{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T23:40:04Z","timestamp":1793490004737,"version":"4.5.0"},"publisher-location":"New York, NY","reference-count":30,"publisher":"Springer New York","isbn-type":[{"value":"9781493920730","type":"print"},{"value":"9781493920747","type":"electronic"}],"license":[{"start":{"date-parts":[[2014,11,3]],"date-time":"2014-11-03T00:00:00Z","timestamp":1414972800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2014,11,3]],"date-time":"2014-11-03T00:00:00Z","timestamp":1414972800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015]]},"DOI":"10.1007\/978-1-4939-2074-7_12","type":"book-chapter","created":{"date-parts":[[2014,11,21]],"date-time":"2014-11-21T11:54:19Z","timestamp":1416570859000},"page":"175-188","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Primary Hepatocytes in Sandwich Culture"],"prefix":"10.1007","author":[{"given":"Janneke","family":"Keemink","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlies","family":"Oorts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pieter","family":"Annaert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2014,11,3]]},"reference":[{"key":"12_CR1","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1096\/fasebj.3.2.2914628","volume":"3","author":"JC Dunn","year":"1989","unstructured":"Dunn JC, Yarmush ML, Koebe HD et al (1989) Hepatocyte function and extracellular matrix geometry: long-term culture in a sandwich configuration. FASEB J 3:174\u2013177","journal-title":"FASEB J"},{"key":"12_CR2","doi-asserted-by":"publisher","first-page":"1658","DOI":"10.1124\/dmd.105.009118","volume":"34","author":"Y Bi","year":"2006","unstructured":"Bi Y, Kazolias D, Duignan DB (2006) Use of cryopreserved human hepatocytes in sandwich culture to measure hepatobiliary transport. Drug Metab Dispos 34:1658\u20131665","journal-title":"Drug Metab Dispos"},{"key":"12_CR3","doi-asserted-by":"crossref","first-page":"2886","DOI":"10.1002\/jps.22018","volume":"99","author":"Z Ye","year":"2010","unstructured":"Ye Z, Van Pelt J, Camus S et al (2010) Species-specific interaction of HIV protease inhibitors with accumulation of cholyl-glycylamido-fluorescein (CGamF) in sandwich-cultured hepatocytes. J Pharm Sci 99:2886\u20132898","journal-title":"J Pharm Sci"},{"key":"12_CR4","doi-asserted-by":"publisher","first-page":"884","DOI":"10.1124\/dmd.32.8.884","volume":"32","author":"N Treijtel","year":"2004","unstructured":"Treijtel N, Barendregt A, Freidig AP et al (2004) Modeling the in vitro intrinsic clearance of the slowly metabolized compound tolbutamide determined in sandwich-cultured rat hepatocytes. Drug Metab Dispos 32: 884\u2013891","journal-title":"Drug Metab Dispos"},{"key":"12_CR5","doi-asserted-by":"publisher","first-page":"1503","DOI":"10.1124\/dmd.111.038968","volume":"39","author":"N Kotani","year":"2011","unstructured":"Kotani N, Maeda K, Watanabe T et al (2011) Culture period-dependent changes in the uptake of transporter substrates in sandwich-cultured rat and human hepatocytes. Drug Metab Dispos 39:1503\u20131510","journal-title":"Drug Metab Dispos"},{"key":"12_CR6","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1016\/j.ejps.2011.09.001","volume":"44","author":"JK Jacobsen","year":"2011","unstructured":"Jacobsen JK, Jensen B, Skonberg C et al (2011) Time-course activities of Oct1, Mrp3, and cytochrome P450s in cultures of cryopreserved rat hepatocytes. Eur J Pharm Sci 44:427\u2013436","journal-title":"Eur J Pharm Sci"},{"key":"12_CR7","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1124\/dmd.108.023465","volume":"37","author":"K Abe","year":"2009","unstructured":"Abe K, Bridges AS, Brouwer KLR (2009) Use of sandwich-cultured human hepatocytes to predict biliary clearance of angiotensin II receptor blockers and HMG-CoA reductase inhibitors. Drug Metab Dispos 37:447\u2013452","journal-title":"Drug Metab Dispos"},{"key":"12_CR8","doi-asserted-by":"publisher","first-page":"983","DOI":"10.1124\/jpet.108.138073","volume":"326","author":"K Abe","year":"2008","unstructured":"Abe K, Bridges AS, Yue W et al (2008) In vitro biliary clearance of angiotensin II receptor blockers and 3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibitors in sandwich-cultured rat hepatocytes: comparison with in vivo biliary clearance. J Pharmacol Exp Ther 326:983\u2013990","journal-title":"J Pharmacol Exp Ther"},{"key":"12_CR9","doi-asserted-by":"publisher","first-page":"1085","DOI":"10.1124\/dmd.111.043489","volume":"40","author":"Y Bi","year":"2012","unstructured":"Bi Y, Kimoto E, Sevidal S et al (2012) In vitro evaluation of hepatic transporter-mediated clinical drug-drug interactions: hepatocyte model optimization and retrospective investigation. Drug Metab Dispos 40:1085\u20131092","journal-title":"Drug Metab Dispos"},{"key":"12_CR10","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1016\/j.taap.2013.10.032","volume":"274","author":"S Chatterjee","year":"2014","unstructured":"Chatterjee S, Richert L, Augustijns P et al (2014) Hepatocyte-based in vitro model for assessment of drug-induced cholestasis. Toxicol Appl Pharmacol 274:124\u2013136","journal-title":"Toxicol Appl Pharmacol"},{"key":"12_CR11","doi-asserted-by":"publisher","first-page":"589","DOI":"10.1517\/17425255.2013.773973","volume":"9","author":"T De Bruyn","year":"2013","unstructured":"De Bruyn T, Chatterjee S, Fattah S et al (2013) Sandwich-cultured hepatocytes: utility for in vitro exploration of hepatobiliary drug disposition and drug-induced hepatotoxicity. Expert Opin Drug Metab Toxicol 9:589\u2013616","journal-title":"Expert Opin Drug Metab Toxicol"},{"key":"12_CR12","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1093\/toxsci\/kfq294","volume":"118","author":"TKL Kiang","year":"2010","unstructured":"Kiang TKL, Teng XW, Karagiozov S et al (2010) Role of oxidative metabolism in the effect of valproic acid on markers of cell viability, necrosis, and oxidative stress in sandwich-cultured rat hepatocytes. Toxicol Sci 118:501\u2013509","journal-title":"Toxicol Sci"},{"key":"12_CR13","unstructured":"Keemink J, De Bruyn T, Augustijns P et al. (2014) Effect of cryopreservation on enzyme and transporter activities in suspended and sandwich cultured rat hepatocytes. Submitted"},{"key":"12_CR14","doi-asserted-by":"publisher","first-page":"270","DOI":"10.1016\/j.vascn.2012.07.004","volume":"66","author":"JS Halladay","year":"2012","unstructured":"Halladay JS, Wong S, Khojasteh SC et al (2012) An all-inclusive 96-well cytochrome P450 induction method: measuring enzyme activity, mRNA levels, protein levels, and cytotoxicity from one well using cryopreserved human hepatocytes. J Pharmacol Toxicol Methods 66:270\u2013275","journal-title":"J Pharmacol Toxicol Methods"},{"key":"12_CR15","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/j.tiv.2006.10.012","volume":"21","author":"RT Mingoia","year":"2007","unstructured":"Mingoia RT, Nabb DL, Yang CH et al (2007) Primary culture of rat hepatocytes in 96-well plates: effects of extracellular matrix configuration on cytochrome P450 enzyme activity and inducibility, and its application in in vitro cytotoxicity screening. Toxicol In Vitro 21: 165\u2013173","journal-title":"Toxicol In Vitro"},{"key":"12_CR16","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1016\/j.bioeng.2006.05.018","volume":"24","author":"I Jasmund","year":"2007","unstructured":"Jasmund I, Schwientek S, Acikgoz A et al (2007) The influence of medium composition and matrix on long-term cultivation of primary porcine and human hepatocytes. Biomol Eng 24:59\u201369","journal-title":"Biomol Eng"},{"key":"12_CR17","first-page":"1277","volume":"29","author":"P Annaert","year":"2001","unstructured":"Annaert P, Turncliff RZ, Booth CL et al (2001) P-glycoprotein-mediated in vitro biliary excretion in sandwich-cultured rat hepatocytes. Drug Metab Dispos 29:1277\u20131283","journal-title":"Drug Metab Dispos"},{"key":"12_CR18","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1111\/j.1525-1594.2010.01191.x","volume":"35","author":"JN Lu","year":"2011","unstructured":"Lu JN, Wang CC, Young TH (2011) The behaviors of long-term cryopreserved human hepatocytes on different biomaterials. Artif Organs 35:65\u201372","journal-title":"Artif Organs"},{"key":"12_CR19","doi-asserted-by":"publisher","first-page":"892","DOI":"10.1016\/j.tiv.2007.01.010","volume":"21","author":"AS Kienhuis","year":"2007","unstructured":"Kienhuis AS, Wortelboer HM, Maas WJ et al (2007) A sandwich-cultured rat hepatocyte system with increased metabolic competence evaluated by gene expression profiling. Toxicol In Vitro 21:892\u2013901","journal-title":"Toxicol In Vitro"},{"key":"12_CR20","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/S0169-409X(96)00418-8","volume":"22","author":"EL LeCluyse","year":"1996","unstructured":"LeCluyse EL, Bullock PL, Parkinson A (1996) Strategies for restoration and maintenance of normal hepatic structure and function in long-term cultures of rat hepatocytes. Adv Drug Deliv Rev 22:133\u2013186","journal-title":"Adv Drug Deliv Rev"},{"key":"12_CR21","doi-asserted-by":"publisher","first-page":"5927","DOI":"10.1016\/j.biomaterials.2009.07.022","volume":"30","author":"L Xia","year":"2009","unstructured":"Xia L, Ng S, Han R et al (2009) Laminar-flow immediate-overlay hepatocyte sandwich perfusion system for drug hepatotoxicity testing. Biomaterials 30:5927\u20135936","journal-title":"Biomaterials"},{"key":"12_CR22","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1016\/S0928-0987(01)00135-X","volume":"13","author":"EL LeCluyse","year":"2001","unstructured":"LeCluyse EL (2001) Human hepatocyte culture systems for the in vitro evaluation of cytochrome P450 expression and regulation. Eur J Pharm Sci 13:343\u2013368","journal-title":"Eur J Pharm Sci"},{"key":"12_CR23","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1021\/mp900227a","volume":"7","author":"B Swift","year":"2010","unstructured":"Swift B, Brouwer KLR (2010) Influence of seeding density and extracellular matrix on bile Acid transport and mrp4 expression in sandwich-cultured mouse hepatocytes. Mol Pharm 7:491\u2013500","journal-title":"Mol Pharm"},{"key":"12_CR24","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1124\/dmd.110.034298","volume":"39","author":"E Kimoto","year":"2011","unstructured":"Kimoto E, Chupka J, Xiao Y et al (2011) Characterization of digoxin uptake in sandwich-cultured human hepatocytes. Drug Metab Dispos 39:47\u201353","journal-title":"Drug Metab Dispos"},{"key":"12_CR25","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1124\/jpet.113.207472","volume":"347","author":"ND Pfeifer","year":"2013","unstructured":"Pfeifer ND, Yang K, Brouwer KLR (2013) Hepatic basolateral efflux contributes significantly to rosuvastatin disposition I: characterization of basolateral versus biliary clearance using a novel protocol in sandwich-cultured hepatocytes. J Pharmacol Exp Ther 347:727\u2013736","journal-title":"J Pharmacol Exp Ther"},{"key":"12_CR26","doi-asserted-by":"publisher","first-page":"1872","DOI":"10.1002\/jps.23933","volume":"103","author":"T De Bruyn","year":"2014","unstructured":"De Bruyn T, Sempels W, Snoeys J et al (2014) Confocal imaging with a fluorescent bile acid analogue closely mimicking hepatic taurocholate disposition. J Pharm Sci 103:1872\u20131881","journal-title":"J Pharm Sci"},{"key":"12_CR27","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1124\/jpet.110.177220","volume":"337","author":"GZ Yan","year":"2011","unstructured":"Yan GZ, Brouwer KLR, Pollack GM et al (2011) Mechanisms underlying differences in systemic exposure of structurally similar active metabolites: comparison of two preclinical hepatic models. J Pharmacol Exp Ther 337:503\u2013512","journal-title":"J Pharmacol Exp Ther"},{"key":"12_CR28","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1124\/dmd.111.042275","volume":"40","author":"O Schaefer","year":"2012","unstructured":"Schaefer O, Ohtsuki S, Kawakami H et al (2012) Absolute quantification and differential expression of drug transporters, cytochrome P450 enzymes, and UDP-glucuronosyltransferases in cultured primary human hepatocytes. Drug Metab Dispos 40:93\u2013103","journal-title":"Drug Metab Dispos"},{"key":"12_CR29","doi-asserted-by":"publisher","first-page":"1070","DOI":"10.1124\/dmd.110.037523","volume":"39","author":"BJ Kirby","year":"2011","unstructured":"Kirby BJ, Collier AC, Kharasch ED et al (2011) Complex drug interactions of HIV protease inhibitors 1: inactivation, induction, and inhibition of cytochrome P450 3A by ritonavir or nelfinavir. Drug Metab Dispos 39:1070\u20131078","journal-title":"Drug Metab Dispos"},{"key":"12_CR30","doi-asserted-by":"publisher","first-page":"570","DOI":"10.1152\/ajpgi.00542.2007","volume":"295","author":"R Govindarajan","year":"2008","unstructured":"Govindarajan R, Endres CJ, Whittington D et al (2008) Expression and hepatobiliary transport characteristics of the concentrative and equilibrative nucleoside transporters in sandwich-cultured human hepatocytes. Am J Physiol Gastrointest Liver Physiol 295:570\u2013580","journal-title":"Am J Physiol Gastrointest Liver Physiol"}],"container-title":["Methods in Molecular Biology","Protocols in In Vitro Hepatocyte Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-4939-2074-7_12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T16:11:14Z","timestamp":1676909474000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-4939-2074-7_12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11,3]]},"ISBN":["9781493920730","9781493920747"],"references-count":30,"URL":"https:\/\/doi.org\/10.1007\/978-1-4939-2074-7_12","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,11,3]]},"assertion":[{"value":"3 November 2014","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}