{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T04:13:34Z","timestamp":1791000814260,"version":"4.1.0"},"publisher-location":"New York, NY","reference-count":15,"publisher":"Springer New York","isbn-type":[{"value":"9781493987290","type":"print"},{"value":"9781493987306","type":"electronic"}],"license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018]]},"DOI":"10.1007\/978-1-4939-8730-6_12","type":"book-chapter","created":{"date-parts":[[2018,9,21]],"date-time":"2018-09-21T16:23:51Z","timestamp":1537547031000},"page":"179-187","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["High Throughput Transfection of HEK293 Cells for Transient Protein Production"],"prefix":"10.1007","author":[{"given":"Tia A.","family":"Arena","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter D.","family":"Harms","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Athena W.","family":"Wong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,9,22]]},"reference":[{"key":"12_CR1","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1016\/S0958-1669(99)80027-5","volume":"10","author":"F Wurm","year":"1999","unstructured":"Wurm F, Bernard A (1999) Large-scale transient expression in mammalian cells for recombinant protein production. Curr Opin Biotechnol 10:156\u2013159. \n                  https:\/\/doi.org\/10.1016\/S0958-1669(99)80027-5","journal-title":"Curr Opin Biotechnol"},{"key":"12_CR2","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1007\/s10529-006-9297-y","volume":"29","author":"L Baldi","year":"2007","unstructured":"Baldi L, Hacker DL, Adam M, Wurm FM (2007) Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives. Biotechnol Lett 29:677\u2013684. \n                  https:\/\/doi.org\/10.1007\/s10529-006-9297-y","journal-title":"Biotechnol Lett"},{"key":"12_CR3","doi-asserted-by":"publisher","first-page":"1832","DOI":"10.1002\/bit.25601","volume":"112","author":"AB Bos","year":"2015","unstructured":"Bos AB, Luan P, Duque JN, Reilly D, Harms PD, Wong AW (2015) Optimization and automation of an end-to-end high throughput microscale transient protein production process. Biotechnol Bioeng 112:1832\u20131842. \n                  https:\/\/doi.org\/10.1002\/bit.25601","journal-title":"Biotechnol Bioeng"},{"key":"12_CR4","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1016\/j.jsb.2011.04.017","volume":"175","author":"Y Zhao","year":"2011","unstructured":"Zhao Y, Bishop B, Clay JE, Lu W, Jones M, Daenke S, Siebold C, Stuart DI, Jones EY, Aricescu AR (2011) Automation of large scale transient protein expression in mammalian cells. J Struct Biol 175:209\u2013215. \n                  https:\/\/doi.org\/10.1016\/j.jsb.2011.04.017","journal-title":"J Struct Biol"},{"key":"12_CR5","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1016\/j.ymeth.2013.07.018","volume":"65","author":"T Vink","year":"2014","unstructured":"Vink T, Oudshoorn-Dickmann M, Roza M, Reitsma JJ, de Jong RN (2014) A simple, robust and highly efficient transient expression system for producing antibodies. Methods 65:5\u201310. \n                  https:\/\/doi.org\/10.1016\/j.ymeth.2013.07.018","journal-title":"Methods"},{"key":"12_CR6","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1099\/0022-1317-36-1-59","volume":"36","author":"FL Graham","year":"1977","unstructured":"Graham FL, Smiley J, Russell WC, Nairn R (1977) Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J Gen Virol 36:59\u201374. \n                  https:\/\/doi.org\/10.1099\/0022-1317-36-1-59","journal-title":"J Gen Virol"},{"key":"12_CR7","doi-asserted-by":"publisher","first-page":"336","DOI":"10.1016\/j.jbiotec.2012.06.038","volume":"161","author":"A Croset","year":"2012","unstructured":"Croset A, Delafosse L, Gaudry JP, Arod C, Glez L, Losberger C, Begue D, Krstanovic A, Robert F, Vilbois F, Chevalet L, Antonsson B (2012) Differences in the glycosylation of recombinant proteins expressed in HEK and CHO cells. J Biotechnol 161:336\u2013348. \n                  https:\/\/doi.org\/10.1016\/j.jbiotec.2012.06.038","journal-title":"J Biotechnol"},{"key":"12_CR8","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1016\/j.pep.2017.03.018","volume":"134","author":"NK Jain","year":"2017","unstructured":"Jain NK, Barkowski-Clark S, Altman R, Johnson K, Sun F, Zmuda J, Liu CY, Kita A, Schulz R, Neill A, Ballinger R, Patel R, Liu J, Mpanda A, Huta B, Chiou H, Voegtli W, Panavas T (2017) A high density CHO-S transient transfection system: comparison of ExpiCHO and Expi293. Protein Expr Purif 134:38\u201346. \n                  https:\/\/doi.org\/10.1016\/j.pep.2017.03.018","journal-title":"Protein Expr Purif"},{"key":"12_CR9","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1002\/btpr.2375","volume":"33","author":"LK Zhu","year":"2017","unstructured":"Zhu LK, Song BY, Wang ZL, Monteil DT, Shen X, Hacker DL, De Jesus M, Wurm FM (2017) Studies on fluid dynamics of the flow field and gas transfer in orbitally shaken tubes. Biotechnol Prog 33:192\u2013200. \n                  https:\/\/doi.org\/10.1002\/btpr.2375","journal-title":"Biotechnol Prog"},{"key":"12_CR10","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.pep.2011.02.008","volume":"78","author":"F Bollin","year":"2011","unstructured":"Bollin F, Dechavanne V, Chevalet L (2011) Design of Experiment in CHO and HEK transient transfection condition optimization. Protein Expr Purif 78:61\u201368. \n                  https:\/\/doi.org\/10.1016\/j.pep.2011.02.008","journal-title":"Protein Expr Purif"},{"key":"12_CR11","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1016\/j.ejps.2010.03.019","volume":"40","author":"C Tros de Ilarduya","year":"2010","unstructured":"Tros de Ilarduya C, Sun Y, Duzgunes N (2010) Gene delivery by lipoplexes and polyplexes. Eur J Pharm Sci 40:159\u2013170. \n                  https:\/\/doi.org\/10.1016\/j.ejps.2010.03.019","journal-title":"Eur J Pharm Sci"},{"key":"12_CR12","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.jbiotec.2014.03.027","volume":"180","author":"AB Bos","year":"2014","unstructured":"Bos AB, Duque JN, Bhakta S, Farahi F, Chirdon LA, Junutula JR, Harms PD, Wong AW (2014) Development of a semi-automated high throughput transient transfection system. J Biotechnol 180:10\u201316. \n                  https:\/\/doi.org\/10.1016\/j.jbiotec.2014.03.027","journal-title":"J Biotechnol"},{"key":"12_CR13","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1002\/bit.21882","volume":"101","author":"G Backliwal","year":"2008","unstructured":"Backliwal G, Hildinger M, Kuettel I, Delegrange F, Hacker DL, Wurm FM (2008) Valproic acid: a viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures. Biotechnol Bioeng 101:182\u2013189. \n                  https:\/\/doi.org\/10.1002\/bit.21882","journal-title":"Biotechnol Bioeng"},{"key":"12_CR14","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1002\/bit.21726","volume":"100","author":"Z Jiang","year":"2008","unstructured":"Jiang Z, Sharfstein ST (2008) Sodium butyrate stimulates monoclonal antibody over-expression in CHO cells by improving gene accessibility. Biotechnol Bioeng 100:189\u2013194. \n                  https:\/\/doi.org\/10.1002\/bit.21726","journal-title":"Biotechnol Bioeng"},{"key":"12_CR15","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1016\/j.jbiotec.2010.05.003","volume":"148","author":"S Wulhfard","year":"2010","unstructured":"Wulhfard S, Baldi L, Hacker DL, Wurm F (2010) Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. J Biotechnol 148:128\u2013132. \n                  https:\/\/doi.org\/10.1016\/j.jbiotec.2010.05.003","journal-title":"J Biotechnol"}],"container-title":["Methods in Molecular Biology","Recombinant Protein Expression in Mammalian Cells"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-4939-8730-6_12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T07:30:24Z","timestamp":1583220624000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-4939-8730-6_12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"ISBN":["9781493987290","9781493987306"],"references-count":15,"URL":"https:\/\/doi.org\/10.1007\/978-1-4939-8730-6_12","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018]]},"assertion":[{"value":"22 September 2018","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}