{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T03:16:41Z","timestamp":1778642201032,"version":"3.51.4"},"publisher-location":"Totowa, NJ","reference-count":35,"publisher":"Humana Press","isbn-type":[{"value":"9781603273244","type":"print"},{"value":"9781603273251","type":"electronic"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010]]},"DOI":"10.1007\/978-1-60327-325-1_2","type":"book-chapter","created":{"date-parts":[[2010,5,26]],"date-time":"2010-05-26T20:04:33Z","timestamp":1274904273000},"page":"55-73","source":"Crossref","is-referenced-by-count":20,"title":["Culture of Highly Differentiated Human Retinal Pigment Epithelium for Analysis of the Polarized Uptake, Processing, and Secretion of Retinoids"],"prefix":"10.1007","author":[{"given":"Jane","family":"Hu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dean","family":"Bok","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2010,5,17]]},"reference":[{"key":"2_CR1","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1038\/ng0397-236","volume":"15","author":"R. Allikmets","year":"1997","unstructured":"Allikmets, R., Singh, N., Sun, H., et al. (1997) A photoreceptor cell-specific ATP-binding transporter gene (ABCR) is mutated in recessive Stargardt macular dystrophy. Nat. Genet. 15, 236\u2013246.","journal-title":"Nat. Genet"},{"key":"2_CR2","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/S0092-8674(00)80602-9","volume":"98","author":"J. Weng","year":"1999","unstructured":"Weng, J., Mata, N.L., Azarian, S.M., Tzekov, R.T. Birch, D.G., Travis, G.H. (1999) Insights into the function of Rim protein in photoreceptors and etiology of Stargardt\u2019s disease from the phenotype in abcr knockout mice. Cell 98, 13\u201323.","journal-title":"Cell"},{"key":"2_CR3","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1038\/ng1097-139","volume":"17","author":"F. Marlhens","year":"1997","unstructured":"Marlhens, F., Bareil, D., Griffoin, J-M, et al. (1997) Mutations in RPE65 cause Leber\u2019s congenital Amaurosis. Nat. Genet. 17, 139\u2013141.","journal-title":"Nat. Genet"},{"key":"2_CR4","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1038\/3813","volume":"20","author":"T.M. Redmond","year":"1998","unstructured":"Redmond, T.M., Yu, S., Lee, E., et al. (1998) Rpe65 is necessary for production of 11-cis-vitamin A in the retinal visual cycle. Nat. Genet. 20, 344\u2013351.","journal-title":"Nat. Genet"},{"key":"2_CR5","doi-asserted-by":"publisher","first-page":"820","DOI":"10.1038\/ng0708-820","volume":"40","author":"R. Allikmets","year":"2008","unstructured":"Allikmets, R., Dean, M. (2008) Bringing age-related macular degeneration into focus. Nat. Genet. 40, 820\u2013821.","journal-title":"Nat. Genet"},{"key":"2_CR6","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1001\/archophthalmol.2010.18","volume":"128","author":"K.M. Gehrs","year":"2009","unstructured":"Gehrs, K.M., Jackson, J.R., Brown, E.N., Allikmets, M., Hageman, G. (2009) Complement, age-related macular degeneration and a vision of the future. Arch. Ophthalmol. 128, 349\u2013358.","journal-title":"Arch. Ophthalmol."},{"key":"2_CR7","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1146\/annurev.pharmtox.47.120505.105225","volume":"47","author":"G.H. Travis","year":"2007","unstructured":"Travis, G.H., Golczak, M., Moise, A.R., Palczewski, K. (2007) Diseases caused by defects in the visual cycle: Retinoids as potential therapeutic agents. Annu. Rev. Pharmacol. Toxicol. 47, 469\u2013512.","journal-title":"Annu. Rev. Pharmacol. Toxicol"},{"key":"2_CR8","doi-asserted-by":"publisher","first-page":"395","DOI":"10.1016\/0014-4835(76)90177-9","volume":"22","author":"D. Bok","year":"1976","unstructured":"Bok, D., Heller, J. (1976) Transport of retinol from the blood to the retina: An autoradiographic study of the pigment epithelial cell surface receptor for plasma retinol-binding protein. Exp. Eye Res. 22, 395\u2013402.","journal-title":"Exp. Eye Res"},{"key":"2_CR9","doi-asserted-by":"publisher","first-page":"2025","DOI":"10.1172\/JCI105889","volume":"47","author":"M. Kanai","year":"1968","unstructured":"Kanai, M., Raz, A., Goodman, D.S. (1968) Retinol-binding protein: The transport protein for vitamin A in human plasma. J. Clin. Invest. 47, 2025\u20132044.","journal-title":"J. Clin. Invest"},{"key":"2_CR10","first-page":"1405","volume":"19","author":"D. Bok","year":"1980","unstructured":"Bok, D., Heller, J. (1980). Autoradiographic localization of serum retinol-binding protein receptors on the pigment epithelium of dystrophic rat retinas. Invest. Ophthalmol. Vis. Sci. 19, 1405\u20131414.","journal-title":"Invest. Ophthalmol. Vis. Sci"},{"key":"2_CR11","doi-asserted-by":"publisher","first-page":"820","DOI":"10.1126\/science.1136244","volume":"315","author":"R. Kawaguchi","year":"2007","unstructured":"Kawaguchi, R., Yu, J., Honda, J., et al. (2007) A membrane receptor for retinol binding protein mediates cellular uptake of Vitamin A. Science 315, 820\u2013825.","journal-title":"Science"},{"key":"2_CR12","doi-asserted-by":"publisher","first-page":"1179","DOI":"10.1086\/518177","volume":"80","author":"C. Golzio","year":"2007","unstructured":"Golzio, C., Martinovic-Bouriel, J., Thomas, S., et al. (2007) Matthew-Wood syndrome is caused by truncating mutations in the retinol-binding protein receptor gene STRA6. Am. M. Hum. Genet. 80, 1179\u20131187.","journal-title":"Am. M. Hum. Genet"},{"key":"2_CR13","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1086\/512203","volume":"80","author":"F. Pasutto","year":"2007","unstructured":"Pasutto, F., Sticht, H., Hammersen, G., et al. (2007) Mutations in STRA6 cause a broad spectrum of malformations including anophthalmia, congenital heart defects, diaphragmatic hernia, alveolar capillary dysplasia, lung hypoplasia and mental retardation. Am. J. Hum. Genet. 80, 550\u2013560.","journal-title":"Am. J. Hum. Genet"},{"key":"2_CR14","doi-asserted-by":"crossref","first-page":"12478","DOI":"10.1016\/S0021-9258(18)37779-2","volume":"263","author":"P.N. MacDonald","year":"1988","unstructured":"MacDonald, P.N., Ong, D.E. (1988) Evidence for a lecithin-retinol acyltransferase activity in the rat small intestine. J. Biol. Chem. 263, 12478\u201312482.","journal-title":"J. Biol. Chem"},{"key":"2_CR15","doi-asserted-by":"crossref","first-page":"8636","DOI":"10.1016\/S0021-9258(18)81839-7","volume":"264","author":"J. Saari","year":"1989","unstructured":"Saari, J., Bredberg, L. (1989) Lecithin: Retinolacyltransferase in retinal pigment epithelial microsomes. J. Biol. Chem. 264, 8636\u20138648.","journal-title":"J. Biol. Chem"},{"key":"2_CR16","doi-asserted-by":"publisher","first-page":"3834","DOI":"10.1074\/jbc.274.6.3834","volume":"274","author":"A. Ruiz","year":"1999","unstructured":"Ruiz, A., Winston, A., Rando, R., Bok, D. (1999) Molecular and biochemical characterization of lecithin retinol acyltransferase. J. Biol. Chem. 274, 3834\u20133841.","journal-title":"J. Biol. Chem"},{"key":"2_CR17","doi-asserted-by":"crossref","first-page":"16848","DOI":"10.1016\/S0021-9258(18)45461-0","volume":"262","author":"P. Bernstein","year":"1987","unstructured":"Bernstein, P., Law, W., Rando, R. (1987) Biochemical characterization of the retinoid isomerase4 system of the eye. J. Biol. Chem. 262, 16848\u201316857.","journal-title":"J. Biol. Chem"},{"key":"2_CR18","doi-asserted-by":"publisher","first-page":"968","DOI":"10.1126\/science.2727688","volume":"244","author":"P. Deigner","year":"1989","unstructured":"Deigner, P., Law, W., Canada, F., Rando, R. (1989) Membranes as the energy source in the undergone transformation of vitamin A to 11-cis-retinol. Science 244, 968\u2013971.","journal-title":"Science"},{"key":"2_CR19","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1016\/j.cell.2005.06.042","volume":"122","author":"M. Jin","year":"2005","unstructured":"Jin, M., Moghrabi, W.N., Sun, H., Travis, G.H. (2005) Rpe65 is the retinoid isomerase in bovine retinal pigment epithelium. Cell 122, 449\u2013459.","journal-title":"Cell"},{"key":"2_CR20","doi-asserted-by":"publisher","first-page":"12413","DOI":"10.1073\/pnas.0503460102","volume":"102","author":"G. Moiseyev","year":"2005","unstructured":"Moiseyev, G., Chen, Y., Takahashi, Y., Wu, B.X., Ma, J.X. (2005) RPE65 is the isomerhydrolase in the retinoid visual cycle. Proc. Nat. Acad. Sci. USA 102, 12413\u201312418.","journal-title":"Proc. Nat. Acad. Sci. USA"},{"key":"2_CR21","first-page":"189","volume":"17","author":"D. Bok","year":"1994","unstructured":"Bok, D. (1994) The retinal pigment epithelium; a versatile partner in vision. J. Cell Sci. 17(Suppl), 189\u2013195.","journal-title":"J. Cell Sci."},{"key":"2_CR22","doi-asserted-by":"publisher","first-page":"13658","DOI":"10.1073\/pnas.0504167102","volume":"102","author":"R.M. Redmond","year":"2005","unstructured":"Redmond, R.M., Poliakov, E., Yu, S., Tsai, J.Y., Lu, Z., Gentlemen, S. (2005) Mutation of key residues of ROE65 abolishes its enzymatic role as isomerohydrolase in the visual cycle. Proc. Natl. Acad. Sci. USA 102, 13658\u201313663.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2_CR23","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1038\/77004","volume":"25","author":"L.L. Molday","year":"2000","unstructured":"Molday, L.L., Rabin, A.R., Molday, R.S. (2000) ABCR expression in foveal cone photoreceptors and its role in Stargardt macular dystrophy. Nat. Genet. 25, 67\u201373.","journal-title":"Nat. Genet"},{"key":"2_CR24","doi-asserted-by":"publisher","first-page":"724","DOI":"10.1038\/361724a0","volume":"361","author":"G.E. Eldred","year":"1993","unstructured":"Eldred, G.E., Lasky, M.R. (1993) Retinal age pigments generated by self-assembling lysosomotropic detergents. Nature 361, 724\u2013726.","journal-title":"Nature"},{"key":"2_CR25","doi-asserted-by":"publisher","first-page":"2240","DOI":"10.1056\/NEJMoa0802315","volume":"358","author":"A.M. Maguire","year":"2008","unstructured":"Maguire, A.M., Simonelli, F., Pierce, E.A. et al. (2008) Safety and efficacy of gene transfer for Leber\u2019s congenital Amaurosis. N. Engl. J. Med. 358, 2240\u20132248.","journal-title":"N. Engl. J. Med"},{"key":"2_CR26","doi-asserted-by":"publisher","first-page":"2231","DOI":"10.1056\/NEJMoa0802268","volume":"358","author":"J.W. Bainbridge","year":"2008","unstructured":"Bainbridge, J.W., Smith, A.J., Barker, S.S., et al. (2008) Effect of gene therapy on visual function in Leber\u2019s congenital Amaurosis. N. Engl. J. Med. 358, 2231\u20132239.","journal-title":"N. Engl. J. Med"},{"key":"2_CR27","doi-asserted-by":"publisher","first-page":"979","DOI":"10.1089\/hum.2008.107","volume":"19","author":"W.W. Hauswirth","year":"2008","unstructured":"Hauswirth, W.W., Aleman, T.S., Causal, S., et al. (2008) Treatment of Leber congenital amaurosis due to RPE65 mutations by ocular subretinal injection of adeno-associated virus gene vector: Short-term results of a phase I trial. Hum. Gene Ther. 19, 979\u2013990.","journal-title":"Hum. Gene Ther"},{"key":"2_CR28","doi-asserted-by":"publisher","first-page":"16182","DOI":"10.1073\/pnas.0604255103","volume":"103","author":"J. Zhou","year":"2006","unstructured":"Zhou, J., Jang, P., Kim, S.R., Sparrow, J.R. (2006) Complement activation by photooxidation products of A2E, a lipofuscin constituent of the retinal pigment epithelium. Proc. Natl. Acad. Sci. USA 103, 16182\u201316187.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2_CR29","doi-asserted-by":"publisher","first-page":"7227","DOI":"10.1073\/pnas.0501536102","volume":"102","author":"G.S. Hageman","year":"2005","unstructured":"Hageman, G.S., Anderson, D.H. Johnson, L.V., et al. (2005) A common haplotype in the complement regulatory gene factor H (HF1\/CFH) predisposes individuals to age-related macular degeneration. Proc. Natl. Acad. Sci. USA 102, 7227\u20137232.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2_CR30","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1126\/science.1110359","volume":"308","author":"J.L. Haines","year":"2005","unstructured":"Haines, J.L., Hauser, M.A., Schmidt, S., et al. (2005) Complement factor H variant increases the risk of age-related macular degeneration. Science 308, 419\u2013421.","journal-title":"Science"},{"key":"2_CR31","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1126\/science.1109557","volume":"308","author":"R.J. Klein","year":"2005","unstructured":"Klein, R.J., Zeiss, D., Chew, E.Y., et al. (2005) Complement factor H polymorphism in age-related macular degeneration. Science 308, 385\u2013389.","journal-title":"Science"},{"key":"2_CR32","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1126\/science.1110189","volume":"308","author":"A.O. Edwards","year":"2005","unstructured":"Edwards, A.O., Ritter, R., 3rd, Abel, K.J., Manning, A., Panhuysen, C. Farrer, L.A. (2005) Complement factor H polymorphism and age-related macular degeneration. Science 308, 421\u2013424.","journal-title":"Science"},{"key":"2_CR33","doi-asserted-by":"crossref","unstructured":"Carlson, A., Bok, D. Promotion of the release of 11-cis-retinal from cultured retinal pigment epithelium by interphotoreceptor retinoid-binding protein. Biochemistry 31, 9056\u20139062.","DOI":"10.1021\/bi00152a049"},{"key":"2_CR34","doi-asserted-by":"publisher","first-page":"19730","DOI":"10.1074\/jbc.M801288200","volume":"283","author":"R.A. Radu","year":"2008","unstructured":"Radu, R.A., Hu, J., Peng, J., Bok, D., Mata, N., Travis, G.H. (2008) Retinal pigment epithelium-retinal G protein receptor-opsin mediates light-dependent translocation of all-trans-retinyl esters for synthesis of visual chromophore in retinal pigment epithelial cells. J. Biol. Chem. 283, 19730\u201319738.","journal-title":"J. Biol. Chem"},{"key":"2_CR35","doi-asserted-by":"publisher","first-page":"3612","DOI":"10.1167\/iovs.05-1622","volume":"47","author":"A. Maminishkis","year":"2006","unstructured":"Maminishkis, A., Chen, S., Jalickee, S., et al. (2006) Confluent monolayers of cultured human fetal retinal pigment epithelium exhibit morphology and physiology of native tissue. Invest. Ophthalmol. Vis. Sci. 47, 3612\u20133624.","journal-title":"Invest. Ophthalmol. Vis. Sci"}],"container-title":["Methods in Molecular Biology","Retinoids"],"original-title":[],"link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-60327-325-1_2","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,28]],"date-time":"2021-10-28T10:43:23Z","timestamp":1635417803000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-60327-325-1_2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010]]},"ISBN":["9781603273244","9781603273251"],"references-count":35,"URL":"https:\/\/doi.org\/10.1007\/978-1-60327-325-1_2","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010]]}}}