{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T18:04:52Z","timestamp":1794506692831,"version":"4.9.1"},"publisher-location":"New York, NY","reference-count":33,"publisher":"Springer New York","isbn-type":[{"value":"9781493928538","type":"print"},{"value":"9781493928545","type":"electronic"}],"license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016]]},"DOI":"10.1007\/978-1-4939-2854-5_12","type":"book-chapter","created":{"date-parts":[[2015,10,14]],"date-time":"2015-10-14T05:51:29Z","timestamp":1444801889000},"page":"131-143","source":"Crossref","is-referenced-by-count":6,"title":["Experimental Evolution of Escherichia coli Persister Levels Using Cyclic Antibiotic Treatments"],"prefix":"10.1007","author":[{"given":"Bram","family":"Van den Bergh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joran E.","family":"Michiels","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Michiels","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","reference":[{"key":"12_CR1","doi-asserted-by":"publisher","first-page":"632","DOI":"10.1016\/j.chom.2013.05.009","volume":"13","author":"NR Cohen","year":"2013","unstructured":"Cohen NR, Lobritz MA, Collins JJ (2013) Microbial persistence and the road to drug resistance. Cell Host Microbe 13:632\u2013642","journal-title":"Cell Host Microbe"},{"key":"12_CR2","doi-asserted-by":"publisher","first-page":"1622","DOI":"10.1126\/science.1099390","volume":"305","author":"NQ Balaban","year":"2004","unstructured":"Balaban NQ, Merrin J, Chait R et al (2004) Bacterial persistence as a phenotypic switch. Science 305:1622\u20131625","journal-title":"Science"},{"key":"12_CR3","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1146\/annurev.micro.112408.134306","volume":"64","author":"K Lewis","year":"2010","unstructured":"Lewis K (2010) Persister cells. Annu Rev Microbiol 64:357\u2013372","journal-title":"Annu Rev Microbiol"},{"key":"12_CR4","doi-asserted-by":"publisher","first-page":"699","DOI":"10.1099\/jmm.0.030932-0","volume":"60","author":"M Fauvart","year":"2011","unstructured":"Fauvart M, De Groote VN, Michiels J (2011) Role of persister cells in chronic infections: clinical relevance and perspectives on anti-persister therapies. J Med Microbiol 60:699\u2013709","journal-title":"J Med Microbiol"},{"key":"12_CR5","doi-asserted-by":"publisher","first-page":"5173","DOI":"10.1128\/AAC.01751-09","volume":"54","author":"J Manuel","year":"2010","unstructured":"Manuel J, Zhanel GG, de Kievit T (2010) Cadaverine suppresses persistence to carboxypenicillins in Pseudomonas aeruginosa PAO1. Antimicrob Agents Chemother 54:5173\u20135179","journal-title":"Antimicrob Agents Chemother"},{"key":"12_CR6","doi-asserted-by":"publisher","first-page":"2092","DOI":"10.1128\/AAC.00052-07","volume":"51","author":"Y Li","year":"2007","unstructured":"Li Y, Zhang Y (2007) PhoU is a persistence switch involved in persister formation and tolerance to multiple antibiotics and stresses in Escherichia coli. Antimicrob Agents Chemother 51:2092\u20132099","journal-title":"Antimicrob Agents Chemother"},{"key":"12_CR7","doi-asserted-by":"publisher","first-page":"12740","DOI":"10.1073\/pnas.1205124109","volume":"109","author":"HS Girgis","year":"2012","unstructured":"Girgis HS, Harris K, Tavazoie S (2012) Large mutational target size for rapid emergence of bacterial persistence. Proc Natl Acad Sci U S A 109:12740\u201312745","journal-title":"Proc Natl Acad Sci U S A"},{"key":"12_CR8","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1111\/j.1574-6968.2009.01657.x","volume":"297","author":"VN De Groote","year":"2009","unstructured":"De Groote VN, Verstraeten N, Fauvart M et al (2009) Novel persistence genes in Pseudomonas aeruginosa identified by high-throughput screening. FEMS Microbiol Lett 297:73\u201379","journal-title":"FEMS Microbiol Lett"},{"key":"12_CR9","doi-asserted-by":"publisher","first-page":"2265","DOI":"10.1128\/JB.06707-11","volume":"194","author":"V Leung","year":"2012","unstructured":"Leung V, Levesque CM, L\u00e9vesque CM (2012) A stress-inducible quorum-sensing peptide mediates the formation of persister cells with noninherited multidrug tolerance. J Bacteriol 194:2265\u20132274","journal-title":"J Bacteriol"},{"key":"12_CR10","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/j.femsle.2004.11.049","volume":"243","author":"Y Hu","year":"2005","unstructured":"Hu Y, Coates ARM (2005) Transposon mutagenesis identifies genes which control antimicrobial drug tolerance in stationary-phase Escherichia coli. FEMS Microbiol Lett 243:117\u2013124","journal-title":"FEMS Microbiol Lett"},{"key":"12_CR11","doi-asserted-by":"publisher","first-page":"12275","DOI":"10.1073\/pnas.1003219107","volume":"107","author":"N Dhar","year":"2010","unstructured":"Dhar N, McKinney JD (2010) Mycobacterium tuberculosis persistence mutants identified by screening in isoniazid-treated mice. Proc Natl Acad Sci USA 107:12275\u201312280","journal-title":"Proc Natl Acad Sci USA"},{"key":"12_CR12","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1111\/j.1574-6968.2009.01857.x","volume":"303","author":"C Ma","year":"2010","unstructured":"Ma C, Sim S, Shi W et al (2010) Energy production genes sucB and ubiF are involved in persister survival and tolerance to multiple antibiotics and stresses in Escherichia coli. FEMS Microbiol Lett 303:33\u201340","journal-title":"FEMS Microbiol Lett"},{"key":"12_CR13","doi-asserted-by":"publisher","first-page":"2718","DOI":"10.1128\/AAC.00144-08","volume":"52","author":"S Hansen","year":"2008","unstructured":"Hansen S, Lewis K, Vuli\u0107 M (2008) Role of global regulators and nucleotide metabolism in antibiotic tolerance in Escherichia coli. Antimicrob Agents Chemother 52:2718\u20132726","journal-title":"Antimicrob Agents Chemother"},{"key":"12_CR14","doi-asserted-by":"publisher","first-page":"5136","DOI":"10.1128\/JB.00369-06","volume":"188","author":"AL Spoering","year":"2006","unstructured":"Spoering AL, Vulic M, Lewis K et al (2006) GlpD and PlsB participate in persister cell formation in Escherichia coli. J Bacteriol 188:5136\u20135144","journal-title":"J Bacteriol"},{"key":"12_CR15","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/j.molcel.2013.08.045","volume":"52","author":"E Germain","year":"2013","unstructured":"Germain E, Castro-Roa D, Zenkin N et al (2013) Molecular mechanism of bacterial persistence by HipA. Mol Cell 52:248\u2013254","journal-title":"Mol Cell"},{"key":"12_CR16","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1186\/1471-2180-6-53","volume":"6","author":"D Shah","year":"2006","unstructured":"Shah D, Zhang Z, Khodursky A et al (2006) Persisters: a distinct physiological state of E. coli. BMC Microbiol 6:53","journal-title":"BMC Microbiol"},{"key":"12_CR17","doi-asserted-by":"publisher","first-page":"e00100","DOI":"10.1128\/mBio.00100-11","volume":"2","author":"I Keren","year":"2011","unstructured":"Keren I, Minami S, Rubin E et al (2011) Characterization and transcriptome analysis of Mycobacterium tuberculosis persisters. MBio 2:e00100\u2013e00111","journal-title":"MBio"},{"key":"12_CR18","doi-asserted-by":"publisher","first-page":"8172","DOI":"10.1128\/JB.186.24.8172-8180.2004","volume":"186","author":"I Keren","year":"2004","unstructured":"Keren I, Shah D, Spoering A et al (2004) Specialized persister cells and the mechanism of multidrug tolerance in Escherichia coli. J Bacteriol 186:8172\u20138180","journal-title":"J Bacteriol"},{"key":"12_CR19","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058943","volume":"8","author":"H Van Acker","year":"2013","unstructured":"Van Acker H, Sass A, Bazzini S et al (2013) Biofilm-grown Burkholderia cepacia complex cells survive antibiotic treatment by avoiding production of reactive oxygen species. PLoS One 8:e58943","journal-title":"PLoS One"},{"key":"12_CR20","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1016\/j.tim.2012.08.009","volume":"20","author":"CI Kint","year":"2012","unstructured":"Kint CI, Verstraeten N, Fauvart M et al (2012) New-found fundamentals of bacterial persistence. Trends Microbiol 20:577\u2013585","journal-title":"Trends Microbiol"},{"key":"12_CR21","doi-asserted-by":"publisher","first-page":"70","DOI":"10.3389\/fmicb.2014.00070","volume":"5","author":"SM Amato","year":"2014","unstructured":"Amato SM, Fazen CH, Henry TC et al (2014) The role of metabolism in bacterial persistence. Front Microbiol 5:70","journal-title":"Front Microbiol"},{"key":"12_CR22","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1038\/nrmicro3076","volume":"11","author":"NQ Balaban","year":"2013","unstructured":"Balaban NQ, Gerdes K, Lewis K et al (2013) A problem of persistence: still more questions than answers? Nat Rev Microbiol 11:587\u2013591","journal-title":"Nat Rev Microbiol"},{"key":"12_CR23","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1128\/AEM.05773-11","volume":"78","author":"IK Blaby","year":"2012","unstructured":"Blaby IK, Lyons BJ, Wroclawska-Hughes E et al (2012) Experimental evolution of a facultative thermophile from a mesophilic ancestor. Appl Environ Microbiol 78:144\u2013155","journal-title":"Appl Environ Microbiol"},{"key":"12_CR24","doi-asserted-by":"publisher","first-page":"1243","DOI":"10.1038\/nature08480","volume":"461","author":"JE Barrick","year":"2009","unstructured":"Barrick JE, Yu DS, Yoon SH et al (2009) Genome evolution and adaptation in a long-term experiment with Escherichia coli. Nature 461:1243\u20131247","journal-title":"Nature"},{"key":"12_CR25","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1128\/AAC.00860-09","volume":"54","author":"MD Lafleur","year":"2010","unstructured":"Lafleur MD, Qi Q, Lewis K (2010) Patients with long-term oral carriage harbor high-persister mutants of Candida albicans. Antimicrob Agents Chemother 54:39\u201344","journal-title":"Antimicrob Agents Chemother"},{"key":"12_CR26","doi-asserted-by":"publisher","first-page":"6191","DOI":"10.1128\/JB.01651-09","volume":"192","author":"LR Mulcahy","year":"2010","unstructured":"Mulcahy LR, Burns JL, Lory S et al (2010) Emergence of Pseudomonas aeruginosa strains producing high levels of persister cells in patients with cystic fibrosis. J Bacteriol 192:6191\u20136199","journal-title":"J Bacteriol"},{"key":"12_CR27","doi-asserted-by":"publisher","first-page":"418","DOI":"10.1038\/nature13469","volume":"513","author":"O Fridman","year":"2014","unstructured":"Fridman O, Goldberg A, Ronin I et al (2014) Optimization of lag time underlies antibiotic tolerance in evolved bacterial populations. Nature 513:418\u2013421","journal-title":"Nature"},{"key":"12_CR28","doi-asserted-by":"publisher","first-page":"1600","DOI":"10.1126\/science.1119623","volume":"311","author":"J Yu","year":"2006","unstructured":"Yu J, Xiao J, Ren X et al (2006) Probing gene expression in live cells, one protein molecule at a time. Science 311:1600\u20131603","journal-title":"Science"},{"issue":"Suppl 1","key":"12_CR29","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1093\/jac\/48.suppl_1.5","volume":"48","author":"JM Andrews","year":"2001","unstructured":"Andrews JM (2001) Determination of minimum inhibitory concentrations. J Antimicrob Chemother 48(Suppl 1):5\u201316","journal-title":"J Antimicrob Chemother"},{"key":"12_CR30","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1038\/nprot.2007.521","volume":"3","author":"I Wiegand","year":"2008","unstructured":"Wiegand I, Hilpert K, Hancock REW (2008) Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc 3:163\u2013175","journal-title":"Nat Protoc"},{"key":"12_CR31","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1111\/2049-632X.12174","volume":"71","author":"V Liebens","year":"2014","unstructured":"Liebens V, Defraine V, Van der Leyden A et al (2014) A putative de-N-acetylase of the PIG-L superfamily affects fluoroquinolone tolerance in Pseudomonas aeruginosa. Pathog Dis 71:39\u201354","journal-title":"Pathog Dis"},{"key":"12_CR32","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1093\/jac\/dkg269","volume":"52","author":"K Drlica","year":"2003","unstructured":"Drlica K (2003) The mutant selection window and antimicrobial resistance. J Antimicrob Chemother 52:11\u201317","journal-title":"J Antimicrob Chemother"},{"key":"12_CR33","doi-asserted-by":"publisher","first-page":"827","DOI":"10.1038\/nrg3564","volume":"14","author":"JE Barrick","year":"2013","unstructured":"Barrick JE, Lenski RE (2013) Genome dynamics during experimental evolution. Nat Rev Genet 14:827\u2013839","journal-title":"Nat Rev Genet"}],"container-title":["Methods in Molecular Biology","Bacterial Persistence"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-4939-2854-5_12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T14:31:24Z","timestamp":1675261884000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-4939-2854-5_12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"ISBN":["9781493928538","9781493928545"],"references-count":33,"URL":"https:\/\/doi.org\/10.1007\/978-1-4939-2854-5_12","relation":{"is-part-of":[{"id-type":"doi","id":"https:\/\/doi.org\/10.1007\/978-1-4939-2854-5","asserted-by":"object"}]},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016]]}}}