{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T21:11:37Z","timestamp":1793481097063,"version":"4.5.0"},"publisher-location":"New York, NY","reference-count":29,"publisher":"Springer New York","isbn-type":[{"value":"9781493969326","type":"print"},{"value":"9781493969340","type":"electronic"}],"license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017]]},"DOI":"10.1007\/978-1-4939-6934-0_11","type":"book-chapter","created":{"date-parts":[[2017,4,29]],"date-time":"2017-04-29T09:38:06Z","timestamp":1493458686000},"page":"179-189","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells"],"prefix":"10.1007","author":[{"given":"Ida","family":"Eriksson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"\u00d6llinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna","family":"Appelqvist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,4,30]]},"reference":[{"key":"11_CR1","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1146\/annurev-physiol-012110-142317","volume":"74","author":"JA Mindell","year":"2012","unstructured":"Mindell JA (2012) Lysosomal acidification mechanisms. Annu Rev Physiol 74:69\u201386. doi: 10.1146\/annurev-physiol-012110-142317","journal-title":"Annu Rev Physiol"},{"issue":"45","key":"11_CR2","doi-asserted-by":"publisher","first-page":"27360","DOI":"10.1074\/jbc.M115.659128","volume":"290","author":"LA Stransky","year":"2015","unstructured":"Stransky LA, Forgac M (2015) Amino acid availability modulates vacuolar H+-ATPase assembly. J Biol Chem 290(45):27360\u201327369. doi: 10.1074\/jbc.M115.659128","journal-title":"J Biol Chem"},{"issue":"21","key":"11_CR3","doi-asserted-by":"publisher","first-page":"7972","DOI":"10.1073\/pnas.85.21.7972","volume":"85","author":"EJ Bowman","year":"1988","unstructured":"Bowman EJ, Siebers A, Altendorf K (1988) Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells. Proc Natl Acad Sci U S A 85(21):7972\u20137976","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"7","key":"11_CR4","doi-asserted-by":"publisher","first-page":"3327","DOI":"10.1073\/pnas.75.7.3327","volume":"75","author":"S Ohkuma","year":"1978","unstructured":"Ohkuma S, Poole B (1978) Fluorescence probe measurement of the intralysosomal pH in living cells and the perturbation of pH by various agents. Proc Natl Acad Sci U S A 75(7):3327\u20133331","journal-title":"Proc Natl Acad Sci U S A"},{"key":"11_CR5","doi-asserted-by":"publisher","first-page":"7007","DOI":"10.1038\/ncomms8007","volume":"6","author":"C Mauvezin","year":"2015","unstructured":"Mauvezin C, Nagy P, Juhasz G, Neufeld TP (2015) Autophagosome-lysosome fusion is independent of V-ATPase-mediated acidification. Nat Commun 6:7007. doi: 10.1038\/ncomms8007","journal-title":"Nat Commun"},{"key":"11_CR6","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/0002-9394(72)90137-7","volume":"73","author":"MS Ramsey","year":"1972","unstructured":"Ramsey MS, Fine BS (1972) Chloroquine toxicity in the human eye. Histopathologic observations by electron microscopy. Am J Ophthalmol 73:229\u2013235","journal-title":"Am J Ophthalmol"},{"issue":"6","key":"11_CR7","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1034\/j.1600-0463.2002.100606.x","volume":"110","author":"SP Sundelin","year":"2002","unstructured":"Sundelin SP, Terman A (2002) Different effects of chloroquine and hydroxychloroquine on lysosomal function in cultured retinal pigment epithelial cells. APMIS 110(6):481\u2013489","journal-title":"APMIS"},{"issue":"4","key":"11_CR8","doi-asserted-by":"publisher","first-page":"1490","DOI":"10.1091\/mbc.E06-10-0975","volume":"18","author":"A Majumdar","year":"2007","unstructured":"Majumdar A, Cruz D, Asamoah N, Buxbaum A, Sohar I, Lobel P, Maxfield FR (2007) Activation of microglia acidifies lysosomes and leads to degradation of Alzheimer amyloid fibrils. Mol Biol Cell 18(4):1490\u20131496. doi: 10.1091\/mbc.E06-10-0975","journal-title":"Mol Biol Cell"},{"issue":"38","key":"11_CR9","doi-asserted-by":"publisher","first-page":"39982","DOI":"10.1074\/jbc.M406698200","volume":"279","author":"N Kokkonen","year":"2004","unstructured":"Kokkonen N, Rivinoja A, Kauppila A, Suokas M, Kellokumpu I, Kellokumpu S (2004) Defective acidification of intracellular organelles results in aberrant secretion of cathepsin D in cancer cells. J Biol Chem 279(38):39982\u201339988. doi: 10.1074\/jbc.M406698200","journal-title":"J Biol Chem"},{"issue":"9","key":"11_CR10","doi-asserted-by":"publisher","first-page":"1185","DOI":"10.1002\/hed.21317","volume":"32","author":"C Nilsson","year":"2010","unstructured":"Nilsson C, Roberg K, Grafstr\u00f6m RC, \u00d6llinger K (2010) Intrinsic differences in cisplatin sensitivity of head and neck cancer cell lines: correlation to lysosomal pH. Head Neck 32(9):1185\u20131194","journal-title":"Head Neck"},{"issue":"8","key":"11_CR11","doi-asserted-by":"publisher","first-page":"1327","DOI":"10.1038\/sj.bjc.6600861","volume":"88","author":"SS Chauhan","year":"2003","unstructured":"Chauhan SS, Liang XJ, Su AW, Pai-Panandiker A, Shen DW, Hanover JA, Gottesman MM (2003) Reduced endocytosis and altered lysosome function in cisplatin-resistant cell lines. Br J Cancer 88(8):1327\u20131334. doi: 10.1038\/sj.bjc.6600861","journal-title":"Br J Cancer"},{"issue":"10","key":"11_CR12","doi-asserted-by":"publisher","first-page":"1595","DOI":"10.1158\/1535-7163.MCT-05-0102","volume":"4","author":"R Safaei","year":"2005","unstructured":"Safaei R, Larson BJ, Cheng TC, Gibson MA, Otani S, Naerdemann W, Howell SB (2005) Abnormal lysosomal trafficking and enhanced exosomal export of cisplatin in drug-resistant human ovarian carcinoma cells. Mol Cancer Ther 4(10):1595\u20131604. doi: 10.1158\/1535-7163.MCT-05-0102","journal-title":"Mol Cancer Ther"},{"issue":"3","key":"11_CR13","doi-asserted-by":"publisher","first-page":"472","DOI":"10.1080\/15548627.2015.1136769","volume":"12","author":"M Bourdenx","year":"2016","unstructured":"Bourdenx M, Daniel J, Genin E, Soria FN, Blanchard-Desce M, Bezard E, Dehay B (2016) Nanoparticles restore lysosomal acidification defects: implications for Parkinson and other lysosomal-related diseases. Autophagy 12(3):472\u2013483. doi: 10.1080\/15548627.2015.1136769","journal-title":"Autophagy"},{"issue":"33","key":"11_CR14","doi-asserted-by":"publisher","first-page":"21783","DOI":"10.1074\/jbc.R109.023820","volume":"284","author":"B Turk","year":"2009","unstructured":"Turk B, Turk V (2009) Lysosomes as \"suicide bags\" in cell death: myth or reality? J Biol Chem 284(33):21783\u201321787. doi: 10.1074\/jbc.R109.023820","journal-title":"J Biol Chem"},{"key":"11_CR15","first-page":"128","volume-title":"Subcellular particles","author":"C Duve de","year":"1959","unstructured":"de Duve C (1959) Lysosomes, a new group of cytoplasmic particles. In: Hayashi T (ed) Subcellular particles. The Ronald Press Co., New York, pp 128\u2013159"},{"issue":"1","key":"11_CR16","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/S0014-5793(00)01286-2","volume":"470","author":"W Li","year":"2000","unstructured":"Li W, Yuan X, Nordgren G, Dalen H, Dubowchik GM, Firestone RA, Brunk UT (2000) Induction of cell death by the lysosomotropic detergent MSDH. FEBS Lett 470(1):35\u201339","journal-title":"FEBS Lett"},{"issue":"12","key":"11_CR17","doi-asserted-by":"crossref","first-page":"3950","DOI":"10.4049\/jimmunol.148.12.3950","volume":"148","author":"DL Thiele","year":"1992","unstructured":"Thiele DL, Lipsky PE (1992) Apoptosis is induced in cells with cytolytic potential by L-leucyl-L-leucine methyl ester. J Immunol 148(12):3950\u20133957","journal-title":"J Immunol"},{"issue":"5","key":"11_CR18","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1007\/s10495-009-0452-5","volume":"15","author":"AC Johansson","year":"2010","unstructured":"Johansson AC, Appelqvist H, Nilsson C, K\u00e5gedal K, Roberg K, \u00d6llinger K (2010) Regulation of apoptosis-associated lysosomal membrane permeabilization. Apoptosis 15(5):527\u2013540","journal-title":"Apoptosis"},{"issue":"9","key":"11_CR19","doi-asserted-by":"publisher","first-page":"1127","DOI":"10.1172\/JCI9914","volume":"106","author":"ME Guicciardi","year":"2000","unstructured":"Guicciardi ME, Deussing J, Miyoshi H, Bronk SF, Svingen PA, Peters C, Kaufmann SH, Gores GJ (2000) Cathepsin B contributes to TNF-alpha-mediated hepatocyte apoptosis by promoting mitochondrial release of cytochrome c. J Clin Invest 106(9):1127\u20131137","journal-title":"J Clin Invest"},{"issue":"5","key":"11_CR20","doi-asserted-by":"publisher","first-page":"1119","DOI":"10.1038\/sj.jid.5700124","volume":"126","author":"C Bivik","year":"2006","unstructured":"Bivik C, Larsson P, K\u00e5gedal K, Rosdahl I, \u00d6llinger K (2006) UVA\/B-induced apoptosis in human melanocytes involves translocation of cathepsins and Bcl-2 family members. J Invest Dermatol 126(5):1119\u20131127","journal-title":"J Invest Dermatol"},{"issue":"7","key":"11_CR21","doi-asserted-by":"publisher","first-page":"1149","DOI":"10.1007\/s10495-006-7108-5","volume":"11","author":"C Nilsson","year":"2006","unstructured":"Nilsson C, Johansson U, Johansson AC, K\u00e5gedal K, \u00d6llinger K (2006) Cytosolic acidification and lysosomal alkalinization during TNF-alpha induced apoptosis in U937 cells. Apoptosis 11(7):1149\u20131159","journal-title":"Apoptosis"},{"issue":"1","key":"11_CR22","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1007\/s11357-007-9045-9","volume":"30","author":"Y Stroikin","year":"2008","unstructured":"Stroikin Y, Mild H, Johansson U, Roberg K, Ollinger K (2008) Lysosome-targeted stress reveals increased stability of lipofuscin-containing lysosomes. Age (Dordr) 30(1):31\u201342. doi: 10.1007\/s11357-007-9045-9","journal-title":"Age (Dordr)"},{"issue":"16","key":"11_CR23","doi-asserted-by":"publisher","first-page":"2881","DOI":"10.1038\/sj.onc.1207512","volume":"23","author":"ME Guicciardi","year":"2004","unstructured":"Guicciardi ME, Leist M, Gores GJ (2004) Lysosomes in cell death. Oncogene 23(16):2881\u20132890","journal-title":"Oncogene"},{"issue":"1","key":"11_CR24","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/0014-4827(84)90698-0","volume":"150","author":"MJ Geisow","year":"1984","unstructured":"Geisow MJ (1984) Fluorescein conjugates as indicators of subcellular pH. A critical evaluation. Exp Cell Res 150(1):29\u201335","journal-title":"Exp Cell Res"},{"issue":"1","key":"11_CR25","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1006\/abio.1997.2466","volume":"255","author":"I Vergne","year":"1998","unstructured":"Vergne I, Constant P, Lan\u00e9elle G (1998) Phagosomal pH determination by dual fluorescence flow cytometry. Anal Biolchem 255(1):127\u2013132","journal-title":"Anal Biolchem"},{"issue":"1","key":"11_CR26","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1016\/0014-5793(75)81072-6","volume":"54","author":"D Reijngoud","year":"1975","unstructured":"Reijngoud D, Tager JM (1975) Effect of ionophores and temperature on intralysosomal pH. FEBS Lett 54(1):76\u201379","journal-title":"FEBS Lett"},{"issue":"5","key":"11_CR27","first-page":"1519","volume":"22","author":"BM Myers","year":"1995","unstructured":"Myers BM, Tietz PS, Tarara JE, LaRusso NF (1995) Dynamic measurements of the acute and chronic effects of lysosomotropic agents on hepatocyte lysosomal pH using flow cytometry. Hepatology 22(5):1519\u20131526","journal-title":"Hepatology"},{"issue":"3","key":"11_CR28","first-page":"231","volume":"42","author":"H Appelqvist","year":"2012","unstructured":"Appelqvist H, Johansson AC, Linderoth E, Johansson U, Antonsson B, Steinfeld R, K\u00e5gedal K, \u00d6llinger K (2012) Lysosome-mediated apoptosis is associated with cathepsin D-specific processing of Bid at Phe24, Trp48, and Phe183. Ann Clin Lab Sci 42(3):231\u2013242","journal-title":"Ann Clin Lab Sci"},{"issue":"1","key":"11_CR29","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1016\/j.yexcr.2012.10.004","volume":"319","author":"I Eriksson","year":"2013","unstructured":"Eriksson I, Joosten M, Roberg K, Ollinger K (2013) The histone deacetylase inhibitor trichostatin A reduces lysosomal pH and enhances cisplatin-induced apoptosis. Exp Cell Res 319(1):12\u201320. doi: 10.1016\/j.yexcr.2012.10.004","journal-title":"Exp Cell Res"}],"container-title":["Methods in Molecular Biology","Lysosomes"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-4939-6934-0_11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T10:21:25Z","timestamp":1692786085000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-4939-6934-0_11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"ISBN":["9781493969326","9781493969340"],"references-count":29,"URL":"https:\/\/doi.org\/10.1007\/978-1-4939-6934-0_11","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017]]},"assertion":[{"value":"30 April 2017","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}