{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T12:29:59Z","timestamp":1775824199604,"version":"3.50.1"},"publisher-location":"New York, NY","reference-count":31,"publisher":"Springer New York","isbn-type":[{"value":"9781493974733","type":"print"},{"value":"9781493974740","type":"electronic"}],"license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018]]},"DOI":"10.1007\/978-1-4939-7474-0_14","type":"book-chapter","created":{"date-parts":[[2018,1,31]],"date-time":"2018-01-31T11:33:18Z","timestamp":1517398398000},"page":"193-205","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The Use of Intravital Two-Photon and Thick Section Confocal Imaging to Analyze B Lymphocyte Trafficking in Lymph Nodes and Spleen"],"prefix":"10.1007","author":[{"given":"Chung","family":"Park","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Il-Young","family":"Hwang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John H.","family":"Kehrl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2018,2,1]]},"reference":[{"issue":"8","key":"14_CR1","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1038\/nri1669","volume":"5","author":"RE Mebius","year":"2005","unstructured":"Mebius RE, Kraal G (2005) Structure and function of the spleen. Nat Rev Immunol 5(8):606\u2013616","journal-title":"Nat Rev Immunol"},{"key":"14_CR2","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1126\/science.272.5258.60","volume":"272","author":"EC Butcher","year":"1996","unstructured":"Butcher EC, Picker LJ (1996) Lymphocyte homing and homeostasis. Science 272:60\u201366","journal-title":"Science"},{"key":"14_CR3","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1146\/annurev.immunol.21.090501.080131","volume":"22","author":"SD Rosen","year":"2004","unstructured":"Rosen SD (2004) Ligands for L-selectin: homing, inflammation, and beyond. Annu Rev Immunol 22:129\u2013156","journal-title":"Annu Rev Immunol"},{"issue":"2","key":"14_CR4","first-page":"469","volume":"152","author":"EL Berg","year":"1998","unstructured":"Berg EL, Mullowney AT, Andrew DP, Goldberg JE, Butcher EC (1998) Complexity and differential expression of carbohydrate epitopes associated with L-selectin recognition of high endothelial venules. Am J Pathol Feb 152(2):469\u2013477","journal-title":"Am J Pathol Feb"},{"issue":"6456","key":"14_CR5","doi-asserted-by":"publisher","first-page":"695","DOI":"10.1038\/366695a0","volume":"366","author":"EL Berg","year":"1993","unstructured":"Berg EL, McEvoy LM, Berlin C, Bargatze RF, Butcher EC (1993) L-selectin-mediated lymphocyte rolling on MAdCAM-1. Nature 366(6456):695\u2013698","journal-title":"Nature"},{"issue":"3","key":"14_CR6","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.4049\/jimmunol.157.3.1096","volume":"157","author":"DA Steeber","year":"1996","unstructured":"Steeber DA, Green NE, Sato S, Tedder TF (1996) Lyphocyte migration in L-selectin-deficient mice. Altered subset migration and aging of the immune system. J Immunol 157(3):1096\u20131106","journal-title":"J Immunol"},{"issue":"5921","key":"14_CR7","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1038\/304030a0","volume":"304","author":"WM Gallatin","year":"1983","unstructured":"Gallatin WM, Weissman IL, Butcher EC (1983) A cell-surface molecule involved in organ-specific homing of lymphocytes. Nature 304(5921):30\u201334","journal-title":"Nature"},{"issue":"5392","key":"14_CR8","doi-asserted-by":"publisher","first-page":"1281","DOI":"10.1126\/science.282.5392.1281","volume":"282","author":"JJ Peschon","year":"1998","unstructured":"Peschon JJ, Slack JL, Reddy P, Stocking KL, Sunnarborg SW, Lee DC, Russell WE, Castner BJ, Johnson RS, Fitzner JN, Boyce RW, Nelson N, Kozlosky CJ, Wolfson MF, Rauch CT, Cerretti DP, Paxton RJ, March CJ, Black RA (1998) An essential role for ectodomain shedding in mammalian development. Science 282(5392):1281\u20131284","journal-title":"Science"},{"key":"14_CR9","doi-asserted-by":"publisher","first-page":"384","DOI":"10.1016\/j.immuni.2008.12.020","volume":"30","author":"Z Shulman","year":"2009","unstructured":"Shulman Z, Shinder V, Klein E et al (2009) Lymphocyte crawling and transendothelial migration require chemokine triggering of high-affinity LFA-1 integrin. Immunity 30:384\u2013396","journal-title":"Immunity"},{"key":"14_CR10","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1111\/j.1600-065X.1997.tb00955.x","volume":"156","author":"JE Gretz","year":"1997","unstructured":"Gretz JE, Anderson AO, Shaw S (1997) Cords, channels, corridors and conduits: critical architectural elements facilitating cell interactions in the lymph node cortex. Immunol Rev 156:11\u201324","journal-title":"Immunol Rev"},{"key":"14_CR11","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1080\/01926230600867727","volume":"34","author":"CL Willard-Mack","year":"2006","unstructured":"Willard-Mack CL (2006) Normal structure, function, and histology of LNs. Toxicol Pathol 34:409\u2013424","journal-title":"Toxicol Pathol"},{"key":"14_CR12","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/j.acthis.2006.11.002","volume":"109","author":"B Ma","year":"2007","unstructured":"Ma B, Jablonska J, Lindenmaier W, Dittmar KE (2007) Immunohistochemical study of the reticular and vascular network of mouse lymph node using vibratome sections. Acta Histochem 109:15\u201328","journal-title":"Acta Histochem"},{"key":"14_CR13","doi-asserted-by":"publisher","first-page":"989","DOI":"10.1016\/j.immuni.2006.10.011","volume":"25","author":"M Baj\u00e9noff","year":"2006","unstructured":"Baj\u00e9noff M, Egen JG, Koo LY et al (2006) Stromal cell networks regulate lymphocyte entry, migration, and territoriality in lymph nodes. Immunity 25:989\u20131001","journal-title":"Immunity"},{"key":"14_CR14","doi-asserted-by":"publisher","first-page":"1037","DOI":"10.1016\/S0092-8674(00)81798-5","volume":"87","author":"R F\u00f6rster","year":"1996","unstructured":"F\u00f6rster R, Mattis AE, Kremmer E et al (1996) A putative chemokine receptor, BLR1, directs B cell migration to defined lymphoid organs and specific anatomic compartments of the spleen. Cell 87:1037\u20131047","journal-title":"Cell"},{"key":"14_CR15","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1038\/416094a","volume":"416","author":"K Reif","year":"2002","unstructured":"Reif K, Ekland EH, Ohl L et al (2002) Balanced responsiveness to chemoattractants from adjacent zones determines B-cell position. Nature 416:94\u201399","journal-title":"Nature"},{"key":"14_CR16","doi-asserted-by":"publisher","first-page":"978","DOI":"10.1182\/blood-2011-06-364273","volume":"119","author":"C Park","year":"2012","unstructured":"Park C, Hwang IY, Sinha RK et al (2012) Lymph node B lymphocyte trafficking is constrained by anatomy and highly dependent upon chemoattractant desensitization. Blood 119:978\u2013989","journal-title":"Blood"},{"key":"14_CR17","doi-asserted-by":"publisher","first-page":"434","DOI":"10.1016\/j.immuni.2008.12.018","volume":"30","author":"RK Sinha","year":"2009","unstructured":"Sinha RK, Park C, Hwang IY et al (2009) B lymphocytes exit lymph nodes through cortical lymphatic sinusoids by a mechanism independent of sphingosine-1-phosphate-mediated chemotaxis. Immunity 30:434\u2013446","journal-title":"Immunity"},{"key":"14_CR18","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1146\/annurev.bioeng.2.1.399","volume":"2","author":"PT So","year":"2000","unstructured":"So PT, Dong CY, Masters BR, Berland KM (2000) Two-photon excitation fluorescence microscopy. Annu Rev Biomed Eng 2:399\u2013429","journal-title":"Annu Rev Biomed Eng"},{"key":"14_CR19","doi-asserted-by":"publisher","first-page":"1869","DOI":"10.1126\/science.1070051","volume":"296","author":"MJ Miller","year":"2002","unstructured":"Miller MJ, Wei SH, Parker I, Cahalan MD (2002) Two-photon imaging of lymphocyte motility and antigen response in intact lymph node. Science 296:1869\u20131873","journal-title":"Science"},{"key":"14_CR20","doi-asserted-by":"publisher","first-page":"2604","DOI":"10.1073\/pnas.2628040100","volume":"100","author":"MJ Miller","year":"2003","unstructured":"Miller MJ, Wei SH, Cahalan MD, Parker I (2003) Autonomous T cell trafficking examined in vivo with intravital two-photon microscopy. Proc Natl Acad Sci U S A 100:2604\u20132609","journal-title":"Proc Natl Acad Sci U S A"},{"key":"14_CR21","doi-asserted-by":"publisher","first-page":"867","DOI":"10.1038\/nri1222","volume":"3","author":"UH Andrian von","year":"2003","unstructured":"von Andrian UH, Mempel TR (2003) Homing and cellular traffic in lymph nodes. Nat Rev Immunol 3:867\u2013878","journal-title":"Nat Rev Immunol"},{"key":"14_CR22","doi-asserted-by":"publisher","first-page":"287","DOI":"10.3109\/10739689609148303","volume":"3","author":"UH Andrian von","year":"1996","unstructured":"von Andrian UH (1996) Intravital microscopy of the peripheral lymph node microcirculation in mice. Microcirculation 3:287\u2013300","journal-title":"Microcirculation"},{"key":"14_CR23","doi-asserted-by":"publisher","first-page":"1572","DOI":"10.1182\/blood-2009-08-237917","volume":"115","author":"EJ Park","year":"2010","unstructured":"Park EJ, Peixoto A, Imai Y et al (2010) Distinct roles for LFA-1 affinity regulation during T-cell adhesion, diapedesis, and interstitial migration in lymph nodes. Blood 115:1572\u20131581","journal-title":"Blood"},{"key":"14_CR24","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1182\/blood-2009-11-254334","volume":"116","author":"RT Boscacci","year":"2010","unstructured":"Boscacci RT, Pfeiffer F, Gollmer K et al (2010) Comprehensive analysis of lymph node stroma-expressed Ig superfamily members reveals redundant and nonredundant roles for ICAM-1, ICAM-2, and VCAM-1 in lymphocyte homing. Blood 116:915\u2013925","journal-title":"Blood"},{"key":"14_CR25","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1016\/j.immuni.2005.01.017","volume":"22","author":"SB Han","year":"2005","unstructured":"Han SB, Moratz C, Huang NN et al (2005) Rgs1 and Gnai2 regulate the entrance of B lymphocytes into lymph nodes and B cell motility within lymph node follicles. Immunity 22:343\u2013354","journal-title":"Immunity"},{"key":"14_CR26","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1007\/978-1-60761-198-1_13","volume":"571","author":"C Park","year":"2009","unstructured":"Park C, Hwang IY, Kehrl JH (2009) Intravital two-photon imaging of adoptively transferred B lymphocytes in inguinal lymph nodes. Methods Mol Biol 571:199\u2013207","journal-title":"Methods Mol Biol"},{"key":"14_CR27","doi-asserted-by":"publisher","first-page":"e06467","DOI":"10.7554\/eLife.06467","volume":"4","author":"C Park","year":"2015","unstructured":"Park C, Arthos J, Cicala C, Kehrl JH (2015) The HIV-1 envelope protein gp120 is captured and displayed for B cell recognition by SIGN-R1(+) lymph node macrophages. eLife 4:e06467. https:\/\/doi.org\/10.7554\/eLife.06467","journal-title":"eLife"},{"key":"14_CR28","first-page":"161","volume":"271","author":"C Moratz","year":"2004","unstructured":"Moratz C, Kehrl JH (2004) In vitro and in vivo assays of B-lymphocyte migration. Methods Mol Biol 271:161\u2013171","journal-title":"Methods Mol Biol"},{"key":"14_CR29","unstructured":"of Thermo Fisher Scientific Inc. see https:\/\/www.thermofisher.com\/order\/catalog\/product\/11206D?SID=srch-srp-11206D ."},{"key":"14_CR30","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1038\/nature02238","volume":"427","author":"TR Mempel","year":"2004","unstructured":"Mempel TR, Henrickson SE, Von Andrian UH (2004) T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases. Nature 427:154\u2013159","journal-title":"Nature"},{"key":"14_CR31","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1038\/ni1210","volume":"6","author":"G Shakhar","year":"2005","unstructured":"Shakhar G, Lindquist RL, Skokos D et al (2005) Stable T cell-dendritic cell interactions precede the development of both tolerance and immunity in vivo. Nat Immunol 6:707\u2013714","journal-title":"Nat Immunol"}],"container-title":["Methods in Molecular Biology","B Cell Receptor Signaling"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-4939-7474-0_14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T19:40:19Z","timestamp":1693510819000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-4939-7474-0_14"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"ISBN":["9781493974733","9781493974740"],"references-count":31,"URL":"https:\/\/doi.org\/10.1007\/978-1-4939-7474-0_14","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018]]},"assertion":[{"value":"1 February 2018","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}