{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T14:52:01Z","timestamp":1778683921337,"version":"3.51.4"},"publisher-location":"New York, NY","reference-count":14,"publisher":"Springer New York","isbn-type":[{"value":"9781493930661","type":"print"},{"value":"9781493930678","type":"electronic"}],"license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016]]},"DOI":"10.1007\/978-1-4939-3067-8_6","type":"book-chapter","created":{"date-parts":[[2015,10,13]],"date-time":"2015-10-13T12:13:11Z","timestamp":1444738391000},"page":"99-108","source":"Crossref","is-referenced-by-count":14,"title":["Studying Isoform-Specific mRNA Recruitment to Polyribosomes with Frac-seq"],"prefix":"10.1007","author":[{"given":"Rocio T.","family":"Martinez-Nunez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy R.","family":"Sanford","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","reference":[{"key":"6_CR1","doi-asserted-by":"publisher","first-page":"654","DOI":"10.1073\/pnas.49.5.654","volume":"49","author":"S Penman","year":"1963","unstructured":"Penman S et al (1963) Polyribosomes in normal and poliovirus-infected Hela cells and their relationship to messenger-RNA. Proc Natl Acad Sci U S A 49:654\u2013662","journal-title":"Proc Natl Acad Sci U S A"},{"key":"6_CR2","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1091\/mbc.6.5.611","volume":"6","author":"AL Atkin","year":"1995","unstructured":"Atkin AL et al (1995) The majority of yeast UPF1 co-localizes with polyribosomes in the cytoplasm. Mol Biol Cell 6:611\u2013625","journal-title":"Mol Biol Cell"},{"key":"6_CR3","doi-asserted-by":"publisher","first-page":"2523","DOI":"10.1093\/hmg\/ddl173","volume":"15","author":"TF Satterfield","year":"2006","unstructured":"Satterfield TF, Pallanck LJ (2006) Ataxin-2 and its Drosophila homolog, ATX2, physically assemble with polyribosomes. Hum Mol Genet 15:2523\u20132532","journal-title":"Hum Mol Genet"},{"key":"6_CR4","doi-asserted-by":"publisher","first-page":"624","DOI":"10.1104\/pp.105.059477","volume":"138","author":"ME Zanetti","year":"2005","unstructured":"Zanetti ME et al (2005) Immunopurification of polyribosomal complexes of Arabidopsis for global analysis of gene expression. Plant Physiol 138:624\u2013635","journal-title":"Plant Physiol"},{"key":"6_CR5","first-page":"1948","volume":"2010","author":"AM Esposito","year":"2010","unstructured":"Esposito AM et al (2010) Eukaryotic polyribosome profile analysis. J Vis Exp 2010:1948","journal-title":"J Vis Exp"},{"key":"6_CR6","doi-asserted-by":"publisher","first-page":"1534","DOI":"10.1038\/nprot.2012.086","volume":"7","author":"NT Ingolia","year":"2012","unstructured":"Ingolia NT et al (2012) The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments. Nat Protoc 7:1534\u20131550","journal-title":"Nat Protoc"},{"key":"6_CR7","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/j.cell.2006.01.044","volume":"125","author":"T Kislinger","year":"2006","unstructured":"Kislinger T et al (2006) Global survey of organ and organelle protein expression in mouse: combined proteomic and transcriptomic profiling. Cell 125:173\u2013186","journal-title":"Cell"},{"issue":"4","key":"6_CR8","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1074\/mcp.M200001-MCP200","volume":"1","author":"Timothy J. Griffin","year":"2002","unstructured":"Griffin TJ et al (2002) Complementary profiling of gene expression at the transcriptome and proteome levels in Saccharomyces cerevisiae. Mol Cell Proteomics 1:323\u20133339","journal-title":"Molecular & Cellular Proteomics"},{"issue":"12","key":"6_CR9","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1038\/nmeth.1528","volume":"7","author":"Yarden Katz","year":"2010","unstructured":"Katz Y et al (2010) Analysis and design of RNA sequencing experiments for identifying isoform regulation. Nat Methods 7:1009\u2013101510","journal-title":"Nature Methods"},{"key":"6_CR10","doi-asserted-by":"crossref","unstructured":"Li B, Dewey CN (2011) RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome. BMC Bioinformatics 12:32311","DOI":"10.1186\/1471-2105-12-323"},{"issue":"3","key":"6_CR11","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1038\/nprot.2012.016","volume":"7","author":"Cole Trapnell","year":"2012","unstructured":"Trapnell C et al (2012) Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. Nat Protoc 7:562\u2013578","journal-title":"Nature Protocols"},{"issue":"10","key":"6_CR12","doi-asserted-by":"crossref","first-page":"1615","DOI":"10.1101\/gr.148585.112","volume":"23","author":"T. Sterne-Weiler","year":"2013","unstructured":"Sterne-Weiler T et al (2013) Frac-seq reveals isoform-specific recruitment to polyribosomes. Genome Res 23:1615\u2013162313","journal-title":"Genome Research"},{"key":"6_CR13","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1038\/nmeth.1923","volume":"9","author":"B Langmead","year":"2012","unstructured":"Langmead B, Salzberg SL (2012) Fast gapped-read alignment with Bowtie 2. Nat Methods 9:357\u2013359","journal-title":"Nat Methods"},{"key":"6_CR14","doi-asserted-by":"publisher","first-page":"1105","DOI":"10.1093\/bioinformatics\/btp120","volume":"25","author":"C Trapnell","year":"2009","unstructured":"Trapnell C, Pachter L, Salzberg SL (2009) TopHat: discovering splice junctions with RNA-Seq. Bioinformatics 25:1105\u20131111","journal-title":"Bioinformatics"}],"container-title":["Methods in Molecular Biology","Post-Transcriptional Gene Regulation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-4939-3067-8_6","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,31]],"date-time":"2019-05-31T01:37:10Z","timestamp":1559266630000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-4939-3067-8_6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"ISBN":["9781493930661","9781493930678"],"references-count":14,"URL":"https:\/\/doi.org\/10.1007\/978-1-4939-3067-8_6","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016]]}}}