{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T05:11:31Z","timestamp":1774069891113,"version":"3.50.1"},"publisher-location":"New York, NY","reference-count":31,"publisher":"Springer New York","isbn-type":[{"value":"9781493933884","type":"print"},{"value":"9781493933891","type":"electronic"}],"license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016]]},"DOI":"10.1007\/978-1-4939-3389-1_41","type":"book-chapter","created":{"date-parts":[[2016,4,14]],"date-time":"2016-04-14T11:22:46Z","timestamp":1460632966000},"page":"635-649","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Preparation of Multifunctional Liposomes as a Stable Vaccine Delivery-Adjuvant System by Procedure of Emulsification-Lyophilization"],"prefix":"10.1007","author":[{"given":"Ning","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,4,14]]},"reference":[{"issue":"1","key":"41_CR1","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1016\/j.vaccine.2012.10.103","volume":"31","author":"S Ozawa","year":"2012","unstructured":"Ozawa S, Mirelman A, Stack ML, Walker DG, Levine OS (2012) Cost-effectiveness and economic benefits of vaccines in low- and middle-income countries: a systematic review. Vaccine 31(1):96\u2013108. doi:10.1016\/j.vaccine.2012.10.103, S0264-410X(12)01576-9 [pii]","journal-title":"Vaccine"},{"issue":"3","key":"41_CR2","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1002\/cmdc.201200487","volume":"8","author":"PM Moyle","year":"2013","unstructured":"Moyle PM, Toth I (2013) Modern subunit vaccines: development, components, and research opportunities. ChemMedChem 8(3):360\u2013376. doi:10.1002\/cmdc.201200487","journal-title":"ChemMedChem"},{"key":"41_CR3","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1016\/j.colsurfb.2013.01.041","volume":"106","author":"TT Pham","year":"2013","unstructured":"Pham TT, Barratt G, Michel JP, Loiseau PM, Saint-Pierre-Chazalet M (2013) Interactions of antileishmanial drugs with monolayers of lipids used in the development of amphotericin B-miltefosine-loaded nanocochleates. Colloids Surf B Biointerfaces 106:224\u2013233. doi:10.1016\/j.colsurfb.2013.01.041","journal-title":"Colloids Surf B Biointerfaces"},{"issue":"4 Suppl","key":"41_CR4","doi-asserted-by":"publisher","first-page":"S5","DOI":"10.1038\/nm1209","volume":"11","author":"SA Plotkin","year":"2005","unstructured":"Plotkin SA (2005) Vaccines: past, present and future. Nat Med 11(4 Suppl):S5\u2013S11. doi:10.1038\/nm1209, nm1209 [pii]","journal-title":"Nat Med"},{"issue":"4","key":"41_CR5","doi-asserted-by":"publisher","first-page":"385","DOI":"10.2174\/1567201043334588","volume":"1","author":"PM Moyle","year":"2004","unstructured":"Moyle PM, McGeary RP, Blanchfield JT, Toth I (2004) Mucosal immunisation: adjuvants and delivery systems. Curr Drug Deliv 1(4):385\u2013396","journal-title":"Curr Drug Deliv"},{"issue":"4","key":"41_CR6","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1586\/erv.11.21","volume":"10","author":"VE Schijns","year":"2011","unstructured":"Schijns VE, Lavelle EC (2011) Trends in vaccine adjuvants. Expert Rev Vaccines 10(4):539\u2013550. doi:10.1586\/erv.11.21","journal-title":"Expert Rev Vaccines"},{"issue":"9","key":"41_CR7","doi-asserted-by":"publisher","first-page":"1139","DOI":"10.1586\/erv.12.81","volume":"11","author":"A Gebril","year":"2012","unstructured":"Gebril A, Alsaadi M, Acevedo R, Mullen AB, Ferro VA (2012) Optimizing efficacy of mucosal vaccines. Expert Rev Vaccines 11(9):1139\u20131155. doi:10.1586\/erv.12.81","journal-title":"Expert Rev Vaccines"},{"issue":"21","key":"41_CR8","doi-asserted-by":"publisher","first-page":"2235","DOI":"10.2174\/092986707781696609","volume":"14","author":"RE O\u2019Hehir","year":"2007","unstructured":"O\u2019Hehir RE, Sandrini A, Anderson GP, Rolland JM (2007) Sublingual allergen immunotherapy: immunological mechanisms and prospects for refined vaccine preparation. Curr Med Chem 14(21):2235\u20132244","journal-title":"Curr Med Chem"},{"issue":"1","key":"41_CR9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.cyto.2010.12.014","volume":"54","author":"MN Kweon","year":"2011","unstructured":"Kweon MN (2011) Sublingual mucosa: a new vaccination route for systemic and mucosal immunity. Cytokine 54(1):1\u20135. doi:10.1016\/j.cyto.2010.12.014, S1043-4666(10)00797-0 [pii]","journal-title":"Cytokine"},{"issue":"5","key":"41_CR10","doi-asserted-by":"publisher","first-page":"165","DOI":"10.4110\/in.2012.12.5.165","volume":"12","author":"SH Kim","year":"2012","unstructured":"Kim SH, Lee KY, Jang YS (2012) Mucosal immune system and M cell-targeting strategies for oral mucosal vaccination. Immune Netw 12(5):165\u2013175. doi:10.4110\/in.2012.12.5.165","journal-title":"Immune Netw"},{"issue":"6","key":"41_CR11","doi-asserted-by":"publisher","first-page":"733","DOI":"10.1586\/erv.12.35","volume":"11","author":"CR Alving","year":"2012","unstructured":"Alving CR, Rao M, Steers NJ, Matyas GR, Mayorov AV (2012) Liposomes containing lipid A: an effective, safe, generic adjuvant system for synthetic vaccines. Expert Rev Vaccines 11(6):733\u2013744. doi:10.1586\/erv.12.35","journal-title":"Expert Rev Vaccines"},{"issue":"6","key":"41_CR12","doi-asserted-by":"publisher","first-page":"703","DOI":"10.1517\/17425247.5.6.703","volume":"5","author":"JM Irache","year":"2008","unstructured":"Irache JM, Salman HH, Gamazo C, Espuelas S (2008) Mannose-targeted systems for the delivery of therapeutics. Expert Opin Drug Deliv 5(6):703\u2013724. doi:10.1517\/17425247.5.6.703","journal-title":"Expert Opin Drug Deliv"},{"issue":"1\u20132","key":"41_CR13","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.ijpharm.2014.04.002","volume":"468","author":"N Wang","year":"2014","unstructured":"Wang N, Wang T, Zhang M, Chen R, Deng Y (2014) Using procedure of emulsification-lyophilization to form lipid A-incorporating cochleates as an effective oral mucosal vaccine adjuvant-delivery system (VADS). Int J Pharm 468(1\u20132):39\u201349. doi:10.1016\/j.ijpharm.2014.04.002, S0378-5173(14)00230-0 [pii]","journal-title":"Int J Pharm"},{"key":"41_CR14","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1016\/j.ejpb.2014.04.007","volume":"88","author":"N Wang","year":"2014","unstructured":"Wang N, Wang T, Zhang M, Chen R, Niu R, Deng Y (2014) Mannose derivative and lipid A dually decorated cationic liposomes as an effective cold chain free oral mucosal vaccine adjuvant-delivery system. Eur J Pharm Biopharm 88:194\u2013206. doi:10.1016\/j.ejpb.2014.04.007, S0939-6411(14)00124-6 [pii]","journal-title":"Eur J Pharm Biopharm"},{"issue":"2","key":"41_CR15","doi-asserted-by":"publisher","first-page":"222","DOI":"10.1016\/j.bbamem.2006.01.023","volume":"1758","author":"T Wang","year":"2006","unstructured":"Wang T, Deng Y, Geng Y, Gao Z, Zou J, Wang Z (2006) Preparation of submicron unilamellar liposomes by freeze-drying double emulsions. Biochim Biophys Acta 1758(2):222\u2013231. doi:10.1016\/j.bbamem.2006.01.023, S0005-2736(06)00033-2 [pii]","journal-title":"Biochim Biophys Acta"},{"issue":"3","key":"41_CR16","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1080\/08982100902788390","volume":"19","author":"T Wang","year":"2009","unstructured":"Wang T, Wang N, Jin X, Zhang K, Li T (2009) A novel procedure for preparation of submicron liposomes-lyophilization of oil-in-water emulsions. J Liposome Res 19(3):231\u2013240. doi:10.1080\/08982100902788390, 909232172 [pii]","journal-title":"J Liposome Res"},{"issue":"1\u20132","key":"41_CR17","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1016\/j.ejps.2011.06.015","volume":"44","author":"T Wang","year":"2011","unstructured":"Wang T, Wang N, Song H, Xi X, Wang J, Hao A, Li T (2011) Preparation of an anhydrous reverse micelle delivery system to enhance oral bioavailability and anti-diabetic efficacy of berberine. Eur J Pharm Sci 44(1\u20132):127\u2013135. doi:10.1016\/j.ejps.2011.06.015, S0928-0987(11)00218-1 [pii]","journal-title":"Eur J Pharm Sci"},{"issue":"2","key":"41_CR18","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1016\/j.chemphyslip.2010.12.005","volume":"164","author":"T Wang","year":"2011","unstructured":"Wang T, Wang N, Sun W, Li T (2011) Preparation of submicron liposomes exhibiting efficient entrapment of drugs by freeze-drying water-in-oil emulsions. Chem Phys Lipids 164(2):151\u2013157. doi:10.1016\/j.chemphyslip.2010.12.005, S0009-3084(10)00391-9 [pii]","journal-title":"Chem Phys Lipids"},{"issue":"38","key":"41_CR19","doi-asserted-by":"publisher","first-page":"5637","DOI":"10.1016\/j.vaccine.2012.06.087","volume":"30","author":"BY Lee","year":"2012","unstructured":"Lee BY, Cakouros BE, Assi TM, Connor DL, Welling J, Kone S, Djibo A, Wateska AR, Pierre L, Brown ST (2012) The impact of making vaccines thermostable in Niger\u2019s vaccine supply chain. Vaccine 30(38):5637\u20135643. doi:10.1016\/j.vaccine.2012.06.087, S0264-410X(12)00977-2 [pii]","journal-title":"Vaccine"},{"issue":"2","key":"41_CR20","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1163\/156856298X00479","volume":"9","author":"S Ge","year":"1998","unstructured":"Ge S, Kojio K, Takahara A, Kajiyama T (1998) Bovine serum albumin adsorption onto immobilized organotrichlorosilane surface: influence of the phase separation on protein adsorption patterns. J Biomater Sci Polym Ed 9(2):131\u2013150","journal-title":"J Biomater Sci Polym Ed"},{"key":"41_CR21","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1007\/978-1-60761-585-9_6","volume":"626","author":"J Haensler","year":"2010","unstructured":"Haensler J (2010) Liposomal adjuvants: preparation and formulation with antigens. Methods Mol Biol 626:73\u201390. doi:10.1007\/978-1-60761-585-9_6","journal-title":"Methods Mol Biol"},{"issue":"31","key":"41_CR22","doi-asserted-by":"publisher","first-page":"3955","DOI":"10.1016\/j.vaccine.2014.05.010","volume":"32","author":"A Ruyra","year":"2014","unstructured":"Ruyra A, Cano-Sarabia M, Garcia-Valtanen P, Yero D, Gibert I, Mackenzie SA, Estepa A, Maspoch D, Roher N (2014) Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS\/dsRNA-loaded nanoliposomes. Vaccine 32(31):3955\u20133962. doi:10.1016\/j.vaccine.2014.05.010","journal-title":"Vaccine"},{"issue":"8","key":"41_CR23","doi-asserted-by":"publisher","first-page":"1084","DOI":"10.1016\/j.vaccine.2014.10.078","volume":"33","author":"Geert Leroux-Roels","year":"2015","unstructured":"Leroux-Roels G, Van Belle P, Vandepapeliere P, Horsmans Y, Janssens M, Carletti I, Garcon N, Wettendorff M, Van Mechelen M (2014) Vaccine adjuvant systems containing monophosphoryl lipid A and QS-21 induce strong humoral and cellular immune responses against hepatitis B surface antigen which persist for at least 4 years after vaccination. doi:10.1016\/j.vaccine.2014.10.078","journal-title":"Vaccine"},{"issue":"3","key":"41_CR24","doi-asserted-by":"publisher","first-page":"334","DOI":"10.1016\/j.nano.2008.12.004","volume":"5","author":"V Jain","year":"2009","unstructured":"Jain V, Vyas SP, Kohli DV (2009) Well-defined and potent liposomal hepatitis B vaccines adjuvanted with lipophilic MDP derivatives. Nanomedicine 5(3):334\u2013344. doi:10.1016\/j.nano.2008.12.004","journal-title":"Nanomedicine"},{"issue":"15\u201316","key":"41_CR25","doi-asserted-by":"publisher","first-page":"1903","DOI":"10.1016\/j.vaccine.2003.11.008","volume":"22","author":"C Foged","year":"2004","unstructured":"Foged C, Arigita C, Sundblad A, Jiskoot W, Storm G, Frokjaer S (2004) Interaction of dendritic cells with antigen-containing liposomes: effect of bilayer composition. Vaccine 22(15\u201316):1903\u20131913. doi:10.1016\/j.vaccine.2003.11.008","journal-title":"Vaccine"},{"issue":"9","key":"41_CR26","doi-asserted-by":"publisher","first-page":"2252","DOI":"10.1016\/j.bbamem.2012.04.002","volume":"1818","author":"A Stimac","year":"2012","unstructured":"Stimac A, Segota S, Dutour Sikiric M, Ribic R, Frkanec L, Svetlicic V, Tomic S, Vranesic B, Frkanec R (2012) Surface modified liposomes by mannosylated conjugates anchored via the adamantyl moiety in the lipid bilayer. Biochim Biophys Acta 1818(9):2252\u20132259. doi:10.1016\/j.bbamem.2012.04.002","journal-title":"Biochim Biophys Acta"},{"issue":"5","key":"41_CR27","doi-asserted-by":"publisher","first-page":"884","DOI":"10.1021\/bc034068q","volume":"14","author":"Eric Perouzel","year":"2003","unstructured":"Perouzel E, Jorgensen MR, Keller M, Miller AD (2003) Synthesis and formulation of neoglycolipids for the functionalization of liposomes and lipoplexes.Bioconjug Chem 14(5):884-898. doi:10.1021\/bc034068q","journal-title":"Bioconjugate Chemistry"},{"issue":"1\u20132","key":"41_CR28","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/S0009-3084(01)00178-5","volume":"113","author":"C Carrion","year":"2001","unstructured":"Carrion C, Domingo JC, de Madariaga MA (2001) Preparation of long-circulating immunoliposomes using PEG-cholesterol conjugates: effect of the spacer arm between PEG and cholesterol on liposomal characteristics. Chem Phys Lipids 113(1\u20132):97\u2013110, S0009308401001785 [pii]","journal-title":"Chem Phys Lipids"},{"issue":"2","key":"41_CR29","doi-asserted-by":"publisher","first-page":"1586","DOI":"10.1016\/j.carbpol.2012.11.011","volume":"92","author":"M Muthiah","year":"2013","unstructured":"Muthiah M, Vu-Quang H, Kim YK, Rhee JH, Kang SH, Jun SY, Choi YJ, Jeong YY, Cho CS, Park IK (2013) Mannose-poly(ethylene glycol)-linked SPION targeted to antigen presenting cells for magnetic resonance imaging on lymph node. Carbohydr Polym 92(2):1586\u20131595. doi:10.1016\/j.carbpol.2012.11.011, S0144-8617(12)01116-2 [pii]","journal-title":"Carbohydr Polym"},{"key":"41_CR30","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1385\/1-59259-201-5:13","volume":"117","author":"JR Harris","year":"1999","unstructured":"Harris JR (1999) Negative staining of thinly spread biological particulates. Methods Mol Biol 117:13\u201330. doi:10.1385\/1-59259-201-5:13, 1-59259-201-5-13 [pii]","journal-title":"Methods Mol Biol"},{"key":"41_CR31","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","volume":"72","author":"MM Bradford","year":"1976","unstructured":"Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248\u2013254, S0003269776699996 [pii]","journal-title":"Anal Biochem"}],"container-title":["Methods in Molecular Biology","Vaccine Design"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-4939-3389-1_41","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,12,22]],"date-time":"2020-12-22T10:06:41Z","timestamp":1608631601000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-4939-3389-1_41"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"ISBN":["9781493933884","9781493933891"],"references-count":31,"URL":"https:\/\/doi.org\/10.1007\/978-1-4939-3389-1_41","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016]]},"assertion":[{"value":"14 April 2016","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}