{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,11]],"date-time":"2026-11-11T11:54:33Z","timestamp":1794398073730,"version":"4.9.0"},"publisher-location":"Totowa, NJ","reference-count":21,"publisher":"Humana Press","isbn-type":[{"value":"9781617793240","type":"print"},{"value":"9781617793257","type":"electronic"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012]]},"DOI":"10.1007\/978-1-61779-325-7_16","type":"book-chapter","created":{"date-parts":[[2011,9,28]],"date-time":"2011-09-28T13:06:29Z","timestamp":1317215189000},"page":"199-218","source":"Crossref","is-referenced-by-count":14,"title":["Detection of Vaccinia Virus-Specific IFN\u03b3 and IL-10 Secretion from Human PBMCs and CD8+ T Cells by ELISPOT"],"prefix":"10.1007","author":[{"given":"Benjamin J.","family":"Umlauf","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norman A.","family":"Pinsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inna G.","family":"Ovsyannikova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory A.","family":"Poland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2011,9,2]]},"reference":[{"key":"16_CR1_16","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.coi.2009.04.004","volume":"21","author":"R.B. Kennedy","year":"2009","unstructured":"Kennedy, R.B., Ovsyannikova, I.G., Jacobson, R.M., and Poland, G.A. (2009)The immunology of smallpox vaccines. Curr Opin Immunol 21, 314\u2013320.","journal-title":"Curr Opin Immunol"},{"key":"16_CR2_16","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1038\/nm916","volume":"9","author":"R Weltzin","year":"2003","unstructured":"Weltzin, R,. Liu, J., Pugachev, K.V., Myers, G.A., Coughlin, B., Blum, P.S., et al. (2003) Clonal vaccinia virus grown in cell culture as a new smallpox vaccine. Nat Med 9, 1125\u20131130.","journal-title":"Nat Med"},{"key":"16_CR3_16","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1016\/j.vaccine.2005.01.012","volume":"23","author":"G.A. Poland","year":"2005","unstructured":"Poland, G.A., Grabenstein, J.D., Neff, J.M. (2005) The US smallpox vaccination program: a review of a large modern era smallpox vaccination implementation program. Vaccine 23, 2078\u20132081.","journal-title":"Vaccine"},{"key":"16_CR4_16","doi-asserted-by":"crossref","first-page":"1962","DOI":"10.1099\/vir.0.010553-0","volume":"90","author":"I.K. Damon","year":"2009","unstructured":"Damon, I.K., Davidson, W.B., Hughes, C.M., Olson, V.A., Smith, S.K., Holman, R.C., et al. (2009) Evaluation of smallpox vaccines using variola neutralization. J Gen Virol 90, 1962\u20131966.","journal-title":"J Gen Virol"},{"key":"16_CR5_16","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1128\/CVI.00109-09","volume":"16","author":"R.B. Kennedy","year":"2009","unstructured":"Kennedy, R.B., Pankratz, V.S., Swanson, E., Watson, D., Golding, H., and Poland, G.A. (2009) Statistical approach to estimate vaccinia- specific neutralizing antibody titers using a high throughput assay. Clin Vaccine Immunol 16, 1105\u20131112.","journal-title":"Clin Vaccine Immunol"},{"key":"16_CR6_16","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1056\/NEJMoa013431","volume":"346","author":"S.E. Frey","year":"2002","unstructured":"Frey, S.E., Newman, F.K., Cruz, J., et al. (2002) Dose-related effects of smallpox vaccine. N Engl J Med 346, 1275\u201380.","journal-title":"N Engl J Med"},{"key":"16_CR7_16","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.1128\/CVI.00181-06","volume":"13","author":"S.H. Kim","year":"2006","unstructured":"Kim, S.H., Yeo, S.G., Cho, J.H., et al. (2006) Cell-mediated immune responses to smallpox vaccination. Clin Vaccine Immunol 13, 1172\u20131174.","journal-title":"Clin Vaccine Immunol"},{"key":"16_CR8_16","doi-asserted-by":"crossref","first-page":"3723","DOI":"10.4049\/jimmunol.164.7.3723","volume":"164","author":"T. Helms","year":"2000","unstructured":"Helms, T., Boehm, B.O., Asaad, R.J., Trezza, R.P., Lehmann, P.V., and Tary-Lehmann, M. (2000) Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients. J Immunol 164, 3723\u20133732.","journal-title":"J Immunol"},{"key":"16_CR9_16","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/0022-1759(88)90079-8","volume":"110","author":"C. Czerkinsky","year":"1988","unstructured":"Czerkinsky, C., Andersson, G., Ekre, H.P., Nilsson, L.A., Klareskog, L., and Ouchterlony, O. (1988) Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells. J Immunol Methods 110, 29\u201336.","journal-title":"J Immunol Methods"},{"key":"16_CR10_16","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.jim.2008.11.001","volume":"341","author":"J.E. Ryan","year":"2009","unstructured":"Ryan, J.E., Dhiman, N., Ovsyannikova, I.G., Vierkant, R.A., Pankratz, V.S., and Poland, G.A. (2009) Response surface methodology to determine optimal cytokine responses in human peripheral blood mononuclear cells after smallpox vaccination. J Immunol Methods 341, 97\u2013105.","journal-title":"J Immunol Methods"},{"key":"16_CR11_16","first-page":"16","volume":"16","author":"P.L. Earl","year":"2001","unstructured":"Earl, P.L., Moss, B., Wyatt, L.S., and Carroll, M.W. (2001) Generation of recombinant vaccinia viruses. Curr Protoc Mol Biol Chapter 16, Unit 16.","journal-title":"Curr Protoc Mol Biol"},{"key":"16_CR12_16","doi-asserted-by":"crossref","unstructured":"Earl, P.L., Cooper, N., Wyatt, L.S., Moss, B., and Carroll, M.W. (2001) Preparation of cell cultures and vaccinia virus stocks. Curr Protoc Mol Biol Chapter 16, Unit 16.","DOI":"10.1002\/0471142727.mb1616s43"},{"key":"16_CR13_16","doi-asserted-by":"crossref","first-page":"3154","DOI":"10.1128\/JCM.41.7.3154-3157.2003","volume":"41","author":"F.K. Newman","year":"2003","unstructured":"Newman, F.K., Frey, S.E., Blevins, T.P., Mandava, M., Bonifacio, A. Jr., Yan, L., et al. (2003) Improved assay to detect neutralizing antibody following vaccination with diluted or undiluted vaccinia (Dryvax) vaccine. J Clin Microbiol 41, 3154\u20133157.","journal-title":"J Clin Microbiol"},{"key":"16_CR14_16","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1128\/JVI.70.1.165-171.1996","volume":"70","author":"K. Tsung","year":"1996","unstructured":"Tsung, K., Yim, J.H., Marti, W., Buller, R.M., and Norton, J.A. (1996) Gene expression and cytopathic effect of vaccinia virus inactivated by psoralen and long-wave UV light. J Virol 70, 165\u2013171.","journal-title":"J Virol"},{"key":"16_CR15_16","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0022-1759(03)00226-6","volume":"278","author":"C.R. Kreher","year":"2003","unstructured":"Kreher, C.R., Dittrich, M.T., Guerkov, R., Boehm, B.O., and Tary-Lehmann, M. (2003) CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays. J Immunol Methods 2003 278, 79\u201393.","journal-title":"J Immunol Methods"},{"key":"16_CR16_16","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1128\/CDLI.8.5.871-879.2001","volume":"8","author":"J.G. Smith","year":"2001","unstructured":"Smith, J.G., Liu, X., Kaufhold, R.M., Clair, J., and Caulfield, M.J. (2001) Development and validation of a gamma interferon ELISPOT assay for quantitation of cellular immune responses to varicella-zoster virus. Clin Diagn Lab Immunol 8, 871\u2013879.","journal-title":"Clin Diagn Lab Immunol"},{"key":"16_CR17_16","doi-asserted-by":"crossref","first-page":"3296","DOI":"10.1073\/pnas.94.7.3296","volume":"94","author":"P. Salomoni","year":"1997","unstructured":"Salomoni, P., Perrotti, D., Martinez, R., Franceschi, C., and Calabretta, B. (1997) Resistance to apoptosis in CTLL-2 cells constitutively expressing c-Myb is associated with induction of BCL-2 expression and Myb-dependent regulation of bcl-2 promoter activity. Proc Natl Acad Sci U S A 94, 3296\u20133301.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"16_CR18_16","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1126\/science.3523754","volume":"233","author":"J.B. Stern","year":"1986","unstructured":"Stern, J.B., and Smith, K.A. (1986) Interleukin-2 induction of T-cell G1 progression and c-myb expression. Science 233, 203\u2013206.","journal-title":"Science"},{"key":"16_CR19_16","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1016\/j.jaci.2009.02.011","volume":"123","author":"S. Letourneau","year":"2009","unstructured":"Letourneau, S., Krieg, C., Pantaleo, G., and Boyman, O. (2009) IL-2- and CD25-dependent immunoregulatory mechanisms in the homeostasis of T-cell subsets. J Allergy Clin Immunol 123, 758\u2013762.","journal-title":"J Allergy Clin Immunol"},{"key":"16_CR20_16","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1089\/aid.2006.0129","volume":"23","author":"L.S. Kierstead","year":"2007","unstructured":"Kierstead, L.S., Dubey, S., Meyer, B., Tobery, T.W., Mogg, R., Fernandez, V.R., et al. (2007) Enhanced rates and magnitude of immune responses detected against an HIV vaccine: effect of using an optimized process for isolating PBMC. AIDS Res Hum Retroviruses 23, 86\u201392.","journal-title":"AIDS Res Hum Retroviruses"},{"key":"16_CR21_16","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S0022-1759(01)00451-3","volume":"257","author":"A. Kalyuzhny","year":"2001","unstructured":"Kalyuzhny, A., and Stark, S. (2001) A simple method to reduce the background and improve well-to-well reproducibility of staining in ELISPOT assays. J Immunol Methods 257, 93\u201397.","journal-title":"J Immunol Methods"}],"container-title":["Methods in Molecular Biology","Handbook of ELISPOT"],"original-title":[],"link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-61779-325-7_16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,6,25]],"date-time":"2020-06-25T01:20:54Z","timestamp":1593048054000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-61779-325-7_16"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,9,2]]},"ISBN":["9781617793240","9781617793257"],"references-count":21,"URL":"https:\/\/doi.org\/10.1007\/978-1-61779-325-7_16","relation":{"is-part-of":[{"id-type":"doi","id":"https:\/\/doi.org\/10.1007\/978-1-61779-325-7","asserted-by":"object"}]},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,9,2]]}}}