{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T03:20:34Z","timestamp":1782271234837,"version":"3.54.5"},"publisher-location":"New York, NY","reference-count":11,"publisher":"Springer New York","isbn-type":[{"value":"9781493995899","type":"print"},{"value":"9781493995912","type":"electronic"}],"license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019]]},"DOI":"10.1007\/978-1-4939-9591-2_12","type":"book-chapter","created":{"date-parts":[[2019,6,8]],"date-time":"2019-06-08T10:02:41Z","timestamp":1559988161000},"page":"165-174","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Isolation and Characterization of Lymphocytes from Human Mucosal Biopsies"],"prefix":"10.1007","author":[{"given":"Nadine E. G.","family":"Upton","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas S.","family":"Hayday","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louisa K.","family":"James","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,6,9]]},"reference":[{"key":"12_CR1","doi-asserted-by":"publisher","first-page":"406","DOI":"10.1111\/j.1365-2559.2006.02514.x","volume":"49","author":"RA Walker","year":"2006","unstructured":"Walker RA (2006) Quantification of immunohistochemistry-issues concerning methods, utility and semiquantitative assessment II. Histopathology 49:406\u2013410","journal-title":"Histopathology"},{"key":"12_CR2","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1111\/j.1365-2559.2006.02513.x","volume":"49","author":"CR Taylor","year":"2006","unstructured":"Taylor CR, Levenson RM (2006) Quantification of immunohistochemistry-issues concerning methods, utility and semiquantitative assessment II. Histopathology 49:411\u2013424","journal-title":"Histopathology"},{"key":"12_CR3","doi-asserted-by":"publisher","first-page":"988","DOI":"10.1177\/0192623311419163","volume":"39","author":"RW Dunstan","year":"2011","unstructured":"Dunstan RW, Wharton KA, Quigley C et al (2011) The use of immunohistochemistry for biomarker assessment-can it compete with other technologies? Toxicol Pathol 39:988\u20131002","journal-title":"Toxicol Pathol"},{"key":"12_CR4","doi-asserted-by":"publisher","first-page":"S248","DOI":"10.1016\/S0091-6749(96)70073-8","volume":"98","author":"R Pawankar","year":"1996","unstructured":"Pawankar R, Ra C (1996) Heterogeneity of mast cells and T cells in the nasal mucosa. J Allergy Clin Immunol 98:S248\u2013S262","journal-title":"J Allergy Clin Immunol"},{"key":"12_CR5","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/S0022-1759(00)00229-5","volume":"243","author":"N Baumgarth","year":"2000","unstructured":"Baumgarth N, Roederer M (2000) A practical approach to multicolor flow cytometry for immunophenotyping. J Immunol Methods 243:77\u201397","journal-title":"J Immunol Methods"},{"key":"12_CR6","doi-asserted-by":"publisher","first-page":"767","DOI":"10.2353\/ajpath.2007.061177","volume":"171","author":"CM Freeman","year":"2007","unstructured":"Freeman CM, Curtis JL, Chensue SW (2007) CC chemokine receptor 5 and CXC chemokine receptor 6 expression by lung CD8+ cells correlates with chronic obstructive pulmonary disease severity. Am J Pathol 171:767\u2013776","journal-title":"Am J Pathol"},{"key":"12_CR7","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1016\/0022-1759(92)90307-F","volume":"153","author":"CE Havenith","year":"1992","unstructured":"Havenith CE, Breedijk AJ, Verdaasdonk MA et al (1992) An improved and rapid method for the isolation of rat lymph node or spleen T lymphocytes for T cell proliferation assays. J Immunol Methods 153:73\u201379","journal-title":"J Immunol Methods"},{"key":"12_CR8","unstructured":"https:\/\/lifescience.roche.com\/shop\/en\/es\/overviews\/brand\/tissue-dissociation-overview"},{"key":"12_CR9","doi-asserted-by":"publisher","first-page":"560","DOI":"10.1111\/j.1365-2249.2010.04198.x","volume":"161","author":"MW Lethbridge","year":"2010","unstructured":"Lethbridge MW, Kemeny DM, Ratoff JC et al (2010) A novel technique to explore the functions of bronchial mucosal T cells in chronic obstructive pulmonary disease: application to cytotoxicity and cytokine immunoreactivity. Clin Exp Immunol 161:560\u2013569","journal-title":"Clin Exp Immunol"},{"key":"12_CR10","doi-asserted-by":"publisher","first-page":"1410","DOI":"10.1056\/NEJMoa064691","volume":"356","author":"P Vijayanand","year":"2007","unstructured":"Vijayanand P, Seumois G, Pickard C et al (2007) Invariant natural killer T cells in asthma and chronic obstructive pulmonary disease. N Engl J Med 356:1410\u20131422","journal-title":"N Engl J Med"},{"key":"12_CR11","doi-asserted-by":"publisher","first-page":"3610","DOI":"10.4049\/jimmunol.167.7.3610","volume":"167","author":"J\u00d8 Bohnhorst","year":"2001","unstructured":"Bohnhorst J\u00d8, Bj\u00f8rgan MB, Thoen JE et al (2001) Bm1\u2013Bm5 classification of peripheral blood B cells reveals circulating germinal center founder cells in healthy individuals and disturbance in the B cell subpopulations in patients with primary Sj\u00f6gren\u2019s syndrome. J Immunol 167:3610\u20133618","journal-title":"J Immunol"}],"container-title":["Methods in Molecular Biology","Allergy"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-4939-9591-2_12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,6,8]],"date-time":"2019-06-08T10:03:07Z","timestamp":1559988187000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-4939-9591-2_12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019]]},"ISBN":["9781493995899","9781493995912"],"references-count":11,"URL":"https:\/\/doi.org\/10.1007\/978-1-4939-9591-2_12","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019]]},"assertion":[{"value":"9 June 2019","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}