{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,13]],"date-time":"2026-11-13T06:50:20Z","timestamp":1794552620901,"version":"4.9.1"},"publisher-location":"New York, NY","reference-count":38,"publisher":"Springer New York","isbn-type":[{"value":"9781493973859","type":"print"},{"value":"9781493973866","type":"electronic"}],"license":[{"start":{"date-parts":[[2017,11,19]],"date-time":"2017-11-19T00:00:00Z","timestamp":1511049600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018]]},"DOI":"10.1007\/978-1-4939-7386-6_20","type":"book-chapter","created":{"date-parts":[[2017,11,18]],"date-time":"2017-11-18T01:53:40Z","timestamp":1510970020000},"page":"423-444","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Quantitative Determination of Interacting Protein Surfaces in Prokaryotes and Eukaryotes by Using In-Cell NMR Spectroscopy"],"prefix":"10.1007","author":[{"given":"David S.","family":"Burz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher M.","family":"DeMott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asma","family":"Aldousary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Dansereau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Shekhtman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,11,19]]},"reference":[{"issue":"6817","key":"20_CR1","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1038\/35051615","volume":"409","author":"JC Rain","year":"2001","unstructured":"Rain JC, Selig L, De Reuse H, Battaglia V, Reverdy C, Simon S, Lenzen G, Petel F, Wojcik J, Schachter V, Chemama Y, Labigne A, Legrain P (2001) The protein-protein interaction map of helicobacter pylori. Nature 409(6817):211\u2013215","journal-title":"Nature"},{"issue":"6928","key":"20_CR2","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1038\/nature01513","volume":"422","author":"A Sali","year":"2003","unstructured":"Sali A, Glaeser R, Earnest T, Baumeister W (2003) From words to literature in structural proteomics. Nature 422(6928):216\u2013225","journal-title":"Nature"},{"issue":"5553","key":"20_CR3","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1126\/science.1068664","volume":"295","author":"M Gerstein","year":"2002","unstructured":"Gerstein M, Lan N, Jansen R (2002) Proteomics. Integrating interactomes. Science 295(5553):284\u2013287","journal-title":"Science"},{"issue":"10","key":"20_CR4","doi-asserted-by":"publisher","first-page":"2446","DOI":"10.1021\/ja0057528","volume":"123","author":"Z Serber","year":"2001","unstructured":"Serber Z, Keatinge-Clay AT, Ledwidge R, Kelly AE, Miller SM, Dotsch V (2001) High-resolution macromolecular NMR spectroscopy inside living cells. J Am Chem Soc 123(10):2446\u20132447","journal-title":"J Am Chem Soc"},{"key":"20_CR5","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-84925-6","volume-title":"The machinery of life","author":"D Goodsell","year":"2009","unstructured":"Goodsell D (2009) The machinery of life. Springer, New York"},{"key":"20_CR6","doi-asserted-by":"crossref","unstructured":"Persson E, Halle B (2008) Cell water dynamics on multiple time scales. Proc Natl Acad Sci USA 105(17):6266\u20136271","DOI":"10.1073\/pnas.0709585105"},{"key":"20_CR7","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4612-1228-7","volume-title":"Physics: with illustrative examples from medicine and biology:electricity and magnetism","author":"GB Benedek","year":"2000","unstructured":"Benedek GB, Villars FMH (2000) Physics: with illustrative examples from medicine and biology:electricity and magnetism. Springer-Verlag, New York"},{"issue":"3","key":"20_CR8","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1016\/j.pnmrs.2010.11.002","volume":"59","author":"AY Maldonado","year":"2011","unstructured":"Maldonado AY, Burz DS, Shekhtman A (2011) In-cell NMR spectroscopy. Prog Nucl Magn Reson Spectrosc 59(3):197\u2013212","journal-title":"Prog Nucl Magn Reson Spectrosc"},{"key":"20_CR9","doi-asserted-by":"crossref","unstructured":"Li C, Wang GF, Wang Y, Creager-Allen R, Lutz EA, Scronce H, Slade KM, Ruf RA, Mehl RA, Pielak GJ (2010) Protein 19F NMR in Escherichia coli. J Am Chem Soc 132(1):321\u2013327","DOI":"10.1021\/ja907966n"},{"key":"20_CR10","doi-asserted-by":"crossref","unstructured":"Crowley PB, Chow E, Papkovskaia T (2011) Protein interactions in the Escherichia coli cytosol: an impediment to in-cell NMR spectroscopy. ChemBioChem 12(7):1043\u20131048","DOI":"10.1002\/cbic.201100063"},{"issue":"17","key":"20_CR11","doi-asserted-by":"publisher","first-page":"2727","DOI":"10.1021\/acs.biochem.5b00036","volume":"54","author":"S Majumder","year":"2015","unstructured":"Majumder S, Xue J, DeMott CM, Reverdatto S, Burz DS, Shekhtman A (2015) Probing protein quinary interactions by in-cell nuclear magnetic resonance spectroscopy. Biochemistry 54(17):2727\u20132738","journal-title":"Biochemistry"},{"key":"20_CR12","doi-asserted-by":"publisher","first-page":"14456","DOI":"10.1038\/srep14456","volume":"5","author":"L Barbieri","year":"2015","unstructured":"Barbieri L, Luchinat E, Banci L (2015) Protein interaction patterns in different cellular environments are revealed by in-cell NMR. Sci Rep 5:14456","journal-title":"Sci Rep"},{"key":"20_CR13","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1002\/pro.2615","volume":"24","author":"C Kyne","year":"2015","unstructured":"Kyne C, Ruhle B, Gautier VW, Crowley PB (2015) Specific ion effects on macromolecular interactions in Escherichia coli extracts. Protein Sci 24:310\u2013318","journal-title":"Protein Sci"},{"key":"20_CR14","doi-asserted-by":"crossref","unstructured":"McConkey EH (1982) Molecular evolution, intracellular organization, and the quinary structure of proteins. Proc Natl Acad Sci USA 79(10):3236\u20133240","DOI":"10.1073\/pnas.79.10.3236"},{"issue":"11","key":"20_CR15","doi-asserted-by":"publisher","first-page":"984","DOI":"10.1002\/bies.201300080","volume":"35","author":"AJ Wirth","year":"2013","unstructured":"Wirth AJ, Gruebele M (2013) Quinary protein structure and the consequences of crowding in living cells: leaving the test-tube behind. Bioessays 35(11):984\u2013993","journal-title":"Bioessays"},{"key":"20_CR16","doi-asserted-by":"crossref","unstructured":"Sarkar M, Smith AE, Pielak GJ (2013) Impact of reconstituted cytosol on protein stability. Proc Natl Acad Sci USA 110(48):19342\u201319347","DOI":"10.1073\/pnas.1312678110"},{"key":"20_CR17","volume-title":"Protein NMR spectroscopy","author":"J Cavanagh","year":"2007","unstructured":"Cavanagh J, Fairbrother WJ, Palmer AG, Rance M, Skelton NJ (2007) Protein NMR spectroscopy. Academic Press, New York"},{"key":"20_CR18","doi-asserted-by":"crossref","unstructured":"Wider G (2005) NMR techniques used with very large biological macromolecules in solution. Meth Enzymol 394:382\u2013398","DOI":"10.1016\/S0076-6879(05)94015-9"},{"key":"20_CR19","doi-asserted-by":"crossref","unstructured":"Riek R, Wider G, Pervushin K, Wuthrich K (1999) Polarization transfer by cross-correlated relaxation in solution NMR with very large molecules. Proc Natl Acad Sci USA 96(9):4918\u20134923","DOI":"10.1073\/pnas.96.9.4918"},{"issue":"1-3","key":"20_CR20","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1016\/S0014-5793(02)03051-X","volume":"524","author":"A Shekhtman","year":"2002","unstructured":"Shekhtman A, Ghose R, Goger M, Cowburn D (2002) NMR structure determination and investigation using a reduced proton (REDPRO) labeling strategy for proteins. FEBS Lett 524(1-3):177\u2013182","journal-title":"FEBS Lett"},{"issue":"1","key":"20_CR21","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1007\/s10858-010-9409-9","volume":"47","author":"F Ferrage","year":"2010","unstructured":"Ferrage F, Dutta K, Shekhtman A, Cowburn D (2010) Structural determination of biomolecular interfaces by nuclear magnetic resonance of proteins with reduced proton density. J Biomol NMR 47(1):41\u201354","journal-title":"J Biomol NMR"},{"issue":"48","key":"20_CR22","doi-asserted-by":"publisher","first-page":"14317","DOI":"10.1021\/bi011751w","volume":"40","author":"Z Serber","year":"2001","unstructured":"Serber Z, Dotsch V (2001) In-cell NMR spectroscopy. Biochemistry 40(48):14317\u201314323","journal-title":"Biochemistry"},{"issue":"5","key":"20_CR23","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1038\/nchembio.1202","volume":"9","author":"L Banci","year":"2013","unstructured":"Banci L, Barbieri L, Bertini I, Luchinat E, Secci E, Zhao Y, Aricescu AR (2013) Atomic-resolution monitoring of protein maturation in live human cells by NMR. Nat Chem Biol 9(5):297\u2013299","journal-title":"Nat Chem Biol"},{"issue":"30","key":"20_CR24","doi-asserted-by":"publisher","first-page":"12798","DOI":"10.1021\/ja304809s","volume":"134","author":"K Bertrand","year":"2012","unstructured":"Bertrand K, Reverdatto S, Burz DS, Zitomer R, Shekhtman A (2012) Structure of proteins in eukaryotic compartments. J Am Chem Soc 134(30):12798\u201312806","journal-title":"J Am Chem Soc"},{"issue":"5","key":"20_CR25","doi-asserted-by":"publisher","first-page":"1688","DOI":"10.1021\/ja310928u","volume":"135","author":"J Hamatsu","year":"2013","unstructured":"Hamatsu J, O'Donovan D, Tanaka T, Shirai T, Hourai Y, Mikawa T, Ikeya T, Mishima M, Boucher W, Smith BO, Laue ED, Shirakawa M, Ito Y (2013) High-resolution heteronuclear multidimensional NMR of proteins in living insect cells using a baculovirus protein expression system. J Am Chem Soc 135(5):1688\u20131691","journal-title":"J Am Chem Soc"},{"issue":"7234","key":"20_CR26","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1038\/nature07839","volume":"458","author":"K Inomata","year":"2009","unstructured":"Inomata K, Ohno A, Tochio H, Isogai S, Tenno T, Nakase I, Takeuchi T, Futaki S, Ito Y, Hiroaki H, Shirakawa M (2009) High-resolution multi-dimensional NMR spectroscopy of proteins in human cells. Nature 458(7234):106\u2013109","journal-title":"Nature"},{"key":"20_CR27","doi-asserted-by":"crossref","unstructured":"Ogino S, Kubo S, Umemoto R, Huang S, Nishida N, Shimada I (2009) Observation of NMR signals from proteins introduced into living mammalian cells by reversible membrane permeabilization using a pore-formingtoxin, Streptolysin O. J Am Chem Soc 131(31):10834\u201310835","DOI":"10.1021\/ja904407w"},{"key":"20_CR28","doi-asserted-by":"crossref","unstructured":"Selenko P, Serber Z, Gadea B, Ruderman J, Wagner G (2006) Quantitative NMR analysis of the protein G B1 domain in Xenopus laevis egg extracts and intact oocytes. Proc Natl Acad Sci USA 103(32):11904\u201311909","DOI":"10.1073\/pnas.0604667103"},{"issue":"3","key":"20_CR29","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1007\/s10858-006-9079-9","volume":"36","author":"T Sakai","year":"2006","unstructured":"Sakai T, Tochio H, Tenno T, Ito Y, Kokubo T, Hiroaki H, Shirakawa M (2006) In-cell NMR spectroscopy of proteins inside Xenopus laevis oocytes. J Biomol NMR 36(3):179\u2013188","journal-title":"J Biomol NMR"},{"issue":"7588","key":"20_CR30","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1038\/nature16531","volume":"530","author":"FX Theillet","year":"2016","unstructured":"Theillet FX, Binolfi A, Bekei B, Martorana A, Rose HM, Stuiver M, Verzini S, Lorenz D, van Rossum M, Goldfarb D, Selenko P (2016) Structural disorder of monomeric alpha-synuclein persists in mammalian cells. Nature 530(7588):45\u201350","journal-title":"Nature"},{"issue":"32","key":"20_CR31","doi-asserted-by":"publisher","first-page":"4568","DOI":"10.1021\/acs.biochem.6b00330","volume":"55","author":"S Majumder","year":"2016","unstructured":"Majumder S, DeMott CM, Reverdatto S, Burz DS, Shekhtman A (2016) Total cellular RNA modulates protein activity. Biochemistry 55(32):4568\u20134573","journal-title":"Biochemistry"},{"issue":"2","key":"20_CR32","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1038\/nmeth851","volume":"3","author":"DS Burz","year":"2006","unstructured":"Burz DS, Dutta K, Cowburn D, Shekhtman A (2006) Mapping structural interactions using in-cell NMR spectroscopy (STINT-NMR). Nat Methods 3(2):91\u201393","journal-title":"Nat Methods"},{"key":"20_CR33","unstructured":"Burz DS, Shekhtman A (2010) The STINT-NMR method for studying in-cell protein-protein interactions. Curr Protoc Protein Sci. Chapter 17:Unit 17 11"},{"issue":"1","key":"20_CR34","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1038\/nprot.2006.23","volume":"1","author":"DS Burz","year":"2006","unstructured":"Burz DS, Dutta K, Cowburn D, Shekhtman A (2006) In-cell NMR for protein-protein interactions (STINT-NMR). Nat Protoc 1(1):146\u2013152","journal-title":"Nat Protoc"},{"key":"20_CR35","volume-title":"Matrix computations","author":"GH Golub","year":"2012","unstructured":"Golub GH, Van Loan CF (2012) Matrix computations, 4th edn. The Johns Hopkins University Press, Baltimore, USA","edition":"4"},{"issue":"9","key":"20_CR36","doi-asserted-by":"publisher","first-page":"e74576","DOI":"10.1371\/journal.pone.0074576","volume":"8","author":"AY Maldonado","year":"2013","unstructured":"Maldonado AY, Burz DS, Reverdatto S, Shekhtman A (2013) Fate of pup inside the mycobacterium proteasome studied by in-cell NMR. PLoS One 8(9):e74576","journal-title":"PLoS One"},{"key":"20_CR37","doi-asserted-by":"crossref","unstructured":"Majumder S, DeMott CM, Burz DS, Shekhtman A (2014) Using singular value decomposition to characterizeprotein-protein interactions by in-cell NMR spectroscopy. ChemBioChem 15(7):929\u2013933","DOI":"10.1002\/cbic.201400030"},{"issue":"11","key":"20_CR38","doi-asserted-by":"publisher","first-page":"1352","DOI":"10.1038\/nsmb.1918","volume":"17","author":"T Wang","year":"2010","unstructured":"Wang T, Darwin KH, Li H (2010) Binding-induced folding of prokaryotic ubiquitin-like protein on the mycobacterium proteasomal ATPase targets substrates for degradation. Nat Struct Mol Biol 17(11):1352\u20131357","journal-title":"Nat Struct Mol Biol"}],"container-title":["Methods in Molecular Biology","Protein NMR"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-4939-7386-6_20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,17]],"date-time":"2019-05-17T03:52:05Z","timestamp":1558065125000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-4939-7386-6_20"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,19]]},"ISBN":["9781493973859","9781493973866"],"references-count":38,"URL":"https:\/\/doi.org\/10.1007\/978-1-4939-7386-6_20","relation":{"is-part-of":[{"id-type":"doi","id":"https:\/\/doi.org\/10.1007\/978-1-4939-7386-6","asserted-by":"object"}]},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,19]]},"assertion":[{"value":"19 November 2017","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}