{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:22:50Z","timestamp":1790616170493,"version":"4.1.0"},"publisher-location":"Totowa, NJ","reference-count":17,"publisher":"Humana Press","isbn-type":[{"value":"9781603273640","type":"print"},{"value":"9781603273657","type":"electronic"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010]]},"DOI":"10.1007\/978-1-60327-365-7_3","type":"book-chapter","created":{"date-parts":[[2010,4,7]],"date-time":"2010-04-07T06:55:38Z","timestamp":1270623338000},"page":"39-52","source":"Crossref","is-referenced-by-count":11,"title":["Clostridium difficile Isolation and Culture Techniques"],"prefix":"10.1007","author":[{"given":"Mike","family":"Wren","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2010,3,8]]},"reference":[{"key":"3_CR1","doi-asserted-by":"publisher","first-page":"989","DOI":"10.1046\/j.1469-0691.2003.00683.x","volume":"9","author":"F Barbut","year":"2003","unstructured":"Barbut F, Delmee M, Brazier JS, Petit JC, Poxton IR, Rupnik M, Lalande V, Schneider C, Mastrantonio P, Alonso R, Kuiper E and Tvede M. (2003) ESCMID Study Group on Costridium difficile (ESGD). A European survey of diagnostic methods and testing protocols for Clostridium difficile. Clin Microbiol Infect\n                  9, 989\u2013996.","journal-title":"Clin Microbiol Infect"},{"key":"3_CR2","doi-asserted-by":"publisher","first-page":"667","DOI":"10.1128\/JCM.41.2.667-670.2003","volume":"41","author":"DK Turgeon","year":"2003","unstructured":"Turgeon DK, Novicki TJ, Quick J, Carlsson L, Miller P, Ulness B, Cent A, Ashley R, Coyle M, Limaye AP, Cookson BT and Fritsche TR. (2003) Six rapid tests for direct detection of Clostridium difficile and its toxins in fecal samples compared with the fibroblast cytotoxicity assay. J Clin Microbiol\n                  41, 667\u2013670.","journal-title":"J Clin Microbiol"},{"key":"3_CR3","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1309\/U8ATL52Q60XYAVX6","volume":"119","author":"V Massey","year":"2003","unstructured":"Massey V, Gregson DB, Chagla AH, Storey M, John MA and Hussain Z. (2003) Clinical usefulness of components of the Triage immunoassay, enzyme immunoassay for toxins A and B, and cytotoxin B tissue culture assay for the diagnosis of Clostridium difficile diarrhoea. Am J Clin Pathol\n                  119, 45\u201349.","journal-title":"Am J Clin Pathol"},{"key":"3_CR4","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1099\/jmm.0.45844-0","volume":"54","author":"M Delmee","year":"2005","unstructured":"Delmee M, Van Broeck J, Simon A, Janssens M and Avensi V. (2005) Laboratory diagnosis of Clostridium difficile-associated diarrhoea: a plea for culture. J Med Microbiol\n                  54, 187\u2013191.","journal-title":"J Med Microbiol"},{"key":"3_CR5","doi-asserted-by":"publisher","first-page":"669","DOI":"10.1016\/S1542-3565(04)00290-3","volume":"2","author":"MS Morelli","year":"2004","unstructured":"Morelli MS, Rouster SD, Giannella RA and Sherman KE. (2004) Clinical application of polymerase chain reaction to diagnose Clostridium difficile in hospitalised patients with diarrhoea. Clin Gastroenterol Hepatol\n                  2, 669\u2013674.","journal-title":"Clin Gastroenterol Hepatol"},{"key":"3_CR6","doi-asserted-by":"publisher","first-page":"5338","DOI":"10.1128\/JCM.43.10.5338-5340.2005","volume":"43","author":"RJ berg van den","year":"2005","unstructured":"van den berg RJ, Bruijnesteijn van Coppenraet LS, Gerritsen HJ, Endtz HP, van der Vorm ER and Kuijper EJ. (2005) Prospective multicentre evaluation of a new immunoassay and real-time PCR for rapid diagnosis of Clostridium difficile-associated diarrhoea in hospitalised patients. J Clin Microbiol\n                  43, 5338\u20135340.","journal-title":"J Clin Microbiol"},{"key":"3_CR7","first-page":"1124","volume":"18","author":"SP Borriello","year":"1981","unstructured":"Borriello SP and Honour P. (1981) Simplified procedure for routine isolation of Clostridium difficile from faeces. J Clin Microbiol\n                  18, 1124\u20131127.","journal-title":"J Clin Microbiol"},{"key":"3_CR8","first-page":"207","volume-title":"Anaerobic Pathogens","author":"NM Al-Saif","year":"1997","unstructured":"Al-Saif NM, Brazier JS and Duerden BI. (1997) Environmental studies of Clostridium difficile. In: Eley AR and Bennett KW eds., Anaerobic Pathogens, Sheffield Academic Press, London, 207\u2013213."},{"key":"3_CR9","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1053\/jhin.1999.0253","volume":"44","author":"MH Wilcox","year":"2000","unstructured":"Wilcox MH, Fawley WN and Parnell P. (2000) Value of lysozyme agar incorporation and alkaline thioglycollate exposure for the environmental recovery of Clostridium difficile. J Hosp Infect\n                  44, 65\u201369.","journal-title":"J Hosp Infect"},{"key":"3_CR10","doi-asserted-by":"publisher","first-page":"485","DOI":"10.3201\/eid1303.060988","volume":"13","author":"A Rodriguez-Palacios","year":"2007","unstructured":"Rodriguez-Palacios A, Staemfli HR, Duffield T and Weese JS. (2007) Clostridium difficile in retail ground meat, Canada. Emerging Infect Dis\n                  13, 485\u2013487.","journal-title":"Emerging Infect Dis"},{"key":"3_CR11","doi-asserted-by":"publisher","first-page":"328","DOI":"10.1128\/JCM.01503-07","volume":"46","author":"L Fenner","year":"2008","unstructured":"Fenner L, Widmer AF, Goy G, Rudin S and Frei R. (2008) Reliable diagnostic algorithm for detection of Clostridium difficile. J Clin Microbiol\n                  46, 328\u2013330.","journal-title":"J Clin Microbiol"},{"key":"3_CR12","first-page":"288","volume-title":"Clostridium difficile: Its Role in Intestinal Disease","author":"MAC Edelstein","year":"1988","unstructured":"Edelstein MAC. (1988) Isolation and identification of Clostridium difficile; tissue culturecytotoxicity assay. In: Rolfe RD and Finegold SM eds., Clostridium difficile: Its Role in Intestinal Disease, Academic Press, London, 288\u2013307."},{"key":"3_CR13","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1136\/jcp.56.2.126","volume":"56","author":"J Freeman","year":"2003","unstructured":"Freeman J and Wilcox MH. (2003) The effects of storage conditions on viability of Clostridium difficile vegetative cells and spores and toxin activity in human faeces. J Clin Pathol\n                  56, 126\u2013128.","journal-title":"J Clin Pathol"},{"key":"3_CR14","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1016\/j.jhin.2007.08.004","volume":"67","author":"MWD Wren","year":"2007","unstructured":"Wren MWD, Coen PG and Shetty NP. (2007) What is the true burden of Clostridium difficile disease? J Hosp Infect\n                  67, 196\u2013197.","journal-title":"J Hosp Infect"},{"key":"3_CR15","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1016\/0041-0101(92)90466-I","volume":"30","author":"O Baldacini","year":"1992","unstructured":"Baldacini O, Girardot R, Green GA, Rihn B and Montiel H. (1992) Comparative study of immunological properties and cytotoxic effects of Clostridium difficile toxin B and Clostridium sordellii toxin L. Toxicon\n                  30, 129\u2013140.","journal-title":"Toxicon"},{"key":"3_CR16","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1099\/jmm.0.46680-0","volume":"56","author":"R Berg van den","year":"2007","unstructured":"van den Berg R, Vaessen N, Endtz HP, Schulin T, van der Norm ER and Kuijper E. (2007) Evaluation of real-time PCR and conventional diagnostic methods for the detection of Clostridium difficile-associated diarrhoea in a prospective multicentre study. J Med Microbiol\n                  56, 36\u201342.","journal-title":"J Med Microbiol"},{"key":"3_CR17","doi-asserted-by":"publisher","first-page":"1996","DOI":"10.1128\/JCM.00032-08","volume":"46","author":"LM Sloan","year":"2008","unstructured":"Sloan LM, Duresko BJ, Gustafson DR and Rosenblatt JE. (2008) Comparison of real-time PCR for detection of the tcdC gene with four immunoassays and culture in the diagnosis of Clostridium difficile infection. J Clin Microbiol\n                  46, 1996\u20132001.","journal-title":"J Clin Microbiol"}],"container-title":["Methods in Molecular Biology","Clostridium difficile"],"original-title":[],"link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-60327-365-7_3","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,3,15]],"date-time":"2019-03-15T03:25:37Z","timestamp":1552620337000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-60327-365-7_3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010]]},"ISBN":["9781603273640","9781603273657"],"references-count":17,"URL":"https:\/\/doi.org\/10.1007\/978-1-60327-365-7_3","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010]]}}}