{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T20:17:00Z","timestamp":1793305020123,"version":"4.5.0"},"publisher-location":"New York, NY","reference-count":30,"publisher":"Springer New York","isbn-type":[{"value":"9781493986682","type":"print"},{"value":"9781493986699","type":"electronic"}],"license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019]]},"DOI":"10.1007\/978-1-4939-8669-9_11","type":"book-chapter","created":{"date-parts":[[2018,10,15]],"date-time":"2018-10-15T11:06:11Z","timestamp":1539601571000},"page":"153-164","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Fate Mapping In Vivo to Distinguish Bona Fide Microglia Versus Recruited Monocyte-Derived Macrophages in Retinal Disease"],"prefix":"10.1007","author":[{"given":"Nancy J.","family":"Reyes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rose","family":"Mathew","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel R.","family":"Saban","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","reference":[{"issue":"9","key":"11_CR1","doi-asserted-by":"publisher","first-page":"1142","DOI":"10.1038\/nn.2887","volume":"14","author":"B Ajami","year":"2011","unstructured":"Ajami B, Bennett JL, Krieger C, McNagny KM, Rossi FM (2011) Infiltrating monocytes trigger EAE progression, but do not contribute to the resident microglia pool. Nat Neurosci 14(9):1142\u20131149. https:\/\/doi.org\/10.1038\/nn.2887","journal-title":"Nat Neurosci"},{"issue":"12","key":"11_CR2","doi-asserted-by":"publisher","first-page":"1538","DOI":"10.1038\/nn2014","volume":"10","author":"B Ajami","year":"2007","unstructured":"Ajami B, Bennett JL, Krieger C, Tetzlaff W, Rossi FM (2007) Local self-renewal can sustain CNS microglia maintenance and function throughout adult life. Nat Neurosci 10(12):1538\u20131543. https:\/\/doi.org\/10.1038\/nn2014","journal-title":"Nat Neurosci"},{"issue":"6005","key":"11_CR3","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1126\/science.1194637","volume":"330","author":"F Ginhoux","year":"2010","unstructured":"Ginhoux F, Greter M, Leboeuf M, Nandi S, See P, Gokhan S, Mehler MF, Conway SJ, Ng LG, Stanley ER, Samokhvalov IM, Merad M (2010) Fate mapping analysis reveals that adult microglia derive from primitive macrophages. Science 330(6005):841\u2013845. https:\/\/doi.org\/10.1126\/science.1194637","journal-title":"Science"},{"issue":"8","key":"11_CR4","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a020537","volume":"7","author":"F Ginhoux","year":"2015","unstructured":"Ginhoux F, Prinz M (2015) Origin of microglia: current concepts and past controversies. Cold Spring Harb Perspect Biol 7(8):a020537. https:\/\/doi.org\/10.1101\/cshperspect.a020537","journal-title":"Cold Spring Harb Perspect Biol"},{"issue":"6077","key":"11_CR5","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1126\/science.1219179","volume":"336","author":"C Schulz","year":"2012","unstructured":"Schulz C, Gomez Perdiguero E, Chorro L, Szabo-Rogers H, Cagnard N, Kierdorf K, Prinz M, Wu B, Jacobsen SE, Pollard JW, Frampton J, Liu KJ, Geissmann F (2012) A lineage of myeloid cells independent of Myb and hematopoietic stem cells. Science 336(6077):86\u201390. https:\/\/doi.org\/10.1126\/science.1219179","journal-title":"Science"},{"issue":"4","key":"11_CR6","doi-asserted-by":"publisher","first-page":"779","DOI":"10.1016\/j.celrep.2017.07.004","volume":"20","author":"P Reu","year":"2017","unstructured":"Reu P, Khosravi A, Bernard S, Mold JE, Salehpour M, Alkass K, Perl S, Tisdale J, Possnert G, Druid H, Frisen J (2017) The lifespan and turnover of microglia in the human brain. Cell Rep 20(4):779\u2013784. https:\/\/doi.org\/10.1016\/j.celrep.2017.07.004","journal-title":"Cell Rep"},{"key":"11_CR7","doi-asserted-by":"publisher","DOI":"10.1038\/srep20636","volume":"6","author":"EG O\u2019Koren","year":"2016","unstructured":"O\u2019Koren EG, Mathew R, Saban DR (2016) Fate mapping reveals that microglia and recruited monocyte-derived macrophages are definitively distinguishable by phenotype in the retina. Sci Rep 6:20636. https:\/\/doi.org\/10.1038\/srep20636","journal-title":"Sci Rep"},{"issue":"5","key":"11_CR8","doi-asserted-by":"publisher","first-page":"322","DOI":"10.1038\/nri.2017.13","volume":"17","author":"NJ Reyes","year":"2017","unstructured":"Reyes NJ, O\u2019Koren EG, Saban DR (2017) New insights into mononuclear phagocyte biology from the visual system. Nat Rev Immunol 17(5):322\u2013332. https:\/\/doi.org\/10.1038\/nri.2017.13","journal-title":"Nat Rev Immunol"},{"issue":"8","key":"11_CR9","doi-asserted-by":"publisher","first-page":"1533","DOI":"10.1084\/jem.20132477","volume":"211","author":"R Yamasaki","year":"2014","unstructured":"Yamasaki R, Lu H, Butovsky O, Ohno N, Rietsch AM, Cialic R, Wu PM, Doykan CE, Lin J, Cotleur AC, Kidd G, Zorlu MM, Sun N, Hu W, Liu L, Lee JC, Taylor SE, Uehlein L, Dixon D, Gu J, Floruta CM, Zhu M, Charo IF, Weiner HL, Ransohoff RM (2014) Differential roles of microglia and monocytes in the inflamed central nervous system. J Exp Med 211(8):1533\u20131549. https:\/\/doi.org\/10.1084\/jem.20132477","journal-title":"J Exp Med"},{"issue":"12","key":"11_CR10","doi-asserted-by":"publisher","first-page":"E1738","DOI":"10.1073\/pnas.1525528113","volume":"113","author":"ML Bennett","year":"2016","unstructured":"Bennett ML, Bennett FC, Liddelow SA, Ajami B, Zamanian JL, Fernhoff NB, Mulinyawe SB, Bohlen CJ, Adil A, Tucker A, Weissman IL, Chang EF, Li G, Grant GA, Hayden Gephart MG, Barres BA (2016) New tools for studying microglia in the mouse and human CNS. Proc Natl Acad Sci U S A 113(12):E1738\u2013E1746. https:\/\/doi.org\/10.1073\/pnas.1525528113","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"11_CR11","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1038\/nn.3599","volume":"17","author":"O Butovsky","year":"2014","unstructured":"Butovsky O, Jedrychowski MP, Moore CS, Cialic R, Lanser AJ, Gabriely G, Koeglsperger T, Dake B, Wu PM, Doykan CE, Fanek Z, Liu L, Chen Z, Rothstein JD, Ransohoff RM, Gygi SP, Antel JP, Weiner HL (2014) Identification of a unique TGF-beta-dependent molecular and functional signature in microglia. Nat Neurosci 17(1):131\u2013143. https:\/\/doi.org\/10.1038\/nn.3599","journal-title":"Nat Neurosci"},{"issue":"6","key":"11_CR12","doi-asserted-by":"publisher","first-page":"1136","DOI":"10.1038\/nprot.2017.032","volume":"12","author":"A Luckoff","year":"2017","unstructured":"Luckoff A, Scholz R, Sennlaub F, Xu H, Langmann T (2017) Comprehensive analysis of mouse retinal mononuclear phagocytes. Nat Protoc 12(6):1136\u20131150. https:\/\/doi.org\/10.1038\/nprot.2017.032","journal-title":"Nat Protoc"},{"issue":"2","key":"11_CR13","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1016\/j.immuni.2017.01.006","volume":"46","author":"B Calippe","year":"2017","unstructured":"Calippe B, Augustin S, Beguier F, Charles-Messance H, Poupel L, Conart JB, Hu SJ, Lavalette S, Fauvet A, Rayes J, Levy O, Raoul W, Fitting C, Denefle T, Pickering MC, Harris C, Jorieux S, Sullivan PM, Sahel JA, Karoyan P, Sapieha P, Guillonneau X, Gautier EL, Sennlaub F (2017) Complement factor H inhibits CD47-mediated resolution of inflammation. Immunity 46(2):261\u2013272. https:\/\/doi.org\/10.1016\/j.immuni.2017.01.006","journal-title":"Immunity"},{"issue":"11","key":"11_CR14","doi-asserted-by":"publisher","first-page":"1941","DOI":"10.1007\/s00417-015-3094-z","volume":"253","author":"EM Lad","year":"2015","unstructured":"Lad EM, Cousins SW, Van Arnam JS, Proia AD (2015) Abundance of infiltrating CD163+ cells in the retina of postmortem eyes with dry and neovascular age-related macular degeneration. Graefes Arch Clin Exp Ophthalmol 253(11):1941\u20131945. https:\/\/doi.org\/10.1007\/s00417-015-3094-z","journal-title":"Graefes Arch Clin Exp Ophthalmol"},{"issue":"6","key":"11_CR15","doi-asserted-by":"publisher","first-page":"1345","DOI":"10.1189\/jlb.0207114","volume":"81","author":"T Langmann","year":"2007","unstructured":"Langmann T (2007) Microglia activation in retinal degeneration. J Leukoc Biol 81(6):1345\u20131351. https:\/\/doi.org\/10.1189\/jlb.0207114","journal-title":"J Leukoc Biol"},{"issue":"7","key":"11_CR16","doi-asserted-by":"publisher","first-page":"2977","DOI":"10.1167\/iovs.17-21672","volume":"58","author":"R Natoli","year":"2017","unstructured":"Natoli R, Fernando N, Jiao H, Racic T, Madigan M, Barnett NL, Chu-Tan JA, Valter K, Provis J, Rutar M (2017) Retinal macrophages synthesize C3 and activate complement in AMD and in models of focal retinal degeneration. Invest Ophthalmol Vis Sci 58(7):2977\u20132990. https:\/\/doi.org\/10.1167\/iovs.17-21672","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"1","key":"11_CR17","first-page":"196","volume":"37","author":"RS Roque","year":"1996","unstructured":"Roque RS, Imperial CJ, Caldwell RB (1996) Microglial cells invade the outer retina as photoreceptors degenerate in Royal College of surgeons rats. Invest Ophthalmol Vis Sci 37(1):196\u2013203","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"1","key":"11_CR18","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/0006-8993(92)91340-K","volume":"588","author":"S Thanos","year":"1992","unstructured":"Thanos S (1992) Sick photoreceptors attract activated microglia from the ganglion cell layer: a model to study the inflammatory cascades in rats with inherited retinal dystrophy. Brain Res 588(1):21\u201328","journal-title":"Brain Res"},{"issue":"9","key":"11_CR19","doi-asserted-by":"publisher","first-page":"1179","DOI":"10.15252\/emmm.201505298","volume":"7","author":"L Zhao","year":"2015","unstructured":"Zhao L, Zabel MK, Wang X, Ma W, Shah P, Fariss RN, Qian H, Parkhurst CN, Gan WB, Wong WT (2015) Microglial phagocytosis of living photoreceptors contributes to inherited retinal degeneration. EMBO Mol Med 7(9):1179\u20131197. https:\/\/doi.org\/10.15252\/emmm.201505298","journal-title":"EMBO Mol Med"},{"issue":"1","key":"11_CR20","doi-asserted-by":"publisher","first-page":"8433","DOI":"10.1038\/s41598-017-08702-7","volume":"7","author":"W Ma","year":"2017","unstructured":"Ma W, Zhang Y, Gao C, Fariss RN, Tam J, Wong WT (2017) Monocyte infiltration and proliferation reestablish myeloid cell homeostasis in the mouse retina following retinal pigment epithelial cell injury. Sci Rep 7(1):8433. https:\/\/doi.org\/10.1038\/s41598-017-08702-7","journal-title":"Sci Rep"},{"issue":"11","key":"11_CR21","doi-asserted-by":"publisher","first-page":"1618","DOI":"10.1038\/nn.3531","volume":"16","author":"T Goldmann","year":"2013","unstructured":"Goldmann T, Wieghofer P, Muller PF, Wolf Y, Varol D, Yona S, Brendecke SM, Kierdorf K, Staszewski O, Datta M, Luedde T, Heikenwalder M, Jung S, Prinz M (2013) A new type of microglia gene targeting shows TAK1 to be pivotal in CNS autoimmune inflammation. Nat Neurosci 16(11):1618\u20131626. https:\/\/doi.org\/10.1038\/nn.3531","journal-title":"Nat Neurosci"},{"issue":"7","key":"11_CR22","doi-asserted-by":"publisher","first-page":"1596","DOI":"10.1016\/j.cell.2013.11.030","volume":"155","author":"CN Parkhurst","year":"2013","unstructured":"Parkhurst CN, Yang G, Ninan I, Savas JN, Yates JR 3rd, Lafaille JJ, Hempstead BL, Littman DR, Gan WB (2013) Microglia promote learning-dependent synapse formation through brain-derived neurotrophic factor. Cell 155(7):1596\u20131609. https:\/\/doi.org\/10.1016\/j.cell.2013.11.030","journal-title":"Cell"},{"issue":"1","key":"11_CR23","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.immuni.2012.12.001","volume":"38","author":"S Yona","year":"2013","unstructured":"Yona S, Kim KW, Wolf Y, Mildner A, Varol D, Breker M, Strauss-Ayali D, Viukov S, Guilliams M, Misharin A, Hume DA, Perlman H, Malissen B, Zelzer E, Jung S (2013) Fate mapping reveals origins and dynamics of monocytes and tissue macrophages under homeostasis. Immunity 38(1):79\u201391. https:\/\/doi.org\/10.1016\/j.immuni.2012.12.001","journal-title":"Immunity"},{"issue":"3","key":"11_CR24","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0150606","volume":"11","author":"YR Yu","year":"2016","unstructured":"Yu YR, O\u2019Koren EG, Hotten DF, Kan MJ, Kopin D, Nelson ER, Que L, Gunn MD (2016) A protocol for the comprehensive flow Cytometric analysis of immune cells in normal and inflamed murine non-lymphoid tissues. PLoS One 11(3):e0150606. https:\/\/doi.org\/10.1371\/journal.pone.0150606","journal-title":"PLoS One"},{"key":"11_CR25","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.exer.2012.08.013","volume":"104","author":"C Guo","year":"2012","unstructured":"Guo C, Otani A, Oishi A, Kojima H, Makiyama Y, Nakagawa S, Yoshimura N (2012) Knockout of ccr2 alleviates photoreceptor cell death in a model of retinitis pigmentosa. Exp Eye Res 104:39\u201347. https:\/\/doi.org\/10.1016\/j.exer.2012.08.013","journal-title":"Exp Eye Res"},{"issue":"23","key":"11_CR26","doi-asserted-by":"publisher","first-page":"E3040","DOI":"10.1073\/pnas.1424391112","volume":"112","author":"CB Toomey","year":"2015","unstructured":"Toomey CB, Kelly U, Saban DR, Bowes Rickman C (2015) Regulation of age-related macular degeneration-like pathology by complement factor H. Proc Natl Acad Sci U S A 112(23):E3040\u2013E3049. https:\/\/doi.org\/10.1073\/pnas.1424391112","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"3","key":"11_CR27","doi-asserted-by":"publisher","first-page":"599","DOI":"10.1016\/j.immuni.2013.08.007","volume":"39","author":"C Jakubzick","year":"2013","unstructured":"Jakubzick C, Gautier EL, Gibbings SL, Sojka DK, Schlitzer A, Johnson TE, Ivanov S, Duan Q, Bala S, Condon T, van Rooijen N, Grainger JR, Belkaid Y, Ma\u2019ayan A, Riches DW, Yokoyama WM, Ginhoux F, Henson PM, Randolph GJ (2013) Minimal differentiation of classical monocytes as they survey steady-state tissues and transport antigen to lymph nodes. Immunity 39(3):599\u2013610. https:\/\/doi.org\/10.1016\/j.immuni.2013.08.007","journal-title":"Immunity"},{"issue":"12","key":"11_CR28","doi-asserted-by":"publisher","first-page":"1544","DOI":"10.1038\/nn2015","volume":"10","author":"A Mildner","year":"2007","unstructured":"Mildner A, Schmidt H, Nitsche M, Merkler D, Hanisch UK, Mack M, Heikenwalder M, Bruck W, Priller J, Prinz M (2007) Microglia in the adult brain arise from Ly-6ChiCCR2+ monocytes only under defined host conditions. Nat Neurosci 10(12):1544\u20131553. https:\/\/doi.org\/10.1038\/nn2015","journal-title":"Nat Neurosci"},{"issue":"11","key":"11_CR29","doi-asserted-by":"publisher","first-page":"1189","DOI":"10.1002\/glia.20535","volume":"55","author":"H Xu","year":"2007","unstructured":"Xu H, Chen M, Mayer EJ, Forrester JV, Dick AD (2007) Turnover of resident retinal microglia in the normal adult mouse. Glia 55(11):1189\u20131198. https:\/\/doi.org\/10.1002\/glia.20535","journal-title":"Glia"},{"issue":"6","key":"11_CR30","doi-asserted-by":"publisher","first-page":"670","DOI":"10.15252\/emmm.201505994","volume":"8","author":"A Luckoff","year":"2016","unstructured":"Luckoff A, Caramoy A, Scholz R, Prinz M, Kalinke U, Langmann T (2016) Interferon-beta signaling in retinal mononuclear phagocytes attenuates pathological neovascularization. EMBO Mol Med 8(6):670\u2013678. https:\/\/doi.org\/10.15252\/emmm.201505994","journal-title":"EMBO Mol Med"}],"container-title":["Methods in Molecular Biology","Retinal Degeneration"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-4939-8669-9_11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T02:02:48Z","timestamp":1580090568000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-4939-8669-9_11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019]]},"ISBN":["9781493986682","9781493986699"],"references-count":30,"URL":"https:\/\/doi.org\/10.1007\/978-1-4939-8669-9_11","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019]]}}}