{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T01:20:44Z","timestamp":1791595244494,"version":"4.3.4"},"publisher-location":"New York, NY","reference-count":12,"publisher":"Springer US","isbn-type":[{"value":"9781493998487","type":"print"},{"value":"9781493998494","type":"electronic"}],"license":[{"start":{"date-parts":[[2019,9,16]],"date-time":"2019-09-16T00:00:00Z","timestamp":1568592000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020]]},"DOI":"10.1007\/978-1-4939-9849-4_12","type":"book-chapter","created":{"date-parts":[[2019,9,15]],"date-time":"2019-09-15T11:03:46Z","timestamp":1568545426000},"page":"155-175","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Identification of Virulence Determinants During Host-Pathogen Interaction Using Tn-Seq Technology"],"prefix":"10.1007","author":[{"given":"Christopher T.","family":"Peek","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn B.","family":"Ibberson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James E.","family":"Cassat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,9,16]]},"reference":[{"key":"12_CR1","doi-asserted-by":"publisher","first-page":"12312","DOI":"10.1073\/pnas.0404728101","volume":"101","author":"T Bae","year":"2004","unstructured":"Bae T, Banger AK, Wallace A, Glass EM, Aslund F, Schneewind O et al (2004) Staphylococcus aureus virulence genes identified by bursa aurealis mutagenesis and nematode killing. Proc Natl Acad Sci U S A 101:12312\u201312317. \n                  https:\/\/doi.org\/10.1073\/pnas.0404728101","journal-title":"Proc Natl Acad Sci U S A"},{"key":"12_CR2","doi-asserted-by":"publisher","first-page":"e01729","DOI":"10.1128\/mBio.01729-14","volume":"5","author":"MD Valentino","year":"2014","unstructured":"Valentino MD, Foulston L, Sadaka A, Kos VN, Villet RA, Santa Maria J Jr et al (2014) Genes contributing to Staphylococcus aureus fitness in abscess- and infection-related ecologies. mBio 5:e01729\u2013e01714. \n                  https:\/\/doi.org\/10.1128\/mBio.01729-14","journal-title":"mBio"},{"key":"12_CR3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005341","volume":"11","author":"AD Wilde","year":"2015","unstructured":"Wilde AD, Snyder DJ, Putnam NE, Valentino MD, Hammer ND, Lonergan ZR et al (2015) Bacterial hypoxic responses revealed as critical determinants of the host-pathogen outcome by TnSeq analysis of Staphylococcus aureus invasive infection. PLoS Pathog 11:e1005341. \n                  https:\/\/doi.org\/10.1371\/journal.ppat.1005341","journal-title":"PLoS Pathog"},{"key":"12_CR4","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006907","volume":"14","author":"MR Grosser","year":"2018","unstructured":"Grosser MR, Paluscio E, Thurlow LR, Dillon MM, Cooper VS, Kawula TH et al (2018) Genetic requirements for Staphylococcus aureus nitric oxide resistance and virulence. PLoS Pathog 14:e1006907. \n                  https:\/\/doi.org\/10.1371\/journal.ppat.1006907","journal-title":"PLoS Pathog"},{"key":"12_CR5","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2017.79","volume":"2","author":"CB Ibberson","year":"2017","unstructured":"Ibberson CB, Stacy A, Fleming D, Dees JL, Rumbaugh K, Gilmore MS et al (2017) Co-infecting microorganisms dramatically alter pathogen gene essentiality during polymicrobial infection. Nat Microbiol 2:17079. \n                  https:\/\/doi.org\/10.1038\/nmicrobiol.2017.79","journal-title":"Nat Microbiol"},{"key":"12_CR6","doi-asserted-by":"publisher","first-page":"837","DOI":"10.2106\/JBJS.N.01350","volume":"97","author":"HM Kremers","year":"2015","unstructured":"Kremers HM, Nwojo ME, Ransom JE, Wood-Wentz CM, Melton LJ, Huddleston PM (2015) Trends in the epidemiology of osteomyelitis: a population-based study, 1969 to 2009. J Bone Joint Surg Am 97:837\u2013845. \n                  https:\/\/doi.org\/10.2106\/JBJS.N.01350","journal-title":"J Bone Joint Surg Am"},{"key":"12_CR7","doi-asserted-by":"publisher","first-page":"759","DOI":"10.1016\/j.chom.2013.05.003","volume":"13","author":"JE Cassat","year":"2013","unstructured":"Cassat JE, Hammer ND, Campbell JP, Benson MA, Perrien DS, Mrak LN et al (2013) A secreted bacterial protease tailors the Staphylococcus aureus virulence repertoire to modulate bone remodeling during osteomyelitis. Cell Host Microbe 13:759\u2013772. \n                  https:\/\/doi.org\/10.1016\/j.chom.2013.05.003","journal-title":"Cell Host Microbe"},{"key":"12_CR8","doi-asserted-by":"publisher","first-page":"4110","DOI":"10.1073\/pnas.1419677112","volume":"112","author":"KH Turner","year":"2015","unstructured":"Turner KH, Wessel AK, Palmer GC, Murray JL, Whiteley M (2015) Essential genome of Pseudomonas aeruginosa in cystic fibrosis sputum. Proc Natl Acad Sci U S A 112:4110\u20134115. \n                  https:\/\/doi.org\/10.1073\/pnas.1419677112","journal-title":"Proc Natl Acad Sci U S A"},{"key":"12_CR9","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1007\/978-1-59745-321-9_7","volume-title":"Microbial gene essentiality: protocols and bioinformatics","author":"T Bae","year":"2008","unstructured":"Bae T, Glass EM, Schneewind O, Missiakas D (2008) Generating a collection of insertion mutations in the Staphylococcus aureus genome using bursa aurealis. In: Osterman AL, Gerdes SY (eds) Microbial gene essentiality: protocols and bioinformatics. Humana Press, Totowa, NJ, pp 103\u2013116"},{"key":"12_CR10","first-page":"69","volume-title":"The genetic manipulation of Staphylococci: methods and protocols","author":"ME Olson","year":"2016","unstructured":"Olson ME (2016) Bacteriophage transduction in Staphylococcus aureus. In: Bose JL (ed) The genetic manipulation of Staphylococci: methods and protocols. Springer New York, New York, NY, pp 69\u201374"},{"key":"12_CR11","doi-asserted-by":"publisher","first-page":"10","DOI":"10.14806\/ej.17.1.200","volume":"17","author":"M Martin","year":"2011","unstructured":"Martin M (2011) Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet J 17:10\u201312. \n                  https:\/\/doi.org\/10.14806\/ej.17.1.200","journal-title":"EMBnet J"},{"key":"12_CR12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043012","volume":"7","author":"A Zomer","year":"2012","unstructured":"Zomer A, Burghout P, Bootsma HJ, Hermans PW, van Hijum SA (2012) ESSENTIALS: software for rapid analysis of high throughput transposon insertion sequencing data. PLoS One 7:e43012. \n                  https:\/\/doi.org\/10.1371\/journal.pone.0043012","journal-title":"PLoS One"}],"container-title":["Methods in Molecular Biology","Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-4939-9849-4_12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,15]],"date-time":"2019-09-15T11:04:14Z","timestamp":1568545454000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-4939-9849-4_12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,16]]},"ISBN":["9781493998487","9781493998494"],"references-count":12,"URL":"https:\/\/doi.org\/10.1007\/978-1-4939-9849-4_12","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,16]]},"assertion":[{"value":"16 September 2019","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}