{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,13]],"date-time":"2026-11-13T01:38:32Z","timestamp":1794533912636,"version":"4.9.1"},"publisher-location":"New York, NY","reference-count":27,"publisher":"Springer New York","isbn-type":[{"value":"9781493966028","type":"print"},{"value":"9781493966035","type":"electronic"}],"license":[{"start":{"date-parts":[[2016,11,5]],"date-time":"2016-11-05T00:00:00Z","timestamp":1478304000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017]]},"DOI":"10.1007\/978-1-4939-6603-5_6","type":"book-chapter","created":{"date-parts":[[2016,11,4]],"date-time":"2016-11-04T09:52:32Z","timestamp":1478253152000},"page":"97-105","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Synchronization of Mammalian Cell Cultures by Serum Deprivation"],"prefix":"10.1007","author":[{"given":"Thomas J.","family":"Langan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyla R.","family":"Rodgers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard C.","family":"Chou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,11,5]]},"reference":[{"key":"6_CR1","first-page":"983","volume-title":"Molecular biology of the cell","author":"B Alberts","year":"2002","unstructured":"Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P (2002) Molecular biology of the cell, 4th edn. Garland Science, New York, pp 983\u20131026","edition":"4"},{"key":"6_CR2","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/S0076-6879(79)58141-5","volume":"58","author":"T Ashihara","year":"1979","unstructured":"Ashihara T, Baserga R (1979) Cell synchronization. Methods Enzymol 58:248\u2013262","journal-title":"Methods Enzymol"},{"key":"6_CR3","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/j.tcb.2010.01.006","volume":"20","author":"P Salomoni","year":"2010","unstructured":"Salomoni P, Callegari F (2010) Cell cycle control of mammalian neural stem cells: putting a speed limit on G 1. Trends Cell Biol 20:233\u2013243","journal-title":"Trends Cell Biol"},{"issue":"2","key":"6_CR4","first-page":"471","volume":"29","author":"S Mueller","year":"2006","unstructured":"Mueller S, Schittenhelm M, Honecker F, Malenke E, Lauber K, Wesselborg S, Hartmann JT, Bokemeyer C, Mayer F (2006) Cell-cycle progression and response of germ cell tumors to cisplatin in vitro. Int J Oncol 29(2):471\u2013479","journal-title":"Int J Oncol"},{"key":"6_CR5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.pneurobio.2009.01.007","volume":"89","author":"W Wang","year":"2009","unstructured":"Wang W, Bu B, Zhang M, Yu Z, Tao D (2009) Neural cell cycle dysregulation and central nervous system diseases. Prog Neurobiol 89:1\u201317","journal-title":"Prog Neurobiol"},{"key":"6_CR6","doi-asserted-by":"publisher","first-page":"1286","DOI":"10.1073\/pnas.71.4.1286","volume":"71","author":"AB Pardee","year":"1974","unstructured":"Pardee AB (1974) A restriction point for control of normal animal cell proliferation. Proc Natl Acad Sci U S A 71:1286\u20131290","journal-title":"Proc Natl Acad Sci U S A"},{"key":"6_CR7","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1016\/0076-6879(95)54009-1","volume":"254","author":"W Krek","year":"1995","unstructured":"Krek W, DeCaprio JA (1995) Cell synchronization. Methods Enzym 254:114\u2013124","journal-title":"Methods Enzym"},{"key":"6_CR8","doi-asserted-by":"publisher","first-page":"1283","DOI":"10.1111\/j.1471-4159.1986.tb00651.x","volume":"46","author":"TJ Langan","year":"1986","unstructured":"Langan TJ, Volpe JJ (1986) Obligatory relationship between the sterol biosynthetic pathway and DNA synthesis and cell proliferation in glial primary cultures. J Neurochem 46:1283\u20131291","journal-title":"J Neurochem"},{"key":"6_CR9","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1016\/0165-3806(96)00109-5","volume":"96","author":"V Li","year":"1996","unstructured":"Li V, Kelly K, Schrot R, Langan TJ (1996) Cell cycle kinetics and commitment in newborn, adult, and tumoral astrocytes. Brain Res 96:138\u2013147","journal-title":"Brain Res"},{"key":"6_CR10","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/S0021-9258(18)33268-X","volume":"258","author":"V Quesney-Huneeus","year":"1983","unstructured":"Quesney-Huneeus V, Galick HA, Siperstein MD, Erickson SK, Spencer TA, Nelson JA (1983) The dual role of mevalonate in the cell cycle. J Biol Chem 258:378\u2013385","journal-title":"J Biol Chem"},{"key":"6_CR11","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1016\/0014-4827(77)90180-X","volume":"106","author":"BF Mitchell","year":"1977","unstructured":"Mitchell BF, Tupper JT (1977) Synchronization of mouse 3T3 and SV40 3T3 cells by way of centrifugal elutriation. Exp Cell Res 106:351\u2013355","journal-title":"Exp Cell Res"},{"key":"6_CR12","first-page":"3602","volume":"51","author":"K Keyomarsi","year":"1991","unstructured":"Keyomarsi K, Sandoval L, Band V, Pardee AB (1991) Synchronization of tumor and normal cells from G1 to multiple cell cycles by lovastatin. Cancer Res 51:3602\u20133609","journal-title":"Cancer Res"},{"key":"6_CR13","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1016\/0955-0674(92)90031-7","volume":"4","author":"AB Pardee","year":"1992","unstructured":"Pardee AB, Keyomarsi K (1992) Modification of cell proliferation with inhibitors. Curr Opin Cell Biol 4:186\u2013191","journal-title":"Curr Opin Cell Biol"},{"key":"6_CR14","doi-asserted-by":"publisher","first-page":"647","DOI":"10.1016\/0092-8674(81)90426-8","volume":"23","author":"JA Huberman","year":"1981","unstructured":"Huberman JA (1981) New views of the biochemistry of eucaryotic DNA replication revealed by aphidicolin, an unusual inhibitor of DNA polymerase alpha. Cell 23:647\u2013648","journal-title":"Cell"},{"key":"6_CR15","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/0006-8993(91)91099-M","volume":"548","author":"TJ Langan","year":"1991","unstructured":"Langan TJ, Slater MC (1991) Quiescent astroglia in long-term primary cultures re-enter the cell cycle and require a nonsterol isoprenoid in late G1. Brain Res 548:9\u201317","journal-title":"Brain Res"},{"key":"6_CR16","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1016\/0014-4827(80)90279-7","volume":"126","author":"GW Zieve","year":"1980","unstructured":"Zieve GW, Turnbull D, Mullins JM, McIntosh JR (1980) Production of large numbers of mitotic mammalian cells by use of the reversible microtubule inhibitor nocodazole. Exp Cell Res 126:397\u2013405","journal-title":"Exp Cell Res"},{"key":"6_CR17","doi-asserted-by":"publisher","first-page":"C927","DOI":"10.1152\/ajpcell.00006.2013","volume":"304","author":"L Henderson","year":"2013","unstructured":"Henderson L, Bortone DS, Lim C, Zambon AC (2013) Classic \u201cbroken cell\u201d techniques and newer live cell methods for cell cycle assessment. Am J Physiol Cell Physiol 304:C927\u2013C938","journal-title":"Am J Physiol Cell Physiol"},{"key":"6_CR18","first-page":"215","volume":"4","author":"I Sanchez","year":"1993","unstructured":"Sanchez I, Goya L, Vallerga AK, Firestone GL (1993) Glucocorticoids reversibly arrest rat hepatoma cell growth by inducing an early G1 block in cell cycle progression. Cell Growth Differ 4:215\u2013225","journal-title":"Cell Growth Differ"},{"key":"6_CR19","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1016\/0014-4827(63)90306-9","volume":"30","author":"T Terasima","year":"1963","unstructured":"Terasima T, Tolmach LJ (1963) Growth and nucleic acid synthesis in synchronously dividing populations of HELA cells. Exp Cell Res 30:344\u2013362","journal-title":"Exp Cell Res"},{"key":"6_CR20","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1016\/0165-4608(83)90044-4","volume":"8","author":"LM Webber","year":"1983","unstructured":"Webber LM, Garson OM (1983) Fluorodeoxyuridine synchronization of bone marrow cultures. Cancer Genet Cytogenet 8:123\u2013132","journal-title":"Cancer Genet Cytogenet"},{"key":"6_CR21","doi-asserted-by":"publisher","first-page":"2049","DOI":"10.1002\/jcb.25160","volume":"116","author":"TY Chen","year":"2015","unstructured":"Chen TY, Syu JS, Han TY, Cheng HL, Lu FI, Wang CY (2015) Cell cycle-dependent localization of dynactin subunit p150 glued at centrosome. J Cell Biochem 116:2049\u20132060","journal-title":"J Cell Biochem"},{"key":"6_CR22","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.ceb.2014.10.006","volume":"33","author":"IC Lopez-Mejia","year":"2015","unstructured":"Lopez-Mejia IC, Fajas L (2015) Cell cycle regulation of mitochondrial function. Curr Opin Cell Biol 33:19\u201325 [review]","journal-title":"Curr Opin Cell Biol"},{"key":"6_CR23","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1002\/jcp.1040890208","volume":"89","author":"JC Bartholomew","year":"1976","unstructured":"Bartholomew JC, Neff NT, Ross PA (1976) Stimulation of WI-38 cell cycle transit: effect of serum concentration and cell density. J Cell Physiol 89:251\u2013258","journal-title":"J Cell Physiol"},{"key":"6_CR24","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1128\/MMBR.21.4.263-272.1957","volume":"21","author":"A Campbell","year":"1957","unstructured":"Campbell A (1957) Synchronization of cell division. Bacteriol Rev 21:263\u2013272","journal-title":"Bacteriol Rev"},{"key":"6_CR25","doi-asserted-by":"publisher","first-page":"393","DOI":"10.1016\/0010-4809(78)90020-4","volume":"11","author":"DA Johnston","year":"1978","unstructured":"Johnston DA, White RA, Barlogie B (1978) Automatic processing and interpretation of DNA distributions: comparison of several techniques. Comput Biomed Res 11:393\u2013404","journal-title":"Comput Biomed Res"},{"key":"6_CR26","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/S0091-679X(08)61582-4","volume":"57","author":"GF Merrill","year":"1998","unstructured":"Merrill GF (1998) Cell synchronization. Methods Cell Biol 57:229\u2013249","journal-title":"Methods Cell Biol"},{"key":"6_CR27","doi-asserted-by":"publisher","first-page":"810","DOI":"10.1038\/385810a0","volume":"385","author":"I Wilmut","year":"1997","unstructured":"Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KHS (1997) Viable offspring derived from fetal and adult mammalian cells. Nature 385:810\u2013813","journal-title":"Nature"}],"container-title":["Methods in Molecular Biology","Cell Cycle Synchronization"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-4939-6603-5_6","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T08:27:59Z","timestamp":1657614479000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-4939-6603-5_6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,5]]},"ISBN":["9781493966028","9781493966035"],"references-count":27,"URL":"https:\/\/doi.org\/10.1007\/978-1-4939-6603-5_6","relation":{"is-part-of":[{"id-type":"doi","id":"https:\/\/doi.org\/10.1007\/978-1-4939-6603-5","asserted-by":"object"}]},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,5]]},"assertion":[{"value":"5 November 2016","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}