"Genomics"},{"issue":"1","key":"27_CR12","first-page":"15","volume":"42","author":"D Patel","year":"2024","unstructured":"Patel D, Rani S, Bairu MW (2024) Binary data analysis and statistical assessment of ISSR polymorphisms in plant populations. Plant Mol Biol Report 42(1):15\u201328","journal-title":"Plant Mol Biol Report"},{"issue":"5","key":"27_CR13","first-page":"821","volume":"20","author":"N Rathore","year":"2022","unstructured":"Rathore N, Ahmad S, Verma R (2022) Evaluation of somaclonal variation in in vitro-derived plants using ISSR markers. Plant Biotechnol J 20(5):821\u2013835","journal-title":"Plant Biotechnol J"},{"issue":"2","key":"27_CR14","first-page":"415","volume":"144","author":"R Verma","year":"2023","unstructured":"Verma R, Singh A, Bairu MW (2023) Reproducibility and reliability of ISSR markers in medicinal plant biotechnology. Plant Cell Tissue Organ Cult 144(2):415\u2013429","journal-title":"Plant Cell Tissue Organ Cult"},{"issue":"4","key":"27_CR15","first-page":"847","volume":"77","author":"MW Bairu","year":"2011","unstructured":"Bairu MW, Aremu AO, van Staden J (2011) The role of plant growth regulators in tissue culture of medicinal plants. S Afr J Botany 77(4):847\u2013857","journal-title":"S Afr J Botany"},{"key":"27_CR16","unstructured":"Sneath PHA, Sokal RR (1973) Numerical taxonomy: the principles and practice of numerical classification. W. H. Freeman and Company, San Francisco, CA"},{"key":"27_CR17","doi-asserted-by":"crossref","unstructured":"Biswas P, Nandy S, Dey A, Tikendra L, Nongdam P (2022) Molecular markers in assessing genetic clonal fidelity for in vitro propagated endangered medicinal plants. In: Molecular genetics and genomics tools in biodiversity conservation. Springer Nature, Singapore, pp 97\u2013149","DOI":"10.1007\/978-981-16-6005-4_6"},{"issue":"1","key":"27_CR18","first-page":"75","volume":"137","author":"S Ahmad","year":"2024","unstructured":"Ahmad S, Khan MR, Sharma P (2024) Genetic fidelity assessment of in vitro-propagated medicinal plants using ISSR markers. Plant Cell Tissue Organ Cult 137(1):75\u201388","journal-title":"Plant Cell Tissue Organ Cult"},{"issue":"5","key":"27_CR19","doi-asserted-by":"crossref","first-page":"4567","DOI":"10.1007\/s11033-022-07186-7","volume":"49","author":"S Kumar","year":"2022","unstructured":"Kumar S, Pandey V, Singh P (2022) Spectrophotometric assessment of DNA quality and purity in plant molecular studies. Mol Biol Rep 49(5):4567\u20134575","journal-title":"Mol Biol Rep"},{"key":"27_CR20","doi-asserted-by":"crossref","unstructured":"Gupta S, Singh A, Yadav K, Pandey N, Kumar S (2022) Micropropagation for multiplication of disease-free and genetically uniform sugarcane plantlets. In: Advances in plant tissue culture. Academic Press, pp 31\u201349","DOI":"10.1016\/B978-0-323-90795-8.00015-1"}],"container-title":["Methods in Molecular Biology","Molecular Markers in Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-5663-1_27","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T17:49:14Z","timestamp":1792691354000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/chapter\/10.1007\/978-1-0716-5663-1_27"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,23]]},"ISBN":["9781071656624","9781071656631"],"references-count":20,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-5663-1_27","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,23]]},"assertion":[{"value":"23 October 2026","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}