{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T14:55:55Z","timestamp":1793199355722,"version":"4.5.0"},"publisher-location":"New York, NY","reference-count":18,"publisher":"Springer New York","isbn-type":[{"value":"9781493996346","type":"print"},{"value":"9781493996353","type":"electronic"}],"license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019]]},"DOI":"10.1007\/978-1-4939-9635-3_13","type":"book-chapter","created":{"date-parts":[[2019,6,21]],"date-time":"2019-06-21T12:02:38Z","timestamp":1561118558000},"page":"231-246","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Design, Synthesis, and Functional Analysis of Highly Specific Artificial Small RNAs with Antiviral Activity in Plants"],"prefix":"10.1007","author":[{"given":"Alberto","family":"Carbonell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9-Antonio","family":"Dar\u00f2s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,6,22]]},"reference":[{"key":"13_CR1","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1079\/9781780647678.0110","volume-title":"Plant gene silencing: mechanisms and applications","author":"A Carbonell","year":"2017","unstructured":"Carbonell A (2017) Artificial small RNA-based strategies for effective and specific gene silencing in plants. In: Dalmay T (ed) Plant gene silencing: mechanisms and applications. CABI Publishing, Boston, MA, pp 110\u2013127"},{"key":"13_CR2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/978-1-4939-7165-7_1","volume":"1640","author":"A Carbonell","year":"2017","unstructured":"Carbonell A (2017) Plant ARGONAUTEs: features, functions, and unknowns. Methods Mol Biol 1640:1\u201321. \n                  https:\/\/doi.org\/10.1007\/978-1-4939-7165-7_1","journal-title":"Methods Mol Biol"},{"issue":"11","key":"13_CR3","doi-asserted-by":"publisher","first-page":"1420","DOI":"10.1038\/nbt1255","volume":"24","author":"QW Niu","year":"2006","unstructured":"Niu QW, Lin SS, Reyes JL, Chen KC, Wu HW, Yeh SD, Chua NH (2006) Expression of artificial microRNAs in transgenic Arabidopsis thaliana confers virus resistance. Nat Biotechnol 24(11):1420\u20131428. \n                  https:\/\/doi.org\/10.1038\/nbt1255","journal-title":"Nat Biotechnol"},{"issue":"2","key":"13_CR4","doi-asserted-by":"publisher","first-page":"e1000312","DOI":"10.1371\/journal.ppat.1000312","volume":"5","author":"SS Lin","year":"2009","unstructured":"Lin SS, Wu HW, Elena SF, Chen KC, Niu QW, Yeh SD, Chen CC, Chua NH (2009) Molecular evolution of a viral non-coding sequence under the selective pressure of amiRNA-mediated silencing. PLoS Pathog 5(2):e1000312. \n                  https:\/\/doi.org\/10.1371\/journal.ppat.1000312","journal-title":"PLoS Pathog"},{"issue":"19","key":"13_CR5","doi-asserted-by":"publisher","first-page":"9686","DOI":"10.1128\/JVI.05326-11","volume":"85","author":"G Lafforgue","year":"2011","unstructured":"Lafforgue G, Martinez F, Sardanyes J, de la Iglesia F, Niu QW, Lin SS, Sole RV, Chua NH, Daros JA, Elena SF (2011) Tempo and mode of plant RNA virus escape from RNA interference-mediated resistance. J Virol 85(19):9686\u20139695. \n                  https:\/\/doi.org\/10.1128\/JVI.05326-11","journal-title":"J Virol"},{"issue":"2","key":"13_CR6","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1111\/j.1467-7652.2011.00647.x","volume":"10","author":"M Fahim","year":"2012","unstructured":"Fahim M, Millar AA, Wood CC, Larkin PJ (2012) Resistance to Wheat streak mosaic virus generated by expression of an artificial polycistronic microRNA in wheat. Plant Biotechnol J 10(2):150\u2013163. \n                  https:\/\/doi.org\/10.1111\/j.1467-7652.2011.00647.x","journal-title":"Plant Biotechnol J"},{"issue":"3","key":"13_CR7","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1111\/j.1364-3703.2011.00747.x","volume":"13","author":"YJ Kung","year":"2012","unstructured":"Kung YJ, Lin SS, Huang YL, Chen TC, Harish SS, Chua NH, Yeh SD (2012) Multiple artificial microRNAs targeting conserved motifs of the replicase gene confer robust transgenic resistance to negative-sense single-stranded RNA plant virus. Mol Plant Pathol 13(3):303\u2013317. \n                  https:\/\/doi.org\/10.1111\/j.1364-3703.2011.00747.x","journal-title":"Mol Plant Pathol"},{"issue":"14","key":"13_CR8","doi-asserted-by":"publisher","first-page":"8254","DOI":"10.1128\/JVI.00914-13","volume":"87","author":"G Lafforgue","year":"2013","unstructured":"Lafforgue G, Martinez F, Niu QW, Chua NH, Daros JA, Elena SF (2013) Improving the effectiveness of artificial microRNA (amiR)-mediated resistance against Turnip mosaic virus by combining two amiRs or by targeting highly conserved viral genomic regions. J Virol 87(14):8254\u20138256. \n                  https:\/\/doi.org\/10.1128\/JVI.00914-13","journal-title":"J Virol"},{"issue":"3","key":"13_CR9","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1111\/mpp.12291","volume":"17","author":"A Kis","year":"2016","unstructured":"Kis A, Tholt G, Ivanics M, Varallyay E, Jenes B, Havelda Z (2016) Polycistronic artificial miRNA-mediated resistance to Wheat dwarf virus in barley is highly efficient at low temperature. Mol Plant Pathol 17(3):427\u2013437. \n                  https:\/\/doi.org\/10.1111\/mpp.12291","journal-title":"Mol Plant Pathol"},{"key":"13_CR10","unstructured":"Carbonell A, Carrington JC, Daros JA (2016) Fast-forward generation of effective artificial small RNAs for enhanced antiviral defense in plants. RNA Dis 3(1)"},{"key":"13_CR11","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.jviromet.2015.11.004","volume":"228","author":"L Chen","year":"2016","unstructured":"Chen L, Cheng X, Cai J, Zhan L, Wu X, Liu Q, Wu X (2016) Multiple virus resistance using artificial trans-acting siRNAs. J Virol Methods 228:16\u201320. \n                  https:\/\/doi.org\/10.1016\/j.jviromet.2015.11.004","journal-title":"J Virol Methods"},{"issue":"5","key":"13_CR12","doi-asserted-by":"publisher","first-page":"746","DOI":"10.1111\/mpp.12529","volume":"18","author":"A Carbonell","year":"2017","unstructured":"Carbonell A, Daros JA (2017) Artificial microRNAs and synthetic trans-acting small interfering RNAs interfere with viroid infection. Mol Plant Pathol 18(5):746\u2013753. \n                  https:\/\/doi.org\/10.1111\/mpp.12529","journal-title":"Mol Plant Pathol"},{"issue":"1","key":"13_CR13","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1104\/pp.113.234989","volume":"165","author":"A Carbonell","year":"2014","unstructured":"Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, Carrington JC (2014) New generation of artificial MicroRNA and synthetic trans-acting small interfering RNA vectors for efficient gene silencing in Arabidopsis. Plant Physiol 165(1):15\u201329. \n                  https:\/\/doi.org\/10.1104\/pp.113.234989","journal-title":"Plant Physiol"},{"issue":"6","key":"13_CR14","doi-asserted-by":"publisher","first-page":"1061","DOI":"10.1111\/tpj.12835","volume":"82","author":"A Carbonell","year":"2015","unstructured":"Carbonell A, Fahlgren N, Mitchell S, Cox KL Jr, Reilly KC, Mockler TC, Carrington JC (2015) Highly specific gene silencing in a monocot species by artificial microRNAs derived from chimeric miRNA precursors. Plant J 82(6):1061\u20131075. \n                  https:\/\/doi.org\/10.1111\/tpj.12835","journal-title":"Plant J"},{"issue":"1","key":"13_CR15","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1093\/bioinformatics\/btv534","volume":"32","author":"N Fahlgren","year":"2016","unstructured":"Fahlgren N, Hill ST, Carrington JC, Carbonell A (2016) P-SAMS: a web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design. Bioinformatics 32(1):157\u2013158. \n                  https:\/\/doi.org\/10.1093\/bioinformatics\/btv534","journal-title":"Bioinformatics"},{"issue":"2","key":"13_CR16","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1094\/MPMI-05-18-0117-TA","volume":"32","author":"Alberto Carbonell","year":"2019","unstructured":"Carbonell A, L\u00f3pez C, Dar\u00f2s JA (2019) Fast-forward identification of highly effective artificial small RNAs against different Tomato spotted wilt virus isolates. Mol Plant-Microbe Interact 32(2):142\u2013156. \n                  https:\/\/doi.org\/10.1094\/MPMI-05-18-0117-TA","journal-title":"Molecular Plant-Microbe Interactions"},{"issue":"1","key":"13_CR17","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1016\/j.cell.2008.02.033","volume":"133","author":"Taiowa A. Montgomery","year":"2008","unstructured":"Montgomery TA, Howell MD, Cuperus JT, Li D, Hansen JE, Alexander AL, Chapman EJ, Fahlgren N, Allen E, Carrington JC (2008) Specificity of ARGONAUTE7-miR390 interaction and dual functionality in TAS3 trans-acting siRNA formation. Cell 133(1):128\u2013141. \n                  https:\/\/doi.org\/10.1016\/j.cell.2008.02.033","journal-title":"Cell"},{"key":"13_CR18","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1016\/j.virusres.2015.10.003","volume":"211","author":"Neena Mitter","year":"2016","unstructured":"Mitter N, Zhai Y, Bai AX, Chua K, Eid S, Constantin M, Mitchell R, Pappu HR (2016) Evaluation and identification of candidate genes for artificial microRNA-mediated resistance to tomato spotted wilt virus. Virus Res 211:151\u2013158.\u00a0\n                  https:\/\/doi.org\/10.1016\/j.virusres.2015.10.003","journal-title":"Virus Research"}],"container-title":["Methods in Molecular Biology","Antiviral Resistance in Plants"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-4939-9635-3_13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,6,21]],"date-time":"2019-06-21T12:03:10Z","timestamp":1561118590000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-4939-9635-3_13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019]]},"ISBN":["9781493996346","9781493996353"],"references-count":18,"URL":"https:\/\/doi.org\/10.1007\/978-1-4939-9635-3_13","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019]]},"assertion":[{"value":"22 June 2019","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}